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饲用β-葡聚糖酶体外评判体系的研究 总被引：7，自引：0，他引：7
用蒸馏水于4℃对浙江酶,Avizyme-1500酶制剂抽提12h,在不同温度和不同pH条件下对两种酶制剂中的β－葡聚糖酶活力进行测定,结果表明：两种酶制剂中的β－葡聚糖酶在50－60℃,pH5.0-7.0时有较高的活力。同时对两种酶制剂进行高温处理,结果表明：85℃保温10、20、30min。浙江酶制剂中的β－葡聚糖酶分别失活97．72％、98．55％和完全失活,Avizyme-1500酶制中的β－葡聚糖酶失活97．58％和完全失活。 相似文献
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试验目的是利用体外酶解法快速评定不同酶制剂在小麦型日粮中的应用效果。针对小麦的营养特性,实验选用酶制剂1#(E1)、酶制剂2#(E2)、木聚糖酶、欧蒂酶等4种不同酶制剂(均以木聚糖酶和β-葡聚糖酶为主),测定其木聚糖酶和β-葡聚糖酶的酶活,然后在体外环境下模拟猪胃肠道消化环境,并使不同酶制剂与小麦-浓缩料型日粮在其中充分反应,然后利用DNS比色法测定反应物中还原糖总量(实验以葡萄糖计)来评定酶制剂的应用效果。实验结果表明,木聚糖酶和β-葡聚糖酶含量相对较高的E1反而酶解反应效果不明显,几乎检不出还原糖;相反,4种酶制剂中木聚糖酶和β-葡聚糖酶含量相对较低的欧蒂酶的应用效果则最好。结果表明,检测时酶活高的酶制剂其体外应用效果并不一定好,这可能与酶制剂的耐酸能力有关,在一定条件下能为使用者提供简便的检测依据。由于实际应用中酶制剂的影响因素很多,这次实验结果还需通过饲养试验进一步验证。 相似文献
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β-葡聚糖酶活力及稳定性研究 总被引：21，自引：0，他引：21
从确认β-葡聚糖是大麦中的抗营养因子以来,以β-葡聚糖酶为主体的粗酶制剂被用来提高大麦的营养价值.酶制剂中β-葡聚精酶活力高低,以及耐热、耐酸、耐保存、耐蛋白酶分解等特性是衡量这一类酶制剂质量的重要指标.β-葡聚糖酶主要由细菌或霉菌产生,各厂家使用不同菌株及不同的生产加工技术,使酶制剂的质量差别很大.本试验对不同来源酶制剂的β-葡聚糖 相似文献
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麦类饲料中粘性多糖的抗营养性及多糖酶的应用 总被引：2，自引：0，他引：2
麦类饲料电含有抗营养因子——粘性多糖(阿拉伯木聚糖和β-葡聚糖),限制了该类饲料的使用量。添加酶制剂可提高这些饲料的利用率。本文综述了麦类饲料中粘性多糖的含量、抗营养特性,酶制剂的应用及作用机理。 相似文献
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谷物饲料中的主要抗营养因子 总被引：14，自引：0，他引：14
早在１９５２年ＰｒｅｅｃｅＩ．Ａ和ＭａｃｒｅｎｚｉｅＫ．Ｇ．就已证实谷物饲料中主要含有两类粘性的非淀粉多糖物质：木聚糖和β－葡聚糖。木聚糖是一类由Ｄ－木糖单位主干（以β－１,４键相连）和Ｌ－阿拉伯糖分枝（ａ－１,２和ａ－１,３相连）所组成的聚合物,由于它是由阿拉伯糖和木糖两种单糖组成,故也叫阿拉伯木聚糖或戊聚糖。β－葡聚糖是一类由右旋葡萄糖以β－构型相连的同聚物,它有许多类型,谷物饲料中的β－葡聚糖是指葡萄糖以β－－１,３和β－１,４构型相连的聚合物。由于这两类多聚糖具有增加食糜粘度、降低采食量… 相似文献
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β-葡聚糖酶与木聚糖酶在猪饲料中的应用 总被引：3，自引：0，他引：3
在猪饲料中添加复合酶制剂可以降解大麦和燕麦中的 β-葡聚糖以及黑麦和小麦中的可溶性阿拉伯木聚糖。但酶制剂的添加对于猪的饲料转化率仅有很小的提高 ,这可能是由于饲料内源酶和小肠微生物的作用 ,大量的 β-葡聚糖和阿拉伯木聚糖在回肠前后已经降解 ,因此酶制剂在猪饲料中的应用效果不如鸡的应用效果好。1 ﹀-葡聚糖与阿拉伯木聚糖的结构β-葡聚糖是来源于大麦和燕麦糊粉层和胚乳层的一种部分可溶性的细胞壁多糖。这是由 β- 1 ,4糖苷键和 β- 1 ,3糖苷键组成 ,其中 β- 1 ,4糖苷键为主键。由于 β- 1 ,3糖苷键的存在 ,使 β-葡聚糖不… 相似文献
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随着养鸡业的发展和竞争的加剧，对饲料品质的要求也越来越高。由于鸡的消化道短，排空速度较快，以及饲料原料中含有某些抗营养因子，所以酶制剂越来越多地应用在饲料行业中。本试验验证了酶制剂在肉鸡日粮中的应用效果。
1 材料与方法
收稿日期：2000-04-06
1.1 酶制剂试验选用浙江大学正和动物保健饲料公司生产的利高酶，主要含有本聚糖酶、β-葡聚糖酶、蛋白酶、淀粉酶和纤维素酶。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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近几年,世界饲用添加剂市场增长较快,预计今后将进一步增长,据世界粮农组织(FAO)首领会议的倡议,到2015年,将使世界营养不良的人口削减一半,其间,肉制品将会以每年2％的速度增长,特别是加快猪肉和禽肉增长。这将促进世界添加剂,尤其是蛋氨酸和赖氨酸需求量的增加。 相似文献
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