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1.
为了解东莞市猪旋毛虫的感染情况,2015年在东莞市15个镇(街)屠宰场采集225份血清,应用ELISA方法进行旋毛虫检测,结果在一个屠宰场中检出一份猪旋毛虫抗体阳性,阳性率为0.44%,结果表明东莞市屠宰场的猪可能存在猪旋毛虫的感染,建议采取综合防控措施防止人感染猪旋毛虫。 相似文献
2.
为了解我国部分地区屠宰生猪旋毛虫和弓形虫感染情况,2019年在广东（5个）、山东（4个）、云南（6个）等省份的15个大型、中小型生猪屠宰场,采集猪膈肌样品315份,用PCR方法进行旋毛虫和弓形虫病原学检测;在以上3省15个大型、中小型生猪屠宰场（每省5个）,采集血清样品254份,用ELISA方法进行旋毛虫和弓形虫血清学检测。结果显示：经PCR检测,采样地区所有膈肌样品均为旋毛虫阴性,仅在云南省120份膈肌样品中检出1份弓形虫阳性;经ELISA检测,采样地区血清样品的旋毛虫和弓形虫抗体阳性率分别为1.97%和2.36%,不同省份的阳性率分布不一致,中小型屠宰场阳性率均高于大型屠宰场。结果表明,广东、山东、云南等省份屠宰生猪的旋毛虫和弓形虫携带率极低,基本可以保证猪肉的产品安全;部分地区屠宰生猪存在一定的弓形虫感染抗体,尤其是中小型屠宰场,表明此类猪群需要加强饲养环节的弓形虫感染控制。本研究为保障猪肉食品安全及饲养环节的猪旋毛虫和弓形虫感染控制提供了依据和指导。 相似文献
3.
用快速酶联免疫吸附法(FAST—ELISA)对全市七个县(市、区)2002-2006年的主要屠宰场采集的8201头份猪血清进行旋毛虫抗体检验,结果2002～2006年旋毛虫抗体阳性率分别为16.8%、14.7%、52.3%、23.1%、20.5%,平均阳性率为25.9%。同时,对上述血清ELISA检测阳性猪的膈肌脚肉样2128份进行旋毛虫压片镜检和集样消化法检测,结果均未发现旋毛虫体。 相似文献
4.
为了解河南省猪群伪狂犬野毒感染情况,2020年对来自665个场次的20 147份猪血清样品进行了伪狂犬病毒gE抗体检测,平均个体阳性率和场群阳性率分别为20.93%和47.97%。按不同场群对监测结果进行分析显示,种猪场、商品代猪场、散养户和屠宰场gE抗体个体阳性率依次升高,分别为14.78%、21.51%、23.08%和24.71%;种猪场场群阳性率明显低于商品代猪场、散养户和屠宰场。按不同季节对监测结果进行分析显示,春季、夏季和冬季猪伪狂犬病毒gE抗体个体阳性率显著高于秋季,且春季和冬季场群阳性率显著高于夏季和秋季。由此可知,2020年河南省猪群伪狂犬野毒感染情况较为严重,猪群尤其是种猪场的疫病净化工作不容松懈,同时,在春季和冬季等高发时期应加强防控。 相似文献
5.
目的为调查研究20世纪90年代以来南阳地区旋毛虫发病现状,感染率,流行动态,流行病学特点。方法自2003年1月-2005年12月,应用河南省农科院生物技术研究所研制开发的具有特异、敏感、快速、简单、易存、实惠等优点的旋毛虫快速诊断试纸条,对南阳地区4个平原县(市、区)(宛城区、卧龙区、新野县、邓州市)和1个山区县内乡3 788头猪进行血清学检测和屠宰猪肌肉镜检,对南阳市郊区狗、猫、鼠和食肉昆虫四种动物共计132只分别进行血清学检测和肌肉目测镜检,将旋毛虫阳性的狗、猫、鼠和食肉昆虫分别人工喂猪做感染试验,并进行猪肉泔水、狗肉泔水、牛、羊肉泔水饲喂试验检测泔水中旋毛虫感染程度。结果南阳地区猪旋毛虫血清学阳性率为2.93%,目测镜检的阳性率为2%;狗血清学阳性率为12.5%,镜检阳性率12.5%;猫血清学阳性率50%,镜检阳性率为33.33%;鼠血清学阳性率为10.3%,镜检阳性率为5.88%;食肉昆虫血清学阳性率为4.76%。感染试验中,猪受到感染的情况分别为33.3%、66.7%、100%和0。测定草食兽未发现有旋毛虫感染。泔水试验结果:狗肉泔水感染率为10%,猪肉泔水感染率为50%,牛、羊肉泔水未发生感染。结论市区比县发病率低,猪肉与狗肉泔水喂猪是当地猪感染旋毛虫的主要来源,南阳地区的保虫宿主主要是猪,山区猪旋毛虫超过平原地区,应用旋毛虫快速诊断试纸条检测旋毛虫比传统的目测镜检检测高出近1个百分点。 相似文献
6.
为了解和对比云南边境地区和其他地区2018-2020年猪繁殖与呼吸综合征(PRRS)免疫保护水平，疫情发生风险，2018-2020年在云南与缅甸、老挝、越南接壤的14个边境县和其他地区的规模场、散养户采集猪血清，应用猪繁殖与呼吸综合征病毒(PRRSV)ELISA抗体检测方法进行病毒抗体检测。检测结果显示：2900份猪血清样品中，阳性样品2179份，群体阳性率为75.14%(2179/2900),S/P平均值为1.0510±0.7484,变异系数为70.77%。各县PRRSV抗体阳性率在50%～90%之间，并且不同地区存在差异。抗体阳性率最高为孟连91.33%(274/300)。不同阶段猪群PRRSV平均抗体水平存在差异，哺乳仔猪抗体阳性率最低为48.14%(297/617),育肥猪抗体阳性率最高87.30%(949/1087)。入境猪群和境内猪群抗体阳性率差异显著(P<0.01),养殖场，屠宰场，交易市场猪群PRRSV抗体阳性率要高于自然散养户。本研究结果表明：猪群PRRSV抗体水平在云南边境地区整齐度不高，且地区间存在差异，入境猪群有传播疫情的风险，因此可依据边境地区的养殖特点... 相似文献
7.
为进一步摸清湖南省猪群中猪圆环病毒2型（PCV2）的感染情况和危害程度,控制该病在湖南省的流行,应用ELISA方法对湖南省8个地区的526份血样进行PCV2抗体检测,结果抗体阳性率为57.41%。其中母猪抗体阳性率为72.4%,公猪抗体阳性率为51.92%,育肥猪抗体阳性率为61.46%,仔猪抗体阳性率为47.34%。可见,PCV2在湖南省猪群已经普遍存在,应引起注意。 相似文献
8.
为了解甘肃省猪日本脑炎病毒(JEV)的感染情况,采用间接ELISA法对甘肃省部分地区的1258份猪血清样本进行了猪日本脑炎抗体检测。结果显示,抗体总阳性率为73.3%,其中3月龄以下抗体阳性率为63.7%,3月龄以上为78.1%,且抗体滴度高于3月龄以下猪群,通过t检验,二者阳性率存在显著性差异。不同地区中,陇南、天水为最高,甘南最低。甘肃省猪群存在JEV感染,且感染率较高。 相似文献
9.
为了解东莞市猪戊型肝炎的流行情况,2015年在东莞市15个镇(街)屠宰场采集225份血清,应用ELISA方法进行检测,结果为屠宰场猪戊型肝炎抗体阳性率为61.33%,结果表明东莞市屠宰场猪均存在不同程度的猪戊型肝炎的感染,建议采取综合防控措施防止人感染戊型肝炎病毒。 相似文献
10.
本试验应用ELISA法对吉林地区8个PRRS未免疫猪场和9个PRRS免疫猪场的后备母猪、生产母猪和5～10周龄的断奶仔猪三个猪群进行了PRRSV抗体检测。结果表明:吉林地区8个PRRS未免疫猪场均受到PRRSV感染,猪场抗体阳性率为100%,猪群抗体阳性率在50%～75%之间,平均阳性率为62.26%;吉林地区9个PRRS免疫猪场猪群PRRSV抗体阳性率在70%～100%之间,平均阳性率为87.78%。PRRS未免疫猪场与PRRS免疫猪场猪群抗体平均阳性率相比,差异极显著(P<0.01)。 相似文献
11.
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%). Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada. 相似文献
12.
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection. 相似文献
15.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated. Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively. 相似文献
17.
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%). Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada. 相似文献
20.
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario. Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously. 相似文献
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