首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 62 毫秒
1.
提高萨福克羊超数排卵的效果研究   总被引：3，自引：0，他引：3  
对 5 4只 (次 )萨福克羊采用CIDR +FSH +PG法进行超数排卵 (以下简称超排 ) ,发情配种后第 6天从子宫角采胚 ,只均获胚数和只均获可用胚数分别为 (6 89± 2 6 2 )枚 (372 / 5 4 )和 (6 0 7± 2 2 8)枚 (32 8/ 5 4 )。并对不同国家产FSH ,注射LRH -A3 +P4,重复超排 ,左、右侧卵巢等影响萨福克羊超排效果的因素进行研究 ,结果表明 ,以加拿大和日本FSH超排效果最好 ,只均获可用胚分别为 (9 2 0± 1 6 4 )枚 (n =10 )和 (7 75± 1 6 7)枚 (n =16 ) ;注射LRH -A3 +P4组只均获可用胚(7 2 5± 1 18)枚 (n =16 ) ,极显著高于对照组 (4 11± 1 38)枚 (n =9) ;重复超排 (间隔 12月 )第 2次超排只均获可用胚(9 19± 3 39)枚 (n =16 ) ,明显高于第一次只均获可用胚 (6 0 0± 3 2 7)枚 (n =16 ) ;左右侧卵巢对萨福克羊超排效果无显著差异。  相似文献   

2.
青年布尔山羊超数排卵效果初探   总被引：4，自引：1，他引：3  
在非繁殖季节的七月,采用阴道栓+340 mg,FSH超数排卵,处理青年母羊7只,反应采胚7只,黄体平均数15.71±7.45个,卵泡平均数3.57±3.78个,平均采胚10.71±4.89枚,平均采可用胚8.86±6.36枚,可用胚率82.7%(62/75),胚胎回收率68.2%(75/110);在繁殖季节的八月下旬、九月、十月采用三种不同FSH剂量分别超排处理青年母羊57只、6只、5只分别取得了黄体均在30枚以上,采胚均数依次为18.07±8.33枚(n=56);26±8.10枚(n=6);22.6±12.80枚(n=5);平均采有效胚依次为18.04±8.32枚(n=56),25±6.36枚(n=6),18.6±12.22枚(n=5).可用胚率低次为99.8%(1010/1012)、96.2%(150/156)、82.3%(93/1 13),小剂量的FSH超排(200mg、320mg)与大剂量的FSH超排440mg)可用胚率差异高度显著(P＜0.01).  相似文献   

3.
波尔山羊胚胎移植试验   总被引：3，自引：1，他引：2  
应用FSH对8只供体波尔山羊进行了超排处理。在放栓的第9～12天,连续4d采用递减量肌肉注射FSH。结果有6只发情,同期发情率为75%。这6只羊配种后均采胚成功,共采胚83枚,只均13.8±7.36枚,其中可用胚75枚,只均12.5±5.99枚,可用胚胎率为90.36%(75/83)。将36枚可用胚移植受体奶山羊32只,妊娠18只,妊娠率56.25%(18/32)。  相似文献   

4.
22只波尔山羊供体用CIDR FSH PG 进行超排处理, 用手术法采胚,共获138枚可用胚, 头均获可用胚为6.27 ( 138/22) 枚,其中超排育成羊6只,只均回收可用胚5.50枚,经产母羊16只,只均回收可用胚6.57枚,经产羊平均回收胚胎总数高于育成羊;手术法移植78只受体羊,妊娠53只,妊娠率68.00%,产羔71只,其中产双胎的有18只受体,占妊娠总数的34.00%.  相似文献   

5.
 为探索适合草原放牧条件下西门塔尔牛的超数排卵技术,分别采用国产及进口促卵泡素(FSH)和三种方案（A：FSH+PG;B：FSH+PG+GnRH;C：FSH+PG+CIDR +GnRH）对放牧条件下西门塔尔牛进行超数排卵试验。结果表明,采用国产FSH超排时,方案A、方案B和方案C所获头均可用胚数分别为（3.43±2.41）枚、（5.93±3.54）枚和（7.21±3.19）枚,采用进口FSH超排时,三种超排方案所获头均可用胚数分别为（4.34±2.71）枚、（6.76±3.79）枚和（7.79±4.23）枚,采用两种FSH超排,方案B、方案C所获头均可用胚数明显高于方案A（P<0.05）;3种方案中,国产与进口FSH所获头均可用胚数差异均不显著（P>0.05）。试验表明,在放牧条件下,采用三种超排处理方案,以FSH+PG+CIDR+GnRH法最好,FSH+PG+GnRH法次之,FSH+PG法效果最差;国产FSH与进口FSH超排效果差异不显著。  相似文献   

6.
奶牛胚胎移植黄牛应用技术研究   总被引：3，自引：0，他引：3  
超排处理黑白花奶牛58头,有效47头,头均获卵7.2±5.2枚(340/47),可移胚4.7±4.3枚(220/47)。两种超排方法比较试验,低剂量预处理超排组头均获卵8.2±5.3枚(164/20),可移胚5.2±4.3枚(103/20),高于常规超排组头均获卵数6.5±5.1枚(176/27),可移胚数4.3±4.3枚(117/27)。鲜胚移植农户饲养的黄牛受体,其受胎率为44.6％(41/92)。  相似文献   

7.
对21只波尔山羊采用CIDR FSH PG方法进行超数排卵处理,发情配种后第6～7天采用常规外科手术法从子宫角采胚.结果平均获胚18.05枚/只,其中获可用胚15.76枚/只.另外,对不同季节、供体不同体况和卵巢不同发育情况等的超排效果进行比较,结果显示,秋季和春季超排平均获可用胚分别为1 6.70枚/只和13.50枚/只,秋季超排效果好于春季,但差异不显著(P＞0.05);体况好和体况差的供体平均获可用胚分别为17.67枚/只和12.20枚/只,差异显著(P＜0.05);卵巢发育情况良好和发育情况差的供体平均获可用胚分别为19.25枚/只和7.33枚/只,差异极显著(P＜0.01).  相似文献   

8.
用不同批号的FSH、PGF_(2a)共超排奶牛109头,超排有效率87.16%,采卵成功率90.35%,平均获卵数为7.55±6.62枚,平均获可用胚胎数5.67±5.22枚,不同批号间的平均获卵数,平均可用胚胎数差异均不显著(P>0.05)。供体超排后发情时间为36.30±9.92小时,受体同期发情处理后发情时间为63.24±16.09小时。奶牛移植受胎率为66.66%(6/9),黄牛移植受胎率为42.59%(23/54)。  相似文献   

9.
波尔山羊胚胎移植技术研究   总被引：5，自引：0，他引：5  
FSH－PG法超排供体波尔山羊14只，于发情第7日手术法子宫采卵。有超排反应14只，超排反应率100％，采卵成功13只，采卵成功率98.86%。获卵总数121枚，只均获卵9.31枚。可用胚119，胚胎可用率98.35%，只均可用胚9.15枚。以氯前列烯醇处理受体白山羊210只，72h内同期发性率97.11%。手术法子宫内鲜胚移植受体母羊116只，两情期移植成功率54.31％。产羔61只，产羔率96.82%。羔羊出生重平均4.26kg.  相似文献   

10.
研究主要针对CIDR放置时间长短对绵山羊超排效果的影响进行研究,以期为羊胚胎工程技术产业化提供坚实的基础.研究结果表明绵羊第10天起针的供体,平均回收数8.94枚±3.78枚,平均可用胚7.67枚±3.76枚,胚胎可用率90.34%.第13天起针超排的供体绵羊,平均回收8.38枚±3.79枚,平均可用胚6.37枚±3.03枚,胚胎可用率为76.03 %;第10天起针平均可用胚高于第13天起针的超排效果,两者差异显著(P＜0.05),两者之间平均回收胚基本一样,无显著差异(P＞0.05),但第10天起针的胚胎利用率高于第13天起针的胚胎可用率,差异极显著(P＜0.01).CIDR放置9天起针的供体山羊,平均可用胚为17.59枚±3.22枚,胚胎可用率87.40 %;CIDR放置15天起针的供体,平均可用胚数为12.73枚±4.01枚,胚胎可用率为67.95 %.CIDR放置9天起针平均可用胚高于CIDR放置15天起针的超排效果,差异极显著(P＜0.01),CIDR放置9天起针的胚胎可用率高于放置15天的胚胎可用率,差异极显著(P＜0.01).波尔山羊在11月、12月这种结果更明显;4月份虽然9天比15天高,但平均可用胚数和胚胎可用率之间没有差异(P＞0.05),而3月份平均可用胚差异显著(P＜0.05),胚胎可用率差异显著(P＜0.05).  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

14.
Tropical Animal Health and Production -  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

18.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号