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| 鹌鹑β-防御素10基因的克隆与表达及其体内分布的检测 | |
| 引用本文： | 蔺利娟,王瑞琴,韩宗玺,邵昱昊,刘胜旺,程宝晶,孙黎,李子瑞,马得莹.鹌鹑β-防御素10基因的克隆与表达及其体内分布的检测[J].中国预防兽医学报,2011,33(7). |
| 作者姓名： | 蔺利娟 王瑞琴 韩宗玺 邵昱昊 刘胜旺 程宝晶 孙黎 李子瑞 马得莹 |
| 作者单位： | 1. 东北农业大学 动物营养研究所,黑龙江哈尔滨 150030;中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨 150001 2. 中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨,150001 3. 东北农业大学 动物营养研究所,黑龙江哈尔滨,150030 |
| 基金项目： | 国家自然科学基金，黑龙江省教育厅重点项目 |
| 摘 要： | 为测定鹌鹑β-防御素10 (AvBD10)体外抗菌活性及检测其在鹌鹑脏器组织的分布,本研究应用RT-PCR方法从鹌鹑肺脏组织中扩增到鹌鹑AvBD10,经序列同源性分析,鹌鹑AvBD10与鸡AvBD10的氨基酸序列同源性最高(83.8％).将该基因亚克隆到表达载体pGEX-6p-1中进行原核表达,SDS-PAGE电泳表明,鹌鹑AvBD10重组蛋白分子量约为31ku.该重组蛋白经纯化后测定其体外抗菌活性,结果显示,鹌鹑AvBD10重组蛋白具有广谱的抗菌活性.采用定量RT-PCR法检测到AvBD10在鹌鹑脏器组织中广泛表达. |
| 关 键 词： | 鹌鹑 AvBD10 重组蛋白 抗菌活性:组织分布 |
Cloning and expression of quail avian beta-defensin 10 and the mRNA detection in quail tissues |
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| LIN Li-juan,WANG Rui-qin,HAN Zong-xi,SHAO Yu-hao,LIU Sheng-wang,CHENG Bao-jing,SUN Li,LI Zi-rui,MA De-ying.Cloning and expression of quail avian beta-defensin 10 and the mRNA detection in quail tissues[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(7). | |
| Authors: | LIN Li-juan WANG Rui-qin HAN Zong-xi SHAO Yu-hao LIU Sheng-wang CHENG Bao-jing SUN Li LI Zi-rui MA De-ying |
| Institution: | LIN Li-juan1,2,WANG Rui-qin1,HAN Zong-xi2,SHAO Yu-hao2,LIU Sheng-wang2,CHENG Bao-jing1,SUN Li1,LI Zi-rui1,MA De-ying1 (1. Institute of Animal Nutrition,Northeast Agricultural University,Harbin 150030,China,2. Division of Avian Infectious Disease,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
| Abstract: | To clone and express avian beta-defensin (AvBD10) gene in quail, the quail AvBD10 gene was amplified from lung of Chinese painted quail (Coturnix chinensis) by RT-PCR. Sequence analysis showed that the cDNA of quail AvBD10 consisted of 207 bp nucleotide, encoding a polypeptide of 68 amino acids. Homology analysis showed that quail AvBD10 shared 83.8% with chicken AvBD10. The cDNA of quail AvBD10 was sub-cloned into pGEX-6p-1 vector and expressed in E. coli BL21. SDS-PAGE analysis indicated that 31 ku of qua... |
| Keywords: | quail AvBD10 recombinant protein antimicrobial activity tissues distribution |
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