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1.
基于CuFe2O4纳米粒子能显著增强Luminol-EDTA体系的发光,首次建立了Luminol-EDTA-CuFe2O4 NPs化学发光新体系。紫外吸收光谱和化学发光光谱表明纳米CuFe2O4注入Luminol-EDTA体系后,未生成新发光物质,结合纳米CuFe2O4的特性,提出了CuFe2O4 NPs参与Luminol-EDTA体系可能的发光机理。研究发现芦丁能抑制Luminol-EDTA-CuFe2O4 NPs体系的化学发光,结合流动注射技术,将此化学发光体系应用于芦丁片中芦丁含量的测定。在优化实验条件下,芦丁浓度在2×10-8~2×10-5 mol/L范围内芦丁浓度的对数和相对化学发光值呈线性,芦丁浓度检出限（LOD）为1.21×10-9 mol/L。将本方法应用于芦丁片中的芦丁含量测定,回收率为97%~102%,RSD为2.54%（c=1×10-7mol/L,n=11）。 相似文献
2.
大豆苗期耐盐性的简便鉴定方法 总被引：1，自引：0，他引：1
盐胁迫是影响大豆产量的重要非生物胁迫因素,筛选耐盐种质资源对培育耐盐大豆品种具有重要的意义。本研究利用4个耐盐品种和4个盐敏感品种进行苗期耐盐性鉴定,以蛭石为培养基质,在大豆真叶展开时,分别用100、200和250 mmol L–1的NaCl溶液处理,每2 d浇一次盐溶液,每天测定大豆真叶SPAD值。盐处理8 d后,分别取各品种的根、茎、叶,用原子吸收光谱仪测定其Na+含量。结果表明,用200 mmol L–1 NaCl处理的耐盐品种和盐敏感品种表型差异明显,但叶片Na+含量差异不显著;用250 mmol L–1 NaCl处理后,表型差异明显且叶片Na+含量差异显著。200 mmol L–1和250 mmol L–1 NaCl处理下叶片失绿明显,盐敏感品种叶片积累的Na+含量与SPAD值极显著负相关。本研究建立了一种以蛭石为基质,用200 mmol L–1或250 mmol L–1 NaCl处理,8 d即可进行大豆苗期耐盐性鉴定的简便方法,为大豆种质资源苗期耐盐性的大规模鉴定及耐盐品种选择和耐盐机理的研究提供了方法。 相似文献
3.
采用一步溶剂热合成法制备了镍钴硫化物纳米微球,利用X射线衍射光谱（XRD）、扫描电子显微镜（SEM）和X光电子能谱（XPS）对制备的材料进行了表征。采用循环伏安和恒电流充放电等电化学方法考察了镍钴硫化物用作超级电容器的电极材料的性能。160℃制备的材料在2 mA/cm2的电流密度下,测得的质量比电容和面积比电容分别为1 298 F/g和1.13 F/cm2。在电流密度为10 mA/cm2下,经过10 000圈的循环稳定性测试后,比电容保持了原来的70.8%。此外,通过对反应温度的控制,研究了反应温度对其超级电容器性能的影响。结果表明,反应温度对生成物的形貌几乎没有什么影响,对其电化学性能有一定的影响。 相似文献
4.
以叶片衰老快慢不同的两个棉花品系L21和L22为材料,研究了NaCl胁迫对棉花叶片衰老的影响及其相应的生理学机制。温室内水培棉苗,待第5片真叶展开20 d后用含125 mmol·L-1 NaCl的营养液处理棉苗,以不含NaCl的营养液处理为对照。结果显示,NaCl胁迫下L21和L22叶片中叶绿素含量和光合作用速率下降,叶片和根中的Na+含量上升、K+含量降低;NaCl胁迫还增加了棉株体内脱落酸（ABA）含量、降低了玉米素核苷（ZR）含量。表明K+含量降低以及ABA含量升高、ZR含量下降是NaCl胁迫促进棉花叶片衰老的重要原因。 相似文献
5.
研究了Ca2+ 对NaCl胁迫下蚕豆气孔运动及质膜K+通道的影响。结果表明,100 mmol L-1 NaCl可明显诱导气孔开放,该现象可被10 mmol L-1 CaCl2 显著抑制。为探讨盐胁迫下Ca2+对K+和Na+跨膜运输的调控机制,我们利用膜片钳技术记录全细胞K+ 电流发现,在100 mmol L-1 NaCl胁迫下,加入10 mmol L-1 CaCl2胞外处理,显著抑制质膜K+内向及外向通道电流,这种抑制可被1 mmol L-1 La3+ (Ca2+通道抑制剂)缓解。非盐胁迫下,10 mmol L-1 CaCl2 胞外处理也能显著抑制质膜内向K+通道,但明显激活其外向通道,加入1 mmol L-1 La3+并不能被缓解。用H2O2专一的荧光探针二氯荧光素二乙酸酯(H2DCF-DA)单细胞分析保卫细胞内H2O2含量变化显示,在100 mmol L-1 NaCl盐胁迫下,10 mmol L-1 CaCl2 处理明显诱导H2O2在保卫细胞中积累;100 mmol L-1 NaCl和10 mmol L-1 CaCl2单独处理并不能诱导H2O2积累。推测Ca2+在盐胁迫下可能先诱导H2O2在胞内积累,进而激活质膜Ca2+通道,迅速提高胞内Ca2+浓度以抑制Na+通过质膜K+通道跨膜内流,同时调节Na+外流,两种效应共同作用促使气孔关闭,减少盐胁迫下水分的过度散失。上述结果将为Ca2+调控作物抗盐机制研究提供新的思路。 相似文献
6.
以掺天然砂砾改良的红粘土为研究对象,采用室内承载板法测定回弹模量,通过不同初始干密度下的回弹模量试验,深入研究了天然砂砾改良红粘土的回弹模量随初始干密度的变化规律,建立了不同初始干密度下回弹模量的预估模型。试验结果表明:在同一天然砂砾掺量下,随着初始干密度的提高,红粘土的回弹模量逐渐增大。当初始干密度由低向最大干密度增大时,回弹模量增长速度较快,当初始干密度超过最大干密度继续增大时,回弹模量增长速度变缓;在同一初始干密度下,当天然砂砾掺量从0增至30%时,回弹模量的增长幅度较小,当天然砂砾掺量超过30%而继续增长时,回弹模量增长幅度较大;初始干密度由1.6 g·cm-3增长至2.0 g·cm-3时,回弹模量增长值随天然砂砾掺量的增加而逐渐增大。分别建立了回弹模量随初始干密度及天然砂砾掺量变化的预估模型,通过补充试验,验证了预估模型的准确性。 相似文献
7.
南疆棉区杂交棉高产栽培优化配方施肥技术研究 总被引：4，自引：1，他引：3
采用“3414”不完全设计方法,研究了南疆杂交棉高产栽培水平下氮磷钾肥料的优化配比效果。结果表明：氮磷钾肥料的主效应对杂交棉皮棉产量的影响顺序为钾肥＞磷肥＞氮肥;氮磷钾肥料两因素间的交互作用,对杂交棉皮棉产量影响的大小顺序是N×K＞P×K＞N×P;根据氮磷钾肥料组合寻优结果和杂交棉高产棉田的实践,在南疆种植杂交棉要获得2836～3050 kg·hm-2的皮棉产量,需施纯N 262.3～748.5 kg·hm-2,P2O5 14.1～277.0 kg·hm-2,K2O 43.6～199.8 kg·hm-2;最佳经济施肥量分别为：纯N 433.9 kg·hm-2,P2O5 116.5 kg·hm-2,K2O 103.0 kg·hm-2,N∶P2O5∶K2O=1∶0.27∶0.24。 相似文献
8.
河南植棉区施氮量对麦棉两熟产量及氮肥利用率的影 响简 总被引：3，自引：2，他引：1
研究了河南植棉区麦棉两熟施氮量对两季产量和氮肥利用率的影响。结果表明,施氮能显著提高小麦产量、总有效穗数和穗粒数,籽粒千粒重随施氮量增加而降低;商丘和内黄试验点小麦最高产量所需的施氮量分别为201.4 kg·hm-2和187.2 kg·hm-2,经济最佳施氮量分别为163.0 kg·hm-2和134.9 kg·hm-2。施氮也能显著提高棉花产量和单株成铃数;适量施氮可提高铃重;商丘和内黄试验点棉花最高产量所需的施氮量分别为244.4 kg·hm-2和224.2 kg·hm-2,经济最佳施氮量分别为225.9 kg·hm-2和207.0 kg·hm-2。小麦氮肥利用率以施氮量180 kg·hm-2最高。麦棉两季氮肥利用率,商丘试验点随施氮量增加而降低;内黄试验点以施氮量390 kg·hm-2最高。小麦、棉花氮肥偏生产力、农学利用率均随施氮量增加而降低。 相似文献
9.
2007—2008年对宁粳1号和宁粳3号的丰产示范方进行调查,2008年以宁粳3号为材料进行氮肥用量和前后比例试验,研究常规粳型超级稻超高产群体特征及对氮的响应。结果表明,常规粳型超级稻宁粳1号和宁粳3号抽穗后干物质积累占籽粒产量的70%~80%,茎叶等营养器官的表观转运率少,易高产稳产。足够的颖花量是高产稳产的保证,要达到11.0 t hm-2以上的产量,颖花数要 ≥ 42 000 m-2;要达到11.7 t hm-2以上的产量,颖花数要 ≥ 45 000 m-2,同时结实率 ≥ 90%,粒重 ≥ 26 mg。超高产群体抽穗期适宜叶面积指数(LAI)为7.0~7.5,叶色呈“黑黄”节奏变化,后期生长速率(CGR)高,收获指数(HI)≥ 0.5。氮肥的适量施用和适当后移,不仅可以保证宁粳1号和宁粳3号生育期“黑黄”节奏变化,建立抽穗期适宜LAI的群体,还可以保持超高产株型特征,提高抽穗后LAI、叶面积维持期、CGR和HI,最终实现超高产量。 相似文献
10.
11.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary
K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes. 相似文献
12.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans. 相似文献
13.
Hongyan WEI Jian ZHOU Jinguang LU Jingzheng SONG Qiongxi YU Zhihong JIANG 《Medicinal Plant》2019,(6):27-29
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol... 相似文献
14.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed. 相似文献
15.
D. A. Bond G. J. Jellis G. G. Rowland J. Le Guen L. D. Robertson S. A. Khalil L. Li-Juan 《Euphytica》1993,73(1-2):151-166
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses. 相似文献
16.
Chunli TANG Fengxian ZHAO Hongxia CHEN Jiabao MA Jiangcun WEI Meiyan QIU Zhen XIE 《Medicinal Plant》2019,(6):60-65
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination... 相似文献
17.
Mohammed Riyazaddin Polavarapu B. Kavi Kishor Are Ashok Kumar Belum V. S. Reddy Rajendra S. Munghate Hari C. Sharma 《Plant Breeding》2015,134(4):423-436
Sorghum shoot fly, Atherigona soccata, is one of the important pests of postrainy season sorghums. Of the 90 sorghum genotypes evaluated for resistance to this pest, RHRB 12, ICSV 713, 25026, 93046 and 25027, IS 33844‐5, Giddi Maldandi and RVRT 3 exhibited resistance in postrainy season, while ICSB 463, Phule Anuradha, RHRB 19, Parbhani Moti, ICSV 705, PS 35805, IS 5480, 5622, 17726, 18368 and 34722, RVRT 1, ICSR 93031 and Dagidi Solapur showed resistance in rainy season, suggesting season‐specific expression of resistance to A. soccata. ICSB 461, ICSB 463, Phule Yasodha, M 35‐1, ICSV 700, 711, 25010, 25019 and 93089, IS 18662, Phule Vasudha, IS 18551 and 33844‐5 and Barsizoot had fewer deadhearts than plants with eggs across seasons, suggesting antibiosis as one of the resistance mechanism. Five genotypes exhibited resistance with high grain yield across seasons. Correlation, path and stepwise regression analyses indicated that leaf glossiness, seedling vigour, trichome density, oviposition and leaf sheath pigmentation were associated with the expression of resistance/susceptibility to shoot fly, and these can be used as marker traits to select and develop shoot fly‐resistant sorghums. 相似文献
18.
E. Keep 《Euphytica》1986,35(3):843-855
Summary Cytoplasmic male sterility (cms) is described in the F1 hybrids Ribes × carrierei (R. glutinosum albidum × R. nigrum) and R. sanguineum × R. nigrum. In backcrosses to R. nigrum, progenies with R. glutinosum cytoplasm were either all male sterile, or segregated for full male fertility (F) and complete (S) and partial (I) male sterility. Ratios of F:I+S suggested that two linked genes controlled cms, F plants being dominant for one (Rf
1) and recessive for the other (Rf
2).Segregation for cms in relation to three linded genes, Ce (resistance to the gall mite, Cecidophyopsis ribes), Sph
3(resistance to American gooseberry mildew, Sphaerotheca mors-uvae) and Lf
1(one of two dominant additive genes controlling early season leafing out) indicated that Rf
1and Rf
2were in this linkage group. The gene order and approximate crossover values appeared to be: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXafv3ySLgzGmvETj2BSbqef0uAJj3BZ9Mz0bYu% H52CGmvzYLMzaerbd9wDYLwzYbItLDharqqr1ngBPrgifHhDYfgasa% acOqpw0xe9v8qqaqFD0xXdHaVhbbf9v8qqaqFr0xc9pk0xbba9q8Wq% Ffea0-yr0RYxir-Jbba9q8aq0-yq-He9q8qqQ8frFve9Fve9Ff0dme% aabaqaciGacaGaamqadaabaeaafaaakeaacaWGdbGaamyzamaamaaa% baGaaiiiaiaacccacaGGWaGaaiOlaiaacgdacaGG0aGaaiiiaiaacc% caaaGaaiiiaiaacccacaGGGaGaamOuaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaaccdacaGGUaGaaiOmaiaacs% dacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaaaacaWGsbGaamOzaSGa% aGOmaOWaaWaaaeaacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccaaaGaamitaiaadAgaliaaigdakmaamaaa% baGaaiiiaiaacccacaGGGaGaaiiiaiaacccacaGGGaGaaiiiaiaacc% cacaGGGaGaaiiiaiaacccacaGGGaaaaiaadofacaWGWbGaamiAaSGa% aG4maaaa!6E4D!\[Ce\underline { 0.14 } Rf1\underline { 0.24 } Rf2\underline { } Lf1\underline { } Sph3\]. Crossover values of 0.36 for Ce-Lf
1, and 0.15 for Lf
1-Sph
3were estimated from the relative mean differences in season of leafing out between seedlings dominant and recessive for Ce and Sph
3.It is suggested that competitive disadvantage of lf
1-carrying gametes and/or zygotes at low temperatures may be implicated in the almost invariable deficit of plants dominant for the closely linked mildew resistance allele Sph
3. Poor performance of lf
1- (and possibly lf
2-) carrying gametes and young zygotes during periods of low temperature at flowering might also account for the liability of some late season cultivars and selections to premature fruit drop (running off). 相似文献
19.
[Objectives] To determine the optimum extraction technology for total phenols of leaves in Acanthopanax giraldii Harms.[Methods]The single factor test and ortho... 相似文献
20.
Summary
Hordeum chilense is a wild barley extensively used in wide crosses in the Triticeae. It could be a valuable source of resistance to Fusarium culmorum and Septoria nodorum. Some H. chilense x Triticum spp. amphiploids, named tritordeums, were more resistant than the parental wheat line to these diseases, others were not. Average contents of ergosterol and deoxynivalenol (DON) suggested that resistance to colonization by Fusarium was the highest for Hordeum chilense, followed by tritordeum and wheat in decreasing order. In particular, the H. chilense genotypes H7 and H17 enhanced the wheat resistance to F. culmorum in its tritordeum offsprings. Resistance to S. nodorum in tritordeum was not associated with tall plant height. There is sufficient genetic variation for resistance to F. culmorum and S. nodorum among tritordeum to allow the breeding of lines combining short straw and resistance to both diseases. 相似文献