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鹦鹉喙羽病(PBFD)是鹦鹉目前最常见的疾病,对鹦鹉养殖业危害极其严重。根据鹦鹉喙羽病毒(PBFDV)基因片段的克隆和序列分析,设计合成1对特异性引物,以CP基因为模板,经PCR扩增获得830 bp的核苷酸DNA,并用DIG标记DNA,制备用于检测PBFDV的特异性核酸探针。用该核酸探针对疑似感染PBFDV的鹦鹉病料进行斑点杂交检测,并对鉴定为阳性的PBFDV进行全基因组扩增和测序分析。结果显示,利用PCR结合斑点杂交技术检测PBFDV,特异性强、敏感度高,具有可重复性。鉴定为阳性的2株PBFDV全基因组序列之间同源性为100%,与已报道序列的同源性为81.5%～98.9%。本研究为我国开展PBFDV感染的分子流行病学调查和临床诊断提供了一种敏感、特异的检测方法。 相似文献
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2018年山东省某养殖场发生大量幼龄鹦鹉死亡事件,疑似为病毒感染。为探寻病因,开展了该场及省内另外两个鹦鹉养殖场的流行病学调查。利用PCR技术对临床样品中提取的DNA或RNA进行检测,结果发现新城疫与禽流感病毒均呈阴性,而禽多瘤病毒(APV1)与鹦鹉喙羽病病毒(PBFDV)呈现为强阳性,由此推断此3个鹦鹉养殖场存在APV1和PBFDV感染,平均病毒检出率分别为67.9%与71.4%,共感染检出率率为58.0%。对阳性样品进化全基因分析发现:区域内的PBFDV流行毒株同源性高,与该地区早期报道的毒株亲缘关系较为接近;APV1的VP1基因同源性较高,与欧洲分离毒株亲缘关系较为接近,说明我国流行的APV1或来源于进口鹦鹉。本研究警示,需要加强鹦鹉疾病防控,严格鹦鹉进出口检疫,并制定和健全标准的鹦鹉病检疫程序。 相似文献
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为了解2017—2018年鹦鹉喙羽病在福建省某些地区鹦鹉中的流行情况,收集了福建省部分地区298份鹦鹉粪便样品,采用PCR方法进行粪便样品检测,对其中鹦鹉喙羽病病毒检测呈阳性的5个地区的样品进行衣壳蛋白(Cap)基因测序后比较其同源性,绘制系统进化树,分析氨基酸序列,并通过生物信息学及序列分析软件预测Cap蛋白二级结构及B细胞抗原表位。结果显示:鹦鹉喙羽病病毒的平均阳性率为41.28%,福州市某动物救助站和福州动物园的阳性率较高,分别为65.17%和64.29%,其次为三明动物园、福州市花鸟市场和福州市某鹦鹉繁殖基地,南平动物园的阳性率最低;所测5个毒株Cap基因与GenBank中新西兰株(AY518913)亲缘关系较近;Cap蛋白有丰富的二级结构和多处抗原指数较高的区域,具有潜在的B细胞抗原表位,位于5~27、110~127和141~153位氨基酸残基或其附近。研究结果对防控鹦鹉喙羽病,保障鹦鹉健康养殖有重要参考意义。 相似文献
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翁善钢 《广东畜牧兽医科技》2012,37(2):1-4
鹦鹉常见的DNA病毒性疾病有鹦鹉喙羽病、禽多瘤病毒感染、鹦鹉疱疹病毒感染、鹦鹉腺病毒感染、痘病毒感染、乳头瘤病毒感染等。本文对这些疾病的病原学、流行病学、临床症状以及诊断方法等进行了综述。 相似文献
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鹦鹉喙羽症病毒(PBFDV)是圆环病毒科圆环病毒属的成员,可引起多个品种鹦鹉的急性死亡或者羽毛脱落和喙变形。病毒基因组中的ORF1和ORF2分别编码病毒复制相关蛋白(Rep protein)和衣壳蛋白(Cap protein)。Cap蛋白是圆环病毒的主要结构蛋白,也是其主要的免疫保护性蛋白。本试验应用PCR技术选择性扩增PBFDV Cap蛋白抗原表位集中区域的基因片段,并将其克隆入原核表达载体pGEX-6p-1中,诱导表达截短的Cap蛋白。SDS-PAGE及质谱鉴定结果显示,融合表达的CapT蛋白约45 kDa,切胶纯化后的蛋白浓度约1. 6 mg/mL。该Cap蛋白的成功获得为PBFDV治疗用阳性血清制备及亚单位疫苗的开发奠定了必要的物质基础。 相似文献
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鹦鹉喙羽病是由暂划入圆环病毒科的大小为１４～１７ｎｍ、呈二十面体对称的、核酸大小为１．７～２．０ｋｂ的单链ＤＮＡ无囊膜病毒引起的１种疾病,许多品种的鹦鹉均可感染该病,其特征性临床症状为羽毛对称性萎缩、脱落及某些风头鹦鹉喙变形。１９９５年Ｒｉｔｃｈｉｎｅ等报道该病的临床表现型有最急性、急性和慢性３种。Ｌａｔｉｍｅｒ等（１９９２）报道感染鹦鹉喙羽病病毒（ＰＢＦＤＶ）的鹦鹉由于其法氏囊和胸腺常出现病变,可能导致获得性免疫缺陷,从而易继发感染细菌、衣原体、病毒、真菌和原虫等病原体。ＰＢＦＤＶ可引起鹦鹉羽… 相似文献
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Avian polyomavirus (APV) and psittacine beak and feather disease virus (PBFDV) are the most common viral diseases of psittacine birds. In Taiwan, however, the existence of these viruses in psittacine birds has not been established. Polymerase chain reaction (PCR) methodology was therefore employed to ascertain whether APV and PBFDV genomes were present in isolates from psittacine birds of Taiwan. A total of 165 psittacine birds belonging to 22 genera were examined between 2002 and 2005. Findings revealed an APV-positive rate of 15.2%, a PBFDV-positive rate of 41.2%, and an APV/PBFDV dual infection rate of 10.3%. After cloning and sequencing, sequences of the PCR products were compared with sequences obtained from GenBank. For APV, the nucleotide identity among VP1 and t/T antigen coding regions ranged from 97.5% to 100% and 97.6% to 100%, respectively. For PBFDV, the nucleotide identity of ORF V1 and ORF C1 sequences ranged from 92.2% to 100% and 83.3% to 100%, respectively. The derived amino acid sequence alignment for PBFDV ORF V1 fragments revealed the conservation of two replication motifs and of the nucleotide binding site motif. In PBFDV, six of 42 deduced positions in the ORF C1 amino acid sequence were considered hypervariable. The established phylogenetic trees based on the four genome fragments examined in this study did not allow the assignment of particular APV or PBFDV nucleotide sequences to distinct avian species. 相似文献
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Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes. 相似文献
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A E Jergens T P Brown T L England 《Journal of the American Veterinary Medical Association》1988,193(10):1292-1294
Psittacine beak and feather disease syndrome was diagnosed in an adult sulfur-crested cockatoo with a history of chronic, progressive feather loss and beak necrosis. A definitive diagnosis was made on the basis of clinical signs and the observation of intracytoplasmic and intranuclear inclusions in involved feather follicular epithelium. Psittacine beak and feather disease syndrome develops in a variety of psittacine species and usually has a progressive and irreversible clinical course. Symmetric feather loss with replacement by severely dystrophic plumage is the salient clinical finding. Beak elongation and breakage also may be found. Treatment of diseased birds remains palliative and consists of a controlled environment, balanced nutrition, antibiotics, and autogenous vaccines. Avian practitioners should include psittacine beak and feather disease syndrome as a potential cause for pathologic feather loss in caged birds. 相似文献
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K S Latimer P M Rakich W L Steffens I M Kircher B W Ritchie F D Niagro P D Lukert 《Veterinary pathology》1991,28(4):300-304
The nature of feather inclusions was characterized in 32 psittacine birds (30 cockatoos, one peach-faced lovebird (Agapornis roseicollis), and one red-lored Amazon parrot (Amazona autumnalis autumnalis] with naturally-acquired psittacine beak and feather disease. Intranuclear inclusions within feather epithelial cells and intracytoplasmic inclusions within macrophages in the feather epithelium and pulp cavity contained psittacine beak and feather disease viral antigen when stained by the avidin-biotin complex immunoperoxidase technique. Ultrastructurally, inclusions were observed primarily within macrophages and to a lesser extent within epithelial cell nuclei. Macrophage inclusions appeared as paracrystalline arrays of viral particles. Intranuclear inclusions were less well defined, although scattered viral particles were present. Intracytoplasmic and intranuclear particles in ultrastructural preparations were identified by colloidal gold labeling as psittacine beak and feather disease virus. Feather epithelium was more frequently and severely involved in the disease process than was adjacent follicular epithelium. Plucked feathers with an intact epidermal collar and feather epithelium were preferred to follicular biopsies for histopathologic examination. 相似文献
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A universal PCR assay was designed that consistently detected psittacine beak and feather disease virus (BFDV) in psittacine birds affected with psittacine beak and feather disease (PBFD) from different geographic regions across Australia. Primers within open reading frame 1 (ORF1) of the BFDV genome consistently amplified a 717 bp product from blood and/or feathers of 32 birds with PBFD lesions. The PCR did not amplify a product from the feathers or blood from 7 clinically normal psittacine birds. Primers based on regions outside of ORF1 did not consistently produce a PCR product, suggesting there was some genomic variation outside ORF1. The amplified ORF1 PCR products of 10 BFDV isolates, from different psittacine species and from various regions around Australia, were cloned and comparative DNA sequence analysis demonstrated 88-99% of the ORF1 fragments. The derived amino acid sequences of the amplified ORF1 fragments demonstrated similar identity between all 10 isolates. Within ORF1, there was complete conservation of the putative nucleotide binding site and marked conservation of 2 other motifs previously identified as essential components of the replication-associated proteins of other circoviruses and geminiviruses. 相似文献
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SUMMARY A haemagglutination inhibition assay was used to detect antibody to psittacine beak and feather disease virus in sera from wild sulphur crested cockatoos (Cacatua galerita), galahs (Eolophus roseicapillus), short-billed corellas (Cacatua sanguinea), eastern long-billed corellas (Cacatua tenuirostris) and other psittacine birds in New South Wales. The seroprevalence of psittacine beak and feather disease ranged from 41% to 94% in different flocks, indicating infection with the virus is widespread in wild populations. 相似文献
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Rahaus M Wolff MH 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2003,50(8):368-371
Psittacine beak and feather disease (PBFD) is the most common viral disease of wild and captive psittacine birds. Here, we designed the first survey to investigate the existence of subclinical infections and the distribution of the causative agent named beak and feather disease virus (BFDV) inside the population of captive psittacine birds in Germany. DNA was isolated from feathers of 146 symptom-free birds from 19 different genera (all psittaformes) taken from 32 independent breeders from all over Germany. The presence of BFDV was analysed by performing polymerase chain reaction assays. Fifty-eight (39.2%) samples were found to be positive for BFDV. As expected, there was no significant predominance of one sex to be infected with BFDV. 相似文献
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K S Latimer W L Steffens P M Rakich B W Ritchie F D Niagro I M Kircher P D Lukert 《Journal of the American Veterinary Medical Association》1992,200(5):707-710
Cryptosporidiosis was diagnosed in 4 cockatoos with psittacine beak and feather disease. Three of the birds had cryptosporidiosis confined to the epithelium covering the bursa of Fabricius. One bird had generalized parasitism of the small intestine, large intestine, and bursal epithelium. All of the birds had intermittent to protracted diarrhea before death. Presumably, acquired immunodeficiency from psittacine beak and feather disease promoted establishment of cryptosporidiosis and other secondary diseases including septicemia, peritonitis, chlamydiosis, and mycotic ventriculitis. 相似文献
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The pathology of psittacine beak and feather disease 总被引：2，自引：0，他引：2
Psittacine beak and feather disease is characterised by loss of feathers, abnormally shaped feathers and overgrowth and irregularity of the surface of the beak. The disease occurs in a number of psittacine species including the Sulphur-crested Cockatoo, Lovebirds , Budgerigars and Galahs . The abnormal appearance of feathers and beak is due to a dystrophic process within the epidermis of the feather and beak. The process consists of epidermal cell necrosis, epidermal hyperplasia and hyperkeratosis. Many of the feather abnormalities are due to retention of a hyperkeratotic feather sheath. A characteristic microscopic finding is the presence of macrophages containing purple intracytoplasmic inclusions in affected epidermis and feather pulp. The inclusions consist of aggregates of particles 17 to 22 nm in diameter. Similar but smaller inclusions occur in epidermal cells. In addition, non-suppurative inflammation occurs in the feather pulp. The findings are suggestive of a viral infection. 相似文献