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为了解辽宁省猪圆环病毒2型（PCV2）感染情况,对来自辽宁省14个市的59个规模化商品猪场的734份血清、17个种猪场的329份血清、45户农村散养户的146份血清,采用ELISA（酶联免疫吸附试验）方法进行PCV2抗体检测。结果显示,59个规模化猪场中有51个为阳性,阳性率86.4%（51/59）,其734份样品中有351份为阳性,样品阳性率为47.8%（351/734）;17个种猪场中有15个为阳性,阳性率为88.2%（15/17）,其种猪群样品阳性率为47.6%（81/170）,仔猪群样品阳性率为29.6%（47/159）;45户散养户中有23户为阳性,阳性率为51.1%（23/45）,检测的146份样品有58份阳性,样品阳性率39.7%（58/146）。可见,PCV2在辽宁省各地的感染已经很普遍,并且种猪群感染率高于仔猪群,规模场的感染率高于农村散养户。 相似文献
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《黑龙江畜牧兽医》2018,(24)
为了了解湖南省部分猪场种猪群弓形虫感染情况及弓形虫感染对病毒疫苗(猪瘟、蓝耳病、口蹄疫)抗体和生产成绩的影响,试验采用ELISA抗体检测方法对湖南省7个市14个猪场(分别来自3个不同种源)送检的184份种猪血清进行猪弓形虫抗体的检测,同时又进行了猪瘟病毒抗体、猪蓝耳病病毒抗体和O型猪口蹄疫病毒抗体检测,同时结合生产成绩进行分析。结果表明:各地区猪场猪均有弓形虫感染,平均抗体阳性率为58. 7%,其中常德市最高,达到90. 0%。从14个猪场的种猪来源分析,种源不同,弓形虫抗体阳性率存在差异(47. 1%～69. 0%),就算是同一种源的不同猪场,弓形虫感染情况也相差比较大。弓形虫的感染情况与猪群的健康状况有关,猪群弓形虫抗体OD_(450)值平均值越高,猪瘟病毒的免疫抗体越低,弓形虫抗体OD450值平均值在2. 000以上的猪群,蓝耳病病毒抗体水平则有明显下降趋势;猪场生产成绩与弓形虫的感染情况无明显关联。说明该地区猪弓形虫感染较普遍,在猪群健康度较好的情况下,猪场生产成绩与弓形虫的感染情况无明显关联,但弓形虫的感染程度对其他病毒的免疫抗体水平有间接的影响。 相似文献
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为初步调查碧江区部分猪场弓形虫病感染情况，2021年6月—2022年6月采用间接血凝试验(IHA)检测碧江区30个猪场850份血清样品的弓形虫抗体阳性率。90份血清样品检测为弓形虫抗体阳性，平均阳性率为10.59%(90/850);5个乡(镇)来源猪血清样品弓形虫抗体阳性率分别为7.92%、4.38%、10.00%、21.67%、13.33%。不同发育阶段猪群弓形虫抗体阳性率存在差异，其中经产母猪较其他年龄阶段猪群阳性率高；经常出现野猫和老鼠的猪场来源样品弓形虫抗体阳性率高于出没频率低的猪场。研究结果表明：碧江区部分猪场弓形虫感染情况较为严重，猪场应定期进行弓形虫抗体监测，同时注意灭鼠和驱猫等工作。 相似文献
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《养猪》2019,(2)
研究旨在初步了解平顶山市猪弓形虫病感染情况,于2018年3—11月采集平顶山市部分地区14个猪场共742份猪血清样品,应用ELISA(酶联免疫吸附试验)检测试剂盒对血清样品中弓形虫抗体进行检测与分析。结果显示,742份猪血清样品中有267份检测为弓形虫抗体阳性,平均阳性率为35.98%;不同养殖模式猪场弓形虫抗体检出率存在较大差异,其中散养场最高(57.39%),种猪场稍高(38.14%),规模化猪场最低(18.56%);在被调查的4个县市中,舞钢市、郏县、叶县和鲁山的猪弓形虫抗体检出率分别为48.82%、48.44%、39.62%和18.65%。调查结果说明,河南平顶山市猪弓形虫感染普遍存在,相关有效的防制措施急需制定与实施。 相似文献
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通过对国内猪、牛和鸡弓形虫感染情况及危险因素的综述分析发现,各地的猪弓形虫血清阳性率差异较大,其中重庆市猪弓形虫感染阳性率有的高达71.9%;分析认为猪弓形虫感染的危险因素主要是性别、品种、饲养方式和养殖环境等。甘肃省、河南省的牛弓形虫感染率分别为4.8%和5.4%,新疆牛弓形虫血清阳性率最高的达到46.4%;与犬猫接触、年龄及饲养方式等是影响牛弓形虫感染的风险因素。甘肃省、辽宁省的鸡弓形虫感染率分别为7.3%和9.0%,广东的鸡弓形虫感染率最高的达20.3%;鸡弓形虫感染与饲养方式密切相关。 相似文献
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为全面了解贵州省猪弓形虫感染情况,对采自9个市（州、地）264个养猪场（户）的2 906份血清用酶联免疫吸附试验（ELISA）检测猪弓形虫抗体,总体阳性率为65.83%,变化范围为27.88%~85.42%。采集65份猪血清做ELISA和间接血凝试验（indirect heamagglutination assay,IHA）比较测定,两种方法总体符合率为58.46%。IHA方法补充检测177份猪血清,弓形虫抗体阳性率为27.68%。血清学调查结果与国内部分省（市）报道相符。 相似文献
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《养殖与饲料．饲料世界》2017,(9)
为了解临沧市牛、羊和犬弓形虫感染情况,分别采集临沧市的临翔区、双江县、凤庆县和沧源县农户、养殖场和餐馆的动物血样品284份(牛143份、羊81份、犬60份),分离血清,采用间接血凝试验(IHA)进行弓形虫抗体检测。结果表明,弓形虫抗体阳性53份,阳性率18.6%,其中牛血清弓形虫抗体阳性20份,阳性率为14%;羊血清弓形虫抗体阳性19份,阳性率为23.5%;犬血清弓形虫抗体阳性14份,阳性率为23.3%。综上所述,临沧市牛羊犬弓形虫抗体阳性率较高,有兽医公共卫生安全隐患。 相似文献
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为了解洛阳地区规模化养猪场和散养户猪弓形虫感染的情况,采集洛阳地区规模化养殖场和散养户母猪、育肥猪、保育猪血清样品410份,应用ELISA方法检测抗弓形虫IgG抗体。采集河南科技大学动物医院接诊的病死猪54头及洛阳某食品公司屠宰猪105头的肺门淋巴结,用PCR方法扩增弓形虫特异性遗传标记529 bp基因序列进行检测。结果发现,洛阳地区猪弓形虫血清抗体阳性率达35.4%,母猪、育肥猪和保育猪的分别是50.4%、34.5%、22.2%。病死猪PCR检测阳性率18.5%,屠宰猪阳性率6.7%。说明洛阳地区猪弓形虫的感染较为普遍。 相似文献
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本文应用抗弓形虫单克隆抗体ELISA对猪弓形虫病感染进行现场调查研究。用一般ELISA的间接法、双单抗夹心法、单抗及第二抗体夹心法分别检测Ab、Cag及CIC。杭州地区一般屠宰猪血清阳性检出率分别为52.79%、0%及8.22%;有弓形虫病流行史的某种猪场母猪血清的阳性检出率分别达84.51%、32.65%及68.39%,三者均明显高于屠宰猪。14头疑似弓形虫病猪血清的阳性检出率分别为6/14、10/14及12/14,Cag或和CIC阳性率合计为14/14。初步认为应用ELISA间接法检测Ab可作为猪弓形虫感染血清流行病学检查;应用McAb ELISA夹心法检测CAg及CIC具有早期诊断猪弓形虫病的价值,并可作为历史流行点猪弓形虫病是否存在近期流行或尚有活动性感染及暴发性流行的监测方法。 相似文献
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青海省动物弓形虫病的血清学检测 总被引：1，自引：0，他引：1
应用弓形虫的间接血凝试验（IHA）,对来自青海省境内的部分土种绵羊、马属动物、藏獒、骆驼、喜马拉雅旱獭、高原鼠兔、中华鼢鼠、藏羚羊和实验小鼠等动物的1039份血清样品,进行弓形虫特异性抗体的检测。结果检出68份阳性血清,阳性率为6.54%。 相似文献
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Seroepidemiology of Neospora caninum and Toxoplasma gondii infection in yaks (Bos grunniens) in Qinghai, China 总被引：1，自引：0，他引：1
Neospora caninum is a protozoan parasite and is closely related to Toxoplasma gondii, but they are antigenically different. N. caninum and T. gondii infection in a variety of animals such as cattle, dogs, and cats has been reported, but there is little information on the infection of these parasites in domestic yaks. Seroprevalence of antibodies to T. gondii and N. caninum in yaks (Bos grunniens) from eight regions of Qinghai, China were investigated by the indirect agglutination test (IAT) and ELISA, respectively. A total of 112 (11.8%) of 946 serum samples were positive for antibodies to T. gondii, and 21 samples (2.2%) were positive to N. caninum. Two of the yaks had antibodies to both parasites. There was no apparent association of T. gondii infection with age of the animals. The results indicate that T. gondii infection is prevalent in Chinese yaks in most parts of Qinghai province and N. caninum infection rate in the same species is relatively low. This is the first large study showing the infection of T. gondii and N. caninum in domestic yaks. 相似文献
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Garcia JL Gennari SM Navarro IT Machado RZ Headley SA Vidotto O da Silva Guimarães Junior J Bugni FM Igarashi M 《Research in veterinary science》2008,84(2):237-242
The study evaluated the efficiency of diagnostic laboratory methods to detect anti-Toxoplasma gondii antibodies in paired serum and aqueous humour samples from experimentally infected pigs. 18-mixed breed pigs were used during the experiment; these were divided into two groups, G1 (infected group, n=10) and G2 (uninfected group, n=8). Infection was performed with 4 x 10(4) VEG strain oocysts at day 0 by the oral route in G1 animals. All pigs were euthanized at day 60, when retina, aqueous humour, and blood samples were collected. Anti-T. gondii antibody levels were assessed in serum (s) and aqueous humour (ah) by indirect immunofluorescence assay (IFA), modified agglutination test (MAT), m-ELISA (using crude membranes from T. gondii tachyzoites as antigen) and r-ELISA (using rhoptries from T. gondii tachyzoites as antigen). Polymerase chain reactions (PCR) of samples from the retina were performed by using Tox4 and Tox5 primers. Antibody titers of G1 animals ranged from 128 to 1024 and from 16 to 256 in serum and aqueous humour, respectively. There were differences in the correlation coefficients between IFA(s) x IFA (ah) (r=0.62, P=0.05), MAT(s) x MAT (ah) (r=0.97, P<0.0001); however, there was no significant difference between r-ELISA(s) x r-ELISA (ah) (r= 0.14, P=0.7). Antibodies present in serum and aqueous humour recognized similar antigens. Samples of retina were positive by PCR in 30% (3/10) of infected pigs. G2 animals remained without antibody levels and were PCR negative throughout the experiment. These results suggest that the use of a combination of tests and immunoblotting for paired aqueous humour and serum samples could improve the sensitivity and specificity for the diagnosis of ocular toxoplasmosis. 相似文献
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Sera from 68 nondomestic captive and free-ranging felids from southern Africa were tested for antibodies to Neospora caninum and Toxoplasma gondii by the indirect fluorescent antibody test. Four of the 68 (5.9%) serum samples were positive for antibodies to N. caninum, with titers ranging from 1:50 to 1:200. All other animals were negative for antibodies to N. caninum at a dilution of 1:50. Fifty of the 68 (74%) serum samples tested positive for antibodies to T. gondii, with titers ranging from 1:50 to 1:26,500. Four animals tested positive for antibodies to both N. caninum and T. gondii. None of these animals displayed clinical signs of disease. Results of this study indicate that nondomestic felids in southern Africa have been exposed to, and are likely infected with, N. caninum and T. gondii. 相似文献
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采用布氏杆菌虎红平板凝集试验、弓形虫和衣原体间接血凝试验对360份绒山羊血清进行布氏杆菌病、弓形虫病和衣原体病的血清学调查。结果在绒山羊的血清样品中检出布氏杆菌病阳性血清8份,布氏杆菌病的血清阳性率为2．22％;检出弓形虫15份,弓形虫病血清阳性率为4．17％;检出衣原体23份,衣原体病血清阳性率为6．39％。 相似文献