| 抗大肠埃希氏菌粘附素单克隆抗体诊断试剂的研制及其现场初步应用 |
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| 引用本文： | 高崧,刘秀梵.抗大肠埃希氏菌粘附素单克隆抗体诊断试剂的研制及其现场初步应用[J].畜牧兽医学报,1993,24(3):245-253. |
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| 作者姓名： | 高崧 刘秀梵 |
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| 作者单位： | 江苏农学院畜牧兽医系
(高崧)，江苏农学院畜牧兽医系(刘秀梵) |
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| 摘 要： | 作者从所研制的抗产肠毒素性大肠埃希氏菌(ETEC)粘附素K_(88)、K_(99)、987P和F_(41)单克隆抗体(以下简称单抗)44株中的7株,建立了检测以上粘附素抗原的单抗诊断试剂,并确定了以4单抗和3单抗夹心ELISA为特征的检测大批量临床样品的诊断方法与程序,对1038例自然发生下痢仔猪粪样ETEC粘附素的检测结果表明,本试验所建立的诊断方法与程序,具有敏感、准确、快速和简便的特点。
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| 关 键 词： | 大肠埃希氏菌 粘附素 单克隆抗体 |
DIAGNOSTIC REAGENTS MABS BASED SPECIFIC FOR ETEC ADHESINS AND THEIR POTENTIAL USE IN THE DIAGNOSIS OF FIELD CASES |
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| Gao Song,Liu Xiufan.DIAGNOSTIC REAGENTS MABS BASED SPECIFIC FOR ETEC ADHESINS AND THEIR POTENTIAL USE IN THE DIAGNOSIS OF FIELD CASES[J].Acta Veterinaria et Zootechnica Sinica,1993,24(3):245-253. |
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| Authors: | Gao Song Liu Xiufan |
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| Abstract: | Seven out of 44 monoclonal antibodies (MAbs) with specificity to K88. K99. F41 and 987P antigens of enterotoxigenic escherichia coli. (ETEC) developed in our laboratory were selected to make up MAbs based diagnostic reagents, which were used for the detection of adhesins mentioned above. The diagnostic system which was characterized by 4 MAbs sandwich ELISA and 3 MAbs sandwich ELISA was also developed. The diagnostic system and protocals were evaluated for the detection of ETEC adhesins in specimens from 1038 natural cases of pig scour. It was confirmed the diagnostic system and protocals developed in this study had the advantages of sensitivity, accuracy, rapidity, and simplicity. |
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| Keywords: | Escherichia coli Adhesins Monoclonal antibodies Diagnstic reagents |
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