| 停乳链球菌内蒙分离株抗原基因MIG的克隆 |
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| 引用本文： | 刘金哗,白龙,郝永清,张爱荣.停乳链球菌内蒙分离株抗原基因MIG的克隆[J].中国动物保健,2011,13(5):18-21. |
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| 作者姓名： | 刘金哗 白龙 郝永清 张爱荣 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018 |
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| 摘 要： | 停乳链球菌是引起奶牛乳房炎的主要病原菌,根据GenBank中登陆的停乳链球菌表面蛋白MIG基因序列,设计一对引物,采用PCR的方法从临床分离的停乳链球菌内蒙分离株基因组DNA中扩增出MIG基因,得到一条1900bp的片段。将其连入PMD-19T载体中,经酶切,PCR及序列测定法进行鉴定。经DNA测序分析,证实与GenBank中停乳链球菌MIG基因(AF354651)序列同源性为95%,具有高度保守性。
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| 关 键 词： | 停乳链球菌 MIG基因 克隆 |
Antigen Gene MIG Cloning of S.dysgalactiae Isolated from Inner Mongolia |
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| Liu Jinye,Bai Long,Hao Yongqing,Zhang Airong.Antigen Gene MIG Cloning of S.dysgalactiae Isolated from Inner Mongolia[J].China Animal Health,2011,13(5):18-21. |
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| Authors: | Liu Jinye Bai Long Hao Yongqing Zhang Airong |
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| Institution: | ( College of Animal Science and Medicine, Inner Monglia Agricultural University, Huhhot, China) |
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| Abstract: | S.dysgalactiae is the major pathogens of mastitis in dairy cows. S.dysgalactiae were derived the mastitis bovine in some areas of Inner Mongolia. A pair of primers was designed according to S.dysgalactiae MIG surface protein gene sequences in GenBank.Subsequently, The MIG gene of 1900 bp was amplified by PCR. Then the gene was cloned into PMD-19T vector, identified by digestion with restriction endonucleases, PCR and sequencing. The DNA sequencing confirmed S.dysgalactiae in GenBank, MIG gene （AF354651） 95% homology. |
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| Keywords: | S dysgalactiae MIG gene clone |
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