共查询到19条相似文献，搜索用时 187 毫秒
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马鼻肺炎(Equine rhinopneumonitis,ER)是马属动物几种高度接触传染性疾病的总称。其病原体为亲缘关系密切的两种疱疹病毒-马疱疹病毒1型(EHV-1)和马疱疹病毒4型(EHV-4)。EHV-1和EHV-4在全世界广泛分布,并对所有年龄和种类的马以及其它马科动物的健康构成严重的威胁。世界动物卫生组织将马鼻肺炎列为B类动物疫病。多年以来,马鼻肺炎一直对世界养马业构成威胁。在大量饲养马匹进行传统耕作或以之作为农业经济一部分的国家中,EHV-1和EHV-4这两种病毒感染呈地方性流行。从马以外的其它马属动物,如斑马、亚洲野驴和驴,已经分离到与EHV… 相似文献
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马鼻肺炎（ER）是由马疱疹病毒引起的马属动物传染病。由2个亲缘密切疱疹病毒，即马疱疹病毒l型（EHV-1）和马疱疹病毒4型（EHV-4）引起。EHV-1又称马流产病毒，EHV-4又称呼吸道感染病毒。 1 病原马病毒性流产的病原为马疱疹病毒1型（EHV-1），马呼吸道感染病毒（又称马鼻肺炎病毒）为马疱疹病毒4型（EHV-4），属疱疹病毒科甲疱疹病毒亚科的成员。 相似文献
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为建立马疱疹病毒Ⅰ型(EHV-1)的检测方法,本研究以EHV-1 gB基因的一段保守区域(1207 bp～1509 bp)作为检测的目的片段设计引物,通过对其反应条件的优化,建立了特异性检测EHV-1的SYBR Green I 荧光定量PCR方法.实验结果表明:该方法检测目的基因的灵敏度下限为10拷贝/μL,比常规PCR方法高100倍;与马疱疹病毒4型(EHV-4)及其他马传染病病原体无交叉反应;组内及组间的变异系数均小于2％.该方法检测速度快及高敏感性的特点为马鼻肺炎的防制提供了有力保障,同时也为进一步开展马鼻肺炎相关的研究提供了有效的辅助检测方法技术. 相似文献
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马疱疹病毒Ⅰ型,目前已公认为马鼻肺炎病的病原,关于本病的流行病学,临床症状,病理变化,在国内外的文献中已有些阐述,但驴能否感染本病,尚不清楚。1980年,本所马鼻肺炎研究组的同志从两个马场的马流产胎儿中,用乳金黄地鼠肾原代单层细胞培养,分离出病毒,经实验证实为马疱疹病毒Ⅰ型,将其培养物接种七头妊驴,除4号妊驴正产虚弱的病驴,在产后20小时死亡外,其余妊驴全部流产。经我们对所 相似文献
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马鼻肺炎炳霉是引起原发性异肺炎和妊马继发流产的一种疱疹病毒。本病在三十年代初由Dimock氏等最先在美国肯塔基州发现。当时,曾命名为“马流产病毒”。不久, 相似文献
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The equine herpesviruses are major infectious pathogens that threaten equine health. Equine herpesvirus 4 (EHV-4) is an important equine pathogen that causes respiratory tract disease, known as rhinopneumonitis, among horses worldwide. EHV-4 genome manipulation with subsequent understanding of the viral gene functions has always been difficult due to the limited number of susceptible cell lines and the absence of small-animal models of the infection. Efficient generation of mutants of EHV-4 would significantly contribute to the rapid and accurate characterization of the viral genes. This problem has been solved recently by the cloning of the genome of EHV-4 as a stable and infectious bacterial artificial chromosome (BAC) without any deletions of the viral genes. Very low copy BAC vectors are the mainstay of present genomic research because of the high stability of inserted clones and the possibility of mutating any gene target in a relatively short time. Manipulation of EHV-4 genome is now feasible using the power of BAC technology, and should aid greatly in assessing the role of viral genes in the virus-host interaction. 相似文献
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Equine Herpesvirus 1 Infection in Mares Vaccinated with a Live-virus Rhinopneumonitis Vaccine Attenuated in Cell Culture 
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下载免费PDF全文 M. D. Eaglesome J. N. R. Henry J. D. McKnight 《The Canadian veterinary journal. La revue veterinaire canadienne》1979,20(5):145-147
Vaccination, in July and again in either November or December 1976, of 55 pregnant Standardbred mares with a live-virus rhinopneumonitis vaccine attenuated in cell culture failed to protect some mares from infection with equine herpesvirus 1. From 1976-12-08 to 1977-03-08, 33 mares foaled healthy foals, 16 mares foaled dead foals or live foals which died usually within 48 hours and six mares aborted. Gross and histological examinations and virus isolation studies confirmed that equine herpesvirus 1 caused 18 of the 22 neonatal deaths, stillbirths or abortions. 相似文献
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Varrasso A Dynon K Ficorilli N Hartley CA Studdert MJ Drummer HE 《Australian veterinary journal》2001,79(8):563-569
OBJECTIVE: To develop and validate specific, sensitive and rapid (< 8 hour) diagnostic tests using polymerase chain reaction (PCR) for the diagnosis of abortion and respiratory disease caused by equine herpesvirus 1 (EHV1; equine abortion virus) and EHV4 (equine rhinopneumonitis virus). DESIGN: Primer sets based on nucleotide sequences encoding glycoprotein H (gH) of EHV1 and gB of EHV4 were designed and used in single round and second round (seminested) PCRs, and in a multiplex PCR for the diagnosis of EHV1 and EHV4 infections. METHODS: Oligonucleotide primers were designed for each virus, PCR conditions were defined and the specificity and sensitivity of the assays were determined. The tests were applied to tissue samples from aborted equine foetuses and to nasopharyngeal swabs from horses with acute febrile respiratory disease. RESULTS: Individual single round and a second round (seminested) EHV1 and EHV4 PCRs were specific in that EHV1 primers amplified all (n = 30) EHV1 isolates and did not amplify EHV4. Similarly EHV4 primers amplified all (n = 6) EHV4 isolates and did not amplify EHV1. Both PCRs were sensitive in that the first round EHV1 PCR detected 1220 molecules of EHV1 plasmid DNA and the first round EHV4 PCR detected 7280 molecules of EHV4 plasmid DNA. The EHV1 second round PCR was 100 times more sensitive in that it detected 12 molecules of EHV1 DNA and the EHV4 second round PCR was 1000 times more sensitive in that it detected 8 molecules of EHV4 DNA. There was a high correlation between detection of EHV1 by virus isolation and PCR when tissue samples from 71 aborted foetuses were examined; all samples positive by virus isolation were positive by PCR. Similarly the EHV4 PCR was at least as sensitive as virus isolation when applied to nasaopharyngeal swabs from horses with respiratory disease in that all samples positive by virus isolation were also positive by PCR. CONCLUSION: Individual single round and second round (seminested) PCRs and a seminested multiplex PCR were developed that enabled reliable, rapid detection of EHV1 and EHV4 in aborted foetal tissues and nasopharyngeal swab samples. 相似文献
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Isolation of Herpesvirus from Equine Leukocytes: Comparison with Equine Rhinopneumonitis Virus 总被引：1，自引：1，他引：0 下载免费PDF全文
下载免费PDF全文 An agent which possessed the properties of herpesviruses was isolated from the leukocytes of 71 out of 80 (88.7%) apparently normal Iowa horses. It was ether- and heat-sensitive, DNA type, and produced type-A intranuclear inclusion bodies in cell cultures. Electron micrographs revealed a virion of typical herpesvirus structure. Leukocyte isolate virus could be differentiated from equine rhinopneumonitis virus (ERV) by serum neutralization, by growth differences in rabbit kidney cells, and by fluorescent antibody staining. Specific neutralizing antibody against this agent was found in a pooled serum sample from normal horses and in the serums of herpesvirus carrier horses. Serum from a mare inhibited growth of both ERV and leukocyte viral isolates. Normal sheep, calf, and rabbit serum did not neutralize either virus. 相似文献
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Equine herpesvirus abortion in Australia 1977 to 1982 总被引：1，自引：0，他引：1
M Sabine C Feilen L Herbert R F Jones S W Lomas D N Love J Wild 《Equine veterinary journal》1983,15(4):366-370
Until 1977 no case of abortion caused by equine herpesvirus 1 (EHV1) had been recorded in Australia although the virus, called equine rhinopneumonitis virus, had been known to have been present at least since 1962. Outbreaks of EHV1 abortion occurred in New South Wales in 1977 and in 1981. Sporadic cases of EHV1 abortion had been confirmed in some parts of Australia each year since 1975. It was concluded that an abortigenic subtype of EHV1 had been introduced to Australia in 1977 and that the previously endemic respiratory subtype occasionally caused abortion. Virus isolation in a variety of cell cultures and histopathological examination of tissue were shown to be satisfactory methods of diagnosis of EHV1 abortion. Lung proved to be the specimen of choice. Slight serological differences between "abortigenic" and "respiratory" subtypes of EHV1 were found in cross neutralisation tests. A serological survey of 219 Sydney horses of various ages revealed that most yearlings had already acquired neutralising antibody to both subtypes. 相似文献
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P. B. Fretz L. A. Babiuk B. McLaughlin 《The Canadian veterinary journal. La revue veterinaire canadienne》1979,20(2):58-61
The serological results from this study clearly show that both equine influenza and equine rhinopneumonitis viruses were present during spring and autumn epidemics of respiratory disease on Western Canadian racetracks. Approximately 11% of the horses showed significant convalescent titres to influenza while 9% showed significant convalescent titres for equine viral pneumonitis. It was noted in our study a positive vaccination history corresponded with a reduction in the severity of the respiratory infection. 相似文献
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为筛选马鼻肺炎（equine rhinopneumonitis,ER）基因缺失减毒活疫苗的候选毒株,本研究参考GenBank中EHV-1（登录号：KF644579.1）目的基因序列设计同源臂引物,以本地区流行株XJ2015株DNA为模板PCR扩增gE基因同源臂gEH1、gEH1,以EGFP表达盒（CMV+EGFP+polyA）为标记基因,酶切后依次连接至载体pUC-19,成功构建重组质粒pUC-gEH1H2-EGFP。将XJ2015基因组与质粒pUC-gEH1H2-EGFP共转染至RK-13细胞进行同源重组,以EGFP为标记进行gE基因缺失毒株的筛选及纯化,并测定重组毒株效价。结果显示：经5轮荧光噬斑纯化、PCR及测序鉴定,成功获取一株携带EGFP基因的重组毒株XJ2015-△gE-EGFP,且重组毒株效价（107.1TCID50/0.1 mL）较原毒株（108.8TCID50/0.1 mL）下降约101.7TCID50/0.1 mL。采用同源重组技术成功构建了1株马疱疹病毒1型流行株gE基因缺失突变株,为未来筛选马鼻肺炎基因缺失弱毒疫苗奠定了基础。 相似文献
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1. Isolation and Characterisation of Equine Rhinopneumontis Virus and Other Equine Herpesviruses from Horses 总被引：2，自引：0，他引：2
M. J. Studdert M.V.Sc. Ph.D. A. J. Turner M.V.Sc. J. E. Peterson B.V.Sc. 《Australian veterinary journal》1970,46(3):83-89
Equine herpesviruses (EH viruses) were isolated from 9 horses in three separate outbreaks of respiratory disease. The pattern of disease in the three stables is described and evidence is presented that some of the horses were ill, possibly as a result of recurrent infection, and that reactivation of a persistent, latent infection may have occurred. An ulcerative condition of the pharyngeal region was seen in some of the horses with EH virus infection.
The cytopathogenicity for equine foetal kidney cells of the 9 EH viruses varied considerably. One isolate, EH 39 virus, which was recovered from an acute, upper respiratory tract infection, was rapidly cytopathic for equine foetal kidney cell cultures and was shown in neutralisation tests to be identical with, or closely related to equine rhinopneumonitis virus (EH virus type 1) that is associated with acute respiratory disease and abortion in other countries. More slowly cytopathic isolates were recovered from mild to subclinical upper respiratory tract infections. Evidence is presented that the property of slow cytopathogenicity is probably related to the tendency of these viruses to remain cell associated. Slowly cytopathic isolates were recovered from the nasal cavity of horse 89 on two occasions 79 days apart. One of the eight slowly cytopathic isolates, EH 86 virus, was shown to be antigenically distinct from equine rhinopneumonitis virus (EH 39 virus). 相似文献
The cytopathogenicity for equine foetal kidney cells of the 9 EH viruses varied considerably. One isolate, EH 39 virus, which was recovered from an acute, upper respiratory tract infection, was rapidly cytopathic for equine foetal kidney cell cultures and was shown in neutralisation tests to be identical with, or closely related to equine rhinopneumonitis virus (EH virus type 1) that is associated with acute respiratory disease and abortion in other countries. More slowly cytopathic isolates were recovered from mild to subclinical upper respiratory tract infections. Evidence is presented that the property of slow cytopathogenicity is probably related to the tendency of these viruses to remain cell associated. Slowly cytopathic isolates were recovered from the nasal cavity of horse 89 on two occasions 79 days apart. One of the eight slowly cytopathic isolates, EH 86 virus, was shown to be antigenically distinct from equine rhinopneumonitis virus (EH 39 virus). 相似文献
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K DYNON A VARRASSO N FICORILLI SA HOLLOWAY GH REUBEL F LI CA HARTLEY MJ STUDDERT HE DRUMMER 《Australian veterinary journal》2001,79(10):695-702
OBJECTIVE: To develop rapid (< 8 hour) tests using polymerase chain reaction (PCR) for the diagnosis of equine herpesvirus 3 (EHV3; equine coital exanthema virus), equine gammaherpesviruses 2 (EHV2) and EHV5, equine adenovirus 1 (EAdV1), EAdV2, equine arteritis virus (EAV), equine rhinitis A virus (ERAV; formerly equine rhinovirus 1) DESIGN: Either single round or second round (seminested) PCRs were developed and validated. METHODS: Oligonucleotide primers were designed that were specific for each virus, PCR conditions were defined and the specificity and sensitivity of the assays were determined. The application of the tests was validated using a number of independent virus isolates for most of the viruses studied. The PCRs were applied directly to clinical samples where samples were available. RESULTS: We developed a single round PCR for the diagnosis of EHV3, a seminested PCR for EHV2 and single round PCRs for EHV5, EAdV1, EAdV2 and RT-PCRs for EAV and ERAV. The PCR primer sets for each virus were designed and shown to be highly specific (did not amplify any recognised non-target template) and sensitive (detection of minimal amounts of virus) and, where multiple virus isolates were available all isolates were detected. CONCLUSION: The development and validation of a comprehensive panel of PCR diagnostic tests, predominantly for viruses causing equine respiratory disease, that can be completed within 8 hours from receipt of clinical samples, provides a major advance in the rapid diagnosis or exclusion diagnosis of these endemic equine virus diseases in Australia. 相似文献