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1.
内蒙古绒山羊KAP基因与经济性状关系的研究   总被引：6，自引：0，他引：6  
以60只内蒙古绒山羊为研究对象,利用PCR-SSCP技术,选取KAP基因作为候选基因,并利用SPSS软件下的GLM程序分析了KAP基因与产绒量性状、体重性状和绒细度性状的关系。结果表明：在产绒量性状上,S1位点的BB基因型和S2位点的BB基因型可以作为标记基因型;在体重性状上,S2位点的BB基因型和S3位点的AA基因型可以作为标记基因型,在绒细度性状上,S5位点的BB基因型可以作为标记基因型。在体重和产绒量性状上S2位点的BB基因型,可以作为多性状标记辅助选择的标记基因型。  相似文献   

2.
根据山羊遗传连锁图谱和相关资料,选用7个微卫星座位,对40只辽宁绒山羊基因组DNA的遗传多样性进行检测;通过标记多态性与生产性状的最小二乘线性模型,进行分子标记与经济性状的相关分析.结果表明,7个微卫星座位中只有4个表现出了多态性.微卫星OarJMP8座位上AA、AD基因型个体羊绒纤维长度显著高于其它基因型个体(P<0.05),AD基因型个体产绒量显著高于其它基因型个体(P<0.05);微卫星BM143座位上AC基因型个体羊绒纤维细度显著低于其它基因型个体(P<0.05);微卫星OarAE101座位上AB基因型个体羊绒纤维长度和产绒量均显著或极显著高于其它基因型个体(P<0.05,P<0.01);微卫星BM6506座位上从基因型个体产绒量极显著高于其它基因型个体(P<0.01).  相似文献   

3.
研究分析了辽宁绒山羊3个经济性状的相关性.根据山羊遗传连锁图谱和相关资料,选用7个微卫星座位,对40只辽宁绒山羊基因组DNA的遗传多样性进行检测,通过标记多态性与生产性状的最小二乘线性模型,进行分子标记与经济性状的相关分析.结果表明:7个微卫星座位中只有4个表现出了多态性.微卫星OarJMP8座位上AA、AD基因型个体羊绒纤维长度极显著高于其他基因型个体(P＜0.01),AD基因型个体产绒量显著高于其他基因型个体(P＜0.05);微卫星BM143座位上AC基因型个体羊绒纤维细度显著低于其他基因型个体(P＜0.05);微卫星OarAE101座位上AB基因型个体羊绒纤维长度和产绒量均显著高于其他基因型个体(P＜0.05);微卫星BM6506座位上从基因型个体产绒量极显著高于其它基因型个体(P＜0.01).  相似文献   

4.
为了进一步培育优质高产陇东绒山羊群体,利用引进的绒细型和绒长型种公羊进行分区域杂交改良和品系繁育,培育出适宜陇东干旱半干旱自然生态条件的陇东绒山羊专门化品系。培育结果表明:绒细型特一级成年公母羊平均产绒量分别为601.67g和465.76g,体重分别为36.16kg和29.31kg,羊绒细度分别为14.49μm和14.25μm;绒长型特一级成年公母羊平均产绒量分别为626.49g和506.37g,体重分别为37.74kg和29.65kg,羊绒细度分别为15.02μm和14.82μm。同时利用微卫星DNA技术研究了9对基因座位在陇东绒山羊群体中的遗传多样性,通过标记多态性与生产性状的最小二乘线性模型,进行了分子标记与经济性状的相关分析,并在这些位点上找到经济性状优势基因型以及对相应性状具有正效应或负效应的等位基因。  相似文献   

5.
作者研究了7个微卫星标记在2个南疆绒山羊群体中的遗传多样性,并用SAS中GLM过程分析了抓绒量、抓绒体重、绒纤维平均直径、绒纤维直径变异系数、净绒率和绒丛长度6个经济性状的标记效应及群效应。结果表明,在南疆绒山羊群体中,等位基因数为4～8个,多态信息含量为0.3186～0.7930,杂合度为0.3344～0.8175。抓绒体重和净绒率存在群效应,BMS 2782位点的A、C、I三个基因型和IDVGA 64位点的B基因型为绒丛长度的优势基因型,LSCV 13位点的B基因型为产绒量的优势基因型。  相似文献   

6.
《畜牧与兽医》2015,(9):48-51
以陇东绒山羊为研究对象,选择生长激素(GH)基因作为候选基因,采用PCR-SSCP分子标记技术和DNA测序技术检测GH基因多态性,然后运用最小二乘方差分析法对不同基因型间生长发育性状及产绒性状进行显著性检验。结果表明:在GH基因外显子4区域1 450 bp处发生了C→T的单碱基突变;该多态位点对产绒性状无显著影响(P0.05),而对部分体重、体尺指标有显著影响(P0.05);6月龄重、周岁重、胸围等指标,HH型显著高于GG型和GH型(P0.05)。由此推测:HH型可以作为陇东绒山羊早期生长发育性状辅助选择的标记基因型。  相似文献   

7.
《畜牧与兽医》2020,(2):13-17
为分析西藏绒山羊角蛋白关联蛋白(KAP)7.1基因和8.2基因与生产性状的关系,采用PCR-SSCP和DNA测序技术对340只西藏绒山羊KAP7.1基因和KAP8.2基因编码区序列进行多态性分析,并与体长、体高、胸围、产绒量、羊绒长度、羊毛长度等生产性状进行关联分析。结果显示:PCR-SSCP检测发现KAP7.1基因只有1种带型,KAP8.2基因有6种带型;KAP7.1基因扩增产物序列中未发现碱基变化;KAP8.2基因扩增产物序列中存在5个SNPs位点,分别为C115T、A207G、A214G、G216A和A278T,除了A278T外都位于编码区;对KAP8.2基因的基因型1、基因型2和基因型3与生产性能进行关联分析发现,基因型1个体的胸深和产绒量显著高于基因型3个体(P<0.05)。结果表明:KAP8.2基因可作为影响西藏绒山羊胸深和产绒量的分子辅助选择标记。  相似文献   

8.
为了找出与绍兴鸭开产体重有协同作用的微卫星遗传标记,对绍兴鸭群体中215个个体的16个微卫星基因座位的基因型进行分析,所有基因座位都存在多态性。并对所有基因座位的基因型与开产体重进行组分方差分析和Bayesian后验概率分析。结果表明:在组分方差分析中,微卫星AY493294基因型与开产体重有极显著(P<0.01)的协同关系;Bayesian后验概率分析结果表明,该位点重新判型后的基因型CC(即等位基因长度在200~300 bp的纯合型)对开产体重的后验概率达到了极显著(P=0.998)。以上结果证明,AY493294可以作为绍兴鸭开产体重的一个辅助选择的遗传标记。  相似文献   

9.
辽宁绒山羊主要经济性状的典型相关分析   总被引：1，自引：0，他引：1  
本研究取材于辽宁省绒山羊原种场的114只成年母羊3年的育种资料，确定了产绒量、细度、长度、净绒率、净绒量、体重等6个经济性状，划分为3组，即产量性状、绒品质性状和体重性状，运用典型相关分析方法，对3组性状间的关系进行了研究，同时，对典型相关与简单相关进行了比较。结果表明，产量性状与品质性状 体重性状的相关是由净绒量与长度、净绒量与细度、净绒率与长度相关引起的；产量性状与品质性状的相关是由净绒量与细度和产绒量与长度相关引起的；产量性状与体重性状的相关是由产绒量与体重相关引起的。典型相关揭示出性状间的相关实质，更能综合地反映两组变量间的整体相关关系。  相似文献   

10.
微卫星标记与丝羽乌骨鸡产蛋性能的关系研究   总被引：8，自引：0，他引：8  
从鸡的遗传图谱中选择9个与产蛋性能相关的微卫星基因座,对在产蛋性能上有明显差异的丝羽乌骨鸡个体共170只进行标记基因型与产蛋性状的最小二乘分析,结果表明：有4个标记G31913、X82867、Z95315、G01672与丝羽乌骨鸡的4个月连产蛋量、开产蛋重及500日龄产蛋量间存在显著的关系。标记G31913中基因型AB所对应的开产体重最小二乘均值与其它基因型的对应值间的差异达显著或极显著,开产蛋重间无显著差异。标记Z95315的基因型CC所对应开产蛋重、开产体重最小二乘均值最高,并且均显著高于其余8种基因型所对应的开产蛋重、开产体重最小二乘均值。标记G01672基因型为EE、AE所对应开产蛋重最小二乘均值与基因型为CE、CC的对应值间差异显著;等位基因E与开产蛋重有显著正相关,等位基因C与开产蛋重有显著负相关。标记X82867基因型CD所对应500日龄产蛋量最小二乘均值显著低于AB型,4个月连产蛋量、300日龄产蛋量也低于基因型为AB个体。研究表明,标记G31913的基因型AB和标记Z95315的基因型CC有望作为开产蛋重、开产体重早期选择的辅助标记。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
Tropical Animal Health and Production -  相似文献   

14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

17.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

18.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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