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利用分隔网片将桁拖网渔具网囊分为上下2层(虾囊和鱼囊)以实现虾类和鱼类渔获分离,对于改善桁拖网渔具作业性能、提高目标种类渔获质量以及实现选择性捕捞具有重要的意义.为分析不同网日尺寸(60mm、75mm和90mm)分隔网片对主要渔获种类的分隔效率,在吕泗渔场开展了2个航次的海上生产实验.利用分隔装置性能分析模型,对哈氏仿对虾(Parapenaeopsis hardwickii)和葛氏长臂虾(Palaemon gravier)的渔获数据进行拟合.结果表明,不同虾类接触分隔网片的概率没有显著性差异;虾类个体50%接触概率体长约为40mm,表明大多数进入网具的虾类都能接触分隔网片.随着分隔网片网目尺寸增大,50%选择体长也逐渐增大.因此,分隔网片的分隔效率随之提高.鱼囊和虾囊的网目尺寸相同,但结构有所差异.比较在不同网囊尺寸选择性假设条件下渔获数据的拟合结果后发现,鱼囊和虾囊具有相同选择性的假设更为合理;通过比较发现,分隔网片对虾类的尺寸选择性与相同网目尺寸的网囊选择性具有较大的差异.本研究还对产生这一差异的原因进行了探讨. 相似文献
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广东大鹏湾虾拖网网囊网目选择性研究 总被引：2，自引：4，他引：2
虾拖网渔业是南海海域重要的作业方式之一,但尚未有关该渔业网目尺寸选择性的报道。为评价虾拖网渔具的渔获性能、合理利用虾类资源,在广东大鹏湾进行了网目选择性试验。试验采用双支架虾拖网作为试验网具,并使用套网法估算了网目内径为35、40和45 mm的网囊对主捕虾类的选择性。结果显示,虾类渔获中刀额新对虾、须赤虾、周氏新对虾和日本囊对虾渔获数量较多,比较以往资料,刀额新对虾和周氏新对虾优势体长、平均体长均有所下降,其它2种虾类平均体长未见明显变化;选择性分析结果显示,主捕虾类的L50随网目尺寸线性增大。根据试验结果并结合历史资料,笔者认为现阶段35 mm的网囊网目尺寸对于保护上述4种虾类资源是比较合适的。 相似文献
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传统捕虾桁拖网渔具网目小、渔获对象繁杂、选择性能差。使用网片式分隔装置、实现渔具的选择性捕捞是缓解这一问题的有效手段之一。2005年秋汛期间,在吕四渔场桁拖网渔业中开展了网目尺寸分别为60、75和90 mm分隔网片的海上生产试验,本文通过蟹类渔获为研究对象,分析分隔网片对其的分隔效果。试验结果显示,使用以个体接触分隔网片概率和网片对个体的留存率为基础的分隔效率模型,对蟹类甲宽分布数据的拟合发现,随着网目尺寸的增大,分隔网片对三疣梭子蟹(Portunus trituberculatus)、细点圆趾蟹(Ovalipes punctatus)的50%留存甲宽和留存率范围均有所增大;而上述2种蟹类个体接触不同网目尺寸分隔网片的概率则没有明显的一致性变化趋势。试验结果表明,蟹类在到达桁拖网网身部时具有一定的游泳能力且具有一定的游泳高度,也反映出使用向下倾斜的分隔网片在结构和装配上是比较合理的。 相似文献
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桁拖网作业中鱼虾分隔的初步试验研究 总被引：12，自引：3，他引：9
1996年末,我们在浙江渔场进行了桁拖网作业的鱼虾分隔试验,如同我们过去对这类渔具所作的放大网目以释放鱼、虾幼体;伸长吊纲,提高网口以增加对三疣梭子蟹的捕捞产量(季星辉等,1984)一样,本次试验也是这类网具选择性研究的内容之一,目标是改善桁拖网的选择性。在提高这种作业经济效益的同时,充分兼顾保护渔业资源和海洋生态环境的社会效益。 试验工作是在现有多囊捕虾桁拖网上,选择其中一个囊网,用一片目大为100mm的网片进行分隔,使鱼进入原囊网下方增设的盛鱼囊袋,而虾因其遇惊会弹跳的习性,穿过分隔网片进入原囊网。实践证明,分隔的效果是明显的,虾的分隔率达到55～68％,鱼的分隔率达到95％。现有的试验是初步的,我们将创造条件继续下去。 相似文献
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在虾拖网渔业的混合渔获中鱼的平均分布率高达81％，不仅鱼类资源有巨大浪费，而且对鱼类资源的繁殖也产生严重影响。为了减少幼鱼副渔获，经常采用网囊的网目选择性。但是，早期的研究证明，许多变量影响拖网和其他移动性渔具中鱼类的尺寸选择。要克服的最大问题之一就是网囊渔获增加后有效网目张开减小。 相似文献
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大网目拖网是我国海洋渔业捕捞带鱼的重要渔具。为提高拖网网囊选择性, 本研究在南海区采用套网法对双船大网目拖网 4 组网目尺寸(30、35、40 和 45 mm)的网囊进行实验, 在双重拔靴法的架构下, 拟合选择性参数、比较选择性的差异、估算捕捞方式指标。结果表明, 随着网目尺寸的增大, 网囊对带鱼的 50%选择体长增大, 对带鱼幼鱼个体的选择率显著下降, 带鱼幼鱼的渔获比例也呈下降趋势。结合带鱼最小可捕规格(23.0 cm)管理规定, 结果还表明网目尺寸为 40 mm 的网囊选择性较差, 其对带鱼的抛弃率大于 93%。为了进一步提高带鱼拖网渔业的资源养护水平, 还需要开展更多的选择性实验。 相似文献
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多囊桁拖网选择性研究中对照网囊网目尺寸的选择 总被引：2，自引：1，他引：1
对比作业法是拖网渔具选择性研究中重要的试验方法之一,而对比试验中对照网囊网目的选择至关重要。研究利用多囊桁拖网不同网目尺寸(20、30、35和40mm)网囊捕获的哈氏仿对虾、小黄鱼和棘头梅童鱼渔获体长分布数据,应用几何相似原理,分析过滤性渔具的选择性,并按不同假设条件下(假设1:符合几何相似原理对渔获具有一定的选择性;假设2:对渔获种类没有选择性),探讨如何合理选择对照网囊网目尺寸。结果表明,对照网网囊网目尺寸为20mm,在上述2种假设下,对选择性模型的拟合、简化及对其它网囊的选择性参数没有显著的影响;在考虑了桁拖网渔具各网囊具有相同相对作业强度,以及桁拖网渔具与普通拖网渔具选择性分析方法上的差异等因素后,认为在此项研究中,将小于20mm网目尺寸的网囊视为没有选择性的对照网囊是可行的。 相似文献
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<正> 目前我国渔民捕虾用的渔具有拖网、流刺网、张网和敷网,其中以拖网的捕捞效率为最高。但是,在虾拖网渔业中,鱼虾混获却成为我国虾拖网渔业者长期以来迫切期望解决的一个重大难题。一般来说,虾拖网的网目尺寸比鱼拖网的网目尺寸小得多,所以这种虾拖网在捕虾作业过程中不可避免要混 相似文献
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Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean 
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下载免费PDF全文 The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna. 相似文献
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Liping Li Rui Wang Wanwen Liang Xi Gan Ting Huang Yan Huang Jian Li Yunliang Shi Honglin Luo Ming Chen 《广西水产科技》2014,(1)
Previously, we reported 10 PEGE types of 85 tilapia Streptococcus agalactiae(GBS), which shifted from Streptococcus iniae in China, by using PEGE method. Presently, larger and more representative tilapia GBS were isolated, for the ?rst time in China, to characterize their serotypes and genetic diversities more precisely than had done before. 168 GBS strains were distributed in ?ve provinces of China, in which Guangdong, Guangxi and Hainan were the major ones, holding36.9%(62/168), 37.5%(63/168) and 19.6%(33/168), respectively. Serotypes, Ia, Ib and III, were observed in these strains and the most predominant one was Ia(95.2%), which mainly distributed in Guangdong, Guangxi and Hainan. Ia initially occurred in 2009, it shoot up to 32.1% in 2010,but decreased to 16.1% in 2011 before went up to 45.2% in 2012. Ib sporadically occurred during2007–2011, III onlyoccurred in 2012. 14 different PFGE types, including 4 new types(N, O,P and Q), were observed, in which B, D, F and G were the predominant types, holding 83.9%(141/168) of the total GBS strains. Ia corresponded to 11 PFGE types(A–H, N–P), in which type D predominated(51%). Ib represented 3 genotypes(I, J and Q) and III harbored only 2genotypes(N and F). Type N and Fsynchronously presented in Ia and III. In summary, the genetic diversity of tilapia GBS varied by serotypes and changed with geographical locations and years.Although Iastillpredominated, new rareserotypeIII alreadyoccurred in China. 相似文献
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In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg. 相似文献
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Iciar Martinez Amaya Velasco Ricardo Pérez-Martín Eskil Forås Michiaki Yamashita Carmen G. Sotelo 《Journal Of Aquatic Food Product Technology》2013,22(4):289-297
The purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus. 相似文献
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Two gonadotropins (GtH; Qa and Qb) were purified by gel filtration and ion exchange chromatography from the pituitaries of Indian walking catfish (Clarias batrachus). The presence of GtH during purification was assessed by in vitro oocyte maturation and in vivo steroidogenic activity, and their identities were determined by elution profiles, molecular weight, biological activities and yield. The molecular weights of Qa and Qb were 37 and 42 kDa, respectively, and composed of distinct subunits (Qa: 20 and 14 kDa and Qb: 26 and 18 kDa). Polyclonal antibodies raised against Qa immunostained Qa, Qb and pituitary GtH cells. A competitive Qa‐ELISA was developed whose sensitivity was 6.25 ng mL?1 (1.25 ng well?1) with intra‐ (3.5%) and inter‐ (12.4%) assay coefficients of variation. Displacement curves parallel to the standard were obtained with plasma and pituitary extracts of catfish, Qb and carp GtHII. The assay was validated by measuring the plasma Qa levels after LHRH treatment and in relation to ovarian growth in the female catfish during different reproductive phases. Based on the results, Qa and Qb corresponded to fish LH and FSH respectively. The findings will increase the knowledge of the mechanisms controlling fish reproduction and identification of sensitive phases in fish in captivity for hormonal manipulation. 相似文献
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Controlled infection of Poecilia reticulata Peters (guppy) with Tetrahymena by immersion and intraperitoneal injection 下载免费PDF全文
下载免费PDF全文 G Sharon M Pimenta‐Leibowitz M C L Vilchis N Isakov D Zilberg 《Journal of fish diseases》2015,38(1):67-74
Tetrahymena is a protozoan parasite, which infects guppy, Poecilia reticulata Peters, and causes substantial economical losses in commercial farms worldwide. Studies of guppy infected by Tetrahymena require standardized infection protocols. The LD50 for Tetrahymena infection of guppies by intraperitoneal (IP) injection was calibrated, and the level obtained was 946 parasites per fish. Guppy infection with Tetrahymena by immersion, imitating the natural route of infection via the integument, was studied under normal or stress conditions. Exposure to cold and netting (CNI) and to cold only (CI) followed by immersion exposure to 10 000 Tetrahymena per mL resulted in 22.5% and 19.2% mortality, respectively, as compared to 14.2% and 10% in groups that were netted only (NI) or non‐stressed (I). Histopathology revealed that immersion infection resulted in a systemic infection. Lysozyme levels, measured 3 weeks after infection, were significantly higher in the CNI group (288 μg per mg protein) compared with CI‐, NI‐ and I‐treated groups (94.5, 64 and 62.3 μg mg?1, respectively). There was no evident parasite immobilization activity in body homogenates, suggesting no development of acquired immunity. Re‐infection by IP injection revealed no increase in protection in any of the treatment groups, mortality range of 56.3–75%, higher than in the non‐exposed control (40.6% mortality). 相似文献
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Qin-Cheng Huang Di‐Gen Qin Bei‐Ping Tan Tao Du Yuan-Zhi Yang Qi-hui Yang Shu‐Yan Chi Xiao-hui Dong 《Aquaculture Research》2020,51(2):816-827
A 5 × 3 factorial growth trial was conducted to evaluate optimal dietary protein and lipid levels (dietary protein level, DP; dietary lipid level, DL) for juvenile Sillago sihama (S. sihama) (2.0 ± 0.02 g, initial weight). Fish were fed 15 diets containing 5 DPs (350, 400, 450, 500 and 550 g/kg) and 3 DLs (60, 90 and 120 g/kg) for 8 weeks. The interaction between proteins and lipids significantly influenced the feed conversion ratio, condition factor, body composition, antioxidant indices and lipase activity (p < .05). DP 450 g/kg showed the highest average final body weight. DPs 500 and 550 g/kg significantly decreased the protein efficiency ratio (p < .05). DL 120 g/kg showed the highest percentage weight gain. The low feed conversion ratio was found in diets P45L12, P55L9 and P55L12. Diet P45L12 showed high superoxide dismutase activities. DP 450 g/kg showed the lowest average malondialdehyde content. Lipase activity was increased by increasing DP (p < .05) with a fall at DP 550 g/kg. Under the present experimental conditions, the optimal DP for S. sihama was 450 g/kg under the DL 120 g/kg. 相似文献
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William King V David L. Berlinsky Craig V. Sullivan 《Fish physiology and biochemistry》1995,14(6):489-500
Plasma estradiol-17 (E2), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E2 and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E2 and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass. 相似文献