共查询到19条相似文献，搜索用时 218 毫秒
1.
《中国兽医杂志》2015,(7)
为了研究鸡免疫器官中细胞因子的基础转录水平,本试验采用SYBR Green I实时荧光定量PCR方法,检测56日龄鸡免疫器官中Th1类细胞因子及Th2类细胞因子的m RNA相对表达量并进行比较。结果显示,4种细胞因子在盲肠扁桃体中的表达水平均高于腔上囊。脾脏中细胞因子表达水平总体上较同为外周免疫器官的盲肠扁桃体低。胸腺、脾脏与腔上囊中IFN-γ与IL-4的表达呈负相关性。研究表明,盲肠扁桃体作为可直接参与免疫反应的外周免疫器官,其免疫活性可能较中枢免疫器官腔上囊更高。IFN-γ与IL-4作为最具代表性的Th1与Th2类细胞因子,其表达表现为相互抑制。 相似文献
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《中国兽医杂志》2020,(1)
为研究副鸡禽杆菌(Apg)外膜囊泡(OMVs)的生物活性,提取了A型副鸡禽杆菌的外膜囊泡。纯化后的OMVs用透射电镜观察其形态结构;将OMVs加入到鸡巨噬细胞(HD-11)培养液中,检测一氧化氮(NO)产生量的变化;用荧光定量PCR(qPCR)检测HD-11各炎症相关因子的表达量变化情况;将OMVs经肌肉注射接种SPF鸡,检测鸡血清IgG抗体产生情况,并用3种血清型的副鸡禽杆菌强毒菌株攻击免疫鸡,测定其免疫保护率。结果显示,电镜下的副鸡禽杆菌OMVs呈球形,直径在20～300 nm;OMVs可极显著提高HD-11中NO产量;qPCR结果显示,OMVs作用可显著上调鸡巨噬细胞IL-2、IL-6、 IL-1β、IL-10、iNOs等炎症相关因子的表达。免疫鸡可以产生特异性的IgG抗体。用A型副鸡禽杆菌攻毒,免疫保护率为70%,对异种血清型交叉保护性较弱,仅有10%～30%。本研究为深入研究副鸡禽杆菌外膜囊泡的功能奠定了基础。 相似文献
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鸡IL-1β、IL-2、IFN-γ和MGF对表达新城疫病毒HN基因的重组鸡痘病毒免疫效力的影响 总被引：1，自引：0，他引：1
将10日龄的SPF鸡和15、28日龄的商品鸡分别随机分组,同时免疫不同剂量的rFPV-HN（表达NDV HN基因重组鸡痘病毒）和表达不同细胞因子（IL-1β、IL-2、IFN-γ、MGF）的重组鸡痘病毒（rFPVs）。在免疫后不同时间,通过检测血液中抗NDV的HN蛋白抗体、脾脏中CD4＋和CD8＋T细胞的变化及攻毒保护率等指标。结果表明：IL-1β或IFN-γ在免疫后期能促进抗HN间接ELISA抗体的滴度上升,而且产生抗体的整齐度好;IL-1β或IL-2可以显著增加脾脏中CD4＋和CD8＋T细胞含量;IL-1β、IL-2或IFN-γ同样可以提高商品鸡攻毒后的存活率。IL-2和IFN-γ在增加脾脏中CD4＋和CD8＋T细胞的数量及攻毒保护方面表现出一定的协同增强作用。 相似文献
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禽呼肠病毒感染对SPF鸡外周血T细胞亚型变化和细胞因子转录的影响 总被引：1，自引：0，他引：1
为探讨禽呼肠病毒(ARV)对SPF鸡外周血淋巴细胞中CD4+、CD8+T细胞数量变化和细胞因子mRNA转录水平的影响,利用流式细胞术和实时荧光定量PCR方法分别测定了ARV感染后1、7、14、21、28、35d感染组和对照组SPF鸡外周血淋巴细胞中CD4+、CD8+T细胞含量和细胞因子IL-1β、IL-6、IL-17、IL-18、IFN-γ、TNF-α基因mRNA相对转录时相。流式细胞术检测结果表明,SPF鸡感染ARV后7d和14dCD4+、CD8+T细胞比值高于对照组,其中感染7d,CD8+T细胞含量差异显著(P0.05);感染后1、21、28、35d感染组CD4+、CD8+T细胞比值均低于对照组,感染1d后CD4+、CD8+T细胞含量均差异显著(P0.05),说明外周血T细胞亚型变化是ARV感染的重要表现之一。实时荧光定量PCR结果表明,与对照组相比,感染组外周血淋巴细胞中IL-1β、IL-6(除7d外)、IL-18(除14d外)和TNF-α在整个感染过程中表达上调,IL-17和IFN-γ除感染1d外,均表达下调,说明IL-1β、IL-6、IL-17、IL-18、IFN-γ和TNF-α均参与了ARV的感染进程。 相似文献
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研究刺五加多糖(ASPS)对鸡淋巴细胞体外增殖及细胞因子mRNA表达水平的影响。将不同浓度的ASPS添加到体外分离培养的血液淋巴细胞中，采用噻唑蓝(MTT)法检测淋巴细胞增殖变化，以实时荧光定量PCR方法检测淋巴细胞细胞因子IFN-γ、IL-2、IL-4、IL-10、IL-1β、IL-6、iNOS和TNF-αmRNA表达水平。结果显示，ASPS可以单独或协同植物血凝素(PHA)或脂多糖(LPS)促进淋巴细胞体外增殖，并且ASPS在质量浓度为200μg/mL时效果最佳。另外，ASPS能够促进淋巴细胞细胞因子mRNA的表达，当200μg/mL的ASPS与淋巴细胞体外共培养至24 h时，IFN-γ、IL-2、IL-1β、IL-6、iNOS和TNF-α的mRNA表达量均最高，而IL-4和IL-10 mRNA的相对表达量则以ASPS质量浓度为400μg/mL和培养时间48 h条件下最高，说明IL-4和IL-10 mRNA的表达滞后，而且呈剂量依赖性。结果表明，ASPS能够提高鸡淋巴细胞的体外增殖功能，促进Th1和Th2细胞因子分泌，这为进一步揭示刺五加多糖的免疫调节机制奠定了基础。 相似文献
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为探讨鸡传染性支气管炎病毒(IBV)感染与宿主固有免疫系统的相互作用及其致病与免疫机制,本研究采用实时荧光定量RT-PCR方法检测IBV变异株GX-YL5感染鸡后不同时间点气管黏膜和哈德氏腺中固有免疫相关受体和细胞因子的mRNA水平以及IBV载量的动态变化。结果显示,气管黏膜和哈德氏腺中Toll样受体TLR2、TLR3、TLR6、TLR7、趋化因子MIP-1β和趋化因子受体CXCR4、CCR4在IBV感染后1~8天以及细胞因子IFN-α、IFN-β、IL-1β、IL-6、IL-10在IBV感染后1~5天,mRNA转录水平至少有一个时间点显著(P0.05)或极显著(P0.01)上调;感染后14~28天,一些受体和细胞因子的mRNA则出现下调;气管黏膜和哈德氏腺的IBV病毒载量在感染后立即上升,第3天达到峰值,随后下降。结果表明,IBV感染早期气管黏膜和哈德氏腺中固有免疫相关受体和细胞因子的mRNA转录水平显著上调,说明IBV感染对宿主固有免疫应答产生明显的影响。 相似文献
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Toll样受体(TLRs)是一组保守的蛋白质,除了在先天免疫和适应性免疫反应的启动和调控中发挥重要作用,还参与病原体识别。目前,已鉴定到若干个识别不同配体的鸡TLRs,在刺激鸡对病原体产生宿主反应中发挥重要作用。然而,通过哪个TLRs对鸡免疫系统进行调节尚未明了。本试验通过分别向试验鸡肌肉注射TLR4和TLR21相应的配体脂多糖(LPS)和CpG寡聚脱氧核苷酸(ODNs),对其刺激鸡免疫反应的动力学特性进行了研究。在注射TLR配体2、6、12和24h时,可在脾脏中检测到细胞因子的相应表达。结果表明,LPS能在试验早期强烈诱导免疫系统基因表达的上调,包括IFN-γ、IL-10和IL-1β。CpG ODNs能促进MHC-Ⅱ、IFN-γ和IL-10的上调。而对CpG ODNs的应答比对LPS的应答略迟。在对LPS的反应中,IFN-α基因的表达显著增加,而在对CpG ODNs的反应中无明显变化。进一步试验将对鸡TLR激活的分子机制或应用TLR激动剂作为疫苗佐剂进行研究。 相似文献
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分别采用新城疫强毒株F48E9与新城疫弱毒株La Sota感染10日龄鸡胚制作的鸡胚成纤维细胞,感染后24 h提取鸡胚成纤维细胞的总RNA,通过RT-PCR技术结合凝胶成像系统扫描检测21种免疫相关细胞因子在鸡胚成纤维细胞中的表达量.实验结果表明,在鸡胚成纤维细胞中,无论是否感染新城疫病毒,IL-2、IL-3、IL-4、IL-21不能检测到其相应条带.通过统计学分析,IFN-β、IFN-γ、IL-9、IL-12-P35、IL-12-P40在病毒感染组与对照组间呈现出显著的差异,而强毒感染组与弱毒感染组的比较中,仅IFNγ、IL-9表现出显著差异. 相似文献
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试验旨在从免疫遗传学的角度初步探讨藏鸡（TC）和隐性白羽鸡（RWC）对柔嫩艾美耳球虫（Eimeria tenella）易感性差异的分子机制,分别用1×105个柔嫩艾美耳球虫孢子化卵囊感染对球虫具有抗性的藏鸡和易感的隐性白羽鸡。应用实时荧光定量PCR检测藏鸡和隐性白羽鸡感染前0 d和感染后第2、4、6和8天脾脏、盲肠、胸腺、法氏囊中γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）、IL-16、Toll样受体（TLR3）和TLR15免疫相关基因的转录水平变化。结果显示,藏鸡脾脏IFN-γ、IL-2、IL-16及TLR3、TLR15免疫相关基因转录水平于感染后第4和8天明显上调,隐性白羽鸡则无明显变化。藏鸡盲肠IFN-γ转录水平在感染后第2天显著上调（P<0.05）,TLR3在感染后第4天起显著上调（P<0.05）,其余免疫相关基因变化幅度不大;隐性白羽鸡盲肠IFN-γ转录水平在感染后第8天显著上调（P<0.05）,IL-2在感染后第2天起显著上调（P<0.05）,IL-16在感染后第6天起显著上调（P<0.05）,TLR3在感染后第2和8天显著上调（P<0.05）,TLR15变化幅度不大。各免疫相关基因在2个品种鸡胸腺和法氏囊中均出现上调或下调,但除藏鸡法氏囊TLR3和TLR15转录水平变化幅度相对较大外,其余免疫相关基因与感染前相比变化幅度不大。以上结果显示,球虫感染主要导致藏鸡和隐性白羽鸡脾脏和盲肠中的各免疫相关基因出现显著变化,表明宿主的遗传背景在一定程度上可影响球虫感染的免疫应答。 相似文献
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为了分析禽呼肠孤病毒(avian reovirus,ARV)S1133株感染鸡胚成纤维细胞(chicken embryo fibroblast,CEF)后,对其细胞因子IL-17、IL-18和IFN-γmRNA转录水平的影响,探讨禽呼肠孤病毒感染机制和宿主之间的作用关系。本试验将ARV-S1133感染CEF细胞后,运用荧光定量PCR技术,测定和分析ARV结构蛋白σC和CEF细胞的IL-17、IL-18及IFN-γ的mRNA动态转录水平。结果表明,ARV-S1133感染CEF细胞10h后病毒结构蛋白σC mRNA的相对表达量开始迅速上升,在48h达到最高峰(13 162.73倍);ARV-S1133感染后引起CEF细胞中的IL-17、IL-18和IFN-γmRNA表达量发生变化,IL-17和IFN-γmRNA转录水平在感染早期迅速上调,感染后6h达到第一个峰值,分别上调8.77倍和11.17倍,随后下降,在感染36、48、60h后大幅度上升,且在48h达到峰值,表达量分别为97.19倍和111.58倍。IL-18mRNA转录水平在整个感染过程中表达量较低,在感染6h后微量上调(1.39倍),在感染中后期,其表达量呈下调趋势,感染48h时,IL-18mRNA表达量最低,与对照组相比下调0.19倍;5个不同滴度的ARV病毒感染CEF细胞24h后,3个细胞因子mRNA的表达量与病毒滴度线性相关,IL-17和IFN-γmRNA转录水平与病毒滴度正相关,IL-18mRNA转录水平与病毒滴度负相关。结果表明,ARV感染后可诱导CEF分泌IL-17、IL-18、IFN-γ的mRNA转录水平上调,说明IL-17、IL-18、IFN-γ可能与禽呼肠孤病毒的复制和致病机制相关。 相似文献
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Jole Mariella Carolina CastagnettiAngelo Peli DVM PhD Maria MoriniCaterina Sorteni DVM Giuliano BettiniMarco Pietra DVM 《Journal of Equine Veterinary Science》2013
Gastritis and gastric ulcerations occur frequently in neonatal foals. The relationship between cytokines expressed by gastric mucosa and gastric histopathology in healthy or sick foals has never been investigated. The aim of this study was to compare the histological diagnosis and endoscopic view with cytokine expression (TNF-α, IL-1β, IL-4, IL-8, IL-13, and IFN-γ) of gastric mucosa. Twenty-two foals were definitively enrolled in the study: 19 were critically ill, and 3 were healthy foals. Gastric biopsy specimens were collected for histological examination and for cytokine mRNA qualitative real-time PCR analysis. This study shows that there is a substantial agreement between histology and endoscopy and that foals with evidence of gastritis and gastric ulcerations have higher probability of expressing TNF-α. Moreover, the overall profile of cytokines expression, with a low percentage of IFN-γ, a high percentage of IL-4, and the absence of IL-13, suggests a down-regulation of the Th1 cell-mediated immune response and an impaired Th2 response in the gastric wall in the neonatal period. 相似文献
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Avian β-defensins (AvβDs) are antimicrobial peptides that play significant roles in the innate immune system in chickens. The aim of this study was to identify the types of AvβDs expressed in the chicken ovary, to investigate the effects of sexual maturation in the ovarian mRNA abundance and to determine the changes in their expression levels as a result to Salmonella enteritidis (SE) infection. RNA was extracted from the ovary of healthy prepubertal, sexually mature and aged birds, as well as from sexually mature and aged SE infected birds. Real-time PCR analysis revealed that 11 AvβDs genes were expressed in the chicken ovary. A significant up regulation of AvβD1, 3, 4, 5, 7 and 11 was observed in the ovary of sexually mature and aged birds. Furthermore, a significant up-regulation of AvβD4, 5, 7, 11 and 12 was observed in the ovary of SE infected sexually mature birds. These results suggest that the mRNA expression of at least six AvβDs increase with age in the ovary of laying hens, and that at least five AvβDs show an induction in their expression in response to SE infection, indicating an AvβD-mediated immune response mechanism in the chicken ovary. 相似文献
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《Research in veterinary science》2014,96(3):957-964
Infectious bursal disease (IBD) is an acute and highly contagious viral disease of young chickens caused by infectious bursal disease virus (IBDV). An effective way to control IBDV would be to breed chickens with a reduced susceptibility to IBDV infection. In the present work, we used chickens selected for high and low specific responses to sheep red blood cells (SRBC) (H and L, respectively) to assess the susceptibility of differential immune competent animals to IBDV infection. The peripheral blood mononuclear cells (PBMCs) of high SRBC line (HL) and low SRBC line (LL) were infected with IBDV and viral RNA loads were determined at different time post-IBDV infection. Chicken orthologues of the T helper 1 (Th1) cytokines, interferon-γ (IFN-γ) and interleukin-2 (IL-2); a Th2 cytokine, IL-10; a pro inflammatory cytokine, IL-6; the CCL chemokines, chCCLi2, chCCLi4 and chCCLi7; colony stimulating factor, GM-CSF; and a anti-inflammatory cytokine, transforming growth factor β-2 (TGFβ-2) were quantified. The expression of chCCLi2, chCCLi4 and chCCLi7 was significantly higher in L line as compared to H line. However, in H line the viral RNA loads were significantly lower than in L line. Therefore, the upregulated chemokines might be associated with the susceptibility to IBDV. The expression of IFN-γ, IL-2 and IL-6 was significantly higher in H line as compared to L line. We assume that the higher proinflammatory cytokines expression in H line might be related to the rapid clearance of virus from PBMCs. Significantly higher levels of IL-10 and TGFβ-2 mRNAs in L line might be related to the pathogenesis of IBDV. In conclusion, selection for antibody responses appears to influence the expression profiles of chemokines and cytokines against IBDV. Further, the selection for high SRBC response might improve the immuno-competence of chickens against IBDV. 相似文献
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Tomoya Kono Hiroaki Takayama Ryusuke Nagamine Hiroki Korenaga Masahiro Sakai 《Veterinary immunology and immunopathology》2013,151(1-2):90-101
To monitor the expression of cytokine genes in Japanese pufferfish, a novel platform for quantitative multiplexed analysis was developed. This custom-designed multiplex RT-PCR assay was used to analyze the expression profiles of 19 cytokine genes, including pro-inflammatory (IL-1β, IL-6, IL-17A/F3, IL-18, TNF-α, TNF-N), anti-inflammatory (IL-4/13A, IL-4/13B, IL-10), T-cell proliferation/differentiation (IL-2, IL-15, IL-21, TGF-β1), B-cell activation/differentiation (IL-7, IL-6, IL-4/13A, IL-4/13B), NK cell stimulation (IL-12p35 and IL-12p40), induction of anti-viral activity (I-IFN-1 and IFN-γ), and monocyte/macrophage progenitor cell proliferation (M-CSF1b) cytokines in head kidney cells under immune stimulatory conditions. The expression profiles were dissimilar in the unstimulated control and immune-stimulated cells. Moreover, increased expression profile was observed due to different stimulations for IL-1β, IL-6, IL-10, IL-12p35, IL-12p40, IL-21, TNF-α, TNF-N, I-IFN-1 and IFN-γ genes. These results suggest that cytokine genes could be used as biomarkers to know the immune status of fish. The constructed multiplex RT-PCR assay will enhance understanding on immune regulation by cytokines in fish. 相似文献
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Maria Anastasiadou Melpomeni Avdi Alexandros Theodoridis Georgios Michailidis 《Veterinary research communications》2013,37(2):115-122
Avian β-defensins (AvβDs) constitute a family of antimicrobial peptides that are critical to innate immunity in chickens, providing protection against microbial pathogens including Salmonella Enteritidis (SE). As apart from the digestive tract another main route of SE colonization in birds is via infection of the oviduct and specifically of the vagina, the aim of this study was to investigate the expression of the complete family of AvβDs, in the chicken vagina in vivo, to determine whether sexual maturation affects their mRNA abundance and to investigate whether SE infection alters the vaginal AvβDs expression. Expression analysis revealed that 11 members of the AvβD family were expressed in the chicken vagina. Quantitative real-time PCR analysis revealed that the mRNA abundance of five AvβDs was up regulated and of one AvβD was down regulated with respect to sexual maturation. In addition SE infection resulted in a significant induction of AvβD5, 7, 10, 11, 12 and 14 in the vagina of sexually mature birds, and in a significant induction of AvβD5 and 11 in the vagina of aged birds. These findings provide strong evidence to suggest that an AvβD-mediated immune response mechanism exists in the chicken vagina providing protection against bacterial pathogens including Salmonella species. 相似文献
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本试验旨在探明绵羊感染16型蓝舌病病毒(Bluetongue virus type 16,BTV16)后细胞因子IFN-γ、IL-2、IL-4和IL-10的消长特点。用实时荧光定量PCR检测方法对感染BTV16后3只绵羊上述4种因子mRNA进行检测,同时设立阴性对照绵羊,并以0 d mRNA为基准,计算mRNA的相对表达量,同时检测病毒抗体效价、测量绵羊体温。结果显示,接种BTV16的3只绵羊均不同程度产生抗体和体温症状,4种细胞因子的mRNA在接种病毒2~4 d内均出现显著上升,其中IFN-γ峰值在2.58~27.84倍之间,IL-2峰值在5.24~17.19倍之间,IL-4峰值在2.16~3.43倍之间,IL-10峰值在15.78~48.77倍之间,个体上升幅度存在显著差异,4种细胞因子均在高水平持续6 d左右后逐渐下降。对照绵羊上述参数在正常范围内波动。本研究阐明了接种BTV16后绵羊细胞因子IFN-γ、IL-2、IL-4、IL-10在转录水平上的消长特点,为进一步深入开展BTV感染特征、宿主机体免疫机制研究提供参考。 相似文献