Evaluation of a high-throughput nucleic acid extraction method for the detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples by PCR     

Nagaraja R. Thirumalapura  Willard Feria  Eric Hue  Corey Zellers  Deepanker Tewari 《Journal of veterinary diagnostic investigation》2021,33(2):375

Johne’s disease (paratuberculosis) is an economically important disease of cattle worldwide. The disease is caused by Mycobacterium avium subsp. paratuberculosis (MAP), a fastidious gram-positive bacterium. PCR is increasingly used in diagnostic laboratories for the detection of MAP in fecal samples given the rapid test turnaround time and sensitivity and specificity comparable to fecal culture. However, efficient extraction of DNA for sensitive detection of MAP by PCR is affected by the complex lipid-rich cell wall of MAP and the presence of PCR inhibitors in feces. We evaluated a high-throughput nucleic acid extraction method (MagMAX core nucleic acid purification kit with mechanical lysis module) in conjunction with an hspX gene PCR for the detection of MAP from bovine fecal samples, which resulted in correct identification of all negative (13 of 13) and positive (35 of 35) proficiency test samples obtained from the National Veterinary Services Laboratories. In addition, all 6 negative and 50 of 51 positive diagnostic specimens tested were categorized correctly.  相似文献   

Correlating Clinical Activity and Histopathologic Assessment of Gastrointestinal Lesion Severity: Current Challenges     

Michael Willard  Joanne Mansell 《Veterinary Clinics of North America: Small Animal Practice》2011,41(2):457-463

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Hepatic myelolipoma incarcerated in a peritoneopericardial diaphragmatic hernia in a cat     

RM Wouda  MD Chalkley  AR Fraser  PAE Moses 《Australian veterinary journal》2010,88(6):231-235

Hepatic myelolipoma incarcerated in a peritoneopericardial diaphragmatic hernia was diagnosed in an 11-year-old, desexed female Persian cat. The cat was initially referred for investigation of tachypnoea and dyspnoea. Peritoneopericardial diaphragmatic hernia is a common incidental finding in cats and is usually asymptomatic. Myelolipoma is an extremely rare benign tumour, composed of extramedullary haematopoietic cells and adipose tissue. Myelolipomas are hypothesised to result from metaplastic alteration, rather than a neoplastic process, although this theory cannot be substantiated. The present case is only the fourth report of such an unusual occurrence in cats and displays significant differences to previous reports. Hepatic entrapment and burgeoning of the mass within the pericardial sac resulted in cardiac tamponade and overt signs of right-sided cardiac failure. Surgical intervention was successful and despite concerns regarding the cat's clinical presentation and the gross appearance of the lesion(s), a good long-term outcome is anticipated.  相似文献   

Opportunistic Invasive Cutaneous Fungal Infections Associated with Administration of Cyclosporine to Dogs with Immune‐mediated Disease          下载免费PDF全文

B.B. McAtee  K.J. Cummings  A.K. Cook  J.A. Lidbury  J.C. Heseltine  M.D. Willard 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(6):1724-1729

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Letter to the Editor          下载免费PDF全文

P.R. Fox DVM  DACVIM  M.D. Kittleson DVM  PhD  DACVIM  C. Basso MD  PhD  FESC  G. Thiene MD  FCRP  FESC 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(4):969-969

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Efficacy of Omeprazole versus High‐Dose Famotidine for Prevention of Exercise‐Induced Gastritis in Racing Alaskan Sled Dogs     

K.K. Williamson  M.D. Willard  M.E. Payton  M.S. Davis 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2010,24(2):285-288

Background: Omeprazole and famotidine both reduce severity of exercise‐induced gastritis, but administering famotidine is easier than administering omeprazole during racing competition. Hypothesis: Famotidine is more efficacious than no treatment in reducing severity of exercise‐induced gastritis; and high‐dose famotidine is more efficacious than omeprazole in reducing severity of exercise‐induced gastritis. Animals: Experiment 1: Randomized placebo‐controlled study, 36 sled dogs (3–8 years); Experiment 2: Randomized positive‐control study, 52 sled dogs (2–8 years). Methods: Experiment 1: Equal numbers of dogs randomly assigned to famotidine (20 mg q24h) or no treatment groups. Gastroscopy was performed 24 hours after the dogs ran 330 miles. Mucosal appearance was blindly scored by previously described scoring system. Experiment 2: Equal numbers of dogs randomly assigned to omeprazole (20 mg q24h) or high‐dose famotidine (40 mg q12h) groups. Gastroscopy was performed 48 hours before and 24 hours after the dogs ran 300 miles. Mucosal appearance was blindly scored by previously described scoring system. Results: Famotidine reduced the prevalence of clinically relevant, exercise‐induced gastric lesions compared with no treatment (7/16 versus 11/16, P= .031). Compared with high‐dose famotidine, omeprazole significantly decreased the severity (0.4 versus 1.2, P= .0002) and prevalence (2/23 versus 7/21, P= .049) of gastric lesions. Conclusions and Clinical Relevance: Although famotidine provides some benefit in the prevention of exercise‐induced gastric lesions, omeprazole is superior to famotidine in preventing gastritis in dogs running 300 miles. Routine administration of omeprazole is recommended to prevent stress‐associated gastric disease in exercising and racing Alaskan sled dogs.  相似文献   

FIELD CONFERENCE OF PENNSYLVANIA GEOLOGISTS     

Willard B 《Science (New York, N.Y.)》1933,77(2009):629-630

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PIT标记技术在幼鲵标记中的应用研究   总被引：1，自引：0，他引：1  

王启军  赵虎  ＡｎｄｙＫｏｕｂａ  ＳｃｏｔｔＴ．Ｗｉｌｌａｒｄ  张红星  陈玖安 《水生态学杂志》2011,32(2):146-148

对347尾预放流幼鲵的尾部皮下层和肌肉层分别进行横向和纵向PIT标记,通过对标记幼鲵6个月的跟踪观察,共收集到脱落芯片94枚,总脱落率高达27.1%,严重影响标记目的。对植入芯片时的记录进行对比分析,得出尾部皮下层纵向植入法对幼鲵肌体伤害最小,脱落率最低,为3.0%,笔者认为该方法是PIT标记幼鲵的最佳方法。  相似文献   

Treatment of Trochanteric Ulcers with Cranial Sartorius and Rectus Femoris Muscle Flaps     

JONATHAN N. CHAMBERS DVM  Dipiomate ACVS    PAULT PURINTON DVM  PhD    JAMES L. MOORE MD  SHEILA W. ALLEN DVM  MS  Dipiomate ACVS 《Veterinary surgery : VS》1990,19(6):424-428

Five dogs with pressure ulcers over the greater trochanter were treated by debridement and transposition of the cranial part of the sartorius or the rectus femoris muscle. Both muscles had vascular anatomy that allowed transposition based on a major vascular pedicle. All wounds healed promptly by primary intention and no ulcer recurred. The cranial sartorius flap technique was easier to perform than the rectus femoris technique.  相似文献   

Implantation of the Glasgow bioprosthetic heart valve in sheep     

J. Reid Ph.D.  MRCVS.    D.J. Wheatley MD  FRCS.  T.J. Spyt FRCS. 《Veterinary anaesthesia and analgesia》1986,14(1):188-196

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