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1.
响应面优化酶法提取马铃薯皮渣中多酚的工艺   总被引:1,自引:0,他引:1  
为研究酶法提取马铃薯皮渣多酚的最优工艺,采用单因素试验考察复合酶添加量、酶解时间、酶解pH及浸提剂乙醇浓度对多酚提取量的影响,并运用中心组合设计及响应面优化马铃薯皮渣多酚的最佳工艺参数。结果表明,各提取因素对马铃薯皮渣多酚提取量影响的次序为:浸提剂乙醇浓度酶解pH酶添加量。当复合酶添加量1.4%,酶解时间90 min,酶解pH 6.10,浸提剂乙醇浓度47%时,马铃薯皮渣多酚的提取量可达3.21 mg/g。  相似文献   

2.
以蒲公英、雪梨为主要原料,研制蒲公英雪梨复合保健饮料,并探讨各因素对蒲公英浸提、雪梨汁酶解及饮料调配效果的影响。结果表明,蒲公英的最佳超声浸提条件为超声频率40 kHz,料液比1∶25,提取温度70℃,提取次数2次,提取时间1.5 h;雪梨汁的最佳酶解工艺为酶解时间2.0 h,酶解温度40℃,复合酶添加量0.6%,pH值3.5;饮料最佳调配工艺为雪梨汁添加量20%,冰糖添加量8%,苹果酸添加量0.15%,蒲公英浸提液添加量30%,蜂蜜添加量0.005%,制得的产品风味独特、营养丰富、质量最佳。  相似文献   

3.
对海带加工下脚料中多糖的酶解提取工艺和化学稳定性进行研究。在单因素试验基础上,采用响应面法对酶解辅助提取的工艺参数进行优化,得出4种酶解因素对海带加工下脚料中多糖提取量影响顺序依次为:复合酶添加量>pH >酶解时间>温度。最优工艺条件为:液料比40∶1(mL/g),酶解时间135 min,酶解温度55 ℃,酶解液pH 6.0,复合酶添加量2.0%。在该条件下,制得的海带多糖提取量为149.662 g/kg。化学稳定性试验表明,海带多糖提取物在高温和酸性环境下,具有良好的化学稳定性,对碱性环境稳定性较差,是一种化学稳定性较好的天然活性多糖。  相似文献   

4.
利用超声法辅助提取光皮木瓜渣中的多酚,考察了乙醇浓度、料液比、超声时间、提取温度等因素对提取量的影响。通过单因素试验和正交试验确定了光皮木瓜渣多酚提取的适宜工艺条件为:乙醇体积分数55%,料液比1∶17,超声时间40 min,提取温度60℃,在此优化条件下,光皮木瓜渣多酚提取量为9.73%;该类光皮木瓜渣多酚粗提液具有较强的抗氧化活性,是一类具有开发价值的天然抗氧化剂。  相似文献   

5.
通过单因素试验,分析了不同浸提剂、浸提剂浓度、料液比、浸提时间、浸提温度5个因素对桑籽皮多酚提取量的影响,通过正交试验,确定了桑籽皮多酚的最佳提取条件为:浸提剂(丙酮)体积分数为70%,料液比1∶35,回流温度45℃,回流时间1.5h。在此提取条件下,提取量达到1.332mg/g干籽皮,为桑籽皮多酚的工业化提取提供科学依据。  相似文献   

6.
以石榴渣为原料,研究了提取溶剂及其浓度、浸提温度、浸提时间和料液比对多酚提取效果的影响,并通过正交试验对4因素影响下的提取工艺进行了优化,确定了石榴渣多酚乙醇提取的最优工艺为:乙醇体积分数50%,浸提温度60℃,料液比1:20,浸提时间4h,以此优化工艺进行试验,一次提取得率为0.51%,二次提取得率为0.69%。超声波辅助提取的最佳处理时间为30min,提取2次,多酚得率为0.79%。原料干燥方式采用烘箱干燥较好。该工艺具有一定的实用性和可行性。  相似文献   

7.
以人参总皂苷和多糖提取得率为考察指标,采用酶解组合高速匀浆法同时提取人参中的两种重要成分,比较不同酶及组合对两者得率的影响,筛选出提取效果较好的果胶酶。通过单因素试验对酶解温度、酶解时间、酶解pH、酶添加量和人参粉末粒径分别进行优化,继而进行响应面试验,得到最佳酶解工艺为:酶解温度60℃,酶解pH 4.7,酶解时间4 h,酶添加量6%,人参粉末粒径约285μm,此时人参总皂苷得率为6.88%,多糖得率为28.58%。将提取后的渣料进行高速匀浆提取,以乙醇浓度、匀浆时间、固液比为变量设计正交试验,得到最佳提取条件为:乙醇浓度0%(蒸馏水),匀浆时间4 min,固液比1∶50(g/mL),此条件下人参总皂苷得率为2.56%,多糖得率为16.92%。两步提取法人参总皂苷得率9.44%,多糖得率45.50%,均高于现有方法的提取得率。  相似文献   

8.
为获得超声波辅助复合酶提取苹果多酚的最佳工艺,在单因素试验的基础上,采用正交设计,以多酚得率为评价指标,对工艺条件进行优化。结果表明,最适合苹果多酚提取的复合酶为纤维素酶和果胶酶(质量比1∶1),添加量0.07%,酶解时间60 min,提取温度65℃,超声时间10 min,在此工艺条件下多酚得率为1.99 mg·g~(-1)。  相似文献   

9.
欧李叶片中多酚的提取工艺优化   总被引:1,自引:1,他引:0  
【研究目的】以欧李叶片为试验材料,多酚提取率为评价指标,探讨欧李叶片多酚提取的工艺条件。【方法】以丙酮为提取剂,选定浸提时间、溶剂浓度、料液比、浸提温度为考察的4个因素,以L9(34)正交试验设计,采用常规提取法和微波提取法研究提取欧李叶片中多酚的最佳条件。【结果】利用常规法从欧李叶片中提取多酚各因素对多酚提取率影响的主次顺序为:浸提温度>浸提时间>固液比>提取剂浓度,得出最优方案为A1B3C3D3,与试验中最佳处理组合A1B3C3D3相一致;利用微波法从欧李叶片中提取多酚各因素对提取率影响的主次顺序为:提取剂浓度>固液比>辐射时间>辐射功率,得出最优方案为A1B2C3D2,与试验中最佳处理组合A1B2C2D2不一致。【结论】欧李叶片中多酚提取的最佳工艺为70%丙酮溶液,固液比1︰450,浸提时间1.5 h,浸提温度70℃。  相似文献   

10.
以干菠萝皮渣为原料,采用木瓜蛋白酶法提取菠萝皮渣中的多糖,通过单因素试验和响应曲面试验,确定最优的提取工艺为木瓜蛋白酶添加量1.29%,料液比1∶16(g∶mL),酶解pH值6.37,酶解时间3.24 h,多糖提取率达到4.89%;所提多糖具有清除羟自由基的能力。  相似文献   

11.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans.  相似文献   

12.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes.  相似文献   

13.
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol...  相似文献   

14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed.  相似文献   

15.
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses.  相似文献   

16.
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination...  相似文献   

17.
Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums.  相似文献   

18.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf 1) and recessive for the other (Rf 2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph 3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf 1(one of two dominant additive genes controlling early season leafing out) indicated that Rf 1and Rf 2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf 1, and 0.15 for Lf 1-Sph 3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph 3.It is suggested that competitive disadvantage of lf 1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph 3. Poor performance of lf 1- (and possibly lf 2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off).  相似文献   

19.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho...  相似文献   

20.
T. Visser  E. H. Oost 《Euphytica》1981,30(1):65-70
Summary Apple and pear pollen was irradiated with doses of 0, 50, 100, 250 and 500 krad (gamma rays) and stored at 4°C and 0–10% r.h. From the in-vitro germination percentages an average LD 50 dose of about 220 krad was estimated. For both irradiated and untreated pollen a close and corresponding lineair relationship existed between germination percentage and pollen tube growth.Irradiated pollen was much more sensitive to dry storage conditions than untreated pollen, resulting in less germination and more bursting. Apparently, irradiation caused the pollen cell membrane to lose its flexibility faster than normal. Rehydration of dry-stored, irradiated pollen in water-saturated air restored germination percentages up to their initial levels. The importance of this procedure in germination trials is stressed.  相似文献   

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