| 玉米赤霉烯酮对鸡胚成纤维细胞的毒性作用 |
| |
| 引用本文： | 张凯照,胡会,许泽锴,王诗倩,崔红杰,黄小红.玉米赤霉烯酮对鸡胚成纤维细胞的毒性作用[J].畜牧兽医学报,2022,53(5):1615-1625. |
| |
| 作者姓名： | 张凯照 胡会 许泽锴 王诗倩 崔红杰 黄小红 |
| |
| 作者单位： | 1. 福建农林大学动物科学学院(蜂学学院), 福州 350002;2. 福建农林大学, 中西兽医结合与动物保健福建省高校重点实验室, 福州 350002;3. 福建农林大学, 福建省兽医中药与动物保健重点实验室, 福州 350002 |
| |
| 基金项目： | 福建省中青年教师教育科研项目(JAT200108)； |
| |
| 摘 要： | 旨在探究玉米赤霉烯酮(ZEA)对鸡胚成纤维细胞(DF-1)的毒性作用机制，采用MTT法检测细胞活力变化，比色法检测乳酸脱氢酶(LDH)活力，ELISA法检测Caspase-3含量，Annexin V-FITC/PI双染法检测细胞凋亡，荧光显微镜观察细胞活性氧(ROS)水平，线粒体膜电位变化，RT-qPCR法检测内质网应激(ERs)和细胞凋亡相关基因的mRNA转录水平。结果显示，12.5~50.0 μg·mL-1 ZEA显著抑制DF-1细胞增殖(P<0.01)，且呈时间和剂量依赖性关系。25.0 μg·mL-1 ZEA处理细胞24 h后，上清液中LDH和Caspase-3含量显著升高(P<0.01)；细胞中ROS水平和细胞凋亡数量极显著升高(P<0.01)；线粒体膜电位极显著降低(P<0.01)。凋亡相关基因Caspase-3、Bax mRNA转录水平极显著上调(P<0.01)，Bcl-2 mRNA转录水平极显著下调(P<0.01)；ERs相关基因GRP78、ATF6、ATF4、CHOP、PERK mRNA转录水平极显著上调(P<0.01)。综上表明，ZEA能通过内质网应激途径导致细胞凋亡并对鸡胚成纤维细胞发挥毒性作用。研究结果为深入研究ZEA对鸡细胞的毒性作用和解毒手段奠定基础，对相关禽类疾病治疗具有意义。
|
| 关 键 词： | 玉米赤霉烯酮 鸡胚成纤维细胞 细胞毒性 内质网应激 细胞凋亡 |
| 收稿时间： | 2021-09-08 |
Toxicity of Zearalenone on Chicken Embryo Fibroblasts |
| |
| ZHANG Kaizhao,HU Hui,XU Zekai,WANG Shiqian,CUI Hongjie,HUANG Xiaohong.Toxicity of Zearalenone on Chicken Embryo Fibroblasts[J].Acta Veterinaria et Zootechnica Sinica,2022,53(5):1615-1625. |
| |
| Authors: | ZHANG Kaizhao HU Hui XU Zekai WANG Shiqian CUI Hongjie HUANG Xiaohong |
| |
| Institution: | 1. College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fuzhou 350002, China;3. Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
| |
| Abstract: | This study was conducted to investigate the mechanism of the toxic effects of zearalenone (ZEA) on chicken embryo fibroblasts (DF-1). The cell viability, lactate dehydrogenase activity, Caspase-3 levels, cell apoptosis, cellular reactive oxygen species levels, mitochondrial membrane potential change and expression of endoplasmic reticulum stress (ERs) and apoptosis-related genes were detected by MTT, colorimetric assay, ELISA, Annexin V-FITC/PI staining, fluorescence microscopy and RT-qPCR, respectively. The results showed that 12.5-50.0 μg·mL-1 ZEA significantly inhibited the proliferation of DF-1 cells (P<0.01) in a time- and dose-dependent manner. Herein 25.0 μg·mL-1 ZEA treated cells for 24 h resulted in a significant increase in LDH and Caspase-3 levels in the supernatant (P<0.01); the levels of ROS and the number of apoptotic cells in the cells were highly significant (P<0.01); the mitochondrial membrane potential was significantly reduced (P<0.01). The mRNA expression levels of apoptosis-related genes Caspase-3 and Bax were highly significantly up-regulated (P<0.01) and that of Bcl-2 was highly significantly down-regulated (P<0.01); the mRNA expression levels of ERs-related genes GRP78, ATF6, ATF4, CHOP and PERK were highly significantly up-regulated (P<0.01). The results indicated that ZEA could exert toxic effects on chicken embryonic fibroblasts through endoplasmic reticulum stress leading to apoptosis. The results of the study provide a basis for further research on the toxic effects of ZEA on chicken cells and the means of detoxification, and have implications for the treatment of related avian diseases. |
| |
| Keywords: | zearalenone chicken embryo fibroblasts cytotoxicity endoplasmic reticulum stress apoptosis |
|
| 点击此处可从《畜牧兽医学报》浏览原始摘要信息 |
| 点击此处可从《畜牧兽医学报》下载免费的PDF全文 |
|
