| 武定鸡脂联素基因多态性分析 |
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| 引用本文： | 古旭,丁相红,陈粉粉.武定鸡脂联素基因多态性分析[J].畜牧与饲料科学,2020,41(2):17-19. |
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| 作者姓名： | 古旭 丁相红 陈粉粉 |
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| 作者单位： | 西南林业大学云南生物多样性研究院,云南昆明650224;西南林业大学生命科学学院,云南昆明650224 |
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| 基金项目： | 云南省特色优势重点学科生物学一级学科建设项目(50097505);云南省教育厅科学研究基金项目(2012Y230)。 |
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| 摘 要： | 目的]对武定鸡脂联素基因（NC_006096）第1外显子上的c.A99G突变位点进行多态性分析。方法]翅静脉采集67只武定鸡全血,提取基因组DNA,采用PCR-RFLP法分析Hsp92Ⅱ限制性内切酶对PCR产物进行单酶切的结果。结果]在c.A99G同义突变位点上存在AA、AB和BB 3种基因型,其中A基因频率为0.686 6,B基因频率为0.313 4;PIC=0.337 8,表明该位点为中度多态位点;χ2适合性检验表明,脂联素基因c.A99G位点处于平衡状态。结论]采用PCR-RFLP法可有效检测武定鸡脂联素基因c.A99G突变,该位点有3种基因型,等位基因呈中度遗传多态水平且处于平衡状态。
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| 关 键 词： | 武定鸡 脂联素基因 PCR-RFLP |
| 收稿时间： | 2020-02-07 |
Analysis of Polymorphism on Adiponectin Gene of Wuding Chicken |
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| GU Xu,DING Xiang-hong,CHEN Fen-fen.Analysis of Polymorphism on Adiponectin Gene of Wuding Chicken[J].Animal Husbandry and Feed Science,2020,41(2):17-19. |
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| Authors: | GU Xu DING Xiang-hong CHEN Fen-fen |
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| Institution: | 1.Yunnan Institute of Biodiversity,Southwest Forestry University,Kunming 650224,China; 2.College of Life Science,Southwest Forestry University,Kunming 650224,China |
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| Abstract: | Objective]To analyze the polymorphism of c.A99G mutation site on exon 1 of adiponectin gene(NC_006096)of Wuding chicken.Method]The whole blood samples of 67 Wuding chickens were collected from the wing veins,and the genomic DNA was extracted.The PCR products digested with Hsp92Ⅱrestriction enzyme were analyzed via PCR-RFLP assay.Result]Three genotypes of AA,AB and BB were observed at the synonymous mutation site of c.A99G,in which the frequency of A gene was 0.6866 and that of B gene was 0.3134;the PIC was 0.3378,indicating that the site was a moderate polymorphic site;χ2 fitness test showed that the adiponectin gene c.A99G site was in equilibrium.Conclusion]The PCR-RFLP method effectively detected the c.A99G mutation of adiponectin gene of Wuding chicken.There were 3 genotypes at this locus,and the alleles were at the level of moderate genetic polymorphism and were in equilibrium. |
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| Keywords: | Wuding chicken adiponectin gene PCR-RFLP |
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