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1.
[目的]研制以核桃和花生为原料,打浆后添加到牛乳,发酵制成营养丰富的坚果酸奶。[方法]通过单因素试验和正交试验,探究坚果浆比例、蔗糖添加量、接种量、发酵时间对酸奶品质的影响,结合感官评价和酸度确定核桃花生酸奶的最优工艺参数。[结果]坚果浆占牛奶比例55%,蔗糖添加量5%,接种量3%,42℃发酵4.0 h,经后熟得到的酸奶综合评分最高,为86分,酸度为72°T。[结论]此酸奶产品口感细腻顺滑,坚果香味和奶香味浓郁。  相似文献   

2.
新疆牧区传统酸奶干酪的营养成分及脂肪酸含量分析   总被引:5,自引:3,他引:2  
本文对新疆主要牧区牧民传统方法制造的酸奶干酪营养成分及脂肪酸组成进行了分析研究。结果表明,大多数酸奶干酪样品中水分含量在5%FAT,质地干硬。pH值和总酸含量分别为3.9±0.2和5.6%±0.4。粗灰分、粗蛋白质和总脂肪含量分别为5.7%,52.0%和18.6%,其中粗脂肪含量的变化幅度较大(6.3%-31.1%)。酸奶干酪脂质中检测出近20种脂肪酸,其中油酸(C18:1)含量最高,占总脂肪酸的32.7%。不饱和脂肪酸总量的81.1%。  相似文献   

3.
本试验旨在研究在尽可能相同的生长状态下山羊和奶牛乳中乳蛋白、乳脂肪和乳中微量元素的种间差异。结果显示,山羊和奶牛乳中乳蛋白分数差异显著,山羊乳中αs1-酪蛋白含量(28.8%)显著低于奶牛乳中αs1-酪蛋白含量(P〈0.05)。虽然山羊乳和奶牛乳中总必需氨基酸含量差异不显著(P〉0.05),但氨基酸谱图存在一定的差异。两品种乳中乳脂组成显著不同,尤其是中、长链脂肪酸的含量(C4-C14),山羊乳中含量显著高于奶牛乳中含量(P〈0.05);山羊乳中n-6多不饱和脂肪酸(10.0%)、n-3多不饱和脂肪酸(51.0%)和共轭亚油酸含量(33.8%)也显著高于奶牛乳中相应的含量(P〈0.05)。  相似文献   

4.
试验旨在采用体外产气法研究抑制瘤胃细菌、真菌、原虫对添加亚油酸和亚麻酸后水牛瘤胃体外发酵参数和脂肪酸代谢的影响。体外培养底物0.5 g,精粗比为3:7,分别添加底物干物质量3%的亚油酸和3%的亚麻酸,每组设置5个重复,同时再设立4个组:对照组及抑制原虫、细菌、真菌组。体外模拟瘤胃发酵培养24 h,测定24 h产气量和气体中的甲烷(CH4)含量、瘤胃发酵液的pH、挥发性脂肪酸(VFA)、氨态氮(NH3-N)、微生物蛋白(MCP)浓度以及长链脂肪酸(LFA)组成。结果表明:①在添加亚麻酸情况下,与对照组相比,抑制细菌和原虫生长后产气量显著降低,抑制细菌和真菌生长后CH4产量显著升高,而抑制原虫生长后CH4产量显著降低(P<0.05);在添加亚油酸情况下,与对照组相比,抑制细菌、真菌或原虫生长后产气量均显著降低,且抑制原虫后CH4产量显著低于其他组(P<0.05)。②抑制细菌、真菌或原虫生长后,添加亚油酸和亚麻酸显著影响了体外瘤胃发酵液pH和MCP浓度(P<0.05),添加亚油酸对NH3-N浓度影响不显著(P>0.05)。③与对照组相比,抑制细菌、真菌或原虫生长后显著降低了乙酸、丙酸含量(P<0.05);在添加亚麻酸情况下,抑制细菌生长显著降低了丁酸含量(P<0.05);在添加亚油酸情况下,抑制细菌、真菌或原虫生长后丁酸含量显著降低(P<0.05)。④与对照组相比,在添加亚麻酸情况下,抑制细菌生长显著降低了C11:0、C12:0、C13:0、C14:0、C14:1n5、C15:1n5、C16:1n7、C16:0、C18:3n3、C18:2n6c、C18:0、C20:2n6、C20:3n6、C20:1、C20:3n3、C20:0、C21:0、C22:6n3、C22:2n6、C22:0浓度(P<0.05);在添加亚油酸情况下,抑制细菌生长显著降低了C12:0、C13:0、C14:0、C15:0、C16:1n7、C16:0、C17:0、C18:3n6、C18:3n3、C18:2n6c、C18:1n9t、C18:0、C18:2(cis-9,trans-11)、C18:2(trans-10,cis-12)、C20:2n6、C20:1、C20:0、C21:0、C22:6n3、C22:0、C23:0、C24:1n9、C24:0浓度(P<0.05)。由此可见,抑制细菌、真菌或原虫生长后,添加亚油酸和亚麻酸对体外瘤胃发酵参数、CH4产量和脂肪酸组成均能产生影响,原虫对产气量和CH4产量贡献最大,细菌对瘤胃液脂肪酸代谢影响最大。  相似文献   

5.
为探究亚油酸对反刍动物瘤胃中溶纤维丁酸弧菌脂肪酸合成的影响,试验用不同水平亚油酸培养基进行溶纤维丁酸弧菌体外厌氧培养试验,分光光度计测定菌液D600 nm值达到1.0时,在培养基中添加亚油酸,使试验1组(对照)、2、3及4组培养基亚油的浓度分别为0、2.5、5.0及7.5 mg/100 mL,培养24 h后收集菌液检测35种脂肪酸含量。结果显示,细菌代谢相关的C8:0、C11:0、C14:0、C15:0、C17:0、C17:1、C18:1(n-9) C、C18:2(n-6) C、C18:3(n-6)、C20:0、C20:2、C20:3(n-3)、C20:3(n-6)、C20:5(n-3)、C21:0、C22:0、C22:2、C22:1(n-9)及C22:6(n-3)脂肪酸随着培养基中亚油酸浓度的提高无显著变化(P>0.05);培养基中添加亚油酸使C10:0、C12:0、C16:0、C18:0、C20:4(n-6)、C23:0、C24:1合成量显著减少(P<0.05);而C15:1及C18:3(n-3)合成量在2.5 mg/100 mL亚油酸组显著增加(P<0.05);C14:1在对照组中合成量最高,在添加亚油酸的试验组中,随着亚油酸含量增加而增加;C24:0在7.5 mg/100 mL亚油酸组合成量最高。综上所述,亚油酸对于溶纤维丁酸弧菌合成脂肪酸有一定影响作用,可抑制C10:0、C12:0、C16:0、C18:0、C23:0、C14:1、C20:4(n-6)等脂肪酸的合成,C15:1、C16:1、C18:3(n-3)含量增加,但超过一定量时合成量会减少。  相似文献   

6.
本文探讨了酸奶前发酵阶段的温度、接种量、酸度与时间之关系。结果表明:发酵温度对发酵时间有极显著的影响(P<0.01),且以42℃为宜。接种量对发酵时间亦有极显著的效果(P<0.01),且以3%为佳。在各种温度和接种量条件下,发酵酸度与发酵时间之间均符合y=aebx的指数函数关系(P<0.01)。  相似文献   

7.
广西水牛乳脂肪酸四季变化规律的研究   总被引:1,自引:1,他引:0  
为了研究不同季节对水牛乳脂肪酸组成的影响,试验选取春、夏、秋、冬4个季节广西地区生鲜水牛原乳720份,采用气相色谱法测定水牛原乳脂肪酸组成和含量,以分析水牛乳脂肪酸四季的变化规律。结果显示,水牛原乳共检出38种脂肪酸,除C13:0、C15:0、C16:0和C20:4n6外,季节对其他脂肪酸含量均有显著影响(P<0.05);多不饱和脂肪酸(PUFA)总含量在四季差异不显著(P>0.05),冬季短链脂肪酸(SCFA)总含量显著高于其他季节(P<0.05),夏季长链脂肪酸(LCFA)总含量和单不饱和脂肪酸(SUFA)总含量均显著高于其他季节(P<0.05),而夏季中链脂肪酸(MCFA)总含量和饱和脂肪酸(SFA)总含量均显著低于其他季节(P<0.05);夏季ω6系列脂肪酸含量显著高于冬季(P<0.05),春、秋两季ω3系列脂肪酸总含量显著高于夏、冬季节(P<0.05);ω6与ω3系列脂肪酸比值在春季最低(4.43),其次是秋季和冬季,夏季ω6/ω3值最高(6.32)。由此可见,不同季节对水牛原乳脂肪酸组成有显著影响,欲优化脂肪酸组成应合理改善日粮结构并提高饲养管理水平。  相似文献   

8.
研究普洱茶酸奶的生产方法,通过正交试验,确定最佳的工艺条件。结果表明:提取条件是茶水比为1:10,冲泡温度为90℃,冲泡20min时所提取得普洱茶汁最佳。牛奶中添加茶汁量为3%,糖6%,菌种接种量2%,稳定剂添加量为0.05%,40℃条件下发酵4h获得的普洱茶酸奶茶香浓郁,与奶味融和较好。与普通酸奶比较有独特香味。  相似文献   

9.
山羊酸奶加工工艺研究   总被引:1,自引:0,他引:1  
山羊奶含有丰富的蛋白质、脂肪、乳糖、矿物质及多种维生素,Ca、P及某些生物活性物质(如cAMP,cGMP等)含量高于牛奶。采用响应曲面法研究了山羊酸奶的发酵温度、加糖量、球杆菌比例和接种量。结果表明:在38℃,蔗糖添加量6%,嗜热链球菌接种量1.5%,保加利亚乳杆菌接种量0.5%的条件下,山羊酸奶品质最好。  相似文献   

10.
选用佛手和脱脂奶粉为主原料,采用阿斯巴甜和木糖醇代替蔗糖,通过发酵研制出一种低热量佛手酸奶。经单因素试验和各因素正交试验,确定低热量佛手酸奶最佳配方为:佛手汁含量为3%,脱脂奶粉量为12.6%,木糖醇4%,阿斯巴甜0.02%,接种量为3%。最佳工艺参数为:均质温度为70℃,均质压力为20MPa,发酵温度为43℃,发酵时间为4.5h。本产品不仅为佛手的利用开辟了新的途径,而且其热量低的特点可以满足多种人群的需要。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

14.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

15.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

16.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

17.
The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma.  相似文献   

18.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

19.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

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