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1.
《中国畜牧杂志》2016,(21)
为了探讨小卵泡液、放线菌酮(CHX)对卵母细胞预成熟、囊胚滋养层细胞囊泡(TVS来源于体外受精培养14 d的滋养层细胞)、维生素对牛体外胚胎质量的影响。在B超仪下进行牛活体取卵(OPU),从牛活体卵巢采集卵母细胞,进行体外成熟、体外受精、早期胚胎体外培养。结果表明:10%小卵泡液及8%CHX的卵母细胞预成熟4 h组显著优于对照组;在体外受精及早期胚胎培养液CR1aa中添加10μg/m L维生素C组与TVS共培养组的卵裂率和囊胚发育率均高于其他试验组(P0.05);体外胚胎与TVS共移植受胎率显著高于对照组(P0.05)。说明活体采集的卵母细胞经体外预成熟处理,可以达到核质同期化的目的,早期胚胎的培育过程中加入TVS、抗氧化剂等可以克服早期胚胎的发育阻滞,提高体外胚胎的囊胚发育率。 相似文献
2.
将收集的猪COCs置于添加不同浓度(0.0,0.3,0.6,1.2μmol/L)甘草酸单铵盐(monoammonium glycyrrhizinate, MAG)的卵母细胞体外成熟培养液中培养46 h,统计成熟率，通过免疫荧光染色检测成熟卵母细胞ROS表达水平；对成熟卵母细胞体外受精，于胚胎培养液内体外培养48,120 h,分别统计体外受精胚胎卵裂率、囊胚率，并用Hochest荧光染色检测囊胚总细胞数；结合成熟率及IVF胚胎发育囊胚率选定最佳添加浓度，在体外成熟培养液中添加最适浓度MAG,以0μmol/L MAG为对照组，体外培养46 h后，利用免疫荧光染色检测猪成熟卵母细胞的线粒体膜电位水平和细胞凋亡水平。结果显示，与对照组相比，不同浓度MAG处理组猪卵母细胞体外成熟率均有所提高，但差异均不显著(P>0.05);不同浓度MAG添加均可降低猪卵母细胞ROS水平，与对照组相比，0.3,0.6μmol/L组差异显著(P<0.05),1.2μmol/L组差异极显著(P<0.01)。0.3μmol/L添加组显著提高了猪体外受精囊胚率(P<0.05),但不同MAG添加组对... 相似文献
3.
Ghrelin对水牛体外受精和孤雌激活胚胎体外发育的影响 总被引：1，自引：0，他引：1
本研究的目的是探讨Ghrelin对水牛体外受精和孤雌激活胚胎体外发育的影响.体外成熟的水牛卵母细胞经体外受精或离子霉素孤雌激活后.分别在舍0,0.5,5,50和500 μg/L Ghrelin的培养液中进行体外培养,观察各组胚胎的卵裂率和囊胚率.结果显示,在培养液中添加不同浓度的Ghrelin对体外受精和孤雌激活胚胎的卵裂率均无显著影响(P>0.05),但添加500 μg/L的Ghrelin显著提高体外受精胚胎的囊胚发育率(33.5% vs 13.7%,P<0.05),50 μg/L或500 μg/L的Ghrelin均显著提高孤雌激活胚胎的囊胚发育率(32.4%和34.6% vs 14.5%,P<0.05).结果表明,培养液中添加Ghrelin对胚胎的早期卵裂没有影响,但可促进水牛体外受精和孤雌激活胚胎囊胚的形成. 相似文献
4.
为研究表皮生长因子(EGF)、β-巯基乙醇(β-ME)、亚硫磺酸(HTAU)对牛卵母细胞体外成熟(IVM)及孤雌激活胚胎体外发育(IVC)效果的影响,实验采集牛卵巢采用切割法收集卵丘-卵母细胞复合体(COCs)随机处于不同浓度EGF、β-ME培养液成熟培养24 h后孤雌激活,研究其在不同胚胎培养液中的后续发育,以期筛选出最好的牛卵母细胞IVM-IVC条件。结果表明:添加25、50、100 ng/mL EGF组的成熟率、卵裂率均高于对照组,其中50 ng/mL EGF组的成熟率、卵裂率与对照组差异显著(P<0.05);50、100、500μmol/L的β-ME对牛卵母细胞体外成熟没有促进作用;50 ng/mL EGF与50、100μmol/Lβ-ME组合并无协同作用;胚胎培养基中添加不同浓度EGF对早期胚胎的体外发育无显著影响;而添加100μmol/Lβ-ME组的囊胚率、囊胚细胞数均极显著高于对照组(P<0.01);在成熟液中添加50 ng/mL EGF和100μmol/Lβ-ME、胚胎培养液中添加0.5 mmol/L HTAU孤雌胚发育最好。 相似文献
5.
为建立稳定高效的活体采卵-体外受精技术体系,提高体外胚胎生产效率,本研究先利用屠宰场采集的新鲜卵巢卵母细胞进行体外受精,通过胚胎发育潜力来筛选最佳的体外胚胎培养液;再进一步研究不同种公牛精液和供卵母牛对活体采卵-体外受精效率的影响。结果显示,CR1aa培养液和mCR1aa培养液卵裂率差异不显著（P>0.05）,但mCR1aa组的囊胚发育率显著提高（28.1% vs 20.6%,P<0.05）;选取的3头荷斯坦种公牛精液的活体采卵-体外受精胚胎的卵裂率差异不显著（P>0.05）,但1号种公牛精液体外受精后囊胚率（38.7%）显著高于2号和3号（23.8%&22.9%）（P<0.05）;随机选择的3头活体采卵供体母牛（H1、H2、H3）获得的头均可用卵母细胞数无显著差异,但H1和H2供体母牛体外受精胚胎的卵裂率和囊胚率均显著高于H3供体牛（P<0.05）,且H1供体牛体外受精囊胚率显著高于H2供体牛（P<0.05）。结果表明,mCR1aa培养液能显著提高体外受精囊胚发育率,适用于体外胚胎生产;种公牛精液和供体母牛个体差异会直接影响活体采卵-体外受精胚胎的生产效率,为奶牛活体采卵-体外受精生产技术体系的优化提供参考。 相似文献
6.
试验旨在研究不同种类、不同浓度的糖对牦牛卵母细胞体外成熟和发育能力的影响,进一步探索和优化牦牛卵母细胞培养体系,提高卵母细胞体外成熟和胚胎生产效率。在牦牛卵母细胞成熟液中添加不同浓度（0、5和10 mmol/L）的葡萄糖或蔗糖,培养24 h或预培养2 h后移入无糖培养基中继续培养22 h,统计卵母细胞体外成熟率及体外受精（IVF）后的胚胎卵裂率和囊胚率。结果显示,与对照组（0 mmol/L）相比,5和10 mmol/L葡萄糖组牦牛卵母细胞核成熟率和体外受精胚胎卵裂率均显著提高（P<0.05）,10 mmol/L葡萄糖组的囊胚率最高,且与对照组相比差异显著（P<0.05）。添加10 mmol/L蔗糖可以显著提高牦牛卵母细胞核成熟率（P<0.05）,但胚胎囊胚率与对照组相比差异不显著（P>0.05）。此外,用10 mmol/L葡萄糖预处理牦牛卵母细胞后其核成熟率、胚胎卵裂率和囊胚率最高,且均显著高于对照组（P<0.05）。由此可见,糖对牦牛卵母细胞体外成熟和发育有一定的影响,在成熟过程中添加适当浓度的糖能提高卵母细胞成熟率及体外受精胚胎发育能力。 相似文献
7.
利用Leptin和ITS促进体外成熟和体外培养的牛卵母细胞的发育和质量,探讨提高胚胎体外生产的质量和数量的方法和技术。试验1：体外受精胚胎的培养液：添加BSA的KSOM中添加10mL/L浓度的ITS,结果使胚胎的桑椹胚率和囊胚率显著（P〈0.05）高于培养液中不加ITS的对照组（桑椹胚率：43.48%vs29.07%,囊胚率：22.83%vs11.63%）,卵裂率、正常分裂率和8细胞率与对照组差异不显著（P〉0.05）。试验2：在卵母细胞的体外成熟液中添加10μg/L具有生物学活性的重组鸡Leptin成熟肽融合蛋白,Leptin处理组和对照组卵母细胞经体外成熟、受精后转入加有10mL/LITS的KSOM培养液进行体外培养。试验组卵裂率和正常分裂率极显著（P〈0.01）高于对照组（88.96%vs66.81%和61.11%vs29.36%）,8细胞率显著（P〈0.05）高于对照组（84.84%vs69.57%）。Leptin处理的卵母细胞在受精后的桑椹胚率和囊胚率与对照组差异不显著,但最后的囊胚数量较对照组增加1倍多,分别为IVF卵母细胞总数的14.8%和6.4%（P〈0.01）。这说明,添加Leptin对牛卵母细胞体外成熟有促进作用,可显著提高卵母细胞受精后早期胚胎的卵裂率、正常分裂率和8细胞率;加入ITS则能提高桑椹胚率和囊胚率;而Leptin和ITS的按顺序结合使用,则能大大增加体外生产胚胎的桑椹胚和囊胚的数量,从而提高胚胎体外生产的效率。 相似文献
8.
实验通过外源性添加不同浓度的卵泡抑素(FST),研究FST对牛卵母细胞体外成熟和受精卵体外发育的影响。结果表明:在以TCM199为基础培养液的卵母细胞培养体系中添加5、10、20 ng/mL FST对卵母细胞的成熟率无显著影响(P>0.05),但10 ng/mL FST可显著降低囊胚胎率7.5%、5.7%、4.6%(P<0.05)。在共培养体系下的胚胎培养液中添加10、20 ng/mL FST,受精卵的30 h卵裂率和囊胚率显著高于对照组(P<0.05);5、10、20 ng/mL的FST均可以显著提高受精卵的总卵裂率(P<0.05)。 相似文献
9.
从屠宰场收集黄牛卵巢,取皮质深层卵母细胞进行体外成熟、体外受精和早期胚胎体外培养,分析了影响其效果的因素。结果表明,在成熟培养液中添加FSH(10IU/mL)、HCG(20IU/mL)和17β-E2(1mg/L)对卵母细胞受精后早期胚胎发育能力有极显著促进作用;等量牛卵泡液(BFF)与新生牛血清(NCS)对体外受精胚胎发育效果影响不显著,以15?F为宜;颗粒细胞与输卵管上皮细胞均能显著提高卵母细胞体外成熟受精后早期胚胎的发育率,颗粒细胞 输卵管上皮细胞对克服胚胎阻滞现象效果显著。 相似文献
10.
【目的】研究柠檬苦素(limonin,Lim)对小鼠卵母细胞体外成熟(IVM)及后续体外受精(IVF)胚胎发育潜能的影响,旨在为体外成熟培养系统的优化提供参考。【方法】在小鼠体外成熟培养液中添加不同浓度的Lim(0、10、20、50 μmol/L),成熟培养12 h后统计小鼠卵母细胞第一极体(PBI)排出率,筛选体外成熟培养液中添加Lim的最适浓度;在体外成熟培养液中添加最适浓度的Lim,以0 μmol/L Lim为对照组,成熟培养12 h,通过免疫荧光染色检测活性氧(ROS)、谷胱甘肽(GSH)以及线粒体膜电位(MMP)水平;通过实时荧光定量PCR检测卵母细胞抗氧化及凋亡相关基因的mRNA表达水平。将最适Lim组及对照组卵母细胞体外成熟24 h后进行体外受精,于体外受精24 h和3.5 d分别统计胚胎卵裂率和囊胚率,并用Fluorescein-dUTP和Hoechst 33342染色分别检测囊胚总细胞数及囊胚内凋亡细胞比率。【结果】与0 μmol/L Lim组相比,20 μmol/L Lim组小鼠卵母细胞PBI排出率显著升高(P＜0.05),后续试验均用20 μmol/L Lim进行处理。与对照组组相比,20 μmol/L Lim组小鼠卵母细胞内ROS水平显著降低(P＜0.05),GSH、MMP水平均显著增加(P＜0.05),抗氧化相关基因(GPx3、CAT和Prdx3)、抗凋亡相关基因(Bcl-2、Bcl-xl)表达水平均显著上调(P＜0.05),促凋亡相关基因(Caspase-3)表达水平显著下调(P＜0.05);体外受精胚胎的卵裂率、囊胚率、囊胚总细胞数均显著增加(P＜0.05),囊胚内细胞凋亡比率显著下降(P＜0.05)。【结论】在体外成熟培养液中添加20 μmol/L Lim可以通过抑制氧化应激和细胞凋亡、增加MMP水平提高小鼠卵母细胞质量,从而提高体外受精胚胎的发育潜力。 相似文献
11.
Gao G Yi J Zhang M Xiong J Geng L Mu C Yang L 《The Journal of reproduction and development》2007,53(4):777-784
The aim of this study was to investigate the effects of iron and copper on bovine oocyte maturation, preimplantation embryo development and apoptosis of blastocysts. The concentrations of iron in the culture media were 0 (control), 0.45, 0.81, 1.96 and 3.26 mg/l, and the concentrations of copper were 0 (control), 0.093, 0.27, 0.46 and 0.68 mg/l. The changes in the iron (1.96 mg/l) and copper concentrations (0.46 mg/l) in the culture media were measured after oocyte maturation for 22 h and after zygote culture for 48, 96, 144 and 192 h. The results showed that there were no significant differences in oocyte maturation and cleavage between media containing iron and the control, but the media containing iron had higher (P>0.05) rates of 8-cell embryos, morulae, and blastocysts than the control, and addition of 1.96 mg/l of iron increased the blastocyst rate (P>0.05). The effects of copper on oocyte maturation and cleavage were similar to iron, and addition of 0.46 and 0.68 mg/l of copper increased the rates of morulae and blastocysts (P>0.05). Addition of iron or copper significantly decreased the number of apoptotic blastomeres compared with the control (P>0.05). After oocyte maturation for 22 h and zygote culture for 48 h, the iron concentrations decreased by 3.6 and 9.2%, respectively, and the copper concentrations decreased by 6.5 and 10.9%, respectively. After zygote culture for 96, 144 and 192 h, the iron concentrations decreased by 21.4, 25.5 and 27.0%, respectively, the copper concentrations decreased by 23.9, 28.3 and 30.4%, respectively. In conclusion, iron and copper played an important role in the success of culture of 8-cell embryos, morulae, and blastocysts, and long-term lack of iron or copper increased the number of apoptotic blastomeres. Furthermore, transition of primary demand for trace amounts of iron or copper from the cytoplast to culture medium for utilization by zygotes may occur after in vitro zygote culture for 48 h. 相似文献
12.
Teewara Phongnimitr Yuanyuan Liang Kanokwan Srirattana Kanchana Panyawai Nucharin Sripunya Chatchai Treetampinich Rangsun Parnpai 《Animal Science Journal》2013,84(11):719-725
In this study, the effects of the addition of L‐carnitine in in vitro maturation (IVM) medium for bovine oocytes on their nuclear maturation and cryopreservation were investigated; they were matured in IVM medium supplemented with 0.0, 0.3, 0.6 and 1.2 mg/mL of L‐carnitine (control, 0.3, 0.6 and 1.2 groups, respectively) and some of them were vitrified by Cryotop. Moreover, the effects of L‐carnitine during in vitro fertilization (IVF) and in vitro culture (IVC) on the developmental potential and quality of IVF embryos were also examined. A significantly higher maturation rate of oocytes was obtained for 0.3 and 0.6 mg/mL groups compared with the control (P < 0.05). The blastocyst formation rate in the 0.6 group was significantly improved, whereas the rate in the 1.2 group was significantly decreased when compared with the control group (P < 0.05). No significant difference was found in embryo development between the control and the L‐carnitine group after oocyte vitrification. Supplementation of IVF and IVC media with L‐carnitine had no effect on development to the blastocyst stage of IVM oocytes treated with 0.6 mg/mL L‐carnitine. In conclusion, the supplementation of L‐carnitine during IVM of bovine oocytes improved their nuclear maturation and subsequent embryo development after IVF, but when they were vitrified the improving effects were neutralized. 相似文献
13.
试验旨在探究高浓度葡萄糖对猪卵母细胞体外成熟及早期胚胎发育能力的影响。取体外分离处于生发泡期的猪卵丘卵母细胞复合体(COCs),分为3个处理组。分别用含葡萄糖浓度为5.6 mmol/L(C组)、10 mmol/L(G-1组)、15 mmol/L(G-2组)的培养液,进行体外成熟(IVM)处理,42 h后观察,并统计卵丘细胞扩散情况和第一极体排出率;对体外成熟42 h后的卵母细胞孤雌激活,统计2-细胞、4-细胞和第7天囊胚发育。结果发现,G-1组和G-2组卵丘细胞扩散度显著低于C组(P<0.05);G-1组和G-2组的MII期卵母细胞死亡率和存活率与C组相比无显著差异(P>0.05),但G-1组极体率显著降低(P<0.05),G-2组极体率极显著低于C组(P<0.01)。孤雌激活后,与C组相比,G-1组和G-2组的2-细胞分裂率显著降低(P<0.05),4-细胞分裂率以及囊胚发育率均极显著降低(P<0.01),但G-1、G-2组囊胚细胞数量与C组相比无显著性差异(P>0.05)。进一步线粒体染色发现,G-1组和G-2组的线粒体与C组相比分布不均。与C组相比,荧光结果显示G-1组和G-2组不仅活性氧(ROS)水平极显著升高(P<0.01),而且G-1组与G-2组谷胱甘肽(GSH)含量显著降低(P<0.05),虽然G-1组丙二醛(MDA)无显著性差异(P>0.05),但G-2组丙二醛(MDA)含量显著升高(P<0.05)。研究结果表明,葡萄糖浓度高会影响猪卵母细胞线粒体分布,氧化应激水平升高,成熟效率降低,损害早期胚胎的发育潜能。 相似文献
14.
试验旨在研究没食子酸（gallic acid，GA）对黄牛卵母细胞体外成熟及早期胚胎发育的影响，进一步优化黄牛卵母细胞体外成熟体系。在卵母细胞体外成熟液（M液）中添加不同浓度没食子酸（0、10、30、50、100 μmol/L），成熟22~24 h后，统计卵丘扩展情况及卵母细胞成熟率；同时，对成熟的卵母细胞进行正常体外受精（IVF），统计早期胚胎的分裂率、囊胚率、囊胚卵裂球数及卵裂球细胞凋亡率。根据试验结果，选择最优浓度，使卵母细胞在含该浓度没食子酸的成熟液中成熟24 h后，检测其细胞内的活性氧水平（ROS）和总谷胱甘肽（TGSH）含量。结果显示，M液中添加30 μmol/L没食子酸组卵丘扩展分值和成熟率显著高于对照组（0 μmol/L）（P<0.05），其他处理组与对照组无显著差异（P>0.05）；成熟的卵母细胞体外受精后进行后续胚胎培养，其中10和30 μmol/L组的分裂率均显著高于对照组和100 μmol/L组（P<0.05），50和100 μmol/L组分裂率较对照组也有所提高，但差异不显著（P>0.05）；早期囊胚率统计发现，与对照组相比，30和100 μmol/L能够显著提高囊胚发育率（P<0.05），10和50 μmol/L浓度组则无显著差异（P>0.05）；与对照组相比，30、100 μmol/L没食子酸均能显著提高IVF胚胎的早期囊胚卵裂球数（P<0.05）；但囊胚卵裂球凋亡率与对照组无显著差异（P>0.05）；对卵母细胞内活性氧和总谷胱甘肽含量检测时，发现30 μmol/L没食子酸可显著降低细胞内活性氧水平（P<0.05），且显著提高总谷胱甘肽含量（P<0.05）。综上所述，在黄牛卵母细胞体外成熟液中添加适量的没食子酸能有效降低卵母细胞内活性氧水平，提高总谷胱甘肽含量，进而提高卵母细胞成熟的质量及其后续IVF胚胎发育能力。 相似文献
15.
Effects of ovary storage time and temperature on DNA fragmentation and development of porcine oocytes 总被引：3，自引：0，他引：3
Wongsrikeao P Otoi T Karja NW Agung B Nii M Nagai T 《The Journal of reproduction and development》2005,51(1):87-97
The present study was conducted to investigate the effects of storage time and temperature of porcine ovaries on the quality and nuclear maturation in vitro of oocytes obtained from stored ovaries and their subsequent development after in vitro fertilization. The ovaries were stored in physiological saline for 0, 3, 6, 9 and 12 h at various temperatures (4, 15, 25 and 35 C). The pH of follicular fluid obtained from the ovaries, DNA fragmentation of the oocyte nucleus and meiotic competence of oocytes were examined. Some oocytes from ovaries stored at 15, 25 and 35 C for 6 h were fertilized in vitro, and then cultured for 7 days to examine the ability of embryos to develop to the blastocyst stage. When the ovaries were stored at 35 C, the pH of follicular fluid decreased and the proportions of oocytes with DNA fragmented nuclei increased as the storage time was prolonged, and the storage of ovaries for 6, 9 and 12 h resulted in lower maturation rates of oocytes. When the ovaries were stored at 4, 15, 25 and 35 C for 6 h, the storage at higher temperatures (> or =15 C) decreased the pH of follicular fluid and induced nucleic DNA fragmentation in higher proportions of oocytes. None of the oocytes from ovaries stored at 4 C reached metaphase II. The storage of ovaries at 15 C reduced the rates of in vitro fertilized oocytes and subsequent embryo development, but there were no significant differences in the rates of fertilization and blastocyst formation between oocytes from ovaries stored at 25 C and 35 C. Our findings indicate that the storage of ovaries at 25-35 C for 6 h is effective for maintaining the developmental competence of porcine oocytes even though the development rates were lower than those of ovaries stored at 35 C for 3 h. 相似文献
16.
本研究比较了不同来源的水牛卵母细胞的体外受精及其胚胎发育。对10头摩拉母水牛连续6周进行活体采卵,共采集292枚卵母细胞,头均回收卵母细胞4.87枚,头均A级卵数为3.07枚,从屠宰场收集74头水牛卵巢共采集559枚卵母细胞,头均回收卵母细胞和A级卵母细胞分别为7.55枚和5.20枚,均显著高于活体采集的水牛卵母细胞(P<0.01)。将收集的AB级水牛卵母细胞在相同的条件下进行体外成熟、体外受精和体外培养至囊胚。结果表明,活体采卵组和屠宰水牛卵母细胞组受精分裂率差异不显著,分别为54.81%和57.73%。屠宰水牛卵母细胞组的囊胚率则高于活体采卵组(28.78%vs21.34%,P<0.05)。利用两组的胚胎进行常规法冷冻保存,冻胚解冻后的存活率差异不显著(P>0.05)。 相似文献
17.
为了系统研究颗粒细胞对水牛卵母细胞体外成熟的影响,使用颗粒细胞条件液处理或单层颗粒细胞和卵母细胞共培养的方法,探讨颗粒细胞共培养对水牛卵母细胞体外成熟和早期胚胎发育的影响。结果显示,添加颗粒细胞传代接种第2天收集的20%颗粒细胞条件液到水牛卵母细胞成熟液中能显著提高水牛卵母细胞体外成熟率和囊胚发育率(P<0.05);然而单层颗粒细胞却显著抑制水牛卵母细胞体外成熟和早期胚胎发育(P<0.05)。结果表明,与单层颗粒细胞共培养相比,与颗粒细胞条件液共培养更有利于水牛卵母细胞体外成熟和早期胚胎发育。本研究为水牛卵母细胞体外成熟培养体系的完善提供一定理论依据。 相似文献
18.
P Santos A Marques G Antunes A Chaveiro M Andrade A Borba F Moreira da Silva 《Reproduction in domestic animals》2009,44(5):783-787
The overall aim of the present study was to evaluate in vitro development ability of oocytes recovered from 56 Holstein Frisian heifers with low [group 1 (G1): <13 mg /dl], moderate [group 2 (G2): 13–16 mg /dl] and high [group 3 (G3): >16 mg /dl] plasma urea nitrogen (PUN) concentrations, to determine whether PUN concentrations affect the competence of oocytes to progress to blastocysts after in vitro fertilization. In vitro oocyte and embryo development was assessed by blastocyst rates, embryo total cell numbers and apoptosis. Blood samples for the determination of PUN were collected 24 h prior to collection of the ovaries at the slaughter. A total of 112 ovaries were collected at a local abattoir and oocytes (n = 697) were aspirated, in vitro matured and fertilized. On day 8, blastocysts were assigned to the terminal dUTP nick end labelling assay. Cleavage rates were significantly higher (p < 0.001) for groups 1 and 2 than for group 3 (i.e. 72.5% and 72.2% vs 61.7%, respectively). The proportion of fertilized oocytes that developed into blastocysts was higher (p < 0.05) for group 1 than for group 3 (34.0% vs 23.0%, respectively). Day 8 blastocysts showed higher total cell counts (p < 0.05) for group 1 than for group 3 (123.7 vs 76.3), and a higher (p < 0.05) total apoptotic cell rate was found in group 3 (25.9 and 19.0 vs 43.2 for G1, G2 and G3, respectively). In conclusion, the ability of oocytes from heifers with increased levels of PUN to develop to the blastocyst stage was significantly reduced when standard routines for in vitro maturation, fertilization and culture were followed. These detrimental effects can be mediated in part through direct effect of urea and/or by the metabolic products on the process of follicle-enclosed oocyte nuclear and cytoplasmic development. 相似文献
19.
SHI Xian XIONG Xian-rong LAN Dao-liang HU Jia-jia CAI Wen-yi ZHANG Da-wei LI Jian 《中国畜牧兽医》2017,44(1):155-160
This study was designed to investigate the effect of different types and different concentrations of sugar on in vitro maturation(IVM) and developmental competence of yak oocytes, for being further research and optimization culture system of yak oocytes for efficient maturity yak oocytes and productivity of embryos. Immature yak oocytes were matured in vitro on culture medium with different concentrations (0,5 and 10 mmol/L) of glucose and sucrose in incubator for 24 h or 2 h pretreament with sugar and 22 h without sugar. Subsequently, then the maturation of oocytes,the cleavage rates and blastocyst formation rates after in vitro fertilization(IVF) were evaluated. The results showed that a medium with 5 and 10 mmol/L glucose IVM could significantly increase the yak oocytes maturation and cleavage (P<0.05), and the highest blastocyst formation rates in 10 mmol/L glucose group was significantly higher than 0 mmol/L glucose (P<0.05).10 mmol/L sucrose could increase significantly the nucleus maturation rates (P<0.05),and there was no significant difference of the blastocyst formation rates after IVF between 0 and 10 mmol/L sucrose (P>0.05). Furthermore, the nucleus maturation rates,IVF cleavage rates and blastocyst formation rates of yak oocytes which pretreated with 10 mmol/L glucose were the highest in these groups, and were higher than 0 mmol/L glucose (P<0.05). It manifested that the appropriate concentration of sugar could improve the quality of yak oocytes and embryos in vitro developmental competence, so it influenced in vitro development of yak oocytes indirectly. 相似文献