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1.
对蕙兰TUB基因片段进行克隆与序列分析,为蕙兰内参基因的筛选和研究其生理功能提供研究基础。根据NCBI已经公布的TUB基因的同源核苷酸保守序列,进行简并引物设计,利用RT-PCR的方法,以蕙兰花蕾期的花葶为材料,克隆了5条TUB基因片段。序列分析结果表明,5条TUB基因片段长度均为1055bp,编码351个氨基酸,具有2个TUB基因标记位点：Tubulin-betamRNAautoregulationsignal：MREI和Tubulinsubunitsalpha,beta,andgammasignature(GGGTGSG)特征信号序列。各片段之间同源性高达91.72%,经BLAST分析,与小麦的TUB1同源性可达85%。利用DNAMAN等生物软件推断的氨基酸,与毛果杨、蓖麻、葡萄同源性达97%。研究中得到的5个基因序列是TUB基因的同源片段,分别命名为CfTUB1、CfTUB2、CfTUB3、CfTUB4和CfTUB5,并在GenBank注册,登录号分为JN177713、JN177714、JN177715、JN177716和JN177717。  相似文献   

2.
为研究脱水素在植物早期胚胎发育中的作用和机理,采用显微操作技术获得烟草卵细胞并构建其c DNA文库,从中筛选到一个脱水素基因NtDEH1。通过RACE技术获得该基因全长,基因组测序结合生物信息学分析表明该基因拥有一段长度为651 bp的完整开放阅读框和一段535 bp的内含子,编码由217个氨基酸残基组成的蛋白质,其理论等电点为5.27,属于酸性蛋白,且具有一定的亲水性。氨基酸序列比对分析发现在许多物种比如绒毛状烟草、林烟草、甜辣椒、番茄、马铃薯和丹参中都具有与NtDEH1蛋白非常类似的保守的同源序列,均具有SK2型脱水素特征。借助Genome walking技术获取NtDEH1基因约1 706 bp的5'侧翼序列,使用小叶烟草瞬时表达系统检测到其具有较强的启动子活性。该研究为进一步了解脱水素基因NtDEH1在植物生殖发育中的具体功能打下基础。  相似文献   

3.
香味基因是香稻育种的关键。本研究采用香味基因Xiang7F/7R对来源于辽宁省农科院和牡丹江市场的9个水稻品种进行序列分析。结果表明:(1)9个水稻品种在近400 bp处均出现一条带。(2)序列同源性分析发现,稼禾1号和龙粳香1号出现8 bp的片段缺失和3 bp的碱基突变。(3)日本晴与NCBI上发布的日本晴在该基因位点上存在5个碱基的插入/缺失,同源相似性达到99%。(4)遗传距离和系统发育树均表明,稼禾1号和龙粳香1号的遗传距离为0,聚为同一分支。以上结果表明,香味基因对不同水稻品种具有较高的灵敏度,可用于香稻品种的筛选,进而为后续的香稻品种选育奠定基础。  相似文献   

4.
以牡丹基因组为模板,利用简并引物进行PCR扩增,得到了编码Ty1-copia类反转录转座子反转录酶(RT基因)的序列,测序后所得序列长度为240 bp,包含2个开放读码框,共编码63个氨基酸。经NCBI中BLAST比对结果显示,洛阳红牡丹RT基因与枸杞、甜菜等RT基因的同源性均在70%以上。经MEGA和DNAstar软件分析表明,该片段在进化上与梅、杨、茶和苹果等落叶木本植物的同源性序列存在更高的同源性和更近的亲缘关系,与枸杞、鹰嘴豆、番茄、草莓等草本植物的同源性序列亲缘性较远。  相似文献   

5.
为了给筛选萼脊兰内参基因和研究TUB基因的生理功能提供研究基础,对萼脊兰TUB基因片段进行克隆与序列分析。采用CTAB法提取萼脊兰盛花期花瓣总RNA,并运用RT-PCR方法克隆萼脊兰TUB基因序列,长度为1 055 bp,编码351个氨基酸残基,氨基酸序列中存在微管蛋白的保守区域GGGTGSG。序列分析结果表明:与其他已登录物种的TUB基因相比,其核苷酸序列的同源性达75%~96%,与朵丽蝶兰(JN185665.1)同源性高达96%,氨基酸序列的同源性也在96%以上,且与单子叶植物的同源序列表现出较高的相似性。在Gen Bank注册,登录号为KP686397。  相似文献   

6.
胡桃楸成花相关LFY同源基因的克隆和序列分析   总被引：1，自引：0，他引：1  
通过对胡桃楸成花相关LFY基因进行研究,对缩短胡桃楸童期,促进其提早开花结实有着极为重要的意义。采取PCR扩增技术,在NCBI数据库中进行搜索,查找到与目的基因有关联的同源序列;并根据这些有关联的序列来进行引物设计,扩增目的基因全长。克隆LFY基因cDNA序列全长为1 297 bp,其中CDS序列长1 158 bp,编码385个氨基酸。NCBI数据库Blast比对表明胡桃楸LFY同源基因与其他物种的LFY同源基因同源性均在80%以上。将胡桃楸LFY同源基因命名为JmLFY(GenBank登录号:KX364241)。通过BioEdit软件和ProtParam ExPASy在线软件分析胡桃楸JmLFY蛋白质分子式为C_(1878)H_(2962)N_(566)O_(578)S_(13),分子量为43 134.4 Da;理论等电点pI值为6.78;不稳定指数为39.92,属于不稳定蛋白质。该研究为进一步探索LFY基因对胡桃楸成花的影响提供依据。  相似文献   

7.
MADS-box基因家族是一类具有特异性序列的转录因子,并在植物体发育过程中起着重要作用。本研究以毛白杨形成层为材料,利用RACE技术克隆得到4个MADS-box基因,命名为Pt MADS1、Pt MADS2、Pt MADS3和Pt MADS5。Pt MADS1全长1 067 bp,开放阅读框(ORF)长度678 bp,编码含有225个氨基酸;Pt MADS2全长912 bp,ORF长度609 bp,编码含有202个氨基酸;Pt MADS3全长909 bp,ORF长度654 bp,编码含有217个氨基酸;Pt MADS5全长1 029 bp,ORF长度663 bp,编码220个氨基酸。系统进化树分析表明Pt MADS1与SVP同源性较高,属于STMADS11亚族;Pt MADS2与AGL12基因同源性较高,属于AGL12亚族;Pt MADS3和Pt MADS5分别与AGL14和SOC1同源性较高,同属于SOC1亚族。  相似文献   

8.
《分子植物育种》2021,19(8):2521-2526
为了获得姜荷花叶绿素降解的关键酶基因PPH信息,本试验在前期通过姜荷花全长转录组测序获得大量转录组信息的基础上,进行筛选分析,获得了2条PPH基因,分别命名为PPH1和PPH2。PPH1基因(GenBank:MT077178),cDNA序列全长1 795 bp,开放阅读框1 437 bp (138～1 574 bp),编码一条478 aa的氨基酸序列。PPH2基因(GenBank:MT077179),c DNA序列全长1 393 bp,开放阅读框1 227 bp (70～1 296 bp),编码一条408 aa的氨基酸序列。采用BLAST、Translate tool (ExPASy)、Clustal Omega、Find Conserved Domains(NCBI)、ProtParam、TMHMM Server、SOPMA、SWISS-MODEL、ClustalX (1.81)、MEGA 4.1等预测和分析了这2个基因编码蛋白氨基酸的一级结构(包括氨基酸序列的组成,保守区,理化特征等)、二级结构、三级结构及分子系统进化关系。PPH1和PPH2的核苷酸与蛋白氨基酸序列与其它物种的PPH基因都具有很高的同源性,两者都包含有水解酶(Hydrolase)特征PLN02578的保守区。分子系统进化分析表明,姜荷花的PPH1和PPH2聚为一小类,然后与小果野芭蕉PPH (XP_018677219.1)的亲缘关系最为接近,而与双子叶植物的关系较远。本研究为后期通过遗传转化手段改良姜荷花不育苞片的颜色提供了分子基础。  相似文献   

9.
柽柳MnSOD基因的克隆及功能验证   总被引：2，自引：0，他引：2  
根据已克隆得到的柽柳MnSOD基因片段,采用RACE技术从柽柳中获得MnSOD的cDNA全长序列.该基因全长为1 087 bp.其中,5'非编码区40 bp,3'非编码区333 bp,开放读码框(ORF)长699 bp,编码232个氨基酸.分子量为26 KD,等电点为7.10.该MnSOD氨基酸序列与拟南芥MnSOD序列同源性最高为83%,从12个物种的序列比对可以看出MnSOD氨基酸序列保守性较高.该序列的Genbank注册号为AY573576(基因)和AAS77885(蛋白).将MnSOD基因与酵母表达载体pYES2连接,并进一步转化到酵母基因组中.对重组酵母INVSc1(pYES2-SOD)和对照INVSc1 (pYES2)进行干旱、高温胁迫实验.结果表明:重组酵母的抗干旱、高温能力明显高于对照,证实了来源于柽柳的MnSOD基因具有抗干旱和耐高温能力.  相似文献   

10.
GGP(GDP-L-galactose phosphorylase)参与植物AsA合成过程。本研究以甜樱桃‘佐藤锦’(Satonishiki)为材料,采用PCR法,首次在甜樱桃上克隆了AsA合成相关基因PacGGP1和PacGGP2,基因片段长度分别为1 297 bp和1 765 bp,分别包含1 107 bp和1 341 bp,并分别编码368和446个氨基酸。通过氨基酸序列比对结果发现,与甜樱桃PacGGP1同源性较高的是枣GGP1,相似度100%,与甜樱桃PacGGP2同源性较高的是碧桃GGP2,相似度在98%左右。采用RT-PCR方法分析了PacGGP1和PacGGP2的表达模式。研究表明PacGGP2基因在‘佐藤锦’甜樱桃品种中的表达变化模式与T-AsA的含量变化趋势表现出明显的相似性,推测它可能是AsA L-半乳糖合成途径中的关键基因,可为甜樱桃分子育种奠定重要基础。  相似文献   

11.
Autotoxicity restricts reseeding of alfalfa (Medicago sativa L.) after alfalfa until autotoxic chemical(s) breaks down or is dispersed into external environments. A series of aqueous extracts from leaves, stems, roots and seeds of alfalfa ‘Vernal’ were bioassayed against alfalfa seedlings of the same cultivar to determine their autotoxicity. The highest inhibition was found in the extracts from the leaves. Extracts at 40 g dry tissue l?1 from alfalfa leaves were 15.4, 17.5 and 28.7 times more toxic to alfalfa root growth than were those from roots, stems and seeds, respectively. A high‐performance liquid chromatography (HPLC) analysis with nine standard compounds showed that the concentrations and compositions of allelopathic compounds depended on the plant parts. In leaf extracts that showed the most inhibitory effect on root growth, the highest amounts of allelochemicals were detected. Among nine phenolic compounds assayed for their phytotoxicity on root growth of alfalfa, coumarin, trans‐cinnamic acid and o‐coumaric acid at 10?3 m were most inhibitory. The type and amount of causative allelochemicals found in alfalfa plant parts were highly correlated with the results of the bioassay, indicating that the autotoxic effects of alfalfa plant parts significantly differed.  相似文献   

12.
Development of onion (Allium cepa L., cv. ‘Early Cream Gold’) seed under cool climate conditions in Tasmania, Australia occurred over a longer duration than previously reported, but similar patterns of change in yield components were recorded. In contrast to previous studies, umbel moisture content declined from 85 to 67 % over 57 days while seed moisture content decreased from 85 to 31 %. Seed yield continued to increase over the duration of crop development, with increasing seed weight compensating for seed loss resulting from capsule dehiscence in the later stages of maturation. Germination percentage was high and did not vary significantly from 53 to 77 days after full bloom (DAF), but mean germination time declined and uniformity of germination increased significantly over the same time period. The percentage abnormal seedlings declined with later harvest date, resulting in highest seed quality at 77 DAF. The results of this study suggest that the decision to harvest cool climate onion seed crops before capsule dehiscence will result in a loss of potential seed yield and quality.  相似文献   

13.
Jens Jensen 《Euphytica》1979,28(1):47-56
Summary The high-lysine gene in Risø mutant 1508 conditions an increased lysine content in the endosperm via a changed protein composition, a decreased seed size, and several other characters of the seed. The designation lys3a, lys3b, and lys3c, is proposed for the allelic high-lysine genes in three Risø mutants, nos 1508, 18, and 19. Linkage studies with translocations locate the lys3 locus in the centromere region of chromosome 7. A linkage study involving the loci lys3 and ddt (resistance to DDT) together with the marker loci fs (fragile stem), s (short rachilla hairs), and r (smooth awn) show that the order of the five loci on chromosome 7 from the long to the short chromosome arm is r, s, fs, lys3, ddt. The distance from locus r to locus ddt is about 100 centimorgans.  相似文献   

14.
G. H. Kroon 《Euphytica》1994,76(1-2):125-125
Summary K x vadensis is a hybrid of K. blossfeldiana and K. marmorata obtained after doubling the number of chromosomes.  相似文献   

15.
[Objectives]This study aimed to establish a QAMS(quantitative analysis of multi-components by single-marker)method for simultaneous determination of four phenol...  相似文献   

16.
Summary Avoidance of rust fungi that was based on poor appressorium induction was previously found in Hordeum chilense. In the present study 95 accessions of Triticeae were screened for avoidance of Puccinia hordei. The percentage of appressorium formation per germinated spore ranged from 6 to 90%. On none of the 41 accessions of Aegilops, Agropyron, Elymus, Secale, Thinopyrum or Triticum studied was the rate of appressorium formation lower than 25%. Lower rates of appressorium formation were, however, found on accessions of wild barley species Hordeum brachyantherum, H. marinum, H. parodii and H. secalinum. Its implications in cereal breeding are discussed.  相似文献   

17.
P. A. York  R. Cook 《Euphytica》1989,43(1-2):135-141
Summary Reactions of 13 grasses to Meloidogyne naasi varied with species; ryegrasses, fescues and their hybrids were generally susceptible and cocksfoot and timothy resistant. Marked variation in host resistance levels occurred between genotypes within cultivars.Selection of single plants, followed by tests on replicate tillers, identified resistant and susceptible genotypes in both Italian and perennial ryegrass cultivars. Resistant plants had few nematode-induced galls and fewer females and eggs than susceptibles. There was more or less continuous variation, with many genotypes intermediate between extremes of resistance and susceptibility. Selected resistant and susceptible genotypes are of use in assessing variation in nematode populations and as controls for breeding and selection programmes.  相似文献   

18.
Lentil production is limited by lack of moisture and unfavorable temperatures throughout its distribution. Waterlogging and salinity are only locally important. Progress has been made in breeding for tolerance to drought through selection for an appropriate phenology and increased water use efficiency and in breeding for winter hardiness through selection for cold tolerance.The diseases rust, vascular wilt, and Ascochyta blight, caused by Uromyces viciae-fabae, Fusarium oxysporum f. sp. lentis, and Ascochyta fabae f. sp. lentis, respectively, are the key fungal pathogens of lentil. Cultivars with resistance to rust and Ascochyta blight have been released in several countries and resistant sources to vascular wilt are being exploited. Sources of resistance to several other fungal and viral diseases of regional importance are known. In contrast, although the pea leaf weevil (Sitona spp.) and the parasitic weed broomrape (Orobanche spp.), and to a lesser extent the cyst nematode (Heterodera ciceri), are significant yield reducers of lentil, no sources of resistance to these biotic stresses have been found. Directions for future research in lentil on both biotic and abiotic stresses are discussed.  相似文献   

19.
Progress is being made, mainly by ICARDA but also elsewhere, in breeding for resistance to Botrytis, AScochyta, Uromyces, and Orobanche; and some lines have resistance to more than one pathogen. The strategy is to extend multiple resistance but also to seek new and durable forms of resistance. Internationally coordinated programs are needed to maintain the momentum of this work.Tolerance of abiotic stresses leads to types suited to dry or cold environments rather than broad adaptability, but in this cross-pollinated species, the more hybrid vigor expressed by a cultivar, the more it is likely to tolerate various stresses.  相似文献   

20.
[Objectives]To optimize the water extraction process of Chinese Herbal Compound Man Gan Ning and establish a method for its extraction and content determination...  相似文献   

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