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2株枯草芽孢杆菌对大肠杆菌和沙门氏菌的体外抑菌试验研究
引用本文:秦瑶,王苇,郭秉娇,王熙楚,张文举,周霞,王晓兰.2株枯草芽孢杆菌对大肠杆菌和沙门氏菌的体外抑菌试验研究[J].中国畜牧兽医,2014,41(1):207-210.
作者姓名:秦瑶  王苇  郭秉娇  王熙楚  张文举  周霞  王晓兰
作者单位:(石河子大学动物科技学院,新疆石河子 832003)
基金项目:石河子大学重大科技攻关项目(gxjs2011-zdgg01-04);国家自然基金(31160507)。
摘    要:本试验旨在检测2株枯草芽孢杆菌对致病性大肠杆菌和沙门氏菌抑菌作用。以致病性大肠杆菌和沙门氏菌作为指示菌,采用2株枯草芽孢杆菌与大肠杆菌和沙门氏菌同时接种及先后接种的方法,对其与大肠杆菌和沙门氏菌的生物颉颃作用进行了研究。结果表明,先接种枯草芽孢杆菌再接种致病菌抑制作用最明显且在24 h时抑菌作用最强,同时接种枯草芽孢杆菌和致病菌抑制作用次之,先接种致病菌再接种枯草芽孢杆菌的抑制作用最弱。提示,2株枯草芽孢杆菌对致病性大肠杆菌和沙门氏菌都有一定的抑制作用,但抑制作用的强弱有差异,且与枯草芽孢杆菌和致病菌接种的时间长短有关。

关 键 词:枯草芽孢杆菌  大肠杆菌  沙门氏菌  抑菌试验  

Study on Bacillus subtilis Strains Q4 and Q10 Inhibiting the Growth of E.coli and Salmonella in vitro
QIN Yao,WANG Wei,GUO Bing-jiao,WANG Xi-chu,ZHANG Wen-ju,ZHOU Xia,WANG Xiao-lan.Study on Bacillus subtilis Strains Q4 and Q10 Inhibiting the Growth of E.coli and Salmonella in vitro[J].China Animal Husbandry & Veterinary Medicine,2014,41(1):207-210.
Authors:QIN Yao  WANG Wei  GUO Bing-jiao  WANG Xi-chu  ZHANG Wen-ju  ZHOU Xia  WANG Xiao-lan
Institution:(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)
Abstract:The effects of 2 Bacillus subtilis strains,Q4 and Q10 inhibiting the growth of E. coli and Salmonella.Regarded E.coli and Salmonella as indicators,investigated by the co-incubation, pre-incubation and post-incubation method.The results revealed that 2 Bacillus subtilis strains showed different ability to inhibit the growth of E. coli and Salmonella after incubating with Q4 and Q10, respectively.Having inoculated Bacillus subtilis and then co-incubation pathogens inhibition were most obvious in means of the strongest inhibitory effect at 24 h,at the same time,pre-incubation Bacillus subtilis and then co-incubation pathogen the inhibition was the weakest of all,the pathogens and Bacillus subtilis co-incubation were the second. From the former consequences that 2 Bacillus subtilis strains of Q4 and Q10 had inhibitory effect on the pathogen,but there were differences in inhibition,and related to the duration of Bacillus subtilis and pathogen inoculation.
Keywords:Bacillus subtilis  E  coli  Salmonella  bacteriostatic test
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