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| 安徽IBV地方株AH1-99株S2基因的克隆与序列分析 | |
| 引用本文： | 何长生,刘胜旺,魏建忠,朱良强,詹松鹤,江定丰,郑举.安徽IBV地方株AH1-99株S2基因的克隆与序列分析[J].动物医学进展,2006,27(8):98-101. |
| 作者姓名： | 何长生 刘胜旺 魏建忠 朱良强 詹松鹤 江定丰 郑举 |
| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001;安徽省兽医工作站,安徽合肥,230022 2. 中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨,150001 3. 安徽农业大学动物科技学院,安徽合肥,230036 4. 安徽省兽医工作站,安徽合肥,230022 |
| 摘 要： | 用RT-PCR技术扩增出IBVAH1-99株的S2基因cDNA，并将其克隆到pMD18-T载体中进行序列测定和分析。结果表明，该分离株S2基因全长共1881个核苷酸，编码625个氨基酸；与GenBank中登录的IBVS2基因核苷酸的同源性为72．5％～88．99，氨基酸的同源性为70．3%-88．0％；在895nt-900nt处插入了GCG ACT 6个碱基，在1441nt～1446nt处缺失了ATTACT6个碱基。 |
| 关 键 词： | 传染性支气管炎病毒 AH1-99株 S2基因 克隆 序列分析 |
| 文章编号： | 1007-5038（2006）08-0098-04 |
| 收稿时间： | 2006-04-14 |
| 修稿时间： | 2006-04-14 |
Cloning and Analysis of S2 Gene of Infectious bronchitis virus of Anhui Strain AH1-99 |
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| HE Chang-sheng,LIU Sheng-wang,WEI Jian-zhong,ZHU Liang-qiang,ZHAN Song-he,JIANG Ding-feng,ZHENG Ju.Cloning and Analysis of S2 Gene of Infectious bronchitis virus of Anhui Strain AH1-99[J].Progress In Veterinary Medicine,2006,27(8):98-101. | |
| Authors: | HE Chang-sheng LIU Sheng-wang WEI Jian-zhong ZHU Liang-qiang ZHAN Song-he JIANG Ding-feng ZHENG Ju |
| Institution: | 1. Harbin Veterinary Research Institute of CAAS, Harbin, Heilongjiang, 150001, China 2. Veterinary Service of Anhui Province, He fei ,Anhui , 230022, China 3. College of Animal Science, Anhui Agricultural University, Hefei,Anhui, 230036, China |
| Abstract: | S2 gene cDNA of IBV AH1-99 strain was amplified by RT-PCR. The S2 gene fragment was cloned into pMD18-T vector, and was sequenced and analyzed. The S2 gene was composed of 1881 nucleotides and coded 625 amino acid residues. Compared with other of S2 genes IBV strains in GenBank , the homology of S2 gene nucleotides of AH1-99 was between 72.5% to 88.9%, and the homology of amino acid was 70.3% to 88.9% There was an insertion of GCGACT at the site of nucleotide 895-900, and a deletion of ATTACT at the site of nucleotides 1 441-1 446. |
| Keywords: | IBV AH1-99 strain S2 gene clone sequence analysis |
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