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化学杀雄剂SQ-1诱导青海高原春小麦雄性不育的初步研究 总被引:3,自引:0,他引:3
为了研究化学杀雄剂SQ-1对青海高原春麦区的杀雄效果及不同春小麦品种对SQ-i反应的遗传差异,采用时期为主区、剂量为副区、小麦品种为亚副区的三因素裂区设计方法,对化学杀雄剂SQ-1诱导青海高原春小麦雄性不育的效果进行了研究.结果表明,在Feekes 8.0~Feekes 8.5时期,5.0 kg/hm2剂量下,参试品种均能被诱导产生大于95%的雄性不育率.对参试品种进行人工饱和授粉,其结实率均能达到90%以上,证明SQ-1对雌蕊无明显影响. 相似文献
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通过使用化学杂交剂SQ-1来诱导小麦雄性不育,是当今小麦杂交育种界普遍使用的方法。本文以西农1376为研究对象,通过测定喷施化学杂交剂SQ-1后的小麦旗叶叶绿素A、B及其总量的变化,探讨两者之间的关系,探究化学杂交剂SQ-1的雄性不育机理是否与叶片有关。结果表明,经过SQ-1处理的植株叶绿素含量均低于对照植株,而处理植株之间随着SQ-1浓度的增加,叶绿素含量逐渐降低,说明化学杂交剂SQ-1对小麦旗叶确实存在一定的影响,随着剂量的增加,破坏细胞膜的程度也相应加大,对小麦叶片的伤害程度也增加。 相似文献
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GAPDH基因表达与小麦生理型雄性不育花药败育的关系 总被引:3,自引:0,他引:3
为分析三磷酸-甘油醛脱氢酶(GAPDH)基因与小麦(Triticum aestivum)生理型雄性不育花药败育的关系,本研究以新型小麦化学杀雄剂SQ-1作为诱导剂,普通小麦西农1376为材料,构建了西农1376不育和可育等生理系;采用半定量逆转录聚合酶链式反应(RT-PCR)技术分析了不育和可育等生理系不同发育时期花药中GAPDH基因的表达模式.结果表明,该基因在正常小麦花粉发育的单核期、二核期和三核期,表达丰度比较一致,没有明显变化,但在化学杀雄剂SQ-1诱导的生理型雄性不育花药中,不同发育期表达量存在显著差异,单核期表达最弱,二核期最高.与同期正常花药相比,GAPDH基因在生理型雄性不育花药三个发育期均呈下调表达,其中在大量花粉粒败育的单核期,表达量下调最显著,其次为三核期和二核期,证明化学杀雄剂SQ-1对GAPDH基因的表达具有明显抑制作用.因此,化学杀雄剂SQ-1诱导小麦生理型雄性不育形成过程中,可能与GAPDH基因表达减少导致细胞中能量供给不足有一定关系. 相似文献
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氨基酸合成抑制剂类除草剂诱导油菜雄性不育效果评价 总被引:1,自引:0,他引:1
甘蓝型油菜对以乙酰乳酸合酶为靶标的磺酰脲类等除草剂很敏感, 其中部分除草剂具有很强的化学杀雄作用。本文通过油菜抽薹期叶面喷施试验, 比较26种能够抑制氨基酸生物合成的除草剂及复配剂对甘蓝型油菜雄蕊育性的影响。结果显示除过双草醚、麦喜、胺苯磺隆外, 其余23种除草剂均对油菜具有不同程度杀雄作用。其中600 mg hm–2的咪唑乙烟酸、150mg hm–2的吡嘧磺隆、240 mg hm–2的烟嘧磺隆、200 mg hm–2的单嘧磺隆和120 mg hm–2的氯磺隆具有较高杀雄率, 但容易产生药害。而60~90 mg hm–2的酰嘧磺隆、苯磺隆及其复配剂对油菜杀雄率大于95%, 持续时间长, 对雌蕊结实性能影响较小, 且在20个甘蓝型油菜品种(系)上杀雄效果稳定, 可以作为油菜等植物的最佳化学杀雄剂活性成分。本试验证明可以从氨基酸合成抑制型除草剂中筛选化学杂交剂, 为进一步开发化学杀雄剂提供了参考。 相似文献
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介绍了运用冷冻干燥技术进行速溶蛋花汤的加工,研究了生产工艺、特点以及影响产品质量的主要因素。试验表明,采用冷冻干燥技术加工的速溶蛋花汤,具有营养丰富、风味独特、方便快捷等特点。 相似文献
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DENG Zhao-jing 《保鲜与加工》2005,(1):153-156
A new definition of inertia,i.e. the momentum is the measuring of inertia of matter, is proposed. The article through and etc., subjects, the correctness of the new notion of inertia:The monentum is the measuring of inertia has been demonstrated, and the unsolved contradictions of the traditional definition of inetia. And the mass is the measuring of inertia has been revealed. 相似文献
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香蕉是热带亚热带发展中国家重要的粮食作物和碳水化合物来源。但近年来,香蕉生产受到严重的病虫危害。大多数香蕉栽培品种是三倍体,生长周期长,而且不孕。由于没有种子,给繁殖和育种带来一定的困难。遗传转化技术的发展为香蕉品种的改良提供了一种有效的手段。香蕉的遗传转化方法有电激法、基因枪法、农杆菌介导法等。农杆菌介导法的应用是香蕉品种改良的一个重大突破。香蕉遗传转化的外植体也发展到多种,有原生质体,胚性细胞悬浮系,分生组织,以及横切薄片等。近几年,随着分子生物学的发展,出现了转化效率更高,重复性更好的香蕉遗传转化技术。如农杆菌和基因枪结合法,离心辅助农杆菌介导法、真空渗透技术等。这些新技术新方法的出现,必将推动香蕉产业高速发展。 相似文献
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Automobile comfort dynamic simulation is necessary when we predict and evaluate the comfort of a automobile or optimize performance of automobile. The nine DOF vibration model of automobile is established , which acts root mean square value of body in multiple work conditions as comfort evaluation indices. Based on the comfort simulation, the Hongyan 1160 is predicted in the way of comfort. The programmes can be used in many different automobiles . The computational result indicates that the model can simulate vibration of automobile truly. It is significant to research automobile comfort evaluation to optimize performance of automobile and comfort simulation .The computational program can be applied to optimize or simulate which has some reference value. 相似文献
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南瓜降糖功能及其系列食品加工技术 总被引:3,自引:0,他引:3
阐述了南瓜在糖尿病辅助治疗方面的功能特性,介绍了南瓜降糖主要制品南瓜全粉、南瓜脆片、南瓜果酱和南瓜肉汁的工艺流程和操作要点。 相似文献
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母猪繁殖障碍成因分析 总被引:3,自引:0,他引:3
繁殖障碍是导致母猪淘汰的最主要因素,直接造成母猪非生产天数延长,繁殖猪群生产效率降低,严重影响猪场的经济效益。本文作者对引起母猪繁殖障碍的多方面因素,包括营养因素(如饲养水平、饲料品质、断奶日龄)、环境因素(如温度、湿度、通风、光照)、疫病因素、生产技术及管理因素进行了详细阐述。 相似文献
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Applicability of molecular markers in the context of protection of new varieties of cucumber 总被引:2,自引:0,他引:2
G. P. Bernet S. Bramardi D. Calvache E. A. Carbonell M. J. Asins 《Plant Breeding》2003,122(2):146-152
The growing number of candidate varieties presented every year at each national Plant Variety Protection Office and their decreasing genetic variability forces strategies to be adopted that will reduce costs without losing rigour when deciding about the acceptance or rejection of a candidate variety. Molecular markers have been envisaged as a reliable tool to establish differences, but can molecular markers be used for assessing distinctness? A comparison between a molecular and a morphological characterization of 41 seed samples belonging to 36 cucumber varieties and including several external controls has been carried out to investigate the applicability of molecular markers in the context of distinctness, uniformity and stability (DUS) tests and the protection of new varieties. Both types of character indicate the same relationships among main groups, Dutch and Beth‐Alpha types, and Gerking and Slice types, the correlation between distance matrices being only 0.6489. Varieties considered uniform for morphological traits proved not to be so for molecular markers. Therefore, molecular characterization is not offering the same estimates of uniformity and relatedness between varieties as does morphological characterization. External controls were used to establish distinction limits with morphological and molecular dendrograms to focus on those varieties, which were suspiciously similar. The results with cucumber show that molecular identity could be used to assess any lack of distinctness and so corroborate the morphological assessment of candidate varieties. The morphological trait ‘type’ provides distinct characterization of varieties and the molecular characterization of candidate varieties could be used to design better field experiments to assess distinction within each group of morphological cucumber type. 相似文献