| 禽脑脊髓炎病毒单克隆抗体的制备与初步应用 |
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| 引用本文： | 徐建生,唐波,成大荣,董国雄,吕玲,曹军.禽脑脊髓炎病毒单克隆抗体的制备与初步应用[J].中国兽医科技,2006,36(2):89-92. |
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| 作者姓名： | 徐建生 唐波 成大荣 董国雄 吕玲 曹军 |
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| 作者单位： | 扬州大学兽医学院,江苏扬州225009 |
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| 摘 要： | 利用提纯的禽脑脊髓炎病毒（AEV）Van Roekel株作为免疫原，免疫6周龄BALB／c小鼠，采取其脾细胞与SP2／0骨髓瘤细胞融合，用间接ELISA法筛选，间接免疫荧光法（IFA）和免疫组织化学法鉴定，经3次亚克隆得到了稳定分泌抗AEV单克隆抗体的杂交瘤细胞株F11和G2，制备了腹水，并利用该单克隆抗体初步建立了Dot—ELISA、间接ELISA和IFA等特异性检测AEV抗原的方法。
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| 关 键 词： | 禽脑脊髓炎病毒 单克隆抗体 斑点-酶联免疫吸附法 |
| 文章编号： | 1673-4696（2006）02-0089-04 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-12-05 |
Preparation and preliminary application of monoclonal antibody to avian encephalomyelitis virus |
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| XU Jian-sheng, TANG Bo, CHENG Da-rong, DONG Guo-xiong, LUE Ling ,CAO Jun.Preparation and preliminary application of monoclonal antibody to avian encephalomyelitis virus[J].Chinese Journal of Veterinary Science and Technology,2006,36(2):89-92. |
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| Authors: | XU Jian-sheng TANG Bo CHENG Da-rong DONG Guo-xiong LUE Ling CAO Jun |
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| Abstract: | 6-week old BALB/c mice were immunized with purified avian encephalomyelitis viruses at two weeks interval. Mice were killed and their spleen cells fused into SP2/0 myeloma cells on day 3 after third immunization. Indirect-ELISA, indirect immunofluorescent assay and immunohistochemistry were employed to select and characterize the fused cells secreting antibody to AEV. As a result, two hybridoma cell lines steadily secreting antibody to AEV, named Fll and G2, were obtained after subcloning for three times. Ascites were prepared with the hybridoma and indirect fluorescent assay, indirect ELISA and Dot- ELISA were developed with the monoclonal antibody for the detection of AEV. |
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| Keywords: | avian encephalomyelitis virus monoclonal antibody Dot-ELISA |
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