首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献，搜索用时 15 毫秒
1.
This study was conducted to evaluate the pathological changes in broilers fed a diet containing low-levels of aflatoxin (AF) and clinoptilolite (CLI) until 42 days of age. A total of 576 one-day-old Ross-308 type broiler chicks were treated with varying levels of AF and CLI (15 g kg(-1)). The gross and histopathological changes in the liver, kidneys, spleen, thymus and bursa of Fabricius were investigated and relative organ weights were calculated. Compared to controls, significant changes (P<0.05), such as slight to moderate hydropic degeneration and/or fatty change (8 cases of 10), bile-duct hyperplasia (7 of 10) and periportal fibrosis (5 of 10), were found in chicks fed 100 ppb AF-containing diet. No gross-pathological changes were observed in any treatments. The addition of CLI to the 100 ppb AF-containing diet significantly decreased the number of affected broilers and/or the severity of lesions (hydropic degeneration and bile-duct hyperplasia) in the livers (P<0.05). The addition of CLI to the AF-free diet did not produce any significant lesions compared with the controls.  相似文献   

2.
本文通过测定肉仔鸡生长性能、血清指标及器官指数,探讨葡甘露聚糖对黄曲霉毒素B1(AFB1)的吸附作用。选用180只体重接近的8日龄肉仔鸡随机分成3组,分别为基础日粮组、基础日粮 0.1mg/kgAFB1组、基础日粮 0.1mg/kgAFB1 0.1%葡甘露聚糖组,试验期21d。试验结果表明:(1)基础日粮中加入0.1mg/kgAFB1,肉仔鸡的体重、日增重、日采食量显著下降,料重比升高。血清中总蛋白、白蛋白含量显著下降,谷草转氨酶、谷丙转氨酶活性显著升高。肝脏、肾脏、心脏和脾脏肿胀,胸腺、法氏囊萎缩;(2)0.1mg/kgAFB1日粮中加入0.1%葡甘露聚糖,明显改善肉仔鸡的生长性能,以上相关指标基本恢复到正常水平。由此可知,葡甘露聚糖减轻或基本消除了AFB对肉仔鸡生长性能、组织器官的不良影响。  相似文献   

3.
The amelioration of aflatoxicosis in broiler chickens was examined by feeding two concentrations of natural zeolite (clinoptilolite). Clinoptilolite (ClI), incorporated into the diet at 1.5 and 2.5 per cent, was evaluated for the ability to reduce the deleterious effects of 2.5 mg total aflatoxin (AF) kg(-1)diet on growing broiler chicks from 1 to 21 days of age. A total of 360 broiler chicks were divided into six treatment groups [Control, AF, CLI (1.5 per cent), AF plus CLI (1.5 per cent), CLI (2.5 per cent), and AF plus CLI (2.5 per cent)] each consisting of 60 chicks. Compared to controls, the AF consuming chicks showed increases in the relative weights of liver and kidney; and gross-histopathologic hepatic lesions such as paleness, friability, diffuse hydropic degeneration and/or fatty change, bile-duct hyperplasia and periportal fibrosis. Glumerular hypertrophy, increases in the number of mesengial cells and hydropic degeneration of tubuler epithelium in kidneys of chicks fed diet AF alone were also observed. Atrophy and lymphoid depletion were seen in the thymuses and bursa of Fabricius from the chicks fed AF alone. The additions of CLI (1.5 and 2.5 per cent) to the AF -containing diet moderately (significantly in some cases) decreased the number of affected broilers and/or the severity of lesions. The addition of CLI to the AF-free diet did not produce any significant changes compared with the controls. These results suggest that CLI was effective for the protection of AF-toxication in broilers and it could contribute to a solution of the AF problem in poultry production.  相似文献   

4.
1. Toxic effects of two concentrations (0.5 and 1 mg/kg) of ochratoxin A (OTA) and attenuating effects of a toxin deactivator (Mycofix Plus(MTV INSIDE)) containing the yeast Trichosporon mycotoxinivorans on the performance (feed conversion ratio; body weight gain), serum enzymes (lactate dehydrogenase, gamma-glutamyltranspeptidase and aspartate aminotransferase) and clinico-pathomorphology of internal organs were studied in 270 one-day-old broiler chicks divided into 9 groups over a 42-d period. 2. Feed conversion ratios (FCR) in groups fed toxin deactivator were improved compared with groups receiving OTA only. An increase in the relative weight of kidney and liver was observed in groups fed 0.5 and 1 mg/kg OTA on day 42 of the experiment as compared with the control group. In contrast, relative weights of bursa of Fabricius and spleen were not significantly affected in experimental groups exposed to OTA as compared to control groups determined on days 28 and 42 of age. 3. Serum enzymes (LDH, GGT and AST) values in OTA treated groups determined on days 28 and 42 were higher than those of the control group. 4. Histopathological examination of kidney on day 42 revealed degenerative changes in the epithelial cells of the proximal convoluted tubules and massive necrosis of the proximal tubular epithelial cells. These changes were less marked in birds receiving 0.5 mg/kg OTA than in those receiving 1 mg/kg. In general, histological changes in kidneys, liver, bursa and spleen were less pronounced in birds receiving OTA and toxin deactivator concomitantly. 5. Dietary OTA at 0.5 and 1 mg/kg adversely affects FCR, increases the serum liver enzymes and induces pronounced pathomorphological and histological changes in internal organs of broiler chicks. Co-administration of OTA with deactivator attenuated the harmful effects.  相似文献   

5.
150只一日龄罗曼混合肉雏被随机分为四组,分别饲喂含维生素A为0、750、3000和6000IU的日粮。在20、30和45日龄时,每组剖检5只鸡,分离并称重免疫器官后,取材,石蜡包埋,切片,镜检。结果表明:维生素A缺乏和不足使免疫器官组织萎缩,间质结缔组织增生,上皮角化,腔上囊和胸腺的相对重量明显减少(P<0.01)。对免疫器官的损害程度是腔上囊>胸腺>脾脏>盲肠扁桃体。提高日粮中维生素A含量有提高肉雏增重、饲料报酬和免疫器官相对重量之趋势。  相似文献   

6.
1. The amelioration of aflatoxicosis in broiler chickens was examined by feeding 2 concentrations of natural zeolite (clinoptilolite). Clinoptilolite (CLI), incorporated into the diet at 15 and 25 g/kg, was evaluated for its ability to reduce the deleterious effects of 2.5 mg total aflatoxin (AF; 76.40% AFB1, 16.12% AFB2, 6.01% AFG1 and 1.47% AFG in diet on growing broiler chicks from 1 to 21 d of age. A total of 360 broiler chicks were divided into 6 treatment groups (6 replicates of 10 broilers each): control, AF, CLI (15 g/kg), AF plus CLI (15 g/kg), CLI (25 g/kg), and AF plus CLI (25 g/kg). 2. Compared to controls, the treatment had significantly decreased body weight gain from week 1 onwards. The adverse effect of AF on food consumption (8.0%) and food conversion ratio (8.3%) was also shown over the entire 21-d feeding period. 3. The addition of CLI (15 g/kg) to an AF-containing diet significantly reduced the deleterious effects of AF on food consumption and body weight gain. Food conversion ratio was also slightly improved by adding CLI (15 g/kg) to AF-containing diets. Food consumption, body weight gain and food conversion ratio values were rendered numerically intermediate between AF and control groups by the addition of CLI (25 g/kg) to the AF-containing diet. 4. The addition of CLI (both 15 and 25 g/kg) to the AF-free diet did not produce any significant changes compared with the controls, except for decreased total food consumption in the CLI (25 g/kg)-alone group. 5. These results suggest that CLI (15 g/kg) addition effectively diminished the detrimental effects of AF on the values investigated. Also, the lower dietary concentration of CLI (15 g/kg) was more effective than the greater concentration against the adverse effects of AF on the variables investigated in this study.  相似文献   

7.
1. This study was to evaluate the toxic effects of aflatoxin (AF) on growth performance of quail, and to determine the preventive efficacy of MYCOTOX (oxicinol, tymol, micronised yeast). 2. One hundred and eighty 1-d-old quail (Coturnix coturnix japonica) of both sexes were weighed and randomly divided into 4 experimental groups each with 5 replicates of 9 birds. 3. There were 4 dietary treatments: (1) control with 0 mg AF/kg diet and 0% MYCOTOX; (2) 0 mg AF/kg diet and 0.5% MYCOTOX; (3) 2.5 mg AF/kg diet and 0% MYCOTOX; (4) 2.5 mg AF/kg diet plus 0.5% MYCOTOX. The chicks were maintained on these treatments to 3 weeks of age. Quail consumed the diets and water ad libitum. 4. Body weight (BW) gains in groups receiving AF alone were the lowest at all periods. Feed intake was lowest in the group consuming the AF diet. The addition of MYCOTOX to the AF diet did not prevent or reduce the toxic effects of AF on feed intake at any time period. Feeding diets containing MYCOTOX alone did not change feed intake significantly. With the exception of the 1 to 7 d period, feed conversion of chicks fed the AF diet was similar to those of the other experimental groups. 5. Bursa of Fabricius weight decreased, whereas the relative weights of liver, kidney and spleen increased in quail consuming diets containing AF and AF plus MYCOTOX. Liver colour was normal in the control and MYCOTOX alone group, but was lighter in groups fed AF. 6. The results indicated that MYCOTOX was not effective in preventing the deleterious effects of AF.  相似文献   

8.
1. The effects of dietary aflatoxin (AF, 0.5, 1.0 and 2.0 mg/kg), ochratoxin (OA, 1.0, 2.0 and 4.0 mg/kg) or combinations of these on body weight gain, feed efficiency, organ weights and immune response were studied in broilers. 2. Significant growth depression, reduced food consumption and poor food conversion efficiency were recorded in broilers fed a diet containing the greater concentrations of AF (1 and 2 mg/kg) and OA (2 and 4 mg/kg). 3. The combination of 2 mg/kg AF and 4 mg/kg OA exerted the maximum adverse effect on growth, feed intake and feed efficiency, indicating a synergistic effect on performance. 4. AF at 2 mg/kg in the diet caused a significant increase in the relative weight of liver, whereas the relative weight of kidney was significantly increased at 4 mg/kg of OA. A significant decrease in the relative weight of the bursa of Fabricius was noted at the highest concentration of AF (2 mg/kg) and combinations of 1 and 2 mg/kg AF and 2 and 4 mg/kg OA. 5. Cell mediated immunity (CMI), in terms of mean skin thickness (MST) sensitive to dinitrochlorobenzene (DNCB), was significantly reduced in chicks given the combination of 2 mg/kg AF and 4 mg/kg OA. Haemagglutination (HA) titre against sheep red blood cells (SRBCs) peaked at 42 d of age. At 42 and 47 d of age, a significant decrease in HA titres was recorded in chicks given 4 mg/kg OA or a combination of AF (1 or 2 mg/kg) and OA (2 or 4 mg/kg). 6. AF at a dietary concentration of 1 mg/kg or more and OA at 2 mg/kg or more, either alone or in combination, caused severe reductions in growth and immune response.  相似文献   

9.
Day-old broiler chicks (n=30) were obtained from a commercial hatchery and inoculated, either orally or intracloacally, with a characterized strain of Campylobacter jejuni. At 1 hr, 1 day, and 1 wk after inoculation, broilers (n = 5) from the orally and intracloacally inoculated groups along with control birds (n=4) were humanely killed by cervical dislocation. The broilers from the control and treatment groups were aseptically opened, and the thymus, spleen, liver/gallbladder, bursa of Fabricius, and ceca were aseptically removed and individually analyzed for C. jejuni. Overall, C. jejuni was isolated after oral inoculation from 13% (10/ 75), 17% (13/75), and 28% (14/50) of the 1-hr, 1-day, and 1-wk samples, respectively. Campylobacter jejuni was isolated from 10% (4/ 40), 8% (3/40), 10% (4/40), 25% (10/40), and 40% (16/40) of the thymus, spleen, liver/gallbladder, bursa of Fabricius, and ceca samples, respectively. Following the intracloacal route of inoculation, C. jejuni was recovered from 32% (24/75), 8% (6/75), and 16% (8/50) of the 1-hr, 1-day, and 1-wk samples, respectively. Campylobacter jejuni was isolated from 5% (2/40), 5% (2/40), 5% (2/40), 45% (18/40), and 40% (16/40) of the thymus, spleen, liver/gallbladder, bursa of Fabricius, and ceca samples, respectively, for all sampling periods. Campylobacter spp. were not recovered from sample sites examined from the control broilers from trial one, trial two, or trial three samples examined after 1 hr and 1 day. However, one control sample was positive from the 1-wk sampling from repetition three; therefore, those data were omitted. The rapid movement of Campylobacter to internal organs following both oral and intracloacal inoculation may be significant, particularly if it persists in these organs as reservoirs throughout the 65-wk life cycle of breeding birds.  相似文献   

10.
Impact of BSE on Livestock Production System   总被引：1，自引：0，他引：1  
A total of 240 unvaccinated day-old broiler chicks, which had been found to be negative for antibodies against FAV-4, were divided into four groups of 60 chicks each. Group A was fed aflatoxin at 1 ppm from 7 days to 7 weeks of age. Group V was infected intra-abdominally at 14 days of age with 0.2 ml of FAV-4, having a titre of 105.5 TCID50 per 0.2 ml. The combined group AV was given the aflatoxin and infected with FAV-4. The fourth group C served as the control. More pronounced clinical signs, a higher mortality rate (56.7%), and reductions in body weight gain and in the organ to body weight ratios of the bursa and spleen were recorded in group AV. A significant (p<0.01) reduction in the HI antibody titre following vaccination against Newcastle disease, and of skin thickness in the delayed hypersensitivity test following sensitization with DNCB, indicated an additive immunosuppressive effect from aflatoxin and FAV-4 on the humoral and cell-mediated immune responses in group AV compared to groups A and V. Microscopically, marked depletion and degeneration of lymphocytes in the thymus, bursa, spleen and caecal tonsils were observed in group AV up to 5 weeks PI.  相似文献   

11.
以马立克氏病病毒（ＭＤＶ）感染１日龄肉用雏鸡，在感染后５、２５、４５ｄ采取法氏囊、脾脏、盲肠扁桃体和哈德尔腺，用彩色免疫金银染色法检查免疫器官组织中ＩｇＧ、ＩｇＭ和ＩｇＡ抗体生成细胞数量的动态变化。结果：ＭＤＶ感染雏鸡的法氏囊、脾脏和哈德尔腺中以ＩｇＧ抗体生成细胞居多，ＩｇＧ、ＩｇＭ和ＩｇＡ抗体生成细胞均较正常对照鸡显著减少；盲肠扁桃体中以ＩｇＡ抗体生成细胞居多，ＩｇＡ、ＩｇＧ和ＩｇＭ抗体生成细胞数量显著低于正常对照鸡。由此表明，ＭＤＶ感染鸡全身免疫器官和消化道、呼吸道局部粘膜体液免疫机能明显抑制。  相似文献   

12.
This study was conducted to evaluate the protective efficacy of clinoptilolite (CLI, a natural zeolite) in the prevention of toxic effects of aflatoxin (AF). A total of 576 1-day-old Ross broiler chicks were housed in six treatment groups [six replicates of 16 each; control, CLI (15 g kg(-1)diet), 50 parts per billion (ppb) AF, 50 ppb AF plus CLI, 100 ppb AF, 100 ppb AF plus CLI ] for 42 days. Compared to controls, 100 ppb AF treatment significantly decreased body weight gains of chicks. The addition of CLI to the 100 ppb AF - containing diet moderately reduced the adverse effects of AF on performances of chicks. The chicks consuming 50 ppb AF -containing diet showed no significant differences on investigated parameters compared to controls. Also, the single addition of CLI to the AF -free diet had no adverse effects in birds. These results suggest that CLI can be beneficial in broilers for protection of AF toxicity at the level above.  相似文献   

13.
王莉  陈晓  王书全 《中国畜牧兽医》2017,44(8):2354-2359
试验旨在研究天蚕素抗菌肽对817肉杂鸡生长性能和免疫功能的影响。选取1日龄817肉杂鸡900羽,随机分为5个处理组（对照组、抗生素组及Ⅰ、Ⅱ、Ⅲ组）,每组6个重复,每个重复30羽。对照组饲喂基础日粮,抗生素组在基础日粮中添加150 mg/kg金霉素,Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加100、200和300 mg/kg天蚕素抗菌肽,试验期42 d。结果显示,Ⅱ、Ⅲ组能显著提高1~21日龄817肉杂鸡的平均日增重（P<0.05）,显著降低料重比（P<0.05）;显著提高21、42日龄817肉杂鸡的胸腺、法氏囊和脾脏指数（P<0.05）;显著提高42日龄肉杂鸡的ND抗体水平和T淋巴细胞转化率（P<0.05）,表明在日粮中添加天蚕素抗菌肽能提高817肉杂鸡的生长性能和免疫功能,在本试验条件下,适宜的添加量为200 mg/kg。  相似文献   

14.
为了了解鸡传染性贫血病病毒（CIAV）和J亚群禽白血病病毒（ALV-J）对AA肉种鸡鸡胚和1日龄雏鸡的感染情况,试验直接从山东省3个不同AA肉种鸡场的鸡胚和1日龄雏鸡的肝、脾、肾、胸腺、骨髓、法氏囊等组织（器官）提取DNA,进行了PCR扩增及PCR产物的克隆和序列测定。结果显示,被检的3个肉种鸡场的鸡胚和1日龄雏鸡体内均有这2种病毒核酸的检出,其中CIAV的阳性率是20.42%,ALV-J的阳性率是15.83%,二者共感染的阳性率是6.25%;在阳性检出率中弱雏〉死胚〉健康雏〉正常胚。病毒核酸在各感染组织（器官）的含量也有所差异,CIAV以脾的含量最多,ALV-J以肾的含量最多。对肝进行细菌分离鉴定时发现,3个鸡场还存在大肠杆菌、沙门氏菌和霉形体的混合感染。结果提示,在AA肉种鸡鸡胚和雏鸡体内存在CIAV、ALV-J的感染和二者的共感染以及继发性大肠杆菌、沙门氏菌和霉形体的混合感染。  相似文献   

15.
This experiment was conducted to investigate the effects of antimicrobial peptide on growth performance and immune function of 817 broiler hybrids. Nine hundred one-day-old 817 broiler hybrids were chosen and randomly divided into 5 groups with 6 replicates per group and 30 broilers per replicate.The broilers in control group were fed with basal diet, broilers in the antibiotic group were fed with basal diet supplemented 150 mg/kg aureomycin,and that in groups Ⅰ,Ⅱ and Ⅲ were fed with basal diet supplemented 100,200 and 300 mg/kg antimicrobial peptide,respectively. The experiment period was 42 days. The results showed that average daily gain of 817 broiler hybrids in groups Ⅱ and Ⅲ was significantly increased (P<0.05),meanwhile the F/G was significantly decreased at 1-21 days old (P<0.05).The immune organ indexes of thymus,bursa of Fabricius and spleen were significantly increased at 21 and 42 days old (P<0.05),and the antibody level against ND and T lymphocyte transformation rate of 42 days old 817 broiler hybrids were improved (P<0.05). It indicated that antimicrobial peptide could increased growth performance and immune function of 817 broiler hybrids as same with aureomycin,and appropriate dosage of antimicrobial peptide was 200 mg/kg in this experiment.  相似文献   

16.
谷氨酰胺对肉鸡免疫器官胚后发育的影响   总被引：1，自引：0，他引：1  
160只1日龄艾维茵肉仔鸡随机分成4组。分别饲喂添加0％、0．2％、0．4％和0．8％谷氨酰胺（Gln）饲粮28d。每周末取鸡24只，每组6只，颈静脉放血致死，取胸腺、法氏囊和脾脏，称重．制作切片．光镜观察，研究基础日粮中添加Gln对肉鸡免疫器官胚后发育的影响。结果显示：（1）添加0．8％Gln组1、4周龄和添加0．4％Gln组2、4周龄胸腺重量显著高于对照组（P〈0．05），添加0．8％Gln组2周龄极显著高于对照组（P〈0．01）；添加0．4％Gln组1、2、4周龄和添加0．8％Gln组4周龄法氏囊重量显著高于对照组（P〈0．05），添加0．8％Gln组1、2周龄极显著高于对照组（P〈0．01）；试验各组脾脏重量均高于对照组。添加0．8％Gln组4周龄显著高于对照组（P〈0．05）。（2）试验组胸腺小叶皮质增厚，淋巴细胞密集，髓质比例减小。胸腺小体减少、减小。试验组法氏囊皱襞内淋巴滤泡增多，皮质增厚．淋巴细胞增多。试验组脾脏脾小结增多、增大；动脉周围淋巴鞘增厚；椭球增多、增大．细胞排列疏松．鞘毛细血管管腔变大．内皮细胞增高。间隙增大。添加0．8％Gln对肉鸡免疫器官发育影响最为明显。结果表明，日粮中添加Gln对肉鸡中枢淋巴器官胚后发育的促进作用优于外周淋巴器官。并有延缓法氏囊退化作用。本试验从组织学角度证明Gln能够促进机体淋巴细胞的增殖分化及免疫器官的胚后发育，进而提高机体的免疫功能。  相似文献   

17.
Mallard ducklings were fed diets containing corn naturally contaminated with mixed aflatoxins, purified T-2 toxin, or no detectable mycotoxin in two trials. The aflatoxin level used was 12 ppb in the first trial and 33 ppb in the second. T-2 was added at 2 ppm in both trials. No pathology was associated with the aflatoxin used in this study, and T-2--induced lesions were described in a previous publication. The weights of primary (thymus and bursa of Fabricius) and secondary (spleen) lymphoid organs were significantly reduced in the T-2--treated birds. The total number of viable cells recovered from the thymus was significantly reduced in aflatoxin-treated birds. The numbers of viable cells recovered from thymus, bursa of Fabricius, and spleen were all significantly reduced after treatment with T-2. In each trial, significantly lower mitogenic responses were seen to pokeweed mitogen and concanavalin A in birds fed aflatoxin or T-2, representing reduction in both B-cell and T-cell mitogenesis. Birds fed aflatoxin also had significantly reduced Escherichia coli O55 lipopolysaccharide-induced mitogenic responses. These studies indicate that subacute oral exposure to aflatoxin caused a loss of normal lymphocyte reactivity in mallard ducklings. This finding supports the hypothesis that waterfowl that ingest even small quantities of mycotoxin-contaminated waste grain are likely to be more susceptible to bacterial or viral infections.  相似文献   

18.
纳豆芽胞杆菌对黄羽肉鸡生产性能和免疫功能的影响   总被引：1，自引：0，他引：1  
为评价纳豆芽胞杆菌对黄羽肉鸡生产性能及免疫功能的影响,将270只1日龄雏鸡随机分为6组,分别在日粮中添加不同剂量的纳豆芽胞杆菌,检测其对试验鸡增重、免疫器官指数和新城疫疫苗免疫后的抗体水平的影响.结果显示:添加不同剂量的纳豆芽胞杆菌对试验鸡增重具有不同程度的促进作用,尤以106 cfu/g、107 cfu/g 2个浓度组效果显著,28日龄试验组鸡平均体重分别比对照组提高7.54%和9.37%,并可以显著提高胸腺、脾脏和法氏囊器官指数,且显著提高试验雏鸡血清新城疫HI抗体应答,到35日龄试验结束时仍持续高于对照组.表明在饲料中添加106 cfu/g、107 cfu/g的纳豆芽胞杆菌能显著改善肉鸡生产性能,增强免疫反应.  相似文献   

19.
In this study, 94 clinically ill 11-28-day-old chicks belonging to eight broiler units from the Marmara region were investigated clinically for changes in hematocrit values and for the presence of chicken anemia virus (CAV) DNA. CAV DNA was detected by polymerase chain reaction in the thymus of 8.5% of the chicks. These chicks showed clinical signs of diarrhea, anorexia, depression, and growth retardation. The hematocrit values of these chicks were between 24% and 38%. At necropsy, hemorrhages were observed in the leg and pectoral muscles. Atrophy was noted in the thymus and in the bursa of Fabricius of positive chicks, and hemorrhages in the proventriculus of one positive chick were observed. This report describes the first detection of CAV DNA in chicks in Turkey.  相似文献   

20.
1.?The purpose of this study was to investigate the protective effect of α-lipoic acid (LA) on aflatoxin (AF) toxicosis in chicks.

2.?Groups of 10 Ross PM3 chicks were given, for 21 d, no AF (C), 60 mg/kg/bwt of α-lipoic acid (LA), 150 ppb of aflatoxin (AF1), 150 ppb of aflatoxin plus 60 mg/kg/bwt of α-lipoic acid (AF1 + LA), 300 ppb of aflatoxin (AF2), and 300 ppb of aflatoxin plus 60 mg/kg/bwt of α-lipoic acid (AF2 + LA). Before the animals were killed, blood samples were drawn for haematological analysis, and then tissue samples were collected for histopathological investigation. Immunohistochemical staining was performed against inducible nitric oxide synthase (iNOS) and nitrotyrosine on liver samples. Apoptotic cell death in liver was assessed by in situ TUNEL assay. The malondialdehyde (MDA) and reduced glutathione (GSH) concentrayions in liver and kidney were also determined.

3.?Hydropic degeneration and occasional necrosis, bile duct hyperplasia and periportal fibrosis were observed in the livers of AF-treated groups. The severity of these changes was reduced in LA-supplemented AF groups. Occasionally, thymic cortical atrophy, lymphoid depletion in spleen and bursa of Fabricius, and degeneration in the kidney tubule epitheliums were detected in AF groups. The severity of these degenerative changes was slightly reduced in LA supplemented groups.

4.?There was moderate to strong iNOS and nitrotyrosine immunoreactivity in the livers of AF groups, while decreased immunoreactivity was observed against both antibodies in the LA supplemented groups. Apoptotic cells were numerous in the AF groups, while greatly reduced in LA supplemented groups.

5.?In the liver and kidney of AF-treated groups given 300 ppb of aflatoxin, MDA concentrations were increased as GSH decreased, compared to the control group. LA supplementation of AF-treated birds improved the results compared to the AF only groups, however a statistical difference was observed only in liver tissues between AF2 + LA and AF2 groups. Haematological variables showed no differences among the groups.

6.?In conclusion, supplementation of feed with the antioxidant LA, might ameliorate the degenerative effects caused by aflatoxin due to lipid peroxidation.  相似文献   


设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号