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猪多杀性巴氏杆菌的分离鉴定和药敏试验 总被引：1，自引：0，他引：1
为了对河南省猪高热综合征发生期间多杀性巴氏杆菌引起的感染有全面了解,从2006-2010年河南省发生猪高热综合征的245家规模化猪场568份病料中分离革兰氏阴性小球杆菌,通过培养特性试验、生化试验和PCR试验分离鉴定到35株。对分离菌进行毒力和药敏试验,结果显示所分离的猪多杀性巴氏杆菌对小鼠均有毒力,对头孢噻呋、头孢噻肟、环丙沙星、氟苯尼考敏感。 相似文献
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从病死母猪肺脏中分离到一株革兰氏阴性小杆菌,用生理生化鉴定、药敏试验、致病性试验和PCR鉴定方法对分离菌株进行鉴定,并用多杀性巴氏杆菌荚膜分型引物对分离株的荚膜血清型进行鉴定。结果表明:本菌为猪多杀性巴氏杆菌,对多种抗生素高度敏感,对小白鼠有强致病性;PCR扩增16SrDNA基因获得1415bp片段,分离株的16SrDNA核苷酸序列与多杀性巴氏杆菌(AY078999)的同源性为99%,因此该分离菌株被鉴定为致病性巴氏杆菌,命名为YN20110122株;本菌分离株为荚膜A型血清型多杀性巴氏杆菌。 相似文献
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本试验选择了石河子地区部分规模化猪场为研究对象,采集疑似由呼吸道引起死亡的仔猪病变组织共36份,进行该细菌的分离鉴定,同时采用纸片扩散法（K-B法）对分离株的耐药性进行了检测。经过对组织病料进行分离鉴定,结果共分离出猪多杀性巴氏杆菌共12株,该菌的检出率33.3%,经过对其中4株菌分别为XNC001、XNC002、XNC003、XNC004进行了小鼠接种试验,结果4株菌致死率分别为25%、100%、100%、75%,表明该地区各个规模化猪场流行的猪多杀性巴氏杆菌致病有差异显著,同时对该地区分离的12株猪巴氏杆菌药敏试验可知,该菌对先锋Ⅵ,环丙沙星,氯霉素敏感性较强,其敏感率分别为83.3%、75%、75%,而该细菌均对其他几种抗生素的耐药性较强,该细菌对四环素的耐药率达到了66.7%。 相似文献
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为了确定和分析新疆某羊场患呼吸道疾病病例的细菌性病原，采集病死羊的肺脏、胸腔积液等样品进行细菌分离，通过生化试验、16S rRNA基因测序、特异性引物PCR鉴定、药物敏感性试验、毒力基因测定、对小鼠半数致死量(LD50)测定等方法对分离株进行生物学特性研究。结果显示，从病羊胸腔积液中分离鉴定出一株荚膜血清D型多杀性巴氏杆菌，将其命名为Pmh7。Pmh7对链霉素、头孢呋辛等10种抗菌药物敏感，对万古霉素、杆菌肽等5种抗菌药物耐药；携带6个毒力基因(fimA、nanB、ompH、sodC、Oma87、sodA),对小鼠的LD50为2.3×10~6 CFU。结果表明，引发该羊场呼吸系统疾病的病原为荚膜D型多杀性巴氏杆菌，有较强的致病性。本研究结果可为防治羊巴氏杆菌病提供参考，为巴氏杆菌病的流行病学监测奠定基础。 相似文献
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为了确定西南民族大学动物医学实验室分离的鸭源、猪源、牛源和山羊源多杀性巴氏杆菌(Pm),即Q1、Z1、N1、Y1株的荚膜血清型及其致病性,试验采用PCR方法对分离的这4株不同动物源多杀性巴氏杆菌的种属和荚膜血清型进行鉴定,对目的基因测序分析,并用Balb/c小鼠对分离菌的致病性进行研究。结果表明:4株菌均为多杀性巴氏杆菌;鸭源Q1株为荚膜血清A型、猪源Z1株和牛源N1株为荚膜血清B型、山羊源Y1株为荚膜血清D型;对目的基因测序后与GenBank上已公布的相应荚膜血清型比对,同源性为98%～100%;不同动物源多杀性巴氏杆菌对Balb/c小鼠的致病性研究显示,猪源荚膜血清B型多杀性巴氏杆菌对Balb/c小鼠有较强的致病力。 相似文献
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猪多杀性巴氏杆菌的分离鉴定及生物学特性研究 总被引：17，自引：2，他引：17
用PCR方法配合生化鉴定，从有肺炎症状猪的肺脏及进行性萎缩性鼻炎(Progressive atrophic rhinitis，PAR)症状猪的鼻拭子中分离出66株多杀性巴氏杆菌(Pasteurella multocida，Pm)。然后做了药敏试验，并用PCR方法对这66株Pm进行分型及毒素基因的检测，用豚鼠皮肤坏死试验及小鼠致死试验对产毒素多杀性巴氏杆菌(Toxigenie Pasteurella multocida，T^ Pm)进一步鉴定。结果显示PCR鉴定与生化鉴定Pm结果完全一致；PCR分型表明有46株为D型Pm，18株为A型：Pm，1株为B型Pm，1株无法定型；有8株用PcR检测为T^ Pm；豚鼠皮肤坏死试验及小鼠致死试验对这8株T^ Pm的进一步鉴定也表明均为产毒素菌株。所鉴定的8株T^ Pm都为D型，都分离于有严重PAR症状的猪。 相似文献
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为探明2022年2月玉溪市江川区某规模猪场大批保育猪发病死亡原因，对采集病料进行了细菌分离，从肺门淋巴结分离得到一株菌落表面光滑、湿润、半透明圆形菌株。分离菌株经生化试剂条鉴定为多杀性巴氏杆菌，分离菌株的16S rRNA序列与NCBI GenBank中公布的多杀性巴氏杆菌PM8-6株的同一性达到99%以上，将分离菌株命名为PMJC-1株。荚膜分型结果显示该菌株为荚膜A型多杀性巴氏杆菌，毒力基因检测结果显示该菌含有黏附素、超氧化物歧化酶等6种毒力基因。分离菌株药敏试验表明，其仅对利福平、头孢哌酮、阿莫西林、呋喃妥因、头孢噻吩、大观霉素6种药物敏感，对其余18种药物均耐药。动物致病性试验结果显示该菌株对昆明小鼠具有较强致病性，8只实验组昆明小鼠在接种后72 h内发病死亡5只，其余3只发病后耐过康复。本研究结果可为生猪养殖过程中A型多杀性巴氏杆菌病的防治提供参考。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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