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山东省猪群TTV感染情况及分子流行病学分析 总被引：2，自引：1，他引：1
为了确定Torque teno virus(TTV)在山东省猪群中是否存在及其感染情况,本研究利用套式PCR方法检测了来自山东省不同地区10个猪场的230份血液样品和组织样品。结果表明猪群中TTV1和TTV2感染总阳性率分别为45.2%和85.7%。两者的混合感染率为39.3%。用套式PCR引物扩增出TTV1和TTV2的部分片段,并将扩增出的部分基因与已报道的TTV1和TTV2病毒序列进行比较分析。分析结果显示TTV可分为TTV1和TTV2两大分支,其TTV1和TTV2与参考株相比同源性分别为88.0%～92.8%和91.9%～94.1%。本研究证实在山东省猪群中存在TTV感染,由于TTV可以感染人,其在公共卫生学方面的意义应当引起我们的重视。 相似文献
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我国猪群中TTV的鉴定及其分子流行病学分析 总被引：10，自引：2，他引：8
为了确定Torque teno virus(TTV)在我国猪群中是否存在及其感染情况,本研究利用套式PCR方法首次检测了来自广东、福建和江西等7个省份的258份血液样品和组织样品.结果表明猪群中TTV1和TTV2感染总阳性卒分别为37.6%和82.6%.两者的混合感染率为38.4%.挑选部分阳性样品用套式PCR引物扩增出TTV1和TTV2的部分非编码区(UTR)片段,并将扩增出的部分UTR基因与已报道的TTV1和TTV2病毒UTR序列进行比较分析,同时我们选择了8份阳性样品进行了TTV1和TTV2的全长基因的扩增和克隆.分析结果显示TTV可分为TTV1和TTV2两大分支,其中我们分离获得的TTV1和TTV2之间的UTR核苷酸同源性分别为80.8%～98.5%和94.2%～100%,而与参考株相比同源性分别为82.7%～100%和95.5%～99.1%.本研究首次证实在我国猪群中存在TTV感染,提示我们对猪群感染的TTV是一个不容忽视的新病原. 相似文献
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为了解猪细环病毒(TTV)在无明显临床症状的猪群和不同发病猪群中的感染情况,本实验于2011年～2012年期间共采集江苏、安徽、天津、湖北、上海、浙江6个省份的无明显临床症状的母猪血清共488份,发病猪群样品352份,包括发生PMWS的猪血清及组织样品203份,发生PRRSV感染的猪群样品105份,发生流产的初产母猪血清23份,以及流产胎儿组织样品21份.对无明显临床症状的猪群进行TTV1和TTV2的病原学检测,对发病猪群样品进行TTV和PCV2、PPV4、PRRSV共感染检测,并对40条TTV1和32条TTV2的UTR部分基因分群区域进行测序和进化分析.结果显示,在488份无明显临床症状的母猪和种公猪中,猪源TTV1和TTV2感染率较高,分别为41％和67％,二者混合感染率为28％.PMWS发病猪样品中PCV2阳性率高达75％,PCV2和TTV2的混合感染高达61％.在PRRSV为阳性的病料中,TTV2的阳性率高达86％.在初产母猪和流产胎儿中,TTV1和TTV2的感染率,以及与不同病毒的混合感染率均高于健康猪群的感染率.对基因序列的进化分析表明,TTV1主要可以分为8个基因群,TTV2可以大致分为4个基因亚群.本研究为研究猪源TTV的潜在致病力以及分子进化提供了进一步的资料. 相似文献
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根据GenBank_h发表的猪圆环病毒2型（Porcinecircovirustype2,PCV2）基因组序列和TTV（Torquetenovirus）1、2型的UTR序列设计合成引物,建立了分别用于检测PCV2和TTV1、TTV2的PCR及巢式PCR方法。应用建立的PCR方法对送检的广东、福建和江西等7个省份258份血液和组织样品进行了PCV2、TTV1和TTV2的检测,确定猪群中PCV2与TTV1和／或TTV2混合感染情况。结果表明,94份样品表现为PCV2和TTV1的混合感染,占样品总数的36．4％;193份样品表现为PCV2和TTV2的混合感染,占74．8％;另外,还有一些样品为三重感染,占34．5％。由此可以看出,猪群中PCV2和／或TTV1和／或TTV2的混合感染很普遍。 相似文献
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猪输血传播病毒(TTV)与猪圆环病毒混合感染的检测及TTV遗传变异分析 总被引：1，自引：0，他引：1
为了解我国猪群中猪输血传播病毒(TTV)与猪圆环病毒(PCV)混合感染情况,本研究采用PCR方法对采自14个省280份病料样品进行检测,结果显示,TTV1和TTV2阳性检出率分别为51.8%和28.2%,TTV1与TTV2混合感染率为18.2%;PCV1和PCV2阳性率分别为41.1%和37.5%,PCV1与PCV2混合感染率为19.6%;PCV2阳性样品中TTV1和TTV2混合感染率达75.0%。此外,对2株TTV1基因组进行测序,与GenBank中登录的6个TTV1序列比对,相似性结果为67.3%～95.1%;对4株TTV2基因组测序,与GenBank登录的4条TTV2序列比对,相似性为84.7%～90.4%;将TTV1与TTV2进行序列比对,相似性仅为44.0%。TTV1和TTV2基因高变区分别位于520 nt～2 594 nt和720 nt～2 170 nt;TTV1基因保守区位于1 nt～520 nt和2 595 nt～2 800 nt;而TTV2基因保守区位于1 nt～719 nt和2 171 nt～2 800 nt。以上结果表明,我国猪群中存在TTV与PCV2的混合感染,并且两种病毒混合感染引起... 相似文献
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2009年我国部分猪群输血传播病毒感染情况调查 总被引：1，自引：0，他引：1
为调查输血传播病毒(TTV)在我国猪群中的感染状况,本研究对2009年采自29个省市的1990份猪血清样品进行了TTV1、TTV2的双重PCR方法检测,并对结果进行了统计分析.结果表明,我国猪群中TTV总的感染率为63.37％(1261/1990),其中TTV1感染率为55.88％(1112/1990),TTV2为32.91％ (655/1990),TTV1和TTV2双重感染率为25.43％(506/1990).进而对样品的地区性分布特征、样品来源等影响因素进行分析,表明我国猪群中TTV感染率以东北地区最高,西北地区最低.样品来源不是影响感染率的关键因素. 相似文献
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为了解细环病毒(Torque teno virus,TTV)在广西猪群中的感染情况,本研究运用Nest-PCR方法,对2009—2011年采自广西140个规模猪场的156份血液、流产胎儿及肺脏、脾脏、肾脏、肝脏、淋巴结等组织样品进行检测,并对阳性样品的非编码区(UTR)进行克隆测序及遗传进化分析;同时对部分样品进行猪圆环病毒2型(PCV2)、猪繁殖与呼吸综合征病毒(PRRSV)、典型猪瘟病毒(CSFV)、伪狂犬病病毒(PRV)检测及细菌的分离鉴定。结果发现,广西猪群中TTV总感染率达到93.6%,TTV2的感染率(76.9%)不仅明显高于TTV1(16.7%),且毒株间遗传变异较大。TTV多与PCV2和PRRSV混合感染,且以与PRRSV混合感染率更高(64.29%)。猪群中存在2重、3重,甚至4重TTV与其他病毒的混合感染。临床上TTV与细菌的混合感染(或细菌继发感染)以链球菌和副猪嗜血杆菌多见。本研究证实在广西猪群中存在TTV感染,且存在普遍的TTV与PRRSV、PCV2和CSFV混合感染。 相似文献
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为建立输血性传播病毒(TTV)基因1型(TTV1)和TTV基因2型(TTV2)的检测方法,本研究根据TTV1与TTV2基因的非编码区域序列差异,设计了2对特异引物,建立了鉴别TTV1和TTV2的SYBR-GreenⅠ实时荧光PCR方法。分别进行了特异性、敏感性及重复性检验,结果表明:重组质粒建立的标准曲线相关系数为99%;可以同时鉴别检测TTV1和TTV2,检测PCV2和PRRSV阳性样品均呈阴性,表明该方法具有良好的特异性;最低检出量为10 copies/μL;重复试验结果显示,TTV1组内和组间变异系数分别为0.14%和0.74%;TTV2组内和组间变异系数均为0.13%;检测现地采集的189份样品,TTV1阳性率32.3%,TTV2阳性率6.3%,TTV1和TTV2混合感染的阳性率28.6%。TTV检测方法的建立为猪群TTV病的流行病学调查提供了有效的手段。 相似文献
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为了解猪细环病毒Ⅰ型（TTV1）的流行情况,对莱西、城阳、胶州、莱阳四家规模化养猪场的59份血清进行了TTV1分子流行病学调查,从血清中提取病毒DNA,然后通过TTV1引物进行PCR检测样品的感染情况。检测结果表明,所检测的4家养猪场均检测到TTV1感染。其中,莱西某猪场的感染率最高,为55.2%;莱阳、城阳、胶州猪场的感染率分别为30%、10%、20%。目前TTV1不能造成直接的致病作用,但是在我国很容易与其他病原发生混合感染,导致协同致病,因此了解TTV1在猪场中的存在情况具有重要意义。 相似文献
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Osamu Taira Hirohito Ogawa Aki Nagao Kotaro Tuchiya Tetsuo Nunoya Susumu Ueda 《Veterinary microbiology》2009,139(3-4):347-350
Torque teno virus (TTV) was first isolated from a human hepatitis patient in 1997. TTV was also identified in several animals, including pigs, cattle, sheep, cats and dogs. In this study, we analysed the prevalence of swine TTV genogroups 1 (TTV1) and 2 (TTV2) in Japanese swine populations with suspected post-weaning multisystemic wasting syndrome and porcine respiratory disease by using a nested polymerase chain reaction method. Of 153 serum samples from 16 different herds in Japan, TTV1 was detected in 46 samples (30%), TTV2 in 47 samples (31%) and both in 15 samples (10%). There was no significant difference in the detection rate among geographical regions. The overall prevalence rate of TTV genogroups was significantly lower in ≤30-day-old pigs (11%) compared to that in older age groups (54–82%). These results suggest that swine TTV may be widespread in post-weaning pigs and could play aetiological roles in pig diseases in Japan. This is the first report on the prevalence of swine TTV in Japan. 相似文献
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Torque teno viruses (TTVs), of the genus Anellovirus, are single-stranded circular DNA viruses that infect many vertebrate species. Although viruses of this type have quite a stable genome, they exhibit low nucleotide homology. Torque teno virus infection has not been consistently linked to specific diseases, although there is epidemiological evidence of an association with disease in humans. The recent recognition of naturally occurring TTV infection in swine and its epidemiological resemblance to human TTV raises the possibility of using the pig as a model to study human TTV infection. Such an approach will require the development of novel investigative tools to study the epidemiology, transmission, immune responses and potential pathogenesis of TTV infection. The present review summarises research on animal TTV infection, focussing in particular on TTV infection in the pig, and considers how a porcine experimental infection model might assist in the study of human TTV infection. 相似文献
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Molecular characterization of porcine TT virus, an orphan virus, in pigs from six different countries 总被引：2，自引：0，他引：2
Human TT virus (TTV), originally isolated from a patient with post-transfusion hepatitis in 1997, is ubiquitous and non-pathogenic. Viruses related to human TTV have since been identified in non-human primates, bovine, ovine, porcine, feline, and canine. The objective of this study was to genetically characterize porcine TTV from pigs in different geographic regions. PCR primers based on the non-coding region of the only available porcine TTV isolate were designed to amplify porcine TTV DNA from sera of pigs in six different countries. Porcine TTV DNA was detected in 66.2% (102/154) of the swine sera. The percentages of positive pigs varied greatly from country to country and even within the same country: 33% in Iowa, USA; 40% in Thailand; 46% in Ontario, Canada; 80% in China; 85% in Korea; 90% in Spain; 100% in Quebec and Saskatchewan, Canada. A total of 40 porcine TTV isolates (five from each geographic region) were sequenced for a 218 bp fragment within the non-coding region. Sequence analyses revealed that porcine TTV isolates from different geographic regions shared 86-100% nucleotide sequence identity to each other. The prototype Japanese isolate of porcine TTV, Sd-TTV31, shared 90-97% nucleotide sequence identity with porcine TTV isolates reported in this study. Phylogenetic analysis showed that the clustering of the porcine TTV isolates is not associated with geographic origins. Although porcine TTV is not known to be associated with any swine disease, co-infection of pigs with TTV and other known swine pathogens may result in enhanced disease. There are also concerns for risk of potential human infection during xenotransplantation. 相似文献
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Martelli F Caprioli A Di Bartolo I Cibin V Pezzotti G Ruggeri FM Ostanello F 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2006,53(5):234-238
Anellovirus is a recently created, floating genus of viruses. Torque teno virus (TTV), the type species in the genus, was first discovered in a human patient with a post-transfusion hepatitis of unknown aetiology. Recently, TTV genetically related to but distinct from those discovered in humans have also been found in animals, including pigs. The aims of this study were to estimate the prevalence of swine TTV in Italian pig herds and some risk factors possibly associated with this infection. Serum samples from 179 healthy pigs from 10 farms located in north-central Italy were tested by polymerase chain reaction for the presence of swine TTV DNA. Viral DNA was found in the sera of 43 pigs (24.0%), coming from eight of the 10 farms examined. Prevalence was significantly higher in finishing herds (40.1%) than in farrow-to-finish herds (11.0%) and did not depend on the size of the herd. Within the finishing herds the prevalence was significantly higher in weaners (57.4%) than in fatteners (22.9%), but this difference was not observed in farrow-to-finish herds. No relationship was observed between the prevalence of swine TTV and the implementation of some general hygiene practices and biosecurity procedures within the herds. 相似文献
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细环病毒（TTV）目前在全世界的猪群中广泛存在。为了调查其在广东省猪群流行情况,我们运用PCR方法对广东省不同地方猪场和散养户送检的445份血清进行了检测。结果表明：TTV总的阳性率为68.1%（303/445）,其中TTV1阳性率为43.1%（192/445）,TTV2阳性率为24.9%（111/445）。TTV1的阳性率高于TTV2的阳性率,且猪场送检样品阳性率高于散养户。TTV1和TTV2存在混合感染,猪场样品混合阳性率为23.1%（88/381）,散养户样品混合阳性率为1.6%（1/64）。几份阳性样品测序和进化分析显示,它们分别属于TTV1和TTV2。调查结果表明TTV在广东猪群中已经广泛存在。 相似文献
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Lang C Söllner H Barz A Ladinig A Langhoff R Weissenböck H Kekarainen T Segalés J Ritzmann M 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(3-4):142-147
In the present retrospective study, serum samples from a total of 253 pigs of different age groups from 83 farms collected in the year 2008 were included.The sera were analysed for the presence of torque teno virus (TTV) genogroups 1 and 2. Furthermore, the correlation between TTV and PCV-2 was investigated. 62.1% of the pigs were positive for TTV1 and/or TTV2 by PCR. 21.7% of the samples were positive for TTV1 and TTV2, 16.2% only for TTV1 and 23.3% only for TTV2. In 80.7% of the investigated farms at least one animal was TTV positive. In the growing and fattening period significantly more pigs were positive for TTV than in other age classes. A positive association was found between TTV1 and IgM against PCV-2, between TTV2 and PCV-2 results by in situ hybridisation (ISH) as well as TTV1 and TTV2 and PCV-2 results by qPCR. A significant correlation could also be seen between the diagnosis,porcine respiratory disease complex (PRDC)" and the detection of TTV, whereas there was none to the diagnosis "porcine dermatitis and nephropathy syndrome (PDNS)". In the present study, TTV was detected in Austria for the first time. 相似文献
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S Noppornpanth T Chinchai P Ratanakorn Y Poovorawan 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(6):663-666
TT virus (TTV) is not only an infectious agent of worldwide distribution but has also been demonstrated in various non-human primates in addition to humans. In the present study, we subjected the sera of 67 gibbons to PCR and nucleotide sequencing, with subsequent phylogenetic analysis to determine the nature of the relationship between TTV found in humans and non-human primates. We discovered the virus in 9/67 (13.4%) of the gibbon sera and subjected 6 of those to direct sequencing. The phylogenetic tree constructed encompassed all TTV species known to date, revealing a close proximity between the gibbon virus and those detected in Thai individuals, whereas the chimpanzee strains were phylogenetically more remote. 相似文献