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1.
用新型SPA协同凝集(SPA-CoA)试验对144份猪血清进行了乙型脑炎病毒(JEV)抗体检测,并与血凝抑制试验(HI)进行了对比。2种方法检测结果,血凝抑制试验检测阳性率为57.63%(83/144),新型SPA-CoA阳性率为63.89%(92/144);二者阳性符合率87.00%(80/92),总符合率为88.89%(128/144)。对8份SPF猪血清进行检测,2种方法检测结果均为阴性。结果表明,新型SPA-CoA与HI检测乙型脑炎结果符合,前者更为简便和实用。  相似文献   

2.
猪乙型脑炎乳胶凝集试验与血凝抑制试验方法的比较   总被引：2，自引：0，他引：2  
用血凝抑制试验 (HI)和乳胶凝集试验 (LAT)两种方法检测乙型脑炎弱毒疫苗免疫猪血清 ,结果均呈阳性反应。用LAT对来自 12个猪场的 94份猪血清进行了乙型脑炎病毒 (JEV)抗体检测 ,并与HI进行了对比 ,两种方法检测结果阳性符合率和总符合率分别为 90 .3% (5 6 /6 2 )和 88.3% (83/94 ) ,两种方法检测结果差异不显著 (p >0 .0 5 )。对来自无乙型脑炎的 12头健康猪血清进行检测 ,两种方法检测结果均为阴性。结果表明 ,用LAT与HI检测乙型脑炎结果符合 ,前者更为简便和实用。  相似文献   

3.
分别采用商品化的酶联免疫吸附试验(ELISA)抗体检测试剂盒和血凝抑制(HI)试验方法对153份血清样本进行禽流感病毒(AIV)的H5抗体检测,比较间接ELISA和HI两种方法检测结果的相关性。研究结果表明,间接ELISA和HI两种方法呈较好的相关性,二者间的相关系数为0.765。间接ELISA和HI两种方法检测AIV抗体的阳性符合率为95.8%,阴性符合率为87.2%,总体符合率为96.7%。间接ELISA方法是一种敏感、特异和快速的血清学诊断方法,可以用于临床样本的大规模检测。  相似文献   

4.
用血凝抑制试验和间接ELISA两种方法检测乙脑阳性血清均呈阳性反应,检测乙脑阴性血清均呈阴性反应。用间接ELISA对来自9个猪场74份送检血清进行了猪乙脑病毒（Japanese encephalitis virus,JEV）抗体检测,并与血凝抑制试验（HI）进行对比,两种方法检测总符合率为85.1%(63/74),经统计分析,检测结果差异不显著(P＞0.05)。对来自无猪乙脑病猪场24头健康猪的血清进行检测,两种方法结果均为阴性,符合率为100%。对10份阳性血清进行检测,ELISA检测效价较HI效价高,表明ELISA比HI敏感。结果表明,ELISA与HI检测结果相符,前者更适用于猪血清中乙脑IgG抗体的大规模检测。  相似文献   

5.
用哈尔滨维科生物技术开发公司生产的批号为200901的鸡减蛋综合征京911株油乳剂灭活疫苗,以0.5mL/只剂量接种120日龄SPF鸡10只,7d后采血分离血清,以后每隔一定时间进行采血、分离血清。同时又采集了发病鸡场的15份发病鸡血清。分别用血凝抑制试验和琼脂扩散(AGP)试验对自制的阳性血清和发病鸡血清样品进行血清学检查。结果表明:当血清HI效价等于或大于28时,两种方法的符合率可达100%;当血清的HI效价为26~27时,AGP与HI的符合率为73.33%~86.67%;当血清的HI效价为24~25时AGP的检出率很低或完全呈阴性。从两种方法的符合率比较还可得出HI试验检测的灵敏度要高于AGP,而且HI法简单、特异、快速、实用、方便,不但能定性还可以定量。  相似文献   

6.
为评估快速胶体金免疫层析条(colloidal gold imunochromatography assay,GICA)诊断现场家畜日本血吸虫病的价值,以GICA法对来自湖南血吸虫病流行区和非流行区的山羊、水牛、黄牛血清进行栓测,并和间接血凝法(indirect haemagglutination test,IHA)及粪便孵化法的诊断结果进行比较.结果显示,GICA对流行区284只山羊、172头水牛和145头黄牛血清检测,阳性率分别为10.21%、8.14%、8.28%;对非流行区的30只山羊、25头水牛、17头黄牛检测,假阳性率分别为10%、12%和11.76%.GICA和IHA的诊断结果相比,阳性符合率分别为93.8%、100%、100%,阴性符合率分别为99.7%、98.9%、98.7%;GICA与粪便孵化法的诊断结果相比,阳性符合率均为100%,阴性符合率分别为94.6%、96.9%、94.3%.可见,GICA法快速、简便,可以代替现行的IHA和粪便孵化检查法,用于疫区家畜血吸虫痛的筛查.  相似文献   

7.
冻干(醛化)红细胞和新鲜红细胞与同一份鸡血清分别做鸡新城疫(ND)的血凝(HA)和血凝抑制(HI)试验,二者血凝抑制价基本符合率达98.72%,相关系数为0.9288.冻干红细胞完全可以代替新鲜红细胞做血凝抑制试验检测鸡新城疫抗体效价.  相似文献   

8.
在32个月期间对10个牛群的14个月以上小母牛及成年母牛同时进行粪便细菌学培养和免疫琼扩(AGID)试验,以确定AGID试验诊断亚临床型牛副结核的效果。 在139头牛的粪样中有109头分离到副结核分枝杆菌,同时有28.8%(40/139)为AGID阳性,而在上述109头中有36头为AGAD阳性(33%)。如将屠宰时的结果包括在内,则培养法诊断为感染的117头,其中55头(47%)曾为AGID阳性。AGID假阳性最高上限为2.1%。 据培养的菌落数,AGID阳性的亚临床型感染牛粪中排菌数多(P<0.0001),对感染牛进行重复的细菌培养和AGID试验,均缺乏一致的阳性重复。 48头在犊牛期经副结核分枝杆菌菌苗免疫过的成年牛,有3头为AGID阳性,从其中1头粪便分离到副结核分枝杆菌,这表明在犊牛期免疫过的牛,其AGID假阳性率是低的。 程安春 摘译自 Am J Vet Res,1989,50(4):525～530 汪铭书校  相似文献   

9.
三抗体间接Dot-ELISA检测犬旋毛虫病IgG抗体的研究   总被引：3，自引：0，他引：3  
本试验成功地建立了检测旋毛虫病犬血清中的特异性抗体的一种新的免疫学诊断法 -三抗体间接 Dot-EL ISA法 ,并与压片镜检法 (TSM)和琼脂扩散试验 (AGID)进行了比较。试验结果表明 ,Dot- EL ISA和 AGID对2 4 5份被检血清的阳性检出率为 2 2 .86 % (5 6 / 2 4 5 )和 6 .94 % (17/ 2 4 5 ) ,两者符合为 30 .36 %。该法的检出率比AGID高 15 .92 % ,比 TSM高 18.37%。Dot- EL ISA的相对灵敏度为 AGID的 6 7.4 7倍  相似文献   

10.
首先应用血凝抑制试验(HI)对从山东省部分地区采集的178份猪血清样品进行了猪血凝性脑脊髓炎病毒(HEV)抗体检测。结果显示,这些猪群中HEV的HI抗体总体阳性率高达61.24%(109/178),说明HEV的感染较为普遍。同时,以纯化的病毒作为包被抗原,通过优化反应条件,成功建立了HEV抗体间接酶联免疫吸附试验(ELISA)检测方法。应用该ELISA方法对从山东地区猪血清中随机抽取的44份样品进行检测,结果显示,HEV抗体阳性率为72.73%(32/44);而HI抗体阳性率为56.82%(25/44)。两种检测方法的阳性符合率为92.00%。结果表明,建立的ELISA方法较HI方法敏感性高。  相似文献   

11.
Hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) tests were compared to the serum neutralization (SN) test to evaluate their ability to detect antibodies to encephalomyocarditis virus (EMCV). Swine fetal thoracic fluids of known EMCV SN antibody titers (200 samples greater than or equal to 1:2, 100 samples less than 1:2) were selected from a collection of field cases. The thoracic fluids were tested for EMCV antibodies by HI and AGID, and the results were compared to those of the SN test. Of 200 SN antibody-positive samples, 183 (91.5%) and 173 (86.5%) were positive in HI and AGID tests, respectively. Of 100 SN-negative samples, 81 (81%) and 94 (94%) were negative in HI and AGID tests, respectively. Agreement between the tests was analyzed by calculating Kappa values. The values were 0.73 between SN and HI tests and 0.77 between SN and AGID tests, indicating very good to excellent agreement for HI and AGID tests with the SN test. Of 200 SN-positive samples, 19 samples with low SN titers (1:2-1:16) were further tested by Western immunoblotting, and all were confirmed as positive. Interpretation of the present results suggests that both HI and AGID tests can be used as alternatives to the SN test.  相似文献   

12.
In general, avian influenza (AI) vaccines protect chickens from morbidity and mortality and reduce, but do not completely prevent, replication of wild AI viruses in the respiratory and intestinal tracts of vaccinated chickens. Therefore, surveillance programs based on serological testing must be developed to differentiate vaccinated flocks infected with wild strains of AI virus from noninfected vaccinated flocks in order to evaluate the success of vaccination in a control program and allow continuation of national and international commerce of poultry and poultry products. In this study, chickens were immunized with a commercial recombinant fowlpox virus vaccine containing an H5 hemagglutinin gene from A/turkey/Ireland/83 (H5N8) avian influenza (AI) virus (rFP-H5) and evaluated for correlation of immunological response by hemagglutination inhibition (HI) or agar gel immunodiffusion (AGID) tests and determination of protection following challenge with a high pathogenicity AI (HPAI) virus. In two different trials, chickens immunized with the rFP-H5 vaccine did not develop AGID antibodies because the vaccine lacks AI nucleoprotein and matrix genes, but 0%-100% had HI antibodies, depending on the AI virus strain used in the HI test, the HI antigen inactivation procedure, and whether the birds had been preimmunized against fowlpox virus. The most consistent and highest HI titers were observed when using A/turkey/Ireland/83 (H5N8) HPAI virus strain as the beta-propiolactone (BPL)-inactivated HI test antigen, which matched the hemagglutinin gene insert in the rFP-H5 vaccine. In addition, higher HI titers were observed if ether or a combination of ether and BPL-inactivated virus was used in place of the BPL-inactivated virus. The rFP-H5 vaccinated chickens survived HPAI challenge and antibodies were detected by both AGID and HI tests. In conclusion, we demonstrated that the rFP-H5 vaccine allowed easy serological differentiation of infected from noninfected birds in vaccinated populations of chickens when using standard AGID and HI tests.  相似文献   

13.
Two diagnostic tests are approved for detecting antibody to equine infectious anemia virus: the agar-gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (ELISA). A total of 420 sera from National Veterinary Services Laboratories check sets were tested with the AGID and competitive ELISA. A 100% correlation was obtained. The AGID and competitive ELISA were further used to test difficult samples with low levels of equine infectious anemia antibody (weak positives). A third test (Western blot) was also used with these weak positive samples to resolve any discordant results.  相似文献   

14.
A study was conducted to compare the indirect enzyme-linked immunosorbent-assay (i-ELISA) test using antigen prepared by a simple technique using sodium dodecyl sulfate (SDS) treatment to the conventional agar gel immunodiffusion test (AGID). Ten specific-pathogen-free (SPF) sheep were inoculated with maedi-visna virus (MVV) and serum antibody titers compared over a period of 14 weeks. All the sheep seroconverted by the i-ELISA compared to 90% by the AGID. The i-ELISA detected antibody at a mean of 2.6 weeks prior to the AGID. In both tests, fluctuations were observed in the serum antibody response of two sheep. The i-ELISA had a specificity of at least 98.8% and an increased relative sensitivity of 15.5% compared to the AGID, based on the analysis of sera from experimental sheep with MVV free status and sera from sheep from various sources. Of the sera from a seronegative flock which had been monitored with the AGID after a "test and remove" eradication program, 10.2% were positive by the i-ELISA. It was concluded that the AGID test may not be adequate to monitor samples for an eradication scheme.  相似文献   

15.
Agar gel immunodiffusion (AGID) and counter-immunoelectrophoresis (CIEP), complement fixation (CF), radio-immunoassay (RIA), haemagglutination (HA) and haemagglutination inhibition (HI) tests were compared in their efficiency for the detection of bovine rotavirus antigens and antibodies. As a test for antigen using hyperimmune serum, CIEP was found to have advantages over AGID by being more rapid as well as approximately four times more sensitive regardless of whether the antigen was of faecal or tissue culture origin. The CF test was more sensitive than either of the immunodiffusion procedures studied for antigen detection, but was more tedious to perform and of limited use as some faecal samples exhibited anti-complementary activity. For measurement of rotavirus antibody the radio-immunoassay (RIA) was the most sensitive technique and the CIEP least sensitive. Using the RIA a limited survey of cattle demonstrated that approximately 75% of the animals tested possessed specific antibody to rotavirus.  相似文献   

16.
Avian influenza (AI) is a serious infectious disease caused by avian influenza virus (AIV) belonging to type A Orthomyxovirus. In the present study, we developed an indirect enzyme-linked immunosorbent assay (ELISA) employing E. coli-expressed full-length nucleoprotein (NP) of H9N2 avian influenza virus for the detection and quantification of antibodies against AIV nucleoprotein. The NP-ELISA was compared with the AI agar gel propagation (AGP) test, haemagglutination inhibition (HI) test, and IDEXX-FlockChek ELISA using 263 sera. The NP-ELISA was significantly more sensitive than the AGP and HI tests, and showed 96.2% agreement ratio with IDEXX-FlockChek ELISA. With results obtained using the NP-ELISA, an ELISA titre (ET) prediction equation, with which the ELISA titres of a flock or individual chickens can be determined, was derived from a positive/negative (P/N) ratio standard curve. The NP-ELISA enables an alternative rapid serological diagnosis and is suitable for influenza A antibody screening, especially in species that harbour several influenza subtypes.  相似文献   

17.
A maedi-visna virus CA-TM fusion protein ELISA (MVV ELISA) was evaluated for the detection of antibody in sheep infected with North American ovine lentivirus (OvLV). The results of the MVV ELISA were compared with other assays for OvLV antibody and with viral infection in an intensively studied group of 38 sheep with a high prevalence of OvLV infection and disease. The sensitivity, specificity, and concordance of assays for OvLV antibody (MVV ELISA, indirect ELISA, Western blot, and AGID), virus (virus isolation, PCR, antigen ELISA), and OvLV-induced disease in each animal were compared with OvLV infection status as defined by a positive result in two or more of the assays. Five sheep met the criteria for absence of OvLV infection. The sensitivity of the MVV ELISA in detecting OvLV infected sheep was 64%, whereas the sensitivity of the other three tests for antibody ranged from 85 to 94%. All the antibody assays were 100% specific in this group of animals. Of the assays for virus, the PCR test had the highest sensitivity and the best concordance with OvLV infection, but it also had the lowest specificity of any of the virus or antibody assays. Among the antibody tests, the concordance of the MVV ELISA compared most favorably with the AGID test for detecting OvLV-infected sheep. Analysis of serum samples from 28 lambs experimentally-infected with one of three North American strains of OvLV suggested that there were no significant strain differences detectable by antibody assay. Twenty virus-inoculated lambs were positive by both the MVV ELISA and the AGID test, five lambs were MVV ELISA negative and AGID test positive, and three lambs were MVV ELISA positive and AGID test negative. No pre-inoculation samples were positive by either assay. In a longitudinal study involving seven lambs, antibodies to OvLV were detected by AGID 3-5 weeks post-inoculation, but were not detected by MVV ELISA until 5-10 weeks post-inoculation. Among 128 naturally and experimentally-infected sheep that were seropositive in the AGID test, the overall sensitivity of the MVV ELISA was higher in the naturally infected sheep (84%) than in the experimentally infected sheep (69%). The data indicated that the MVV ELISA represents a less sensitive, but specific alternative for the detection of OvLV antibodies.  相似文献   

18.
采用RT-PCR技术从滨州分离株中扩增出传染性法氏囊病病毒(infectious bursal disease virus,IBDV)VP4基因,将VP4基因插入到pGEX-4T-1载体上构建pGEX-4T-1-VP4,诱导表达并用谷胱甘肽-S-转移酶(GST)亲和层析柱纯化得到纯化的重组VP4蛋白。以兔抗鸡IgG为胶体金标记物,以重组IBDV VP4蛋白和羊抗兔IgG为硝酸纤维素膜检测线和质控线的包被物,制备一种能检测IBDV VP4蛋白抗体的胶体金试纸条。结果表明,该试纸条检测IBDV强毒(IBDV BC6/85)免疫的血清检测线显红色,为阳性反应;检测IBDV弱毒(IBDV NB)免疫的血清、新城疫病毒(Newcastle disease virus,NDV)标准阳性血清、禽流感H5和H9标准阳性血清、传染性支气管炎标准阳性血清及0.85%生理盐水检测线不显红色,为阴性反应。该试纸条与建立的ELISA方法相比,敏感度低2个滴度;检测320份临床血清,试纸条与ELISA的符合率达99.38%。提示,该试纸条使用方便、操作简单,10 min内可以用肉眼判断结果,可为区分IBDV的强弱毒提供参考数据,具有较大的应用价值。  相似文献   

19.
In this study, antibody responses after equine influenza vaccination were investigated among 1,098 horses in Korea using the hemagglutination inhibition (HI) assay. The equine influenza viruses, A/equine/South Africa/4/03 (H3N8) and A/equine/Wildeshausen/1/08 (H3N8), were used as antigens in the HI assay. The mean seropositive rates were 91.7% (geometric mean antibody levels (GMT), 56.8) and 93.6% (GMT, 105.2) for A/equine/South Africa/4/03 and A/equine/Wildeshausen/1/08, respectively. Yearlings and two-year-olds in training exhibited lower positive rates (68.1% (GMT, 14) and 61.7% (GMT, 11.9), respectively, with different antigens) than average. Horses two years old or younger may require more attention in vaccination against equine influenza according to the vaccination regime, because they could be a target of the equine influenza virus.  相似文献   

20.
Recently a commercial enzyme-linked immunosorbent assay (ELISA) kit for detecting antibody against H1N1 swine influenza virus (SIV) has been made available to diagnosticians and veterinary practitioners. Because the hemagglutination inhibition (HI) test has been considered the standard test for SIV serology, diagnostic performance of the new ELISA was evaluated using positive (n = 60) and negative (n = 188) serum samples from young pigs with known status of SIV infection and compared with that of the HI test. Both ELISA and HI test identified all negative animals correctly. None of the serum samples (n = 64) from pigs inoculated with H3N2 SIV was positive by ELISA for SIV antibody. The H1N1 SIV antibody detectable by ELISA appears to develop more slowly in comparison with antibody detectable by HI test. Although antibody was detected by HI test in all inoculated animals (n = 20) by day 7 postinoculation (PI), antibody was detected by ELISA in 0%, 75%, and 100% of the inoculated animals on days 7, 14, and 28 PI, respectively. Discrepancy in test results between the 2 serologic tests appeared to be because of differences in antibody isotypes detected by each test. Enzyme-linked immunosorbent assay mainly detected IgG antibody, whereas the HI test detects IgM antibody very efficiently as well as IgG antibody. Collectively, the commercial ELISA is highly specific for antibody to H1N1 SIV but may not identify positive animals at the early stage of infection as effectively as the HI test, particularly when SIV is introduced to a na?ve swine population.  相似文献   

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