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1.
试验旨在探讨日粮不同非纤维性碳水化合物/中性洗涤纤维比(NFC/NDF)对奶山羊瘤胃发酵参数的影响.选择6只安装有永久性瘤胃瘘管的关中奶山羊作为试验动物,采用自身对照试验法,共分为4组(分4期进行),每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮.结果表明,随着日粮NFC/NDF比的提高瘤胃pH值降低,瘤胃pH值下降速率和下降幅度及pH值低于5.2、5.5、5.8、6.0的持续时间随之加快、延长;瘤胃丙酸、丁酸及总挥发性脂肪酸(TVFA)含量随日粮NFC/NDF比提高呈增加趋势,而乙酸含量及乙酸/丙酸则呈降低趋势;血浆乙酸、丙酸、丁酸及TVFA均呈增加趋势.当日粮NFC/NDF比达2.58时,瘤胃pH值在5.2~5.5之间持续时间每日达7h以上,此时瘤胃乳酸含量较低,血浆及瘤胃TVFA含量显著提高(P<0.05).结果表明,随着日粮NFC/NDF比的提高,使瘤胃逐渐处于酸性环境,改变了瘤胃发酵模式;瘤胃pH值的下降主要源于瘤胃TVFA增多,而非乳酸;当日粮NFC/NDF比为2.58时,奶山羊发生亚急性瘤胃酸中毒.  相似文献   

2.
本试验旨在探讨日粮不同非纤维性碳水化合物/中性洗涤纤维比(NFC/NDF)对奶山羊瘤胃发酵参数的影响.选择6只安装有永久性瘤胃瘘管的关中奶山羊作为试验动物,采用自身对照试验法,共分为4组(分4期进行).每期10 d,依次饲喂NFC/NDF比分别为1.02(I期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮.结果表明,随着日粮NFC/NDF比提高瘤胃pH降低,瘤胃pH下降速率和下降幅度及pH低于5.2、5.5、5.8、6.0的持续时间随之加快、延长;瘤胃丙酸、丁酸及总挥发性脂肪酸(TVFA)含量随日粮NFC/NDF比提高呈增加趋势,而乙酸含量及乙酸/丙酸则呈降低趋势;血浆乙酸、丙酸、丁酸及TVFA均呈增加趋势.当日粮NFC/NDF比达2.58时,瘤胃pH在5.2~5.5之间持续时间每日达7 h以上,此时瘤胃乳酸含量较低,血浆及瘤胃TVFA含量显著提高(P<0.05).这表明,随着日粮NFC/NDF比的提高,使瘤胃逐渐处于酸性环境,改变了瘤胃发酵模式;瘤胃pH的下降主要源于瘤胃TVFA增多,而非乳酸;当日粮NFC/NDF比为2.58时,奶山羊发生亚急性瘤胃酸中毒.  相似文献   

3.
试验旨在探讨日粮不同非纤维性碳水化合物/中性洗涤纤维比(NFC/NDF)对奶山羊瘤胃发酵参数的影响。选择6只安装有永久性瘤胃瘘管的关中奶山羊作为试验动物,采用自身对照试验法,共分为4组(分4期进行),每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮。结果表明,随着日粮NFC/NDF比的提高瘤胃pH值降低,瘤胃pH值下降速率和下降幅度及pH值低于5.2、5.5、5.8、6.0的持续时间随之加快、延长;瘤胃丙酸、丁酸及总挥发性脂肪酸(TVFA)含量随日粮NFC/NDF比提高呈增加趋势,而乙酸含量及乙酸/丙酸则呈降低趋势;血浆乙酸、丙酸、丁酸及TVFA均呈增加趋势。当日粮NFC/NDF比达2.58时,瘤胃pH值在5.2~5.5之间持续时间每日达7h以上,此时瘤胃乳酸含量较低,血浆及瘤胃TVFA含量显著提高(P〈0.05)。结果表明,随着日粮NFC/NDF比的提高,使瘤胃逐渐处于酸性环境,改变了瘤胃发酵模式;瘤胃pH值的下降主要源于瘤胃TVFA增多,而非乳酸;当日粮NFC/NDF比为2.58时,奶山羊发生亚急性瘤胃酸中毒。  相似文献   

4.
试验采用动态pH值连续监测记录系统对瘤胃pH值进行24h连续监测,旨在研究不同非纤维性碳水化合物/中性洗涤纤维比(NFC/NDF比)日粮对奶山羊瘤胃pH值昼夜动态变化的影响。选用6只安装有永久性瘤胃瘘管的关中奶山羊作为试验动物,采用自身对照试验设计,分为4期,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮。结果表明,瘤胃pH值随着日粮NFC/NDF比增加而显著降低(P<0.05),瘤胃pH值<5.2、5.5、5.8、6.0的平均持续时间亦随日粮NFC/NDF比增加而延长,分别由Ⅰ期的0、0、2.42和9.40h/d增加到Ⅳ期的0.33、7.50、17.06和20.53h/d;瘤胃pH值<5.5、5.8、6.0的曲线面积亦逐步加大,分别由Ⅰ期的0、0.21和1.43(pH·h)增加到Ⅳ期的1.09、4.84和8.59(pH·h);而且瘤胃pH值下降速率和下降幅度及最低值所持续的时间亦随日粮NFC/NDF比增加而增加。  相似文献   

5.
试验采用动态pH值连续监测记录系统对瘤胃pH值进行24 h连续监测,旨在研究不同非纤维性碳水化合物/中性洗涤纤维比(NFC/NDF比)日粮对奶山羊瘤胃pH值昼夜动态变化的影响.选用6只安装有永久性瘤胃瘘管的关中奶山羊作为试验动物,采用自身对照试验设计,分为4期,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮.结果表明,瘤胃pH值随着日粮NFC/NDF比增加而显著降低(P<0.05),瘤胃pH值<5.2、5.5、5.8、6.0的平均持续时间亦随日粮NFC/NDF比增加而延长,分别由Ⅰ期的0、0、2.42和9.40 h/d增加到Ⅳ期的0.33、7.50、17.06和20.53 h/d;瘤胃pH值<5.5、5.8、6.0的曲线面积亦逐步加大,分别由Ⅰ期的0、0.21和1.43(pH·h)增加到Ⅳ期的1.09、4.84和8.59(pH·h);而且瘤胃pH值下降速率和下降幅度及最低值所持续的时间亦随日粮NFC/NDF比增加而增加.  相似文献   

6.
不同NFC/NDF比日粮对奶山羊瘤胃pH动态变化的影响   总被引:3,自引:0,他引:3  
试验采用动态pH连续监测记录系统对瘤胃pH进行24h连续监测,旨在研究不同非纤维性碳水化合物/中性洗涤纤维比(NFC/NDF比)日粮对奶山羊瘤胃pH昼夜动态变化的影响。选用6只安装有永久性瘤胃瘘管的关中奶山羊作为试验动物,采用自身对照试验设计,分为4期,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮。结果表明:瘤胃pH随着日粮NFC/NDF比增加而显著降低(P<0.05),瘤胃pH<5.2、5.5、5.8、6.0的平均持续时间亦随日粮NFC/NDF比增加而延长,分别由Ⅰ期的0、0、2.42h/d和9.40h/d增加到Ⅳ期的0.33、7.50、17.06h/d和20.53h/d;瘤胃pH<5.5、5.8、6.0曲线面积亦逐步加大,分别由Ⅰ期的0、0.21和1.43(pH·h)增加到Ⅳ期的1.09、4.84和8.59(pH·h);而且瘤胃pH下降速率和下降幅度及最低值所持续的时间亦随日粮NFC/NDF比增加而增加。  相似文献   

7.
旨在观察不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响。选用6只安装有永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物,试验分四期进行,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种日粮,以逐渐增加日粮精料的方式诱导奶山羊发生亚急性瘤胃酸中毒(SARA)。分别利用pH动态监测记录系统和滚管培养法测定瘤胃pH、瘤胃细菌总数、淀粉分解菌及坏死梭形杆菌的浓度;以鲎试验试剂法和荧光分光光度法测定内毒素和组胺含量。结果表明:随着日粮NFC/NDF比的增大,瘤胃pH显著降低(P0.05),并且瘤胃pH下降速率和下降幅度也随之加快;日粮NFC/NDF比为2.58时,瘤胃内组胺和内毒素的浓度明显增加(P0.05),坏死梭形杆菌和淀粉分解菌的数量也显著升高(P0.01)。内毒素、组胺和坏死梭形杆菌三者浓度的异常增加对SARA发生和发展起重要作用。  相似文献   

8.
为研究鄱阳湖草型日粮不同非纤维性糖类(NFC)与中性洗涤纤维(NDF)比例对锦江黄牛瘤胃体外发酵功能及对瘤胃细菌蛋白(BCP)和总挥发性脂肪酸(TVFA)浓度影响,试验设计0.42、0.52和0.593个不同NFC/NDF值处理组,每处理5个重复,采用体外批次培养法培养24 h,测定瘤胃发酵指标。结果表明:随着NFC/NDF值的提高,产气量逐步增大但各组差异不显著(P0.05),pH各组显著下降(P0.05),氨态氮各组显著提高(P0.05);BCP浓度随着NFC/NDF值的提高而提高,其中0.51和0.59组的BCP浓度显著高于0.42组(P0.05),0.51和0.59组间差异不显著(P0.05);TVFA随着NFC/NDF值的提高而提高,其中0.59组的TVFA浓度显著高于0.42和0.51组(P0.05);丙酸浓度随着NFC/NDF值的提高而提高,各组间差异显著(P0.05);乙酸与丁酸浓度各组差异不显著(P0.05);乙酸/丙酸值随着NFC/NDF比值的提高而降低,各组间差异显著(P0.05);BCP和TVFA浓度与NFC/NDF呈正相关,其回归方程:Y=0.360 5X-0.32(Y为培养液中BCP浓度,X为NFC/NDF比,R2=0.798 2)Y=0.013X-0.4652(Y为培养液中TVFA浓度,X为NFC/NDF比,R2=0.921 8)结果表明:在研究条件下,在0.42~0.59,提高NFC/NDF值,有利于提高瘤胃发酵功能,促进BCP合成和瘤胃TVFA的产生,促进瘤胃丙酸型发酵,试验条件下以NFC/NDF比值为0.59试验组最好,但最佳NFC/NDF值需进一步研究。  相似文献   

9.
试验研究了在不同非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)比饲粮条件下诱发奶山羊亚急性瘤胃酸中毒(subacute rumen acidosis,SARA)过程中瘤胃微生物区系和瘤胃pH值的变化.该试验选用6只安装了永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物,采用自身对照试验法,共分4期进行,每期10d,依次饲喂NFC/NDF比为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种饲粮,以逐渐增加饲粮精料的方法诱导奶山羊发生SARA.结果表明,随着饲粮NFC/NDF比的增大,瘤胃pH值显著降低(P<0.05),并且瘤胃pH值下降幅度也随之加快;随着饲粮NFC/NDF比的增加,淀粉分解菌的数量增幅最显著(P<0.01);饲粮NFC/NDF比为1.63时,瘤胃细菌总数、乳酸杆菌及坏死梭形杆菌的数量显著增加(P<0.05),当该比值为2.58时,埃氏巨型球菌和反刍兽新月单胞菌的数量出现显著增多(P<0.05);原虫数量在Ⅳ期降至最低,而牛链球菌的数量在整个试验期并未出现明显的波动.饲粮NFC/NDF比为1.63时,瘤胃内与碳水化合物分解有关的多数细菌的数量明显增加,SARA期时增幅更为明显,而此时原虫数量为最低.  相似文献   

10.
试验旨在探讨在饲粮不同非纤维性碳水化合物/中性洗涤纤维条件下,奶山羊发生亚急性瘤胃酸中毒(subacute rumen acidosis,SARA)过程中瘤胃溶纤维丁酸弧菌、牛链球菌及埃氏巨型球菌数量的变化。选用6只安装有永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物,采用动物自身前后对照法,试验分4期进行,每期10d,依次饲喂NFC/NDF比分别为1.02(Ⅰ期)、1.24(Ⅱ期)、1.63(Ⅲ期)、2.58(Ⅳ期)的4种饲粮,以逐渐增加饲粮精料含量的方式诱导奶山羊发生SARA,并采用实时定量PCR技术对瘤胃内溶纤维丁酸弧菌、牛链球菌及埃氏巨型球菌的数量变化进行定量分析。结果表明:①随着饲粮NFC/NDF比的逐步升高,溶纤维丁酸弧菌和埃氏巨型球菌的数量均增加,但当饲粮NFC/NDF比升至2.58时,与其他试验期相比,埃氏巨型球菌的数量极显著增加(P<0.01),而溶纤维丁酸弧菌的数量却极显著降低(P<0.01);②牛链球菌的数量随着饲粮NFC/NDF比的逐步增加呈显著下降趋势(P<0.05),但到第Ⅳ期又恢复到第Ⅰ期的数量。结果提示,当奶山羊发生SARA时,瘤胃牛链球菌的数量无明显变化,对低pH值敏感的溶纤维丁酸弧菌数量急剧下降,而耐酸性的埃氏巨型球菌数量表现为大幅增加。  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

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An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

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正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

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