| 猪PLSCR4基因外显子区PCR-RFLP遗传多态性分析 |
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| 引用本文： | 陈洁,白莹,徐芹芹,刘玉芳,方美英.猪PLSCR4基因外显子区PCR-RFLP遗传多态性分析[J].中国畜牧杂志,2013(11):1-4. |
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| 作者姓名： | 陈洁 白莹 徐芹芹 刘玉芳 方美英 |
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| 作者单位： | 中国农业大学动物科技学院 |
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| 基金项目： | 自然科学基金资助项目(31072002);教育部“新世纪优秀人才资助项目”(NCET-11-0480) |
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| 摘 要： | 本研究利用PCR-RFLP技术对我国6个地方猪种、中国野猪以及杜洛克、约克夏和长白3个国外猪品种,共307头猪的磷脂爬行酶基因(PLSCRs)外显子区的遗传变异进行了研究。结果表明:PLSCR4第7外显子处存在T68C的同义突变,第8外显子处存在G4A的错义突变;在T68C突变位点处,杜洛克、约克夏、长白猪、二花脸、五指山和民猪6个猪群优势等位基因为T,荣昌和野猪群体中优势等位基因为C;在G4A突变位点处,杜洛克、约克夏、长白猪、二花脸、五指山和民猪群体中优势等位基因为G,藏猪、荣昌、金华及野猪群体中优势等位基因A。
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| 关 键 词： | PLSCR4基因 PCR-RFLP 遗传多样性 猪 |
Genetic Diversity of Exon Regions of Phospholipid Scramblase Gene among Different Pig Breeds |
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| CHEN Jie,BAI Ying,XU Qin-qin,LIU Yu-fang,FANG Mei-ying.Genetic Diversity of Exon Regions of Phospholipid Scramblase Gene among Different Pig Breeds[J].Chinese Journal of Animal Science,2013(11):1-4. |
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| Authors: | CHEN Jie BAI Ying XU Qin-qin LIU Yu-fang FANG Mei-ying |
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| Institution: | *(College of Animal Science & Technology,China Agricultural University,Beijing 100193,China) |
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| Abstract: | The exons of PLSCR4 gene were analyzed in 307 pigs including six Chinese native pig breeds,Chinese wild boar and three exotic pig breeds(Duroc,Yorkshire and Landrace) by means of PCR-RFLP technology.The results showed that the T68C transition mutation within exon 7 of PLSCR4 was a synonymous mutation and the G4A transition mutation within exon 8 was a missense mutation;The genetic variations were found among all the pig samples at the T68C site of the exon 7,T was the dominant allele in Duroc,Yorkshire,Landrace,Erhualian,Wuzhishan and Min and C was the dominant allele in Rongchang and Chinese wild boar.At the G4A site of exon 8,G was dominant allele in Duroc,Yorkshire,Landrace,Erhualian,Wuzhishanw and Min and A was dominant allele in tibet pig,Rongchang,Jinhua and Chinese wild boar. |
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| Keywords: | PLSCR4 gene PCR-RFLP genetic diversity pig |
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