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《中国兽医学报》2014,(8):1314-1318
经PCR检测南京某养殖场送检的表现为多系统衰竭综合征的家养野猪病原为猪圆环病毒2型(PCV2)。采用常规石蜡切片法和免疫组织化学法对患病仔猪的病理组织学变化特点及PCV2抗原在淋巴结内的分布进行了研究。结果显示,剖检病死仔猪病变主要表现为全身淋巴结肿大和间质性肺炎。组织病理学变化主要表现为淋巴结、脾和扁桃体等免疫器官淋巴滤泡萎缩,淋巴细胞数量减少,有大量巨噬细胞浸润及多核巨细胞;非淋巴组织病变主要为肺脏呈典型的间质性肺炎,肝脏局灶性坏死及淋巴细胞浸润;肺脏和肝脏病灶内也可见少量多核巨细胞。PCV2主要分布于淋巴组织内单核巨噬细胞、支气管黏膜上皮、肺泡壁Ⅱ型上皮细胞内,肾小球内也可见少量分布。 相似文献
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为探究猪圆环病毒3型(PCV3)在感染猪组织器官中的分布规律及其对猪组织产生的病理损伤变化,对广西某地区PCV3感染猪进行临床症状和病理学观察,发现单独感染PCV3病猪呈现典型的断奶仔猪多系统衰弱综合征(PMWS)症状,镜检发现病例呈现坏死性心肌炎、间质性肺炎、轻度的淋巴细胞性肝炎及免疫器官淋巴细胞消耗型炎症。免疫组化法检测结果发现淋巴组织中信号最强,PCV3阳性信号主要位于单核和巨噬细胞胞质中;PCV3可单独引发仔猪断奶衰竭综合征且23个组织中均有病毒分布。 相似文献
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我国自1997年报道断奶后仔猪多系统衰竭综合症(PMWS)以来,近年来与猪圆环病毒Ⅱ型(PCV-2)相关的PMWS在各地不断被发现,给我国的养猪业造成了相当大的经济损失.潍坊是山东省养猪较集中的地区,近年猪病的发生多而复杂,为了搞清潍坊地区PMWS的发生情况,我们在1年左右的时间内收集了8个县区PMWS疑似病例,并对其进行了尸体剖检、组织病理学检查和病原学检测.结果证明,潍坊地区PMWS的主要病原是PCV-2与猪繁殖与呼吸综合征病毒(PRSSV),还继发有多杀性巴氏杆菌、胸膜炎放线杆菌、链球菌、大肠杆菌、支原体、双芽巴贝斯焦虫、猪附红细胞体等多种病原的感染.本文就本病的诊断情况报告如下. 相似文献
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本研究凭借临床症状、组织病理学观察和尸体剖检综合诊断了1例黄牛狂犬病.发病牛临床症状表现为流涎,头颈后仰,四肢僵直,顶撞其他牛.尸体剖检可见大网膜、心内外均散在或密布出血点,左右心室腔积有大量血凝块.脑充血和水肿,脑膜静脉扩张.组织病理学检查可见脑血管严重充血,脑神经细胞多数自溶,在小脑的浦肯野细胞浆、海马多形细胞浆和脑干多极运动神经细胞浆中可见大小不等的圆形或椭圆形嗜酸性狂犬病病毒包涵体(内基氏小体).出现非化脓性脑炎,在部分小血管周围有大量淋巴细胞和少量巨噬细胞浸润,形成淋巴细胞性"管套".其他组织器官无明显病变. 相似文献
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猪断奶后多系统衰竭综合征的流行及防制对策 总被引：6，自引：0，他引：6
自Tischer (1 974)分离出猪圆环病毒 (PorcineCircovirus,PCV)以来 ,现已知PCV有PCV 1和PCV 2 2个血清型。PCV 2是猪断奶后多系统衰竭综合征 (PostweaningMultisystemicWastingSyndrome,PMWS)的主要病因。PMWS主要表现为精神、食欲不振 ,进行性呼吸困难、消瘦 ,贫血 ,皮肤苍白 ,肌肉无力 ,黄疸 ,死亡 ,体表淋巴结肿大。病理学特征主要表现全身器官组织的炎症变化 ,出现肝炎 ,多灶性间质性肺炎、肾炎 ,多灶性心肌炎 ,胃溃疡等。病毒可感染多种组织细胞 ,并形成合胞体性多核巨细胞及细胞质内包涵体。本文对PCV 2及与其密切相关的PMWS近几年来的研究成果进行了综述 相似文献
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<正>1如何诊断猪群中发生PMWS目前对于该病的诊断有三个标准,一是临床症状,二是典型的淋巴结病变,三是病变组织中有PCV2存在。当然,这几条标准仍然具有争议性,但起码给生产一线的养猪业者提供了诊断的依据。也有组织提出了几条相对详细的确诊标准。标准1∶整个猪群内出现相关症状。PMWS发生的特征是断乳仔猪的死亡率和系统衰竭出现的概率跟以往相比大大增加。如果没有过去的数据可以用来比较,可以将死亡率是否高于地区或全国水平的50%作为诊断标准。标准2∶病例剖检诊断PMWS。每一猪群至少要剖检5头病死猪,且至少有一头具有PMWS的典型的病变。从以上两点可以得知,有学者正试图从统计学角度来作为诊断PMWS的标准。但从该病实际发病情况来看,以数据来建立判定PMWS的标准是有一定困 相似文献
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猪断奶后多系统衰竭综合征（PMWS）是一种由猪圆环病毒2型（porcine circovirus 2,PCV-2）引起的新病。猪圆环病毒（PCV）是1974年在猪肾传代细胞系PK-15中发现的一种污染病毒,该病毒不产生细胞病变效应（CPE）,无致病性,命名为PCV—1;而后在猪断奶后多系统衰竭综合征（PMWS）中分离的猪圆环病毒,有致病性,命名为PCV-2。PMWS主要感染1～5月龄猪,发病率为4％～30％,致死率为70％～80％。断乳猪和生长猪临床表现为进行性消瘦、呼吸困难、皮肤苍白、黄疸、腹泻和体表淋巴结肿大。多种组织发生广泛的肉芽肿性炎症,肉芽肿性淋巴结炎、间质性肺炎、肝炎、间质性肾炎和胰腺炎。病理组织学变化主要是淋巴细胞组织不同程度的衰竭萎缩,淋巴细胞减少。世界许多养猪国家都发生了本病,我国也有本病流行。 相似文献
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猪断乳后多系统衰竭综合征（PMWS）是一种由猪圆环病毒2型（porcinecircovirus2，PCV-2）引起的新病。PMWS主要感染1—5月龄猪，发病率为4％-30％，致死率为70％-80％。断乳猪和生长猪临床表现为进行性消瘦、呼吸困难、皮肤苍白、黄疸、腹泻和体表淋巴结肿大。多种组织发生广泛的肉芽肿性炎症，肉芽肿性淋巴结炎、间质性肺炎、肝炎、间质性肾炎和胰腺炎。病理组织学变化主要是淋巴细胞组织不同程度的衰竭萎缩，淋巴细胞减少。世界许多养猪国家都发生了本病，我国也有流行。PMWS严重影响猪的生长发育，没有有效的治疗方法，尚无有效疫苗可供应用，因此本病的正确诊断尤为重要，现将有关进展综述如下。 相似文献
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The pathogenesis of encephalomyocarditis (EMC) due to the EMC virus (EMCV) was studied in 24 piglets oro-nasally infected with the field isolate B279/95. Two pigs were kept as negative controls and were euthanised at hour 0. The remaining 24 were euthanised every 6 h up to 78-h post infection (hpi). Virus isolation, histological examination and EMCV immunodetection were performed on the spleen, intestine, pancreas, liver, kidneys, heart, lungs, lymph nodes, tonsils and brain. EMCV was isolated at 6-hpi from the intestine and lymph nodes and at 12-hpi from the heart. From 6 to 12-hpi, scattered degenerate myocardiocytes were immunolabelled. Subsequently, myocarditis developed and progressively worsened. Immunopositive reaction in tonsil macrophages, observed in the early stage of infection (6-hpi), suggests that tonsils are the portal of entry, and by mean of wandering macrophages the EMC virus is then distributed through the body. Afterwards, EMCV-B279/95 replicates intensively in the cytoplasm of myocardiocytes and the acute myocarditis is strictly related to the tropism of these cells. Four pigs died spontaneously. In three animals no post mortem lesions or virus were isolated/detected, although all of them showed mild myocarditis. The experimental infection with EMCV B279/95 indicates: (i) the experimental protocol mimics the individual variability observed in natural disease, (ii) tonsils are the portal of entry of infection and the heart is the target organ, (iii) EMCV provides a valuable animal model for comparative studies on progressive viral myocarditis. 相似文献
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PMWS: experimental model and co-infections 总被引：12，自引：0，他引：12
Allan GM McNeilly F Ellis J Krakowka S Botner A McCullough K Nauwynck H Kennedy S Meehan B Charreyre C 《Veterinary microbiology》2004,98(2):165-168
Porcine circovirus type 2 (PCV2) is now recognised as the causal agent of porcine multisystemic wasting syndrome (PMWS), an economically important wasting disease of young pigs [J. Vet. Diagn. Invest. 12 (2000) 3]. Gross lesions of PMWS include generalised lymphadenopathy, hepatitis, nephritis and pneumonia and typical histological lesions include lymphocytic depletion and multinucleated giant cell formation in lymph nodes, degeneration and necrosis of hepatocytes, and multifocal lymphohistocytic interstitial pneumonia.This communication will review the results of experimental infections of gnotobiotic (GN), colostrum-deprived (CD) and colostrum-fed (CF) pigs within our group, and elsewhere, with PCV2 and the conclusions that can be drawn from this work. 相似文献
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A two-year-old Large White boar from a pig breeding stock in an indoor farm in Switzerland presented anorexia, reduced general condition and fever. Despite antibiotic and anti-inflammatory treatment, the boar developed severe dyspnoea and cyanosis, and died after 4 days. At necropsy, no gross lesions were observed. Histopathologically, multifocal degeneration and necrosis of myocardial fibers with interstitial edema, severe multifocal non-suppurative myocarditis and hepatitis, and non-suppurative interstitial nephritis were observed. In heart samples, groups of organisms resembling apicomplexan tachyzoites were seen associated with the lesions. A PCR using the primers COC1-COC2 that target a conserved region of the small-subunit rRNA gene of Apicomplexa was performed with DNA from paraffin-embedded tissues. An amplification product of about 350 bp was obtained from heart samples. A sequence analysis showed 100% identities with GenBank sequences reported for Sarcocystis miescheriana. The histopathological observations and molecular findings in combination with the clinical signs, and absence of other pathologic agents highly suggested that an acute infection with S. miescheriana was the cause of death in this boar. To our knowledge, this the first report of fatal acute sarcocystosis after natural infection in a pig breeding herd. 相似文献
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The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease — 2004–2006 
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下载免费PDF全文 Susy Carman Hugh Y. Cai Josepha DeLay Sameh A.Youssef Beverly J. McEwen Carl A. Gagnon Donald Tremblay Murray Hazlett Peter Lusis Jim Fairles Hazel S. Alexander Tony van Dreumel 《Canadian journal of veterinary research》2008,72(3):259-268
In the late fall of 2004 more severe lesions of porcine circovirus-2 associated disease (PCVAD) than usual occurred during an outbreak of porcine circovirus-2 (PCV-2) infection in Ontario nursery and grower/finisher pigs. The lesions were of unprecedented severity and included diffuse bronchointerstitial pneumonia, granulomatous enteritis, vasculitis, interstitial nephritis, and new lesions of splenic infarction. Some affected herds had up to 50% mortality. The outbreak correlated with the sudden emergence of a variant PCV-2, with PCR restriction fragment length polymorphism (RFLP) type 321. Phylogenetic comparison of ORF2 sequences and full genome sequences showed the new variant to be different from the previously dominant RFLP type 422 viruses, and similar to viruses that had occurred in France and other European and Asian countries. A subsequent retrospective study showed a statistically significant increase in the frequency of histological lesions in lymph node, spleen, lung, small intestine, colon and kidney, for pigs spontaneously infected with RFLP type 321, compared with the older RFLP type 422 strain. Viral burden, based on IHC staining in lymph node, also showed a statistically significant increase in pigs infected with the newer variant RFLP type 321, compared with the older RFLP type 422 strain. This enhanced virulence in pigs infected with PCV-2 RFLP type 321 strain may be related to the genetic differences in this new strain of PCV-2. This virus is now the dominant strain of PCV-2 virus found in Ontario and Quebec swine. 相似文献
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The distribution of intravenously inoculated swine hepatitis E virus (HEV) was assessed by in situ hybridisation for a period of 50 days. Evidence of apparent clinical disease was found in only one pig in the HEV infected group. The only gross lesion observed was mildly enlarged mesenteric lymph nodes at 50 days post infection (dpi). Histopathologically, mild lymphoplasmacytic infiltration and focal hepatocellular necrotic lesions were found in HEV-infected pigs. Swine HEV nucleic acids were detected by RT-PCR in the faeces at 3 dpi in 100% of the 18 pigs infected with the virus. Thereafter, the number of positives declined.The most consistent and intense signal was found in the liver of infected animals using in situ hybridisation. The positive cells were hepatocytes, Kupffer cells, bile epithelial cells and interstitial lymphocytes. Swine HEV RNA was localised in the cytoplasm of the hepatocytes, with a slightly granular pattern of staining, but hybridisation signals were not observed in degenerative or vacuolated hepatocytes. HEV was much less frequently detected in extrahepatic tissues such as lymph nodes, tonsil, spleen and small and large intestine. It was concluded that swine HEV had replicated primarily in the hepatocytes and infection resulted in subclinical infection with minimal histopathological changes in the liver. 相似文献
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Variable nature of border disease on a single farm: clinical and pathological description of affected sheep 总被引：1，自引：0，他引：1
The clinical, pathological and virological findings in cases of Border disease occurring on a farm were studied. Fourteen abnormal lambs approximately three months old were obtained and were necropsied within 18 months at death or slaughter. Another lamb born during the study to a persistently infected ewe was also examined. The findings of these 15 animals are presented in three groupings. The animals of group A were persistently viraemic and hypomyelinogenesis was present in two lambs at one and 14 weeks old. Systemic, chronic, multifocal inflammation including nephritis, myocarditis and pneumonitis was found in older sheep. Group B lambs were non-viraemic and the predominant necropsy finding was cystic cerebral cavitation of variable severity. Histological examination showed cerebellar dysplasia and in two animals the retinas also showed multifocal photoreceptor cell atrophy. Lambs in group C were also non-viraemic and although these failed to thrive, no consistent pathological changes were found. It is concluded that the dams of animals in group A were infected in early pregnancy while those of animals in groups B and C were infected at mid-gestation or later. 相似文献
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Kazacos KR Reed WM Kazacos EA Thacker HL 《Journal of the American Veterinary Medical Association》1983,183(9):967-971
Infection with Baylisascaris procyonis, the common roundworm of raccoons, was found to be the cause of an epizootic of fatal CNS disease in domestic rabbits (Oryctolagus cuniculus). Clinical signs included torticollis, ataxia, tremors, and falling. Gross lesions were limited to white, raised nodules (1 to 1.5 mm) on the epicardium, endocardium, and liver serosa, and they were found to be larval granulomas. Microscopic lesions included multifocal myocarditis, multifocal hemorrhagic tracks and associated necrosis and inflammation in the liver, multifocal eosinophilic myositis, focal nephritis, and mild interstitial pneumonia; larval granulomas were seen in the heart, liver, lung, and mandibular salivary gland. Lesions in the brain consisted of multifocal areas of necrosis and inflammation in the cerebrum, cerebellum, midbrain, and medulla, with accompanying perivascular leukocyte aggregates and neuronal and axonal degeneration. Large numbers of large ascarid larvae were seen in the brain. Epidemiologically, infection was linked to the use of contaminated straw from a barn used by raccoons. On the basis of this study as well as that of similar studies, it was concluded that barn use and contamination by raccoons may be an important source of infection of animals and possibly man with this parasite. 相似文献
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Muco-cutaneous candidiasis in two pigs with postweaning multisystemic wasting syndrome 总被引：1，自引：0，他引：1
Zlotowski P Rozza DB Pescador CA Barcellos DE Ferreiro L Sanches EM Driemeier D 《Veterinary journal (London, England : 1997)》2006,171(3):566-569
In two distinct commercial swine herds, poor weight gain and an increased number of animals showing wasting were observed among nursery and growing pigs. Cases of postweaning multisystemic wasting syndrome (PMWS) and infection with Haemophilus parasuis had been previously diagnosed in these herds. One growing wasted pig from each herd was necropsied and showed enlarged lymph nodes. Pseudomembranous material adhered to the dorsum of the tongue, soft and hard palate in case 1, and in case 2, fibrinous material was seen as whitish plaques on the oesophageal surface with hyperkeratosis of the non-glandular stomach. The main histological lesions in both cases were found in lymphoid tissues with a multifocal accentuated lymphohistiocytic infiltrate, areas of lymphoid depletion and intracytoplasmic inclusions in histiocytic cells in lymph nodes and Payer's patches. Focally, extensive ulceration was found in the stratified pavement epithelium of the tongue with necrosis and necrosuppurative infiltrate in case 1; in case 2, there was ulceration in the stomach with lymphohistiocytic infiltrate in the submucosa and ulceration in the mucosa of the oesophagus associated with yeast cells and pseudo-hyphae. Candida albicans was isolated from the oral cavity lesions. Immunohistochemistry of the lymph nodes was positive for porcine circovirus 2 (PCV2). The association between PMWS and mucocutaneous candidiasis reported here supports the potential immunosuppressive state of PMWS infected pigs. 相似文献
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Six 8-week-old ascarid-naive pigs which were experimentally infected with 72,000 embryonated Baylisascaris procyonis eggs of raccoon origin developed lesions limited to the intestines and liver. Intestinal lesions consisted of multifocal areas of inflammation by macrophages, eosinophils, and lymphocytes in the mucosa and submucosa, in association with Baylisascaris larvae; similar lesions were seen in the mesenteric lymph nodes. Typical white, granulation type, multifocal interstitial hepatitis ("milk-spots"), 1 to 5 mm in diameter, were seen in the livers by 7 days, with resolution by 47 days. Microscopically, these consisted of multifocal areas of marked periportal and interlobular edema, and influx of eosinophils, and large intralobular aggregates of eosinophils. At 47 days, hundreds to thousands of small white granulomas were seen on the serosa of the intestines; microscopically, they were discrete collections of macrophages, lymphocytes, and eosinophils in the submucosa and muscle layers surrounding nonviable remnants of Baylisascaris larvae. Larvae or lesions were not seen in other tissues, including the brain. These experiments indicated that B procyonis will undergo limited migration in swine and can produce typical white spots in the liver. The larvae were killed by cellular reactions in the intestinal wall and liver, and, unlike the situation in most other animals infected with this parasite, no somatic migration or CNS disease occurred after infection. 相似文献