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1.
采用3种限制性内切核酸酶对杜洛克、内江猪和荣昌猪共153头的钙蛋白酶抑制蛋白(CAST)基因进行PCR-RFLP分析。结果表明:①HinfI、MspI和RsaI均检测到多态性,同时还发现一个杜洛克个体的HinfI-RFLP出现120bp突变片段。②X2检验表明,HinfI、MspI和RsaI酶切所产生的基因及基因类型处于Hardy-Weinberg平衡状态(p<0.05);杜洛克、内江猪和荣昌猪三个品种猪的优势基因型分别为ABCCEE、AACCEE、AACCFF,在99%程度上分别与相应的品种有关。  相似文献   

2.
猪H-FABP基因多态性及其与肌内脂肪含量的相关研究   总被引：3，自引：0，他引：3  
旨在研究猪H-FABP基因的遗传多态性及其与肌内脂肪含量的遗传效应。利用PCR-RFLP(HinfⅠ、MspⅠ、HaeⅢ、Hinf*Ⅰ4种限制性内切酶)分子标记技术检测了中国地方猪种雅南猪、大河猪,培育品种大河乌猪以及杜洛克与长白和约克三元杂交商业群体共148头猪心脏型脂肪酸结合蛋白(H-FABP)基因5′-上游区和第二内含子的遗传变异,并利用固定效应模型分析了H-FABP基因在杜洛克与长白和约克三元杂交商业群体中对肌内脂肪含量的遗传效应。结果表明:(1)在HinfⅠ和MspⅠ位点上,所有4个猪群都存在多态;在HaeⅢ位点上,除雅南猪只出现单态外,其余3个猪群都出现多态;在Hinf*Ⅰ位点上,除杜洛克与长白和约克三元杂交商业群体出现多态外,其余3个猪群只表现单态。(2)4个位点对肌内脂肪含量的影响差异显著,各基因型肌内脂肪含量最小二乘均值关系即HhHH,bbBbBB,AaAA,DDDddd。结合H-FABP基因的生理功能和已有的研究结果来看,可在特定的群体中将其作为影响猪肌内脂肪含量的候选基因。  相似文献   

3.
采用PCR-RFLP方法对72头苏太猪的钙蛋白酶抑制蛋白(calpastatin,CAST)基因遗传多态性与肉质性状的相关性分析。对CAST基因第6内含子上MspⅠ、HinfⅠ、RsaⅠ三种酶切位点进行检测,结果表明:CAST基因第6内含子上,MspⅠ酶切位点的3种基因型间在肌肉的嫩度、pH值、系水力水平指标上均差异显著(P0.05);HinfⅠ酶切位点的3种基因型间在肌内脂含量指标上差异显著(P0.05),在肌肉嫩度上差异极显著(P0.01)。RsaⅠ酶切位点上也发现了3种基因型,但各基因型的不同性状间差异不显著(P0.05)。  相似文献   

4.
采用3种限制性内切核酸酶对杜洛克、内江猪和荣昌猪共153头的钙蛋白酶抑制蛋白(CAST)基因进行PCR-RFLP分析。结果表明:HinfI、MspI和RsaI均检测到多态性,同时还发现一个杜洛克个体的HinfI-RFLP出现120bp和80bp突变片段。χ2检验表明:HinfI、MspI和RsaI酶切产生基因已经达到Hardy-WeLnberg平衡状态(p<0.05);数量上三个品种的优势基因型ABCCEE、AACCEE、AACCFF,在99﹪程度上分别与相应的品种有关。  相似文献   

5.
为探索H-FABP和LPIN1基因在不同猪种中的多态性分布情况,应用PCR-RFLP技术检测了61头军牧1号白猪、51头杜洛克猪和51头藏猪H-FABP和LPIN1基因的多态性分布。结果发现,军牧1号白猪、杜洛克猪和藏猪在H-FABP基因的Hinf Ⅰ多态性位点上均表现为单一的HH型,而Hea Ⅲ多态性位点上均表现出多态性,等位基因D的基因频率分别为0.1475、0.2255和0.7647,Msp Ⅰ多态位点上,军牧1号白猪表现为单一的aa型,杜洛克猪和藏猪则表现为多态性,等位基因A的基因频率分别为0.6078和0.4609。在LPIN1基因的Eco88 Ⅰ多态性位点上,杜洛克猪表现为单一的AA型,军牧1号白猪和藏猪表现为多态性,等位基因A的基因频率分别为0.4262和0.2059;在Bsh1236 Ⅰ多态性位点上,杜洛克猪表现为单一的TT型,军牧1号白猪和藏猪则表现为多态性,等位基因T的基因频率分别为0.7459和0.2059。  相似文献   

6.
选取了106头莱芜猪核心群及部分后备猪,用PCR-RFLP的方法分别检测了酰基辅酶A合成酶长链4（Acly-CoA synthetase long chain family 4,ACSL4）基因的SNP G2645A位点和心脏型脂肪酸结合蛋白（Heart fatty acid-binding protein,H-FABP）基因的HinfⅠ及MspⅠ位点的多态性。结果显示,在ACSL4的SNP G2645A位点共检测到AG和GG 2种基因型,没有检测到AA基因型;G等位基因是优势等位基因;莱芜猪该位点呈高度多态。在H-FABP的HinfⅠ位点共检测到HH、Hh和hh 3种基因型,莱芜猪在该位点呈中度多态。莱芜猪在上述2个位点均处于Hardy-Weinberg平衡状态。经测序验证,莱芜猪H-FABP基因HinfⅠ位点的多态性是1 324位点T的插入、缺失所造成的。莱芜猪在H-FABP的MspⅠ位点没有多态性。在莱芜猪本品种选育中,可以顺序选择ACSL4G2645A位点GG基因型纯合个体和H-FABP HinfⅠ位点HH基因型纯合个体,提高莱芜猪IMF性状的均一性,将进一步促进莱芜猪种质的保护和利用。  相似文献   

7.
应用PCR-RFLP方法对金华猪Ⅰ系(164头)、金华猪Ⅱ系(41头)和金华猪Ⅲ系(52头)的心脏脂肪酸结合蛋白(H-FABP)基因的1125～1817bp和1401～2216bp段进行扩增,并用HinfⅠ、HaeⅢ和MspⅠ酶分别酶切,结果表明只有HinfⅠ存在RFLP。分析不同基因型对金华猪死产数和母仔猪初生重的影响,结果表明,H-FABP基因对经产母猪的死产数影响接近极显著(P=0.015),对头胎母仔猪初生重的影响不显著(P>0.05),对经产及所有胎次的母仔猪初生重影响极显著(P<0.01)。  相似文献   

8.
采用PCR-RFLP法对甘孜藏猪、合作猪、滇南小耳猪、荷包猪、五指山猪和巴马小型猪SLA-DQB基因外显子2多态性进行分析。结果表明:经RsaⅠ酶切检测到5种等位基因,14种RFLP带型,遗传杂合度(He)在0.538~0.729,多态信息含量(PIC)在0.488~0.728,该位点表现为高度多态;χ~2适合性检验显示,甘孜藏猪、巴马小型猪和五指山猪3个群体处于Hardy-Weinberg平衡状态。经HinfⅠ酶切检测到3种等位基因,6种RFLP带型,He在0.315~0.524,PIC在0.291~0.461,在该位点表现为中度多态。χ~2适合性检验显示,甘孜藏猪、滇南小耳猪、巴马小型猪、荷包猪4个群体处于Hardy-Weinberg平衡状态。因此,6个小型猪种的SLA-DQB基因外显子2多态性丰富且存在明显差异。  相似文献   

9.
利用PCR-RFLP、Forced-PCR-RFLP、DNA测序法研究中国西农萨能奶山羊(Sa)、关中奶山羊(GZ)和内蒙古白绒山羊(IMWC)3个品种LHX3基因的分子遗传特性.结果表明,在第2内含子452 nt处存在T＞C的突变,第6外显子352 nt处存在T＞C的突变.在Hinf Ⅰ位点,GZ、Sa和IMWC的等位基因T/C频率分别为0.492 9/0.507 1,0.376 3/0.623 7,0.698 4/0.301 6;在Msp Ⅰ位点,GZ、Sa和IMWC的等位基因T/C频率分别为0.492 9/0.507 1,0.633 8/0.366 2,0.267 2/0.732 8;以上位点在不同品种中的多态信息含量(PIC)在0.314 9～0.374 9之间.独立X2分析表明,Hinf Ⅰ和Msp Ⅰ位点的基因型频率分布在不同山羊品种间均存在极显著差异,暗示品种因素对这些基因型的分布具有显著影响.  相似文献   

10.
9个猪种H-FABP基因5''-上游区和第二内含子的遗传变异   总被引：17，自引：5，他引：17  
以 8个中国地方猪种和引进品种大白猪共 40 9头猪为实验动物 ,利用PCR RFLP技术研究了H FABP基因的 5’ 上游区和第二内含子内的遗传变异。结果在 9个品种的 5’ 上游区中都验证了一个HinfⅠ RFLP ,变异的酶切位点在 132 2位 ,等位基因H ,在皖南花猪 ,二花脸猪 ,金华猪 ,小梅山猪 ,滇南小耳猪 ,香猪 ,荣昌猪 ,北京黑猪和大白猪中的频率分别为 0 36 ,0 34 ,0 6 0 ,0 6 6 ,0 80 ,0 73,0 76 ,0 70和 0 90。大白猪和滇南小耳猪的HinfⅠ RFLP的基因型分布相似 ,二者分别与其它 7个猪种之间存在着极显著的差异 ;皖南花猪和二花脸猪相近 ,二者与另外 7个品种之间的差异也达到极显著水平。在第二内含子中 ,验证了一个HaeⅢ RFLP ,该多态性只出现在皖南花猪和大白猪中 ,等位基因D的频率分别为 0 99和 0 6 1。另外 ,发现一个新的多态HinfⅠ 酶切位点 ,测序结果表明 ,该多态位点的产生是由于 1970位碱基C突变为T造成的。该多态位点只在皖南花猪和大白猪中出现 ,等位基因B的频率在两品种中分别为 0 86和 0 5 8。在所有测定的猪种中 ,都没有发现MspⅠ RFLP。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

16.
Comparison of different tests to diagnose feline infectious peritonitis   总被引：1，自引：0，他引：1  
  相似文献   

17.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

20.
Tropical Animal Health and Production -  相似文献   

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