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1.
应用组织切片技术对11~53日龄吉林白鹅小肠绒毛高度、绒毛宽度、隐窝深度和肌层厚度进行测定。结果显示,小肠各段绒毛高度及宽度随日龄增加整体上呈增长趋势,空肠绒毛最长,回肠绒毛高度、宽度在39日龄达到峰值,早于空肠和十二指肠。回肠隐窝深度11~53日龄随日龄增加而增加,十二指肠和空肠隐窝深度在39日龄达到峰值,39~53日龄变浅。小肠各段肌层厚度与日龄呈正相关,回肠的肌层厚度在46日龄达到高峰,十二指肠和空肠的肌层厚度在53日龄达到高峰。小肠各段绒毛高度/隐窝深度的比值随日龄增加而增加,53日龄时空肠的绒毛高度/隐窝深度的比值最高。  相似文献   

2.
为了给山羊舍饲提供基础性的组织学理论依据,试验应用普通石蜡切片H.E.染色的方法对60只山羊的小肠进行了组织学研究.结果表明:小肠固有膜厚度舍饲组厚于放牧组,肠绒毛高度舍饲组高于放牧组,隐窝深度舍饲组浅于放牧组,肌层厚度舍饲组薄于放牧组;绒毛高度随舍饲月龄延长而有所降低,固有膜、肌层厚度的变化没有特别的规律性,隐窝深度...  相似文献   

3.
应用普通石蜡切片HE染色的方法对60只山羊的小肠进行了组织学研究,旨在为山羊的舍饲提供基础性的组织学理论依据。结果显示:小肠固有膜厚度舍饲组厚于放牧组;肠绒毛高度舍饲组高于放牧组;隐窝深度舍饲组浅于放牧组;肌层厚度舍饲组薄于放牧组。且绒毛高度随舍饲月龄延长而有所降低,固有膜、肌层厚度的变化没有特别的规律性,隐窝深度随舍饲月龄延长而有所变浅。  相似文献   

4.
断奶日龄对仔猪肠粘膜形态的影响   总被引:33,自引:5,他引:28  
本文研究了不同断奶日龄对仔猪肠粘膜形态的影响。试验分17日龄(1组)、21日龄(2组)、28日龄(3组)、35日龄(4组)断奶4个处理组,每个处理3个重复,每个重复1窝,定时定点制杀取十二指肠、空肠上段、空肠下段、回肠作组织切片,观察其绒毛高度、隐窝 深度、绒毛宽度、肠壁厚度。结果表明,仔猪十二指肠、空肠上段、空肠下段和回肠的绒毛高度、隐窝深度、绒毛宽度、肠壁厚度均有不同程度的变化;断奶日龄越早,仔猪肠粘膜变化越大,其中绒毛高度下降幅度越大,恢复时间越长,一般在断奶后8-12d降至最低,随后恢复较快。1组仔猪十二指肠、空肠上段、回肠隐窝深度显著下降;绒毛宽度各组各部位均呈先变窄后变宽趋势,肠壁厚度以1组变化明显。从小肠各部位比较,绒毛高度十二指肠最高,空肠上段次之;隐窝深度、绒毛宽度也以十二指肠最深或最宽;肠壁厚度以回肠最厚。仔猪在29日龄时各组各部位肠粘膜指标降至最低水平,之后,随着日龄的增加,各指标增加显著。  相似文献   

5.
应用H.E.染色对麋鹿十二指肠、空肠和回肠的组织结构进行了观察。结果显示,麋鹿小肠肠壁由内向外依次为黏膜层、黏膜下层、肌层和浆膜层,肌层由内层环形肌和外层纵形肌构成。黏膜层向肠腔内形成环形皱襞,黏膜厚度从十二指肠到回肠逐渐降低。黏膜层由内向外分为黏膜上皮、固有层和黏膜肌层,黏膜上皮和固有层向肠腔内突起形成肠绒毛,上皮下陷至固有层形成肠隐窝。十二指肠和空肠的肠绒毛高而密集,分别比回肠高46.26%(P0.05)和40.95%(P0.05),而回肠的隐窝深度比十二指肠低32.89%(P0.05),比空肠低19.17%(P0.05)。从十二指肠到回肠绒毛高度/隐窝深度的比值差异不显著(P0.05)。本试验为今后研究麋鹿消化道疾病的诊断、防治提供了科学依据。  相似文献   

6.
采用组织学和图像分析技术,研究了小鼠经腹腔注射交感神经损毁剂6-羟多巴胺后小鼠体重和小肠绒毛长度、V/C比值、绒毛宽度以及黏膜厚度的变化。结果表明,注射6-羟多巴胺组与对照组小鼠相比,体重下降(约3.4%);小肠绒毛变短(5.6%~19.4%),V/C比值减少(0.88%~24.1%),绒毛变窄(8.8%~24.7%)、黏膜厚度变薄(4.4%~16.9%),其中空肠的绒毛长度和黏膜厚度减少较明显,而十二指肠和回肠的V/C比值和绒毛宽度减少较明显。提示交感神经的活动可影响小肠的黏膜结构,从而影响肠道的消化吸收功能。  相似文献   

7.
采用组织学方法研究了1~8周龄马龙深沟鸡十二指肠形态学结构的发育过程。结果表明:1~8周龄马龙深沟鸡十二指肠绒毛高度和宽度随周龄增加而逐渐增长,而肠腺隐窝深度和肠腺密度呈下降趋势,绒毛高度与隐窝深度的比值不断增大;肠腺的宽度变化较小,4周龄肠绒毛基部开始出现淋巴小结;肌层发达,由内环和外纵2层平滑肌组成,环肌和纵肌层厚度的变化不同,环肌层较厚,1~8周龄厚度呈递减趋势,纵肌薄,厚度稳定在14~17μm。  相似文献   

8.
为研究日粮中添加天蚕素、合生素对AA肉鸡小肠黏膜形态及免疫细胞的影响,试验选用480只1日龄健康AA肉鸡,随机分为4组,每组4个重复,每个重复30只鸡。对照组(Ⅰ组)饲喂基础日粮,试验组(Ⅱ~Ⅳ组)分别在基础日粮中添加0.5%天蚕素、0.3%合生素、0.5%天蚕素+0.3%合生素。试验期42 d。结果显示,与Ⅰ组相比,Ⅳ组十二指肠、回肠绒毛高度极显著升高(P<0.01);Ⅱ组空肠和回肠、Ⅲ组小肠各段隐窝深度显著或极显著降低(P<0.05;P<0.01);Ⅱ、Ⅲ组小肠各段和Ⅳ组十二指肠、回肠绒毛高度/隐窝深度显著或极显著升高(P<0.05;P<0.01);Ⅱ组空肠绒毛宽度显著降低(P<0.05),Ⅲ组十二指肠和空肠、Ⅳ组空肠和回肠绒毛宽度均极显著降低(P<0.01);Ⅱ、Ⅲ组空肠黏膜厚度显著升高(P<0.05),Ⅳ组小肠各段黏膜厚度均极显著升高(P<0.01)。Ⅱ组回肠上皮内淋巴细胞数量显著升高(P<0.05),Ⅲ组十二指肠、Ⅳ组小肠各段上皮内淋巴细胞数量极显著升高(P<0.01);Ⅲ组十二指肠、Ⅳ组十二指肠和回肠杯状细胞数量极显著升高(P<0.01);Ⅳ组空肠肥大细胞数量显著升高(P<0.05)。综上所述,天蚕素、合生素单独或联合添加均能改善AA肉鸡小肠黏膜结构,促进小肠黏膜免疫细胞增殖,联合添加效果最佳。  相似文献   

9.
7日龄断奶长&#215;大仔公猪,以代乳粉配制代乳液饲养。12头仔猪9日龄按体重随机均分为对照组(基础日粮)和甘草次酸(GA)组(代乳粉添加200 mg/kg的GA),每组6头。连续饲喂12 d。仔猪21日龄称重后处死,测定内脏指数、小肠黏膜上皮细胞蛋白质含量、空肠和回肠黏膜绒毛高度、隐窝深度和固有层厚度以及空肠上皮细胞鸟氨酸脱羧酶活性。结果表明,与对照组相比,GA组肝脏指数、小肠质量/长度比、空肠和回肠绒毛高度及回肠隐窝深度显著增加(P〈0.05),空肠上皮细胞鸟氨酸脱羧酶活性显著增强(P〈0.01)。提示GA促进了仔猪小肠和肝脏的发育,改善黏膜结构;提高上皮细胞鸟氨酸脱羧酶活性可能是GA影响小肠发育的分子机制之一。  相似文献   

10.
以藏鸡和来航鸡为研究对象,探究高原和平原环境中小肠黏膜结构的形态特征差异。采用计量组织学和图像分析法,对3只藏鸡和3只来航鸡不同肠段黏膜结构的形态特征进行比较。结果表明,来航鸡小肠绒毛的高度都高于藏鸡,空肠和回肠间差异极显著(P<0.01),十二指肠间差异不明显;来航鸡小肠绒毛的宽度都极显著大于藏鸡(P<0.01);来航鸡十二指肠的肠绒毛隐窝深度极显著大于藏鸡(P<0.01),空肠和回肠间的差异不明显;来航鸡小肠绒毛肌层厚度都极显著大于藏鸡(P<0.01);来航鸡的消化吸收功能强于藏鸡。  相似文献   

11.
Forty eight 21 days old piglets were used to compare the effect of prebiotic or probiotic supplementation and ileo rectal anastomosis on the morphology of the small intestine. Half of the piglets were maintained intact and the other half was subjected to an ileo rectal anastomosis (IRA). Each group of piglets received one of the following diets: 1) basal diet (C), 2) basal diet supplemented with a Xylo-oligosaccharide (XOS), 3) basal diet supplemented with a Saccharomyces cerevisiae (SC) and 4) basal diet supplemented with XOS and SC. Villus height was greatest with XOS and with XOS + SC, only in the ileum, as compared to controls. In the duodenum, crypt width was highest in the control group, but no significant differences were found in the jejunum and ileum. The IRA piglets had longer villi in the jejunum and shorter villi in the ileum. The crypt depth was greater in the duodenum and in the ileum of IRA piglets. Villus height/crypt depth was lower in the duodenum and in the ileum, in the IRA piglets. In conclusion, the XOS, but not the SC, moderately modified the intestinal morphology. The IRA modified the intestinal villus and crypt architecture but its consequence on the absorption of nutrients needs to be investigated.  相似文献   

12.
This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa) on intestinal absorption and barrier function, intestinal morphology and antioxidant capacity of 7 days old piglets. Twenty-four 7 days old piglets were allotted to four treatments:control group (artificial milk), STa group (artificial milk +2×109 CFU E.coli LMG194-pBAD-STa), LMG194 group (artificial milk +2×109 CFU E.coli LMG194), and K88 group (artificial milk +2×109 CFU E.coli K88).The pigs were treated with E.coli on the 5th day and slaughtered on the 7th day. The results showed that, compared with the control group, villus height in jejunum, ileum and duodenum, crypt depth and villus height/crypt depth in duodenum, and small intestine villi surface area were significantly decreased in STa group (P<0.05). The activities of catalase (CAT) in ileum and colon, total nitric oxide synthase (TNOS) in serum, ileum, jejunum and colon, inducible nitric oxide synthase (iNOS) in colon were significantly decreased in STa group (P<0.05). STa group also had a higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in serum (P<0.05). These results suggested that recombinant E.coli expressing STa could lead to intestinal injury and oxidative stress of 7 days old piglets.  相似文献   

13.
吕阳  张林  李雪妮  赵迪  吴涛 《中国畜牧兽医》2017,44(9):2816-2821
试验旨在研究表达耐热肠毒素(STa)的重组大肠杆菌对7日龄仔猪肠道形态结构及抗氧化功能的影响。选取24头7日龄仔猪,随机分在4个日粮处理组,分别为对照组(人工乳),STa组(人工乳+2×109 CFU重组菌LMG194-pBAD-STa),LMG194组(人工乳+2×109 CFU大肠杆菌LMG194),K88组(人工乳+2×109 CFU大肠杆菌K88)。试验第5天进行攻毒,第7天屠宰取样,测量小肠黏膜的绒毛高度和隐窝深度,检测空肠、回肠、结肠及血清中的过氧化氢酶(CAT)、总一氧化氮合成酶(TNOS)与诱导型一氧化氮合酶(iNOS)的活力及血清中丙二醛(MDA)与过氧化氢(H2O2)的含量。试验结果表明,与对照组相比,STa组仔猪各肠段绒毛高度均显著降低(P<0.05),十二指肠隐窝深度及绒毛高度与隐窝深度的比值显著降低(P<0.05),十二指肠、空肠、回肠绒毛表面积显著降低(P<0.05);同时,STa组回肠、结肠CAT活力显著降低,血清、空肠、回肠和结肠中的TNOS活力显著降低(P<0.05),STa组结肠iNOS活力显著降低(P<0.05),STa组血清中的MDA与H2O2含量显著提高(P<0.05)。结果显示,表达STa的重组大肠杆菌可导致7日龄仔猪肠道结构损伤和抗氧化能力下降。  相似文献   

14.
本试验旨在研究丁酸钠对断奶羔羊胃肠道发育的影响。选取24只42日龄的断奶羔羊,随机分成4组,每组6个重复。空白对照组饲喂基础饲粮,抗生素组饲喂在基础饲粮中添加250 mg/kg黄霉素的饲粮,丁酸钠A组和丁酸钠B组分别饲喂在基础饲粮中添加2和3 g/kg包膜丁酸钠的饲粮。各组分别在试验第14天、第28天选取3只羔羊屠宰取样。结果表明:试验第14天时,各组断奶羔羊的瘤胃相对质量和相对容积以及各胃肠道形态指标均无显著差异(P>0.05);试验第28天时,与空白对照组相比,丁酸钠B组瘤胃背囊的肌层厚度,瘤胃腹囊的肌层厚度、乳头高度,十二指肠、空肠和回肠绒毛高度,空肠绒毛表面积以及空肠和回肠绒毛高度/隐窝深度显著增加(P<0.05)。由此可见,在饲粮中添加3 g/kg包膜丁酸钠并持续饲喂28 d可以促进断奶羔羊瘤胃乳头和肌层以及肠道绒毛的生长,增加肠道绒毛高度与隐窝深度的比值,有利于胃肠道的发育。  相似文献   

15.
为研制安全、高效环保型保育猪中药添加剂,将72头体重相近的28日龄杜长大断奶仔猪随机分为4组,每组3个重复,每个重复6头猪,分别饲喂基础饲粮、基础饲粮+抗生素、基础饲粮+1%中草药组方Ⅰ、基础饲粮+1%中草药组方Ⅱ,进行为期28 d的饲养试验,统计腹泻频率及腹泻指数、测定肠黏膜免疫细胞。结果显示,中药Ⅱ组效果优于中药Ⅰ组和抗生素组。中药Ⅱ组腹泻持续时间最短、腹泻频率及腹泻指数最低;与对照组相比,对回肠段杯状细胞数量影响最大的是抗生素组,提高14.29%(P〉0.05)。对其它肠黏膜免疫细胞数量影响最大的是中药Ⅱ组,提高十二指肠、空肠及回肠段上皮内淋巴细胞数量分别达23.37%(P〈0.01)、16.33%(P〈0.01)、14.52%(P〈0.01);提高十二指肠及空肠段杯状细胞数量分别达31.82%(P〈0.01)、26.92%(P〈0.01);提高十二指肠、空肠及回肠段肥大细胞数量分别达17.99%(P〈0.01)、23.87%(P〈0.01)、18.71%(P〈0.01)。  相似文献   

16.
In order to investigate the effects of cecropin and synbiotics on the intestinal mucosal morphology and intestinal mucosal immune cells of AA broilers. 480 of 1-day-old healthy AA broilers were chosen and randomly divided into 4 groups with 4 replicates per group and 30 broilers per replicate. The broilers in group Ⅰ(control group) were fed basal diet,while that in groups Ⅱ to Ⅳ were fed the basal diet supplemented with 0.5% cecropin,0.3% synbiotics,0.5% cecropin+0.3% synbiotics,respectively. The trial lasted for 42 days. The result showed that compared with group Ⅰ,the villus height of duodenum and ileum in group Ⅳ were extremely significantly increased (P<0.01).The crypt depth of jejunum and ileum in group Ⅱ,and that of small intestine in group Ⅲ were significantly or extremely significantly decreased (P<0.05;P<0.01).The villus height/crypt depth of small intestine in groups Ⅱ and Ⅲ,and that of duodenum and ileum in group Ⅳ were significantly or extremely significantly increased (P<0.05;P<0.01).The villus width of jejunum in group Ⅱ was significantly decreased (P<0.05),while the villus width of duodenum and jejunum in group Ⅲ,that of jejunum and ileum in group Ⅳ were extremely significantly decreased (P<0.01).The mucous thickness of jejunum in groups Ⅱ and Ⅲ were significantly increased (P<0.05),while that of small intestine in group Ⅳ was extremely significantly increased (P<0.01).The number of the intraepithelial lymphocyte of ileum in group Ⅱ was significantly increased (P<0.05),and that of duodenum in group Ⅲ and small intestine in group Ⅳ were extremely significantly increased (P<0.01).The number of the goblet cell of duodenum in group Ⅲ,duodenum and ileum in group Ⅳ were extremely significantly increased (P<0.01).The mast cell of jejunum in group Ⅳ was significantly increased (P<0.05).In conclusion,both the cecropin and synbiotics could improve the small intestine mucosal structure and promote the small intestine mucosal immunocompetent cells proliferation in AA broiler, and the effect of combined utilization would be better.  相似文献   

17.
本试验旨在研究开食料中性洗涤纤维(NDF)水平对羔羊消化道组织形态和消化酶活性的影响。选择健康的初生湖羊公羔[(初生重(3.01±0.12)kg]18只,随机分为3组,每组6只羊。对照组随母羊哺乳,试验组随母羊哺乳同时于7日龄开始补饲NDF水平分别为15.13%(高NDF水平组)和10.00%(低NDF水平组)的开食料。35日龄时屠宰。结果表明:1)高NDF水平组的瘤胃乳头长度、肌层厚度,网胃乳头长度、乳头宽度、肌层厚度,瓣胃瓣叶宽度、肌层厚度以及皱胃肌层厚度均极显著高于对照组(P<0.01),瓣胃瓣叶长度、皱胃黏膜层厚度均显著高于对照组(P<0.05)。高NDF水平组的瘤胃乳头长度、网胃肌层厚度均极显著高于低NDF水平组(P<0.01),瓣胃瓣叶宽度、肌层厚度以及皱胃黏膜层厚度均显著高于低NDF水平组(P<0.05)。2)高、低NDF水平组的十二指肠隐窝深度极显著低于对照组(P<0.01),高、低NDF水平组的十二指肠肌层厚度极显著高于对照组(P<0.01)。高NDF水平组的空肠绒毛长度极显著高于对照组和低NDF水平组(P<0.01),高、低NDF水平组的空肠隐窝深极显著低于对照组(P<0.01),高NDF水平组的空肠肌层厚度显著高于对照组(P<0.05)。高、低NDF水平组的回肠绒毛长度极显著高于对照组(P<0.01),高、低NDF水平组的回肠肌层厚度极显著高于对照组(P<0.01)。3)对照组的瘤胃蛋白酶活性极显著高于高、低NDF水平组(P<0.01),高NDF水平组极显著高于低NDF水平组(P<0.01);低NDF水平组的瘤胃α-淀粉酶活性显著高于对照组(P<0.05)。低NDF水平组的空肠α-淀粉酶活性显著高于对照组和高NDF水平组(P<0.05),低NDF水平组的十二指肠、空肠和回肠胰蛋白酶活性显著高于对照组(P<0.05)。由此可见,15.13%NDF水平的开食料有利于断奶前羔羊消化道的发育。  相似文献   

18.
The intestinal morphology of 7-week-old pigs was investigated by light (LM) and scanning electron microscopy (SEM). The piglets were fed either a semisynthetic or a cereal-based diet. The shapes of the intestinal villi and crypts of the duodenum, jejunum and ileum were examined. The villi were predominantly tongue-shaped. In the duodenum they were also ridged, branched and folded, and in the jejunum they were also leaf-like and ridged. At places with lymph follicles, the surface of the ileum was rugged with meandering fold-like villi. The crypts of the three segments of the small intestine were mainly coiled and sometimes branched. A novel morphometric evaluation method was introduced using the enlargement factors of each villus and crypt surface. The enlargement factor for the villus surface of the duodenum, jejunum and ileum was 3.13, 3.72 and 2.71, respectively. The factor for the crypt surface of the duodenum, jejunum and ileum was 9.07, 8.94 and 6.53, respectively. Furthermore, the relative proliferation rate and the epithelial renewal index were calculated for the first time. The relative proliferation rate of the duodenum, jejunum and ileum was 32.88, 34.78 and 50.77 proliferations per mm crypt perimeter, respectively. The diets consumed had an influence on the epithelial renewal index being higher for piglets fed the cereal-based diets.  相似文献   

19.
本试验旨在研究饲粮中添加2种植物提取物对断奶仔猪生长性能、肠道免疫功能、肠道形态结构和胰岛素样生长因子-Ⅰ(IGF?Ⅰ)含量的影响.选取胎次相近(3~6胎次)、25日龄断奶的"大白×长白"二元仔猪120头,随机分为4组,每组3个重复,每个重复10头.对照组饲喂基础饲粮,抗生素组在基础饲粮中添加100 mg/kg杆菌肽锌...  相似文献   

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