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1.
雏鸡感染柔嫩艾美尔球虫后鸡血清蛋白醋膜电泳分析 总被引：1，自引：0，他引：1
用 E.tenella卵囊感染雏鸡，通过醋酸纤维薄膜电泳检测 5种血清蛋白百分含量的变化。分别在感染后 3d和 7d进行采血检测。结果如下：试验组的蛋白 (％ )在感染后 7d显著低于对照组 (p<0.05）；试验组的α_1球司法部白（%）在感梁后7d比在3d显著下降（p<0.01）；试验组的γ球司法部白（%）在感染后7d极显著高于3d和对照组（p<0.01）。α_2球司法部白（%）在组间和组内无明显差异。 相似文献
2.
从广东部分地区采集鸭病毒性肝炎病毒 ,经半数致死量测定确定病毒毒力。取 1d无DHN病史、没有注射过DVH疫苗的健康仙湖三号鸭 2 0 0只 ,经 1周适应饲养后 ,随机分为对照组 (C组 ) ,弱毒株组 (S组 ) ,中毒株组 (M组 )以及强毒株组 (H组 ) 4个组 ,每组 5 0只。试验组背部肌肉分别注射 0 .3mL弱毒株、中毒株、强毒株的稀释液 ,对照组背部分点注射等量稀释液 ,隔离饲养。在攻毒后 1,3,5 ,7d采血 ,分别用形态学方法和酸性 -α-醋酸萘酯酶染色法 (ANAE)测定鸭外周血淋巴细胞转化率及ANAE阳性率。结果表明 :在整个试验期内 ,中毒组和强毒组的淋巴细胞转化率显著 (或极显著 )下降 (P <0 .0 5或P <0 .0 1) ,弱毒组仅在攻毒后 5d ,淋巴细胞转化率显著下降 (P <0 .0 5 ) ,其它时间与对照组相比没有显著差异 ;这些变化与病毒毒力有关 ;试验组在 3,5 ,7d测定时 ,与对照组相比 ,淋巴细胞转化升高 ,差异显著或极显著 (P <0 .0 5或P <0 .0 1) ,这些变化与病毒毒力无关。提示鸭病毒性肝炎病毒能抑制淋巴细胞的转化 ,这种抑制作用与毒力有关 ;攻毒组ANAE阳性率升高 ,不受病毒毒力强弱的影响。这些结果的不一致性 ,说明机体受到抗原侵袭时 ,免疫系统各组感受性发生的先后次序是不一致的 相似文献
3.
益微制剂对肉仔鸡生产性能及营养物质利用率的影响 总被引：2，自引：0，他引：2
试验应用内蒙古畜牧科学院研制的益微制剂———畜禽康乐宁 1# 饲喂肉鸡。饲养试验结果表明 ,添加畜禽康乐宁 1# 的试验组与黄霉素及空白对照组相比分别的提高日增重 1 36%和 7 0 3%(P >0 0 5 ) ,提高成活率为 3 4 5 %和 3 4 5 % (P >0 0 5 ) ,提高饲料转化率 3 73%和 10 39%。代谢试验结果表明 ,在育雏期 ,试验组和对照组之间在营养物质吸收上的差异均不显著 (P >0 0 5 )。在育成期 ,试验组与对照组间在粗脂肪、无氨浸出物、Ca、P的利用上差异不显著 (P >0 0 5 ) ;在粗蛋白的利用上 ,畜禽康乐宁 1# 组与黄霉素组差异不显著 (P >0 0 5 ) ,但显著高于对照组 (P <0 0 5 )。 相似文献
4.
蛋氨酸螯合锌对环颈雉雏雉生长的影响 总被引：1，自引：1，他引：0
随机选取 7日龄健康环颈雉雏雉 72只 ,分成 3组 ,每组 2 4只。每组基础日粮相同 ,Ⅰ组为对照组 ,添加 0 0 4 %硫酸锌 ;Ⅱ、Ⅲ组为试验组 ,分别添加 0 0 4 %蛋氨酸螯合锌、0 0 2 %蛋氨酸螯合锌 +0 0 2 %硫酸锌。饲养试验结果表明 ,各试验组体长、翅长增长均显著大于对照组 (P <0 0 5 ) ,各试验组平均日增重均极显著高于对照组 (P >0 0 1) ,同时各试验组料重比均低于对照组 (P <0 0 5 ) ,各试验组尾长增长也大于对照组 (P <0 0 5 )。此外 ,各试验组成活率均高于对照组。综合比较 ,Ⅱ、Ⅲ组饲喂效果好且无显著差异 (P >0 0 5 )。 相似文献
5.
随机选取 7日龄健雏 72只 ,分成 3组 ,每组 2 4只。每组基础日粮相同。 1组为对照组 ,添加 0 .0 4 %硫酸锌 ;2、3组为试验组 ,分别添加 0 .0 4 %蛋氨酸螯合锌、0 .0 2 %蛋氨酸螯合锌 +0 .0 2 %硫酸锌。饲养试验结果表明 ,各试验组平均日增重均极显著高于对照组 (P <0 .0 1) ,同时各试验组饲料转化率均高于对照组 (P >0 .0 5 ) ;各试验组体长、翅长增长均显著大于对照组 (P <0 .0 5 ) ,各试验组尾长增长也大于对照组 (P >0 .0 5 )。此外 ,各试验组成活率均高于对照组。综合比较 ,以 3组饲喂效果最好 相似文献
6.
日粮中添加复合酶制剂818A对蓝狐生长发育的影响 总被引：5，自引：1，他引：4
本文探讨了复合酶制剂 81 8A对幼龄蓝狐生长发育的影响。随机选取 50日龄左右蓝狐 80只 ,分成 5组 ,每组 1 6只。Ⅰ组为对照组 ,只饲喂基础日粮 ;Ⅱ、Ⅲ、Ⅳ、Ⅴ组为试验组 ,依次在基础日粮中添加 0 2 %、0 4 %、0 6%、0 8%的复合酶制剂 81 8A ,经 56d试验 ,结果表明 :各试验组狐平均日增重极显著高于对照组 (P <0 0 1 ) ,周体长增长值显著大于对照组 (P <0 0 5) ,同时料重比极显著低于对照组 (P <0 0 1 ) ,换毛时间也较对照组显著缩短 (P <0 0 5)。在各试验组中 ,Ⅲ、Ⅳ组生长较快 (P <0 0 5) ,Ⅳ、Ⅴ组病死率较低 (P <0 0 5)。 相似文献
7.
治疗剂量环丙沙星对育成猪免疫功能影响研究 总被引：2，自引：0，他引：2
为研究环丙沙星对免疫功能的影响 ,取 7周龄 ,体重 (2 5 5± 1 4 )kg ,长大杂交猪 12头 ,随机均分为试验组和对照组。试验组按每千克体重 5mg由耳静脉推注乳酸环丙沙星 ,每天 2次 ,连续 8d。施药第 0、7、14、2 1天 ,自前腔静脉采血 ,用显微镜法计数白细胞总数 ,姬姆萨染色法作白细胞分类计数 ,硝基蓝四氮唑 (NBT)还原试验定量检测嗜中性白细胞吞噬功能 ,噻唑蓝 (MTT)比色法检测外周血中淋巴细胞转化 ,单向免疫扩散法测定血清IgG浓度 ,5 0 %溶血试验测定血清中补体总活性 ,同时 ,用植物血凝素 (PHA)皮肤试验检测迟发型超敏 (DTH)反应。结果表明 ,环丙沙星对白细胞总数无影响 ,第 14天 ,试验组嗜中性白细胞百分含量显著低于对照组 (P <0 0 5 ) ,而淋巴细胞百分含量极显著升高 (P <0 0 1)。给药后 ,嗜中性白细胞还原硝基蓝四氮唑 (NBT)能力极显著升高 (P <0 0 1) ;植物血凝素 (PHA)刺激猪淋巴细胞体外增殖能力 ,第 7、14天极显著升高 (P <0 0 1) ,第 2 1天显著高于对照组 (P <0 0 5 ) ,PHA刺激迟发型皮肤超敏反应 (DTH)给药后第 7天极显著高于对照组 (P <0 0 1) ,而第 14、2 1天又显著低于对照组 (分别为P <0 0 5 ,P <0 0 1) ;给药后第 14天 ,血清中IgG的含量显著低于对照组 (P <0 0 5 ) ;第 7 相似文献
8.
选择年龄相近 ,健康无病 ,体重约 1kg的珍珠系长毛兔 1 0 0只 ,根据体重、性别 ,按组间相似的原则分为 4组 ,每组 2 5只 ,A、B、C 3组为试验组 ,分别添加 1 ,2 ,3号复合添加剂 ,D组为对照组 ,添加市售微量元素、多维素添加剂 ,蛋氨酸。试验各组基础日粮完全相同。60d的饲养试验结果表明 ,对照组的发病率显著高于试验组 ,而成活率则低于各试验组。B、C两组的平均增重显著高于对照组 (P <0 0 5 ) ;3个试验组相比 ,B、C组比A组增重分别提高1 0 34%和 1 4 94% ,差异显著 (P <0 0 5 ) ,而B、C两组间增重差异不显著 (P >0 0 5 )。 相似文献
9.
大豆黄酮对荷斯坦牛围产期血液生化成分的影响 总被引：4，自引：0，他引：4
12头初产乳荷斯坦牛 ,自身对照。试验组于预产期前 10d添喂大豆黄酮 80mg/d·头 ,在添喂后的第 7d、产后第 3,10和 17d采取血样 ,测定血浆中葡萄糖、尿素氮、谷丙转氨酶 (GPT)和胆固醇的含量。结果表明 :①血糖水平在添喂大豆黄酮后第 7d、产后第 3,10和 17d均显著低于 (P <0 .0 1)对照期 (饲喂前 ) ;②血浆总胆固醇在产后第 3d比对照组低 18.2 4 % (P <0 .0 5 ) ,而在产后第 17d时较对照高 34.2 2 % (P <0 .0 1) ;③尿素氮含量在添喂后第7d较对照高 14 .0 1% (P <0 .0 5 ) ,而在产后第 3,10d却比对照组低 14 .75 % (P <0 .0 5 )和 10 .10 % (P <0 .0 5 ) ;产后第17d略有回升 ;④GPT的活力于添喂大豆黄酮后第 7d较对照期高 71.31% (P <0 .0 1) ,在产后第 3d和 10d的GPT活力却显著低于 (P <0 .0 5 )对照期 ,分别为 14 .98%和 9.4 8% ,至第 17d却高于对照 8.74 %。以上结果提示 :大豆黄酮能影响乳牛围产期的糖、脂和蛋白质代谢 ,促进了营养物质的转化 ,加强了机体蛋白质的合成 ,以适应机体泌乳的生理变化 相似文献
10.
不同酶制剂组合在环颈雉育雏中的应用效果 总被引：1，自引：0，他引：1
研究酶制剂的 3种组合作为饲料添加剂 ,在环颈雉育雏中的应用效果。Ⅰ组为对照组 ,仅饲喂基础日粮 ;Ⅱ、Ⅲ、Ⅳ组为试验组 ,分别在基础日粮中添加 0 5 %纤维素酶 +0 5 %中性蛋白酶 ,0 3%纤维素酶 +0 3%淀粉酶 +0 4 %中性蛋白酶 ,0 2 %纤维素酶 +0 2 %淀粉酶 +0 2 %植酸酶 +0 4 %中性蛋白酶。经 2 1d试验 ,结果表明 ,Ⅱ组平均日增重显著高于Ⅰ组 (P <0 0 5 ) ,Ⅲ、Ⅳ组平均日增重极显著高于Ⅰ组 (P <0 0 1) ;同时各试验组料重比均低于Ⅰ组 ,且Ⅲ、Ⅳ组达显著水平 (P <0 0 5 ) ;各试验组体尺 (体长、翅长、尾长 )增长也大于Ⅰ组 ,且Ⅲ、Ⅳ组达显著水平 (P <0 0 5 )。此外 ,各试验组成活率明显高于Ⅰ组。在试验组中进行综合比较 ,以Ⅳ组应用效果最好 相似文献
11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。 相似文献
12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections
of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal
(GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April
2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs
located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming
areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology
laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum
columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying
degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal
abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy
season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different
nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those
observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes.
But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the
results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia. 相似文献
13.
Tropical Animal Health and Production - 相似文献
14.
Rassnick KM Gieger TL Williams LE Ruslander DM Northrup NC Kristal O Myers NC Moore AS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):196-199
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks. 相似文献
15.
H Hayashi T Yoshimura J Y Chen 《Comparative immunology, microbiology and infectious diseases》1985,8(2):73-87
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed. 相似文献
16.
Davis MS Willard MD Nelson SL Mandsager RE McKiernan BS Mansell JK Lehenbauer TW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(3):311-314
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large. 相似文献
17.
Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects 总被引：1，自引：0，他引：1
Johansson AM Gardner SY Levine JF Papich MG LaFevers DH Fuquay LR Reagan VH Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses. 相似文献
18.
B. Cinar Kul N. Bilgen J.A. Lenstra O. Korkmaz Agaoglu B. Akyuz O. Ertugrul 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):449-453
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded. 相似文献
19.
Ling GV Thurmond MC Choi YK Franti CE Ruby AL Johnson DL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):817-823
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors. 相似文献
20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems. 相似文献