共查询到20条相似文献，搜索用时 859 毫秒
1.
M.C. Marinho M.A. Pinho R.D. Mascarenhas F.C. Silva M.M. Lordelo L.F. Cunha J.P.B. Freire 《Livestock Science》2007,108(1-3):240-243
Forty eight 21 days old piglets were used to compare the effect of prebiotic or probiotic supplementation and ileo rectal anastomosis on the morphology of the small intestine. Half of the piglets were maintained intact and the other half was subjected to an ileo rectal anastomosis (IRA). Each group of piglets received one of the following diets: 1) basal diet (C), 2) basal diet supplemented with a Xylo-oligosaccharide (XOS), 3) basal diet supplemented with a Saccharomyces cerevisiae (SC) and 4) basal diet supplemented with XOS and SC. Villus height was greatest with XOS and with XOS + SC, only in the ileum, as compared to controls. In the duodenum, crypt width was highest in the control group, but no significant differences were found in the jejunum and ileum. The IRA piglets had longer villi in the jejunum and shorter villi in the ileum. The crypt depth was greater in the duodenum and in the ileum of IRA piglets. Villus height/crypt depth was lower in the duodenum and in the ileum, in the IRA piglets. In conclusion, the XOS, but not the SC, moderately modified the intestinal morphology. The IRA modified the intestinal villus and crypt architecture but its consequence on the absorption of nutrients needs to be investigated. 相似文献
2.
本试验旨在研究水牛初乳粉、常乳粉对0～72 h新生仔猪小肠组织形态结构发育的影响。试验以新生仔猪为研究对象,分别饲喂水牛初乳粉（SCR组）、水牛常乳粉（SC组）、葡萄糖盐水（PY组）,以0日龄新生仔猪（XD组）作为对照。结果表明,水牛初乳粉使新生仔猪小肠绒毛密集、粗壮、排列整齐,肌层厚度、固有膜厚度增加。SCR组与XD组相比,十二指肠绒毛高度、绒毛宽度、固有膜厚度和空肠绒毛宽度、肌层厚度及回肠绒毛高度均极显著增加（P＜0.01）;SCR组与PY组相比,十二指肠绒毛宽度及空肠绒毛宽度、肌层厚度均极显著增加（P＜0.01）。水牛常乳粉使新生仔猪小肠黏膜受损,黏膜上皮脱落,肌层变薄,固有膜裸露,小肠绒毛坍塌、稀疏变短。SC组与XD组相比,十二指肠肌层厚度和空肠绒毛高度、绒毛宽度、肌层厚度、固有膜厚度及回肠绒毛宽度、固有膜厚度均呈降低趋势,但差异均不显著（P＞0.05）;SC组与PY组相比,空肠肌层厚度显著降低（P＜0.05）。综上所述,水牛初乳粉能显著促进新生仔猪小肠发育,尤其对空肠影响最大,其次为十二指肠,而对回肠的影响相对较小;水牛常乳粉不能对新生仔猪肠黏膜形成保护并促进其生长发育,在新生仔猪最初发育的72 h内其价值不如葡萄糖盐水。 相似文献
3.
应用组织切片技术对11～53日龄吉林白鹅小肠绒毛高度、绒毛宽度、隐窝深度和肌层厚度进行测定。结果显示,小肠各段绒毛高度及宽度随日龄增加整体上呈增长趋势,空肠绒毛最长,回肠绒毛高度、宽度在39日龄达到峰值,早于空肠和十二指肠。回肠隐窝深度11～53日龄随日龄增加而增加,十二指肠和空肠隐窝深度在39日龄达到峰值,39～53日龄变浅。小肠各段肌层厚度与日龄呈正相关,回肠的肌层厚度在46日龄达到高峰,十二指肠和空肠的肌层厚度在53日龄达到高峰。小肠各段绒毛高度/隐窝深度的比值随日龄增加而增加,53日龄时空肠的绒毛高度/隐窝深度的比值最高。 相似文献
4.
Sehm J Lindermayer H Dummer C Treutter D Pfaffl MW 《Journal of animal physiology and animal nutrition》2007,91(7-8):289-296
Alternative food ingredients, e.g. secondary plant compounds, are discussed to have beneficial effects and improve gut health. In this study, the effect of three different diets - normal piglets starter without additives, with apple pomace or with red-wine pomace - on the intestinal morphology was investigated from 3 days prior to weaning to 4 weeks post-weaning. At five time points, six piglets from each treatment group were slaughtered; at first time point only six animals from control group were slaughtered. Villus height, crypt depth and breadth of villi and crypts were determined in the jejunum, ileum and colon in 78 piglets. Additionally, the area of the Peyer's patches in the ileum was measured. In jejunum (p < 0.01) and ileum (p < 0.001) the villus length in the control group decreased after weaning but increased over the entire feeding experiment (p < 0.001). In the two-pomace groups, no decrease was measured after weaning. In jejunum, an increase in villi breadth occurred, 73% in the control group and approximately 10% in both treatment groups. A 35% increase was found in the ileum in all groups. Peyer's patches area increased approximately 21% in the control group over 26 days of treatment, whereas in other groups no significant differences were found. Different polyphenol rich pomaces have diverse effects in the gastrointestinal tract. Red-wine pomace has an inhibitory effect on the jejunum villi growth, whereas apple and red-wine pomace have stimulating effect on crypt size in piglet colon. Apple and red-wine pomace can reduce the GALT activation via the Peyer's patches in the ileum. In conclusion, the flavanoids rich feeding regimen showed positive effects on villi morphology, GALT activation and can improve pig health. 相似文献
5.
本试验旨在研究豆粕中大分子蛋白质和肽含量对仔猪小肠绒毛结构的影响。采用凝胶过滤色谱技术分析豆粕中大分子蛋白质和肽含量。选取40头断奶仔猪,随机分成4个处理:去皮豆粕处理、发酵豆粕C处理、发酵豆粕E处理和动物蛋白处理。试验期5周。结果表明:去皮豆粕中大分子蛋白质含量约占80%,发酵豆粕中约占50%,甚至降至24%;发酵豆粕中肽含量约占19%,去皮豆粕中仅有2.7%。与去皮豆粕处理相比,发酵豆粕C处理十二指肠绒毛高度显著提高(P0.05),发酵豆粕E、发酵豆粕C处理十二指肠、空肠隐窝深度显著下降(P0.05),发酵豆粕E、发酵豆粕C处理十二指肠和空肠的绒毛高度/隐窝深度均显著升高(P0.05)。由此可见,豆粕中大分子蛋白质含量与小肠绒毛高度/隐窝深度呈负相关;发酵豆粕能够在断奶仔猪饲料中使用,并且有效改善断奶仔猪小肠绒毛结构。 相似文献
6.
7.
This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa) on intestinal absorption and barrier function, intestinal morphology and antioxidant capacity of 7 days old piglets. Twenty-four 7 days old piglets were allotted to four treatments:control group (artificial milk), STa group (artificial milk +2×109 CFU E.coli LMG194-pBAD-STa), LMG194 group (artificial milk +2×109 CFU E.coli LMG194), and K88 group (artificial milk +2×109 CFU E.coli K88).The pigs were treated with E.coli on the 5th day and slaughtered on the 7th day. The results showed that, compared with the control group, villus height in jejunum, ileum and duodenum, crypt depth and villus height/crypt depth in duodenum, and small intestine villi surface area were significantly decreased in STa group (P<0.05). The activities of catalase (CAT) in ileum and colon, total nitric oxide synthase (TNOS) in serum, ileum, jejunum and colon, inducible nitric oxide synthase (iNOS) in colon were significantly decreased in STa group (P<0.05). STa group also had a higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in serum (P<0.05). These results suggested that recombinant E.coli expressing STa could lead to intestinal injury and oxidative stress of 7 days old piglets. 相似文献
8.
试验旨在研究表达耐热肠毒素（STa）的重组大肠杆菌对7日龄仔猪肠道形态结构及抗氧化功能的影响。选取24头7日龄仔猪,随机分在4个日粮处理组,分别为对照组（人工乳）,STa组（人工乳+2×109 CFU重组菌LMG194-pBAD-STa）,LMG194组（人工乳+2×109 CFU大肠杆菌LMG194）,K88组（人工乳+2×109 CFU大肠杆菌K88）。试验第5天进行攻毒,第7天屠宰取样,测量小肠黏膜的绒毛高度和隐窝深度,检测空肠、回肠、结肠及血清中的过氧化氢酶（CAT）、总一氧化氮合成酶（TNOS）与诱导型一氧化氮合酶（iNOS）的活力及血清中丙二醛（MDA）与过氧化氢（H2O2）的含量。试验结果表明,与对照组相比,STa组仔猪各肠段绒毛高度均显著降低（P<0.05）,十二指肠隐窝深度及绒毛高度与隐窝深度的比值显著降低（P<0.05）,十二指肠、空肠、回肠绒毛表面积显著降低（P<0.05）;同时,STa组回肠、结肠CAT活力显著降低,血清、空肠、回肠和结肠中的TNOS活力显著降低（P<0.05）,STa组结肠iNOS活力显著降低（P<0.05）,STa组血清中的MDA与H2O2含量显著提高（P<0.05）。结果显示,表达STa的重组大肠杆菌可导致7日龄仔猪肠道结构损伤和抗氧化能力下降。 相似文献
9.
本试验旨在研究丁酸钠对断奶羔羊胃肠道发育的影响。选取24只42日龄的断奶羔羊,随机分成4组,每组6个重复。空白对照组饲喂基础饲粮,抗生素组饲喂在基础饲粮中添加250 mg/kg黄霉素的饲粮,丁酸钠A组和丁酸钠B组分别饲喂在基础饲粮中添加2和3 g/kg包膜丁酸钠的饲粮。各组分别在试验第14天、第28天选取3只羔羊屠宰取样。结果表明:试验第14天时,各组断奶羔羊的瘤胃相对质量和相对容积以及各胃肠道形态指标均无显著差异(P>0.05);试验第28天时,与空白对照组相比,丁酸钠B组瘤胃背囊的肌层厚度,瘤胃腹囊的肌层厚度、乳头高度,十二指肠、空肠和回肠绒毛高度,空肠绒毛表面积以及空肠和回肠绒毛高度/隐窝深度显著增加(P<0.05)。由此可见,在饲粮中添加3 g/kg包膜丁酸钠并持续饲喂28 d可以促进断奶羔羊瘤胃乳头和肌层以及肠道绒毛的生长,增加肠道绒毛高度与隐窝深度的比值,有利于胃肠道的发育。 相似文献
10.
试验旨在建立猪流行性腹泻病毒（PEDV）感染幼龄仔猪肠道损伤模型。试验选用16头7日龄健康幼龄仔猪（杜×长×大）,随机分为两个处理组：对照组和PEDV组,每个处理8个重复,每个重复1头猪。试验期为10 d,试验期间两个处理组饲喂相同的基础日粮。于试验第7天晚上对PEDV组仔猪口腔灌服PEDV病毒（剂量为104.5 TCID50）,对照组灌服等量的生理盐水。于试验第10天早上空腹灌服D-木糖（0.1 g/kg体重）,1 h后前腔静脉采血,屠宰取样,取十二指肠、空肠、回肠等组织样品,测定平均日增重（ADG）、腹泻率（DR）、肠道形态结构、肠道黏膜损伤相关基因mRNA水平。试验结果表明：①PEDV感染显著降低了仔猪ADG（P<0.05）,极显著提高了仔猪腹泻率（P<0.01）;②PEDV感染极显著降低了血浆D-木糖含量、空肠和回肠绒毛高度、十二指肠、空肠和回肠绒毛高度与隐窝深度的比值（P<0.01）,显著提高了十二指肠和空肠隐窝深度（P<0.05）;③PEDV感染极显著提高了仔猪空肠黏膜PEDV M基因的相对表达量（P<0.01）,极显著降低了肠绒毛蛋白（villin）和肠型脂肪酸结合蛋白（i-FABP）基因的相对表达量（P<0.01）。以上结果表明,口腔灌服PEDV可以成功诱导建立幼龄仔猪肠道损伤模型。 相似文献
11.
【目的】 通过研究槲皮素对猪流行性腹泻病毒（PEDV）感染仔猪肠道形态、肠道抗氧化功能及空肠脂质代谢相关基因相对表达量的影响,旨在探讨槲皮素对PEDV感染仔猪肠道的保护作用。【方法】 选取18头健康的7日龄断奶仔猪,随机分为3组：对照组、PEDV组和槲皮素＋PEDV组,每组6个重复,每个重复1头猪。试验期8 d,于试验第0~7天,对槲皮素＋PEDV组仔猪每天口腔灌服10 mg/kg BW的槲皮素,其余组灌服等体积的人工奶。于试验第5天晚上给PEDV组和槲皮素＋PEDV组仔猪口腔灌服104.5TCID50的PEDV,对照组仔猪灌服相同体积的PBS溶液。于试验第8天早上屠宰,取仔猪十二指肠、空肠、回肠及结肠组织样品进行检测。【结果】 与对照组相比,PEDV组仔猪空肠和回肠的绒毛高度以及绒毛高度与隐窝深度比值极显著降低（P<0.01）,空肠、回肠和结肠中谷胱甘肽过氧化物酶（GSH-Px）和总超氧化物歧化酶（T-SOD）的活力显著降低（P<0.05）,十二指肠、空肠、回肠和结肠中过氧化氢酶（CAT）的活力显著降低（P<0.05）,回肠和结肠中丙二醛（MDA）的含量显著升高（P<0.05）,空肠中载脂蛋白A1（APOA1）、载脂蛋白B （APOB）、载脂蛋白C2（APOC2）、脂肪酸结合蛋白2（FABP2）、酰基辅酶A合成酶长链家族成员3（ACSL-3）和脂肪酸合酶（FASN）基因的相对表达量极显著下调（P<0.01）。与PEDV组相比,槲皮素＋PEDV组仔猪空肠和回肠的绒毛高度以及绒毛高度与隐窝深度比值极显著提高（P<0.01）,空肠、回肠和结肠中GSH-Px和T-SOD的活力显著提高（P<0.05）,十二指肠、空肠和结肠中CAT的活力显著提高（P<0.05）,回肠和结肠中MDA的含量显著降低（P<0.05）,空肠中APOB、FABP2和FASN基因的相对表达量极显著上调（P<0.01）。【结论】 添加槲皮素可有效缓解PEDV感染导致的仔猪肠道损伤,提高仔猪肠道抗氧化能力以及空肠脂质代谢的能力。 相似文献
12.
An experiment was conducted to evaluate the effects of dietary spray-dried bovine and porcine plasma and spray-dried bovine colostrum on growth performance and intestinal histology in weaner pigs. Thirty-two 21-day-old piglets (6.65 ± 0.14 kg) were allocated to receive one of four dietary treatments: control, bovine plasma, porcine plasma, and bovine colostrum at weaning and another 8 piglets were killed at weaning to provide baseline data. The experimental diets were offered ad libitum for one week, after which animals were killed and post-mortem measurements obtained. No differences in average daily feed intake and growth rate were observed among dietary treatment groups (P > 0.05). Baseline piglets had taller villi and shallower crypts (P < 0.05) in the proximal jejunum, mid jejunum and distal ileum than those observed a week after weaning, irrespective of dietary treatment. Weaning-related expansion of intestinal lamina propria CD4+ and CD8+ T lymphocyte populations was observed (P < 0.05). Complex and variable treatment effects on villus height, crypt depth, villus and crypt goblet cell density, and lamina propria T cell density were observed, suggesting that the tested protein sources do not share a common or simple mode of action. 相似文献
13.
太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构及盲肠内容物菌群的影响 总被引：1，自引：0，他引：1
本试验旨在研究饲粮中添加太子参茎叶多糖对断奶仔猪肠道免疫功能、肠黏膜形态结构和盲肠内容物菌群的影响。选取25日龄断奶的"大白×长白"二元杂交仔猪120头,按体重相近原则随机分成4个组,每组3个重复,每个重复10头猪。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加500(0.05%多糖组)、1 000(0.10%多糖组)和1 500 mg/kg(0.15%多糖组)太子参茎叶多糖。试验期30 d。结果表明:与对照组相比,各试验组十二指肠白细胞介素-4(IL-4)含量均显著升高(P0.05),0.10%多糖组十二指肠干扰素-γ(IFN-γ)含量显著升高(P0.05),0.10%和0.15%多糖组回肠分泌型免疫球蛋白A(SIg A)含量均显著升高(P0.05);0.10%和0.15%多糖组十二指肠和空肠绒毛高度显著或极显著增加(P0.05或P0.01),十二指肠绒毛高度/隐窝深度(V/C)值显著增加(P0.05);0.15%多糖组回肠绒毛高度显著增加(P0.05);各试验组盲肠内容物中的大肠杆菌数量显著降低(P0.05),乳酸杆菌数量显著增加(P0.05)。由此可见,饲粮中添加太子参茎叶多糖能够提高断奶仔猪肠道免疫功能,增加小肠绒毛高度和V/C值,调节盲肠内容物菌群结构,维持肠道微生态平衡;其适宜添加量为0.10%。 相似文献
14.
15.
益生菌制剂对早期断奶仔猪肠道pH、黏膜形态结构和挥发性脂肪酸含量的影响 总被引：3，自引：0，他引：3
本试验旨在研究益生菌制剂对早期断奶仔猪肠道pH、黏膜形态结构和挥发性脂肪酸含量的影响.选用48头平均体重为(6.39±0.42)kg的(21±2)日龄“杜×长×大”三元杂交断奶仔猪,按性别、体重和窝源基本一致原则随机分为2个组,每个组4个重复,每个重复6头猪.试验1组为对照组,采用基础饲粮;试验2组为益生菌组,每千克基础饲粮中添加1×109 CFU活菌.试验期为21d.结果表明:1)28日龄时,益生菌组回肠内容物pH显著低于对照组(P＜0.05).2)和对照组相比,添加益生菌制剂,28日龄时,显著增加空肠、回肠绒毛高度和上皮内淋巴细胞数量(P＜0.05),显著提高回肠杯状细胞数量(P＜0.05),显著降低回肠隐窝深度(P＜0.05);35日龄时,显著增加空肠、回肠绒毛高度(P＜0.05),显著提高空肠上皮内淋巴细胞数量(P＜0.05),显著降低空肠、回肠隐窝深度(P＜0.05);42日龄时,显著增加空肠、回肠绒毛高度,显著提高空肠上皮内淋巴细胞和杯状细胞数量(P＜0.05),显著降低空肠隐窝深度(P＜0.05).3)28日龄时,益生菌组盲肠内容物中丁酸和乳酸含量显著高于对照组(P＜0.05);35日龄和42日龄时,益生菌组盲肠内容物中乙酸、丙酸、丁酸和乳酸含量显著高于对照组(P＜0.05).由结果可知,添加益生菌制剂能改善断奶应激对绒毛高度和隐窝深度的影响,提高肠道淋巴细胞数量和挥发性脂肪酸含量,降低肠道pH,从而缓解断奶应激对肠道环境的影响. 相似文献
16.
试验旨在研究植物小肽对断奶仔猪生长性能、腹泻率及肠道发育的影响。选择体重为9.56 kg左右的35日龄健康杜长大三元杂种断奶仔猪120头,按照体重相似的原则随机分为2个处理,每个处理设6个重复,每个重复10头仔猪。对照组饲喂基础饲粮(含2.5%鱼粉),试验组添加2%植物小肽(替代基础饲粮中2.5%鱼粉)。试验期28 d。结果表明,植物小肽组仔猪日增重和日采食量分别比对照组提高5.88%(P<0.05)和2.67%(P<0.05),料重比显著降低2.74%(P<0.05)。与对照组相比,植物小肽组仔猪十二指肠和空肠的绒毛高度显著提高(P<0.05),十二指肠和空肠的隐窝深度显著降低(P<0.05),十二指肠和空肠的绒腺比(V/C)也显著提高(P<0.05)。综合来看,断奶仔猪饲粮中2%植物小肽替代2.5%鱼粉促进了仔猪肠道发育,提高了生长性能。 相似文献
17.
LIU Xiao-long FAN Huan CHEN long-bin LI Hai-hua QIAO Jia-yun WANG Wen-jie 《中国畜牧兽医》2017,44(11):3187-3194
In order to investigate the effects of cecropin and synbiotics on the intestinal mucosal morphology and intestinal mucosal immune cells of AA broilers. 480 of 1-day-old healthy AA broilers were chosen and randomly divided into 4 groups with 4 replicates per group and 30 broilers per replicate. The broilers in group Ⅰ(control group) were fed basal diet,while that in groups Ⅱ to Ⅳ were fed the basal diet supplemented with 0.5% cecropin,0.3% synbiotics,0.5% cecropin+0.3% synbiotics,respectively. The trial lasted for 42 days. The result showed that compared with group Ⅰ,the villus height of duodenum and ileum in group Ⅳ were extremely significantly increased (P<0.01).The crypt depth of jejunum and ileum in group Ⅱ,and that of small intestine in group Ⅲ were significantly or extremely significantly decreased (P<0.05;P<0.01).The villus height/crypt depth of small intestine in groups Ⅱ and Ⅲ,and that of duodenum and ileum in group Ⅳ were significantly or extremely significantly increased (P<0.05;P<0.01).The villus width of jejunum in group Ⅱ was significantly decreased (P<0.05),while the villus width of duodenum and jejunum in group Ⅲ,that of jejunum and ileum in group Ⅳ were extremely significantly decreased (P<0.01).The mucous thickness of jejunum in groups Ⅱ and Ⅲ were significantly increased (P<0.05),while that of small intestine in group Ⅳ was extremely significantly increased (P<0.01).The number of the intraepithelial lymphocyte of ileum in group Ⅱ was significantly increased (P<0.05),and that of duodenum in group Ⅲ and small intestine in group Ⅳ were extremely significantly increased (P<0.01).The number of the goblet cell of duodenum in group Ⅲ,duodenum and ileum in group Ⅳ were extremely significantly increased (P<0.01).The mast cell of jejunum in group Ⅳ was significantly increased (P<0.05).In conclusion,both the cecropin and synbiotics could improve the small intestine mucosal structure and promote the small intestine mucosal immunocompetent cells proliferation in AA broiler, and the effect of combined utilization would be better. 相似文献
18.
为研究日粮中添加天蚕素、合生素对AA肉鸡小肠黏膜形态及免疫细胞的影响,试验选用480只1日龄健康AA肉鸡,随机分为4组,每组4个重复,每个重复30只鸡。对照组（Ⅰ组）饲喂基础日粮,试验组（Ⅱ~Ⅳ组）分别在基础日粮中添加0.5%天蚕素、0.3%合生素、0.5%天蚕素+0.3%合生素。试验期42 d。结果显示,与Ⅰ组相比,Ⅳ组十二指肠、回肠绒毛高度极显著升高（P<0.01）;Ⅱ组空肠和回肠、Ⅲ组小肠各段隐窝深度显著或极显著降低（P<0.05;P<0.01）;Ⅱ、Ⅲ组小肠各段和Ⅳ组十二指肠、回肠绒毛高度/隐窝深度显著或极显著升高（P<0.05;P<0.01）;Ⅱ组空肠绒毛宽度显著降低（P<0.05）,Ⅲ组十二指肠和空肠、Ⅳ组空肠和回肠绒毛宽度均极显著降低（P<0.01）;Ⅱ、Ⅲ组空肠黏膜厚度显著升高（P<0.05）,Ⅳ组小肠各段黏膜厚度均极显著升高（P<0.01）。Ⅱ组回肠上皮内淋巴细胞数量显著升高（P<0.05）,Ⅲ组十二指肠、Ⅳ组小肠各段上皮内淋巴细胞数量极显著升高（P<0.01）;Ⅲ组十二指肠、Ⅳ组十二指肠和回肠杯状细胞数量极显著升高（P<0.01）;Ⅳ组空肠肥大细胞数量显著升高（P<0.05）。综上所述,天蚕素、合生素单独或联合添加均能改善AA肉鸡小肠黏膜结构,促进小肠黏膜免疫细胞增殖,联合添加效果最佳。 相似文献
19.
α-酮戊二酸对LPS慢性应激仔猪小肠黏膜形态与功能的影响 总被引：5，自引：3，他引：2
为了探讨α-酮戊二酸(AKG)能否缓解LPS慢性应激导致的仔猪小肠黏膜损伤及其机理,本试验研究了AKG对LPS慢性应激仔猪的小肠黏膜形态、血浆D-木糖的含量、血浆和小肠黏膜二胺氧化酶(DAO)活性及小肠黏膜mTOR及磷酸化的mTOR表达量的影响.18头(24±1)日龄健康断奶仔猪随机分成3个处理组(空白对照组、应激对照组、AKG组),每个处理6个重复.各组基础日粮一致,空白对照组和应激对照组饲喂基础日粮+1%淀粉,AKG组饲喂基础日粮+1%AKG.试验期为16 d.应激对照组和AKG组仔猪分别于第10、12、14和16天腹膜注射80μg·kg~(-1)BW的LPS,空白对照组注射相应剂量的灭菌生理盐水.第16天注射LPS 2 h后,按0.1g·kg~(-1)BW的剂量给仔猪灌服D-木糖溶液,注射LPS 3 h后,前腔静脉采血.第17天屠宰取小肠组织样,刮取肠黏膜及制作组织切片.结果表明:(1)与空白对照组相比,应激对照组十二指肠、空肠和回肠黏膜绒毛高度/隐窝深度、空肠和回肠磷酸化mTOR/mTOR(P-mTOR/mTOR)显著降低(P<0.05),血浆DAO活性显著升高(P<0.05).(2)与应激对照组相比,AKG组十二指肠、空肠和回肠黏膜绒毛高度/隐窝深度、空肠黏膜DAO活性、血浆D-木糖及十二指肠、空肠和回肠黏膜P-mTOR/mTOR显著升高(P<0.05).结果显示,日粮中添加1%AKG可在一定程度上改善仔猪的小肠组织学形态和吸收功能,缓解LPS慢性应激导致的仔猪小肠黏膜损伤,这与mTOR信号通路有关. 相似文献
20.
玉米-杂粕型日粮添加木聚糖酶对肉鸡免疫功能、肠道形态和微生物菌群的影响 总被引：1，自引：0，他引：1
试验研究在玉米-杂粕型日粮中添加木聚糖酶对肉鸡免疫功能、肠道形态及微生物菌群的影响。试验将480只1日龄艾维因肉公鸡分为4个处理组,分别在基础日粮中添加0、709、1 419、2 128 IU/kg的木聚糖酶,每个处理6个重复,每个重复20只肉鸡,试验期44 d。结果表明:在玉米-杂粕型日粮中添加木聚糖酶对肉鸡免疫器官指数和免疫功能有一定影响1,419 IU/kg组44日龄肉鸡血液E花环和淋巴细胞转化率显著高于对照组(P<0.05);添加木聚糖酶可不同程度提高22日龄肉鸡空肠和回肠肠道肠绒毛长度,降低十二指肠和回肠隐窝深度,显著增加肉鸡肠绒毛长度和隐窝深度的比值(P<0.05);显著降低十二指肠固有层厚度(P<0.05);显著增加44日龄肉鸡乳酸杆菌的数量。结果显示,在玉米-杂粕型日粮中添加1 419 IU/kg水平的木聚糖酶对改善肉鸡免疫指标、肠道形态及微生物菌群效果最佳。 相似文献