| 克伦特罗单克隆抗体的研制及初步鉴定 |
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| 引用本文： | 王帅兵,朱善元,袁宗辉.克伦特罗单克隆抗体的研制及初步鉴定[J].动物医学进展,2011,32(6):68-72. |
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| 作者姓名： | 王帅兵 朱善元 袁宗辉 |
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| 作者单位： | 王帅兵,WANG Shuai-bing(江苏畜牧兽医职业技术学院,江苏,泰州,225300);朱善元,ZHU Shan-yuan(江苏畜牧兽医职业技术学院,江苏,泰州,225300;江苏省兽用生物制药高技术研究重点实验室,江苏泰州,225300);袁宗辉,YUAN Zong-hui(农业部兽药残留基准实验室/农业部食品安全评价重点开放实验室,湖北武汉,430070) |
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| 基金项目： | 泰州市科技发展计划项目 |
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| 摘 要： | 为制备克伦特罗(clenbuterol,CL)单克隆抗体,鉴定其特性,采用重氮化法合成克伦特罗-人血清白蛋白(CL-HSA)作为免疫原免疫小鼠,通过杂交瘤技术建立杂交瘤细胞株,利用体内诱生法制备单克隆抗体,用间接ELISA和间接竞争ELISA分别测定抗体效价和特异性.将细胞反复冻存和传代,考察稳定性.获得细胞株C611...
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| 关 键 词： | 克伦特罗 单克隆抗体 效价 特异性 鉴定 |
Preparation and Identification of the Monoclonal Antibody Against Clenbuterol |
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| WANG Shuai-bing,ZHU Shan-yuan,YUAN Zong-hui.Preparation and Identification of the Monoclonal Antibody Against Clenbuterol[J].Progress In Veterinary Medicine,2011,32(6):68-72. |
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| Authors: | WANG Shuai-bing ZHU Shan-yuan YUAN Zong-hui |
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| Institution: | 1.Jiangsu Animal Husbandry and Veterinary College,Taizhou,Jiangsu,225300,China;2.Jiangsu Porvince Veterinary Bio-pharmaceutical High-tech Key Laboratory,Taizhou,Jiangsu,225300,China;3.Natinal Reference Laboratory of Veterinary Drug Residues∕MOA Key Laboratory of Food Safety Evaluation,Wuhan,Hubei,430070,China) |
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| Abstract: | To prepare monoclonal antibody against clenbuterol(CL) and identify its characteristics.Balb/c mice were immunized with the conjugate CL-HAS which had been prepared via a diazotization.The splenocytes of immunized mice were fused with Sp2/0 myeloma cells by a routine method.Monoclonal antibody(mAb) was raised in Balb/c mice and was identified by inditect ELISA for the titer,and complete indirect ELISA for its specificity.One stable hybridoma C611,secreting monoclonal antibodies against clenbuterol(CL) was established.It was highly specific for CL with a titer of 1∶64 000 in the ascitic fluid and showed low cross-reactivities less than 7% to other β-agonists.The limit detection of CL was 0.1 μg/L,and the IC50 was about 1 μg/L.The storage condition and cloning would not influence the property of the hybridomas.The mAb against CL were prepared successfully,and possessed high titer and specificity,which is useful for the development of an ELISA kit for screening of CL residue. |
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| Keywords: | clenbuterol monoclonal antibody titer specificity identification |
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