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采用锅炉加热，塑料大棚增温、保温，分三级强化培育早繁大眼幼体８ｋｇ，当年育出６０—１２０尾／ｋｇ的大规格幼蟹３５万只。出售Ⅴ—Ⅵ期幼蟹利润１２９万元。 相似文献
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河蟹人工育苗高产技术研究 总被引：1，自引：0，他引：1
研究了蓄水池及其处理、亲蟹暂养、布苗方法、幼体培育一整套河蟹育苗高产技术，在５７６ｍ＾３水体中大眼纪估３１２．５ｋｇ，单位水体出苗量５４０ｇ／ｍ＾３；蚤状本Ｚ１变大眼幼体平均或活率达到２８．６％，发现一种可疑病原菌。大眼纪体最适培育 １０万尾／ｍ＾３。 相似文献
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河蟹人工育苗幼体饵料的研究 总被引：3，自引：0，他引：3
河蟹蚤状幼体前期以单胞藻、轮虫为主，后期以鱼糜为主，大眼幼体后以枝角类为主的饵料系列，作为河蟹人工育苗幼体饵料，成功育出了大眼幼体。１９９２年育苗６１３万只，平均出苗量９．７３万只／米＾３，平均成活率４４．８％。以鱼糜代替丰年虫的饵料成本仅为７％。 相似文献
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罗氏沼虾工厂化育苗试验 总被引：2，自引：0，他引：2
利用对虾育苗场的设计进行罗氏沼虾工厂化育苗试验，于１３６ｍ＾３水体中投放状幼体１４３８万尾，育出商品仔虾９６４万尾，幼体培育成活率为６７％，平均每尾亲虾出苗４７２５尾，单位水体平均出苗量为７．０９万尾，最高为１０．２万尾／ｍ＾３。从４月上旬到５月下旬，罗氏沼虾雌性亲虾可抑卵三次以上，前后抱卵批次所孵出的幼体质量没有明显差异。试验还证明，幼体培育池内氨氮浓度高达１０５６．４μｇ／ｌ对成活率没有多大曩 相似文献
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１９９６￣１９９７年，在北方地区首次利用４２０ｍ＾２塑料大棚土池，放养大眼幼体４９．５万只，进行培育大规格幼蟹试验。采取清池肥水，布放水草，科学投饵，控制水质等措施，共出池Ⅴ期以上幼蟹１６．４万只，平均成活率３３．１３％，专家鉴定认为，本研究达国内先进水平。 相似文献
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５．３ｈｍ＾２稻田，投放１５００ｇ河蟹大眼幼体在暂养池中培育４５ｄ后放入稻田，同时放养异育银卿育花３万尾，抱卵青虾１５ｋｇ经过１８０ｄ的饲养，收获稻谷１７５００ｋｇ，成蟹２１２５ｋｇ，成虾７５ｋｇ，鱼种６００ｋｇ，创产值１４８５３５元，利润７８３３５元，投入产出比１：２．１２。 相似文献
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首次在北部沿海进行了长江蟹人工繁殖试验,报告了亲蟹促产,越冬培育,饵料投喂,水质管理,幼体孵化,幼体培育等全套工艺流程,交配抱卵率达８１％,越冬成活率８４．６％,大眼幼体育成率２２．５％,认为长江蟹,黄河蟹幼体培育方法一致,培养结果,育成率无显著差异,但越冬水深应保持１．５ｍ以上,亲蟹须先进行１５￣２０天强化培育。 相似文献
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2003年4～5月，在南通东朋水产品有限公司吉成河蟹育苗场进行河蟹育苗，共育出四批大眼幼体。前两批以丰年虫无节幼体为主要饵料，1．500m^3育苗水体共育出大眼幼体370kg，平均出苗量0．25kg／m^3，蟹苗质量较好(规格为15万只／kg左右)，但育苗成本很高，为800元/kg；第三批苗以新鲜淡水蚤(桡足类)为主要饵料，丰年虫无节幼体为辅助饵料，495m^3育苗水体共育出大眼幼体99kg，平均出苗量0．20kg／m^3，蟹苗个体小(规格在44万只／kg左右)，质量差；第四批育苗完全不用丰年虫无节幼体，而是采用生态法育苗，施肥培育单细胞藻类，450m^3水体共育出大眼幼体260kg(3900万)，平均出苗量0．5kg／m^3左右，育苗成本低，大眼幼体质量好。规格在8000只／kg的幼蟹,回捕率在30％以上。 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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Growth hormone (GH) and reproduction: a review 总被引：5，自引：0，他引：5
Florence Le Gac Odile Blaise Alex Fostier Pierre-Yves Le Bail Maurice Loir Brigitte Mourot Claudine Weil 《Fish physiology and biochemistry》1993,11(1-6):219-232
Interaction between growth and reproduction occurs in many vertebrates and is particularly obvious at certain stages of the life cycle in fish. Endocrine interactions between the gonadotropic axis and the somatotropic axis are described, the potential role of GH being emphasised. A comparative analysis of these phenomena in mammals, amphibians and fish, suggests a specific role of GH in the physiology of puberty, gametogenesis and fertility. It also shows the original contribution made by studies on the fish model in this field of investigations. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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