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1.
西藏黄牛染色体的研究   总被引:5,自引:0,他引:5  
研究了西藏黄牛的普通核型和C带核型,着重分析了Y染色体的形态。结果表明,西藏黄牛核型为2N=60,公牛为XY,母牛为XX,常染色体为近端着丝粒染色体。X染色体为亚中着丝粒染色体,Y染色体中中部着丝粒杂色体。根据Y染色体的形态讨论了西藏黄牛的起源。  相似文献   

2.
丹麦红牛的染色体分析   总被引:1,自引:0,他引:1  
分析了7头(3公4母)丹麦红牛的染色体核型、C带和Ag—NoRs。结果表明,丹麦红牛二倍体为2n=60,公平核型为60,xy,母牛为60,xx,x染色体为一大的亚中着丝粒染色体,y染色体为一小的亚中着丝粒染色体,其臂比为1.722。丹麦红牛的核型和C带带型与普通牛(Bostaurus)相同。观察统计了343个细胞的Ag—NoRs数,平均每细胞为5.314±0.181,变化范围为2~9个。分析了正常半体细胞多倍体的出现率,平均为3.4±1.2%。  相似文献   

3.
采用常规外周血培养及体核型分析技术,对青海省大通牛场猎获的野牦牛染色体核型进行了研究。结果显示,野牦牛染色体数目为2n=60,染色体臂数NF=62;常染色体形态为端着丝粒,性染色体为亚中着丝粒,公野牦牛核型为60,XY。  相似文献   

4.
采用外周血淋巴细胞培养法,以 G-,C-分带和银染技术,对我国4个地方黄牛品种延安地区蒙古牛(10 ♂;5♀)、关中秦川牛(12♂;4),商洛山阳的岭南牛(6♂;5♀)以及陕南西乡的西镇牛(9♂;12)共计63头黄牛的染色体核型,G 带和C 带以及 Ag—NoRs 数目和分布进行了详细的研究。结果发现,性染色体(x 和 y)和  相似文献   

5.
陕西延安蒙古牛群体的染色体研究   总被引:6,自引:0,他引:6  
分析了15头(10♂5♀)蒙古牛的核型,C带、G带和Ag-NORs.结果发现,蒙古牛的核型,C带和G带与普通牛(Bostaurus)相同,其公平核型为60,xy母牛为60,xx.y染色体为一小的中着丝粒染色体,认为蒙古牛为普通牛型黄牛.观察统计了596个细胞的Ag-NORs数目,每细胞平均Ag—NORs数为5.818±0.513,变化范围为3~10个.  相似文献   

6.
采用外周血淋巴细胞培养及染色体分带技术分析了徐淮白山羊的染色体核型、C-带。结果表明,徐淮白山羊二倍体染色体数为2N=60,常染色体和X染色体均为端部着丝粒染色体.X染色体的大小介于1号和2号染色体之间,Y染色体最小.为中部着丝粒染色体,公羊核型为60.XY.母羊为60.XX。大部分常染色体和性染色体着丝粒部位显示阳性C带。  相似文献   

7.
不同类型苏丹草染色体数目及核型分析研究   总被引:2,自引:0,他引:2  
本文对苏丹草[Sorghumsudanense(Piper)Stapf]种内的黑壳(黑颖)、红壳(红颖)、白壳(白颖)3种类型进行染色体数目及核型研究,结果表明3种不同类的苏丹草染色体数目均为2n=20,核型公式均为Zn=4x=18m+2SAT,其染色体均为中部染色体,均具有一时带随体染色体,核型类型均为1A型,属于较对称性核型。苏丹草在种内其染色体及核型特征无明显差异。苏丹草与同属植物高梁的染色体、数目及核型特征有明显的属内一致性。  相似文献   

8.
对已封闭饲养40多年的AMS封闭群大鼠的正常核型,G带核型和生化金矿民性进行了研究。结果表明:该群大鼠染色体数目为42条,其中第2和第4-10条常染色体,Y染色体为末端着丝粒,第14条常染色体为中着丝粒,第1,3,11-13,15-20条染色体为亚中着丝粒,X染色体缺乏多形性,为单一的Xt型;染色体G带带型基本上与标准模式图相符。  相似文献   

9.
青海细毛羊染色体研究   总被引:2,自引:0,他引:2  
李军祥 《中国养羊》1996,16(1):8-13
采用外周血淋巴细胞培养及常规染色体标本制备技术,研究了青海细毛羊染色体的核型和畸变,并以GTG,CBG,硝酸银等方法对染色体标本进行分带处理,分别显示了青海细毛羊染色体的G-带,C-带和Ag-NORs带型,结果表明;(1)青海细毛羊二倍体细胞的染色体数目为2n=54,公羊核型式为54,XY,NF=62。母羊核型式为54,XX;NF=62。(2)染色体畸变类型主要为断裂和四倍体,频率分别为1.530  相似文献   

10.
采用外周血淋巴细胞培养方法,对洛阳地区八点黑獭兔的染色体核型与G-带带型进行分析。结果表明:洛阳八点黑獭兔的二倍体染色体数为2n=44,公兔的染色体核型为44,XY;母兔的染色体核型为44,XX。21对常染色体中,1~8号染色体为中着丝粒染色体,9~11号染色体为近中着丝粒染色体,12~17号染色体为近端着丝粒染色体,18~21号染色体为端着丝粒染色体。X染色体为近中着丝粒染色体,Y为最小的端着丝粒染色体。八点黑獭兔的带型基本上与其他家兔品系的染色体带型一致。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

17.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

18.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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