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1.
小鼠灌胃不同剂量的β-胡萝卜素(20、10、5 mg/kg),测定其血液生理指标及SOD、GSH-PX、CAT活性.结果表明,高、中、低剂量组的β-胡萝卜素组白细胞总数、粒细胞数和中值细胞百分数显著高于对照组(P＜0.05),高剂量的β-胡萝卜素组中值细胞百分数、血红蛋白含量、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度、血小板和血小板压积显著高于对照组(P＜0.05),中剂量的β-胡萝卜素组血清、肝组织SOD、GSH-PX、CAT活性显著高于对照组(P＜0.05),高剂量的β-胡萝卜素组出血时间、凝血时间、血清、肝组织SOD、GSH-PX活性显著低于对照组(P＜0.05).  相似文献   

2.
把 2 0只健康家兔随机分成 4组 ,第 组为对照组 ,正常饲喂 ,第 、 及 组作为中毒试验组 ,分别经口灌服 1 .5mg/ kg、2 .0 mg/ kg、1 0 .0 mg/ kg体重的氟乙酰胺溶液 ( 56 .8mg/m L) ;各中毒组家兔死亡前从心脏采取血样 ,未死亡家兔和对照组家兔在实验第 3天从心脏采取血样 ,测定血清中 AST、AL T、CK、L DH活力和 CRE、Urea含量。结果显示 :血清中 AST、AL T、CK、L DH活力明显升高 ( P <0 .0 5或 P<0 .0 1 ) ; 组家兔血清 Urea含量显著升高 ( P<0 .0 5) , 、 组与对照组差异极显著 ( P <0 .0 1 ) ;各中毒组家兔血清 CRE含量也有不同程度升高 ,但只有 组与对照组有显著性差异 ( P<0 .0 5)。实验说明家兔氟乙酰胺中毒后心脏、肝脏、肾脏等组织器官受到损伤  相似文献   

3.
文章主要研究饲粮中添加屎肠球菌制剂对泌乳中期奶牛血清生化、抗氧化和免疫指标的影响。将24头体重、胎次、泌乳量、泌乳期、体况等基本一致的荷斯坦奶牛,随机分为3组:对照组、低剂量组、高剂量组。试验预试期14 d,正试期28 d,每隔14 d于晨饲前尾静脉采集血样测定血常规、血液生化、抗氧化和免疫指标。白细胞、血红蛋白、血小板、红细胞压积、红细胞平均体积、平均血红蛋白含量、平均血红蛋白浓度、红细胞分布等血常规组间无显著影响(P0.05),但低剂量组红细胞数量显著高于高剂量组(P0.05);血清碱性磷酸酶、总胆固醇、总蛋白、血中尿素氮等指标组间无显著影响(P0.05),但高剂量组的乳酸脱氢酶显著低于对照组(P0.05);丙二醛、还原型谷胱甘肽、超氧化物歧化酶、过氧化氢酶等组间皆无显著影响(P0.05);另外,高剂量组肿瘤坏死因子显著低于对照组和低剂量组(P0.05)。综上所述,饲粮中添加屎肠球菌提高了红细胞数量和抗肿瘤因子的浓度,降低了乳酸脱氢酶的浓度,利于免疫力的提高。  相似文献   

4.
银川地区小尾寒羊血常规生理指标的测定   总被引：4，自引：0，他引：4  
通过对小尾寒羊部分血常规的测定 ,为小尾寒羊在银川地区的饲养管理和疾病的诊断治疗提供一些参考数据。主要测定项目有血红蛋白的含量、红细胞压积、红细胞计数、白细胞计数和白细胞分类计数等指标。同时与宁夏滩羊相比小尾寒羊的血红蛋白的含量、红细胞计数、白细胞计数差异极显著 (P <0 .0 1) ,红细胞压积差异显著 (P <0 .0 5 ) ,白细胞分类计数中嗜碱性白细胞、嗜酸性白细胞、幼稚型嗜中性白细胞、分叶型嗜中性白细胞和单核细胞差异极显著 (P <0 .0 1) ,杆型嗜中性白细胞和淋巴细胞差异不显著 (P >0 .0 5 )。  相似文献   

5.
为研究成年徐海鸡的血液生理生化指标,试验测定并分析了40周龄徐海鸡的各项血液生理生化指标。结果表明:1血液生理指标:徐海鸡公鸡红细胞计数(RBC)、白细胞计数(WBC)、血小板计数(PLT)、血红蛋白浓度(HGB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白(MCH)、平均红细胞血红蛋白浓度(MCHC)测定值极显著大于母鸡(P0.01),公鸡红细胞分布宽度(RDW)测定值显著大于母鸡(P0.05);2血液生化指标:徐海鸡公鸡谷草转氨酶(AST)、肌酸激酶(CK)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、葡萄糖(GLU)、钠(Na)离子测定值极显著高于母鸡(P0.01),母鸡甘油三酯(TG)、镁(Mg)离子、钙(Ca)离子测定值极显著大于公鸡(P0.01),母鸡胆碱酯酶(CHE)、钾(K)离子测定值显著高于公鸡(P0.05)。  相似文献   

6.
为了提高猪血常规测定结果的准确性,试验采集6头广西巴马小型猪血液,利用全自动血细胞分析仪测定并分析了采血量、冷藏保存时间、冷冻温度对猪血常规测定结果的影响。结果表明:采血量较大(3 mL左右)时血常规测定结果的稳定性更差;血样采集后在4℃保存4 h内测定对血常规指标没有显著影响,8 h以后测定对白细胞指标有显著影响;-20℃、-40℃、-80℃冷冻后测定血常规均会对大多数指标有极显著影响;液氮冷冻30 min对猪血液红细胞总数、血红蛋白浓度、血红蛋白含量、血小板总数等指标没有显著影响。说明测定猪血常规应采集较少量(1 mL左右)血液于4℃保存并于4 h内完成测定。  相似文献   

7.
研究复方中药添加剂对仔猪生产性能和血液生理指数的影响。试验断奶仔猪随机分为4组,每组10头,分别为对照组、低剂量组、中剂量组和高剂量组,分别在日粮中添加0、0.5%、1.0%和2.0%复方中药添加剂,饲养21 d。在第21天饲喂前空腹称质量记录体质量,计算平均日采食量(ADFI)、平均日增质量(ADG)和料重比(F/G)。前腔静脉空腹采集全血5 m L。测定全血中红细胞计数(RBC)、红细胞压积(HCT)、血红蛋白(HGB)、血小板计数(PLT)和白细胞计数(WBC)。在断乳仔猪日粮中添加0.5%、1.0%和2.0%复方中药添加剂,仔猪的平均日增质量、平均日采食量有所提高和料重比有下降趋势,但各组间差异不显著(P0.05)。低剂量组、中剂量组和高剂量组仔猪血液中白细胞数都显著高于对照组(P0.05),血液血小板数显著低于对照组(P0.05),红细胞压积各组间差异不显著(P0.05)。中剂量组和高剂量组的仔猪血液中红细胞数和血红蛋白含量显著高于对照组(P0.05)。  相似文献   

8.
本试验分别选用10头健康仔猪和附红细胞体感染仔猪,进行了血液红细胞压积、红细胞总数、血红蛋白、白细胞总数等生理指标的检测。结果显示,附红细胞体感染的仔猪红细胞压积(P0.01)、红细胞总数(P0.01)和血红蛋白(P0.01)显著低于健康仔猪,白细胞总数(P0.01)显著高于健康仔猪;附红细胞体感染的仔猪嗜中性白细胞(P0.05)、单核细胞(P0.05)显著高于健康仔猪,嗜酸性白细胞(P0.05)和淋巴细胞(P0.01)显著低于健康仔猪。结果表明,附红细胞体的侵袭引起猪血液指标的变化是猪附红细胞体病的共同特征。  相似文献   

9.
本试验选取16只3~4 kg健康泌乳家兔,通过人工造模的方法诱发其乳腺发炎,建立临床型乳腺炎模型。然后分别在造模前1 h和造模后4、12、24、36、487、2 h采集血液0.5 mL、血清1 mL,进行血常规、血生化检测和炎性细胞因子测定(ELISA)。结果显示:①外周血白细胞总数在造模后4 h显著高于造模前水平(P0.05),12~72 h内极显著高于造模前水平(P0.01);红细胞总数、红细胞压积、血红蛋白含量在造模前至造模后72 h内无显著差异(P0.05)。②血清磷离子含量、血清总胆红素含量、血清直接胆红素含量在造模前至造模后72 h内无显著差异(P0.05);血清钙离子含量在造模后4 h显著低于造模前水平(P0.05),12~72 h内极显著低于造模前水平(P0.01)。③血清TNF-α和IL-6含量造模后开始升高,12~72 h内极显著高于造模前水平(P0.01)。结果证明白细胞总数、血清钙离子含量、血清TNF-α和IL-6含量的变化可作为急性乳房炎炎症发展情况的指标。  相似文献   

10.
中度热应激对荷斯坦牛部分血液生化指标的影响   总被引：3，自引：0，他引：3  
本研究测定了中国荷斯坦牛分别在中度热应激、无热应激状态下的23个血液生化指标,结果表明:在中度热应激(THI=82.06)状态下,试验牛血液的总蛋白(TP)含量、红细胞数(RBC)、平均血红蛋白(MCH)含量、红细胞压积(HCT)与无热应激组差异显著(P＜0.05);血清肌酐(CRE)含量、胆固醇(CHO)含量、淀粉酶(AMY)含量、红细胞平均血红蛋白(MCHC)含量、白细胞数(WBC)、碱性磷酸酶(ALP)6项血液生化指标与无热应激组差异极显著(P＜0.01),其余13个生化指标两组间差异不显著.同时对结果进行了分析讨论,希望能为科学防控奶牛热应激提供参考思路和基础数据.  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
Tropical Animal Health and Production -  相似文献   

14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

18.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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