| 植物乳杆菌(Lactobacillus plantarum)8-6产细菌素发酵条件的优化 |
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| 引用本文： | 李亚,谈重芳,王雁萍,李宗伟.植物乳杆菌(Lactobacillus plantarum)8-6产细菌素发酵条件的优化[J].草地学报,2012(2):368-372,377. |
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| 作者姓名： | 李亚 谈重芳 王雁萍 李宗伟 |
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| 作者单位： | 郑州大学河南省离子束生物工程重点实验室 |
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| 基金项目： | 河南省自然研究计划(2010B180029) |
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| 摘 要： | 对植物乳杆菌(Lactobacillus plantarum)8-6产细菌素的发酵条件进行了优化,分别研究了培养时间、温度、接种量、培养基起始pH值、培养基碳源、氮源等因素对细菌素产生的影响,通过单因素水平试验和正交试验,确定产细菌素的最佳培养基组合和最佳发酵条件为葡萄糖3%,胰蛋白胨2%,蛋白胨1%,酵母膏1%,硫酸镁0.058%,吐温-80 0.2%,30℃培养24h,培养基起始pH值为6.5,接种量2%。乳杆菌8-6优化后效价为1825.56IU/mL,比优化前提高了373.15%。
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| 关 键 词： | 植物乳杆菌 细菌素 发酵条件 |
Optimization of bacteriocin production by Lactobacillus plantarum 8-6 |
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| LI Ya,TAN Zhongfang,WANG Yanping,LI Zongwei.Optimization of bacteriocin production by Lactobacillus plantarum 8-6[J].Acta Agrestia Sinica,2012(2):368-372,377. |
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| Authors: | LI Ya TAN Zhongfang WANG Yanping LI Zongwei |
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| Institution: | (Provincial Key Laboratory of Ion Beam Bio-engineering,Zhengzhou University,Zhengzhou 450052,China) |
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| Abstract: | Fermentation conditions of bacteriocin produced by Lactobacillus plantarum 8-6 were optimized.Effects of culture time,temperature,inoculum,initial pH value,carbon source and nitrogen on bacteriocin production were studied.The optimum media composition and fermentation conditions were obtained by single-factor and orthogonal experiments as follows: glucose 3%,tryptone 1.5%,peptone 1.5%,yeast extract 1%,magnesium sulfate 0.058%,tween-80 0.2%,culture temperature 30℃,culture time 28h,initial pH value 6.5 and inoculum 2%.Under the above conditions,the production of bacteriocin was 1825.56 IU/ml,which increased by 373.15% compared to that before optimization. |
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| Keywords: | Lactobacillus plantarum bacteriocin fermentation conditions |
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