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1.
为探究饲料中添加苹果渣对育肥猪生长性能及经济效益的影响,选择品种、胎次相近、平均体重为(90.57±1.01)kg的杜-长-大(Duroc×Landrace×large white)三元杂交猪18栏,按完全随机设计法分为3组,每组6个重复,每个重复5头猪,周期35 d。对照组饲喂基础日粮,试验A组饲喂基础日粮+2.5%苹果渣(等量替代麸皮),试验B组饲喂基础日粮+5.0%苹果渣(等量替代麸皮)。结果表明:与对照组相比,试验A组的日增重数据与之相近,采食量、料肉比略有所增加(P 0.05);试验B组的日增重略微降低,采食量、料肉比有所增加(P 0.05),且随着添加量的增加,日增重有逐渐降低的趋势,采食量、料肉比有逐渐增加的趋势;试验A组与B组的头均增重均较低,单位增重成本略高。因此,育肥猪饲料中用苹果渣替代2.5%、5.0%的麸皮是可行的,且不影响饲料的适口性,能降低头均增重。但目前苹果渣的价格较高,直接替代麸皮使用经济效益一般,可在价格较低时选择添加使用。  相似文献   

2.
试验旨在研究饲粮中添加不同含量的高粱和DDGS(谷物干酒精糟)与纯玉米-豆粕型饲粮对生长猪生长性能的影响。试验选取244头56 kg左右的杜长大三元杂种生长猪,随机分成3个处理,对照组饲喂玉米-豆粕型饲粮,试验1组饲喂15%DDGS+10%高粱饲粮,试验2组饲喂10%DDGS+20%高粱饲粮。结果表明,试验1组头均日采食量和头均日增重分别比对照组降低13.57%(P0.05)、8.00%(P0.05),料重比降低0.17,单位增重饲料成本减少0.71元/kg;试验2组的头均日采食量和头均日增重分别比对照组降低0.71%(P0.05)和4.00%(P0.05),料重比增加0.10,单位增重饲料成本增加0.09元/kg。  相似文献   

3.
小肽添加剂对生长猪生长性能的影响   总被引：2，自引：0，他引：2  
试验研究三种不同的小肽添加剂制品对生长猪生长性能的影响。试验选用72头初始体重在(34.64±8.32)kg的杜×长×大三元阉公猪,随机分为4个处理,每个处理6个重复,每个重复3头猪试验各组分别饲喂基础日粮(对照组),基础日粮加100mg/kg小肽A(小肽A组),基础日粮加100mg/kg小肽B(小肽B组),基础日粮加50mg/kg小肽C(小肽C组),试验期28d。测定其对生长猪的生长性能的影响,结果表明,与对照组相比,小肽A组、小肽B组和小肽C组的生长猪的平均采食量分别提高了9.8%、7.0%和12.5%(P0.05),平均日增重分别提高了15.8%、12.8%和21.7%(P0.05),料肉比分别降低了5.1%、6.3%和8.5%(P0.05)。  相似文献   

4.
本试验旨在研究发酵麸皮等量替代基础日粮中5%麸皮对育肥猪生长性能、屠宰性能及肉品质的影响。试验选择90 kg左右的杜×长×大三元杂交育肥猪,随机分为2组,每组6个重复,每个重复1头猪。对照组饲喂基础日粮,试验组将基础日粮中5%麸皮用发酵麸皮替代;预试期7 d,正式试验期26 d。结果表明:与对照组相比,试验组平均日增重显著增加13.49%(P0.05),平均日采食量无显著差异(P0.05),耗料增重比显著降低11.73%(P0.05);与对照组相比,试验组活体瘦肉率1、活体瘦肉率2均无显著差异(P0.05);试验组屠宰率、第10肋眼肌面积和第10肋背膘厚等屠宰性能指标均差异不显著(P0.05);与对照组相比,试验组48 h滴水损失降低18.84%(P0.05),肌内脂肪提高30.4%(P0.05)。综上分析,发酵麸皮等量替代基础日粮中5%麸皮可以有效提高育肥猪生长性能,改善肉品质。  相似文献   

5.
选择日龄相同、体重相近的保育猪90头、育成猪78头、育肥猪36头,分别随机分为3组,A组(对照组)饲喂基础日粮,B组、C组分别在基础日粮上添加0.05%、0.1%的复合酶制剂,探讨植物提取复合酶对生长猪生产性能的影响。结果表明,与对照组相比,保育猪B组、C组的平均日增重分别提高11.6%(P0.05)、14.0%(P0.05),平均采食量分别提高4.0%(P0.05)、6.7%(P0.05),料重比分别降低6.8%(P0.05)、6.3%(P0.05);育成猪B组、C组的平均日增重分别提高7.56%(P0.05)、2.87%(P0.05),平均采食量分别提高2.50%(P0.05)、2.70%(P0.05),料重比分别降低4.72%(P0.05)、0.17%(P0.05);育肥猪B组、C组的平均日增重分别提高18.83%(P0.01)、31.79%(P0.01),平均采食量分别提高6.67%(P0.01)、12.78%(P0.01),料重比分别降低10.24%(P0.05)、14.53%(P0.05);各试验组经济效益增加明显,腹泻率降低不显著。可见,日粮中添加植物提取复合酶可在一定程度上改善生长猪生产性能,提高养猪经济效益,其中保育猪、育肥猪添加0.1%优于0.05%,育成猪添加0.05%优于0.1%。  相似文献   

6.
本试验旨在研究日粮中添加不同比例棕榈粕对生长猪生长性能的影响。试验选取健康状况良好,胎次相近,平均体重(32.13依2.13)kg的杜长大三元杂交商品猪120头,随机分为4组,每组5个重复,每个重复6头猪。对照组饲喂基础日粮,试验组分别饲喂4%、7%、10%棕榈粕日粮。各组环境条件一致,自由采食和饮水,按照常规饲养管理进行,预饲期7天,试验期26天。结果显示院随着棕榈粕添加量(<7%)的增加,生长猪平均日采食量和平均日增重逐渐提高。7%棕榈粕组平均日采食量显著高于对照组(P<0.05),而4%棕榈粕组与对照组差异不显著(P>0.05);与对照组相比,4%棕榈粕组和7%棕榈粕组平均日增重均有升高的趋势但无明显差异(P>0.05)。当棕榈粕添加量达到10%时,平均日采食量和平均日增重比7%棕榈粕组显著降低,基本与对照组持平,且10%棕榈粕组料重比显著高于对照组(P<0.05)。因此,在生长猪(30kg)日粮中添加4%-7%棕榈粕可促进猪只采食量和生长性能,而当棕榈粕添加量达到10%时,会影响生长猪的采食量和生长性能。  相似文献   

7.
试验旨在探讨日粮中添加不同体外胃蛋白酶消化率的蛋白肽（A80、A90）对生长猪生长性能及养分表观消化率的影响。采用单因子随机设计,试验1：选取300头杜洛克猪×长白猪×大白猪三元生长猪,随机分为5组,每组4个重复,每个重复15头。Ⅰ组饲喂基础日粮,Ⅱ~Ⅴ组分别在基础日粮中添加1%、2%、3%、4% A80,试验期30 d。试验2：选取240头杜洛克猪×长白猪×大白猪三元生长猪,随机分为4组,每个组4个重复,每个重复15头。Ⅰ组饲喂基础日粮,Ⅱ~Ⅳ组分别在基础日粮中添加1%、2%、3% A90,试验期30 d。结果显示,试验1：各组间平均日增重、平均日采食量及料重比均无差异显著（P>0.05）,但随A80添加量的增加平均日增重有降低的趋势;Ⅱ组干物质、粗蛋白质表观消化率显著高于Ⅰ、Ⅲ、Ⅴ组（P<0.05）,但与Ⅳ组差异不显著（P>0.05）,随着A80添加量的增多,钙、磷表观消化率有所提高,但没有规律性。试验2：随着日粮中A90添加量的增加,平均日增重先增加后降低。Ⅲ组平均日增重显著高于Ⅰ组（P<0.05）,与其他组间差异不显著（P>0.05）,Ⅲ组平均日采食量显著高于其他组（P<0.05）。干物质、粗蛋白质表观消化率随着A90添加量的增加有下降的趋势。综上所述,在生长猪日粮中添加A80蛋白肽对生长性能没有改善作用,营养物质消化率均先增高后降低;随着A90蛋白肽添加量的增多,生长猪的生长性能呈二次性提高,最适宜剂量为2%~3%。  相似文献   

8.
《养猪》2017,(5)
试验旨在研究木薯渣不同添加比例对生长猪生长性能(日采食量、日增重、料重比)和经济效益的影响。试验选择250头体重35 kg左右的健康杜长大三元杂种肉猪,随机分为A、B、C、D、E 5组,每组5个重复,每个重复10头猪(公、母各半)。按等能等氨基酸配制饲粮,对照组(A组)饲喂基础饲粮,其余4组(B、C、D、E)分别在对照饲粮的基础上添加木薯渣4%、6%、8%、10%,试验期26 d。试验结果表明,1)生长性能:随着饲粮中木薯渣添加比例的增加,肉猪平均日采食量试验组与对照组无明显差异(P0.05)。日增重随着木薯渣添加比例的增加而升高,当木薯渣添加量为8%时达最高日增重,随着木薯渣添加量的继续增加日增重降低,木薯渣10%组日增重低于8%组。料重比随木薯渣添加比例的增加逐渐降低,当木薯渣添加量为8%时显著低于对照组(P0.05)。2)经济效益:肉猪每千克增重饲料成本,试验D组最低(4.28元),对照组A最高(4.54元),且试验组E与对照组A接近。综上所述,该试验条件下,35~70 kg生长猪饲粮中木薯渣的添加量以8%为宜。  相似文献   

9.
试验主要研究益生菌和酸性蛋白酶对生长肥育猪生产性能的影响。选杜×长×大生长肥育猪72头,随机分4组,试验期间,日粮不添加任何抗生素和化学合成药物。Ⅰ组为对照组,饲喂基础日粮;Ⅱ组添加0.15%的益生菌,Ⅲ组添加0.02%酸性蛋白酶,Ⅳ组添加0.15%益生菌和0.02%酸性蛋白酶。结果表明,与对照组相比,试验Ⅱ、Ⅲ、Ⅳ组分别提高了生长肥育猪平均日增重4.8%(P0.05)、8.3%(P0.05)、12.3%(P0.05),平均日采食量3.00%(P0.05)、5.76%(P0.05)、8.01%(P0.05);分别降低了生长肥育猪料重比1.8%、2.3%、3.8%,发病率62.6%、50%、100%;降低了增重成本1.34%、2.00%、3.05%。  相似文献   

10.
研究旨在针对目前中草药在三元杂交猪生长育肥研究的薄弱环节,探索中草药添加剂对生猪育肥的影响,为推广利用新型安全有效的植物性添加剂提供理论支持。选用日龄相近、60kg左右的三元杂交(杜×长×大)猪45头,随机分为A、B、C 3个组,每组15头。A组为对照组,B组、C组分别在日粮中添加0.5%和1%的中草药添加剂,研究中草药添加剂对生长育肥猪生产性能的影响。试验结果表明：B、C组的料重比、平均日耗料量显著低于A组(P＜0.05),但平均日增重显著高于A组(P＜0.05)。研究发现,在育肥猪日粮中添加中草药添加剂可以显著提高平均日增重,降低耗料量和料重比,且以添加量为1%时效果最好。  相似文献   

11.
Capromorelin is a ghrelin receptor agonist that is FDA approved for appetite stimulation in dogs. The objective of this study was to evaluate the safety of daily oral administration of capromorelin to cats over a range of doses and for an extended period. Two randomized, controlled studies were conducted: in Study 1, cats (n = 6 per group) received placebo or capromorelin at a dose of 9, 15, 30 or 60 mg/kg once daily for 14 days; and in Study 2, cats received capromorelin at 6 mg/kg (n = 8) or placebo (n = 4) once daily for 91 days. Cats were evaluated using clinical observations and clinical pathology test results for both studies, with the addition of postmortem examination in Study 1 and measurements of growth hormone and insulin‐like growth factor 1 in Study 2. Abnormal clinical observations were limited to emesis, hypersalivation, lethargy/depression, head shaking and lip smacking, which occurred more frequently in the capromorelin‐treated groups than in the placebo group. There were no clinically relevant differences in clinical pathology test results between the capromorelin and placebo groups in either study.  相似文献   

12.
对10个青贮玉米品种自然发病情况进行调查,并采用病级分类方法对抗病性分类。结果表明:对锈病表现抗性和中抗的品种有3个和5个,分别占供试品种的30%和50%;对大斑病表现抗性和中抗的品种有4个和5个,分别占供试品种的40%和50%;对褐斑病表现抗性和中抗的品种有6个和3个,分别占供试品种的60%和30%;对小斑病表现抗性和中抗的品种有6个和3个,分别占供试品种的60%和30%。多数品种对青贮玉米4种病害抗性表现较好,可从青贮玉米供试材料中选用优良抗病材料用于四川地区种植。  相似文献   

13.
Activation of polymorphonuclear cells (PMNs) leads to the formation of superoxide, which is in turn dismutated to H2O2 by superoxide dismutase (SOD) and is partly responsible for oxygen-dependent microbicidal activity. However, no comparative information is available on the effect of SOD inhibition before PMN activation to allow simulation of the SOD defects that are known to occur in some ruminants. This paper attempts to examine the degranulative and phagocytic responses in buffalo, cattle and goat PMNs exposed to diethyldithiocarbamate, a known SOD inhibitor. The activity of glutathione peroxidase and reductase was increased in the presence of SOD inhibitor. On activation, H2O2 production increased significantly (p<0.01), while SOD inhibition before the activation of PMNs caused a significant decline in the production of H2O2 (p<0.05) in all the species studied. There was a significant increase (p<0.05) in the phagocytosis of Candida albicans spores by buffalo PMNs activated with opsonized zymosan. Activation of bovine PMNs after exposure to the SOD inhibitor resulted in a significant decline (p<0.05) in phagocytic activity; in the other species, the two values only approached significance. Among the activators, opsonized zymosan caused a significant increase in phagocytic activity as compared to lipopolysaccharide, particularly in the PMNs of buffaloes (p<0.05). Increased fungicidal activity (p<0.05) occurred with opsonized zymosan-activated PMNs of all the species studied. The fungicidal activity was found to decline in PMNs exposed to SOD inhibitor before activation (p<0.05). Interestingly, the phagocytic activity of caprine PMNs was found to be lower than that of PMNs from cattle (p<0.05).  相似文献   

14.
This study was conducted to investigate the effect of different dietary ratios of 13 C to 12 C or 15 N to 14 N on their relative incorporation into tissues. Eighty male rats were used in two 21-day feeding trials in which they were fed diets with either high δ13C levels (δ13C = −13.89‰ and δ15N = 2.37‰ in experiment 1 and δ13C = −19.34‰ and δ15N = 4.73‰ in experiment 2) or low δ13C levels (δ13C = −17.90‰ and δ15N = 3.08‰ in experiment 1 and δ13C = −21.76‰ and δ15N = 0.53‰ in experiment 2), meanwhile, the dietary δ15N levels were designed to two ranks. Blood, liver, adipose and muscle tissues were collected on day 0, 3, 7, 14, and 21 for determination of 13 C, 12 C, 15 N and 14 N isotopes. Rat growth rate, antioxidant capacity and metabolic parameters were also assessed. The results indicate that adipose tissue tend to deplete 13 C before the stable isotopic ratios achieved final equilibrium. Therefore, feeds with different isotopic signatures had different incorporation rates into tissues. Low dietary 13 C levels decreased tissue δ13C values whereas high dietary 13 C levels did not alter tissue δ13C values during the 21-d experiment. Blood δ15N values were a reliable parameter in assessing the relative contribution of dietary nitrogen to tissues. This study revealed a relationship between dietary isotopic signatures and their incorporation rates into rat tissues. However, more studies are needed to illustrate the mechanism through which dietary isotopic ratios influence the extent of isotopic incorporation into the tissues.  相似文献   

15.

Objective

To test the postexposure analgesic efficacy of low doses of eugenol in zebrafish.

Study design

Prospective experimental study.

Animals

A total of 76 large adult zebrafish (Danio rerio).

Methods

Fish swimming behavior (median velocity, freeze time, high-speed swimming and distance moved in the vertical direction) was recorded in a 1.6 L video arena before and after exposure to eugenol (0, 1, 2, 5, 10 and 20 mg L?1). In a second experiment, fish were anesthetized with 2-phenoxy-ethanol and treated with an injection of 5% acetic acid (noxious stimulus), and then exposed to 0, 1, 2 and 5 mg L?1 eugenol. The fish swimming behavior was also recorded.

Results

The higher doses (10 and 20 mg L?1) reduced the median velocity, high-speed swimming and distance moved in the vertical direction, and increased the freeze time. Zebrafish behavior was not altered by eugenol (1, 2 and 5 mg L?1) after noxious stimulation.

Conclusions and clinical relevance

The change in the behavior of zebrafish associated with a noxious stimulus can be monitored and is a good model for studying analgesia in fish. Eugenol (10 and 20 mg L?1) induced zebrafish sedation. The response after a noxious stimulus was not affected by postexposure to lower doses, and thus we cannot recommend its use as an analgesic.  相似文献   

16.
本文以千日红组培苗为试验材料,研究培养基中氮形态及含量对千日红试管苗生长和开花诱导的影响。结果表明,1)相对于铵态氮(NH4+),硝态氮(No3-)作为唯一氮源更有利于千日红试管苗生长和开花诱导,但千日红在NH4+和NO3-同时存在的培养基中表现最佳。2)在20 mmol/LNH4+(NO3-)和5 mg/L PP333存在的条件下,试管苗生长基本随着培养基中NO3-(NH4+)含量的增加而增加,并在含40 mmol/L NO3-+20 mmol/L NH4+(即MS培养基中氮含量)的培养基中株高达到最大值5.91 cm;而叶片数和开花率则随着培养基中NH4+和NO3-含量的增加呈现先增加后下降的趋势,并在20 mmol/L NO3-+5 mmol/LNH4+培养基中达到最大值,分别为10.7片/株和38.89%。3)氮含量及形态配比结果表明,千日红试管苗开花率在培养基中氮总量为5 mmol/L、NO3-/NH4+为4/1时达到最大值39.95%,而株高和叶片数在氮总量为35 mmol/L,NO3-/NH4+为4/1时达到最大值8.52 cm和13.38片/株。千日红试管苗开花率与培养基中NO3-/NH4+显著正相关,而与氮总量及株高之间显著负相关。此外,培养基中氮含量及形态配比还显著影响无菌苗根系生长。  相似文献   

17.
The pharmacokinetic properties of three formulations of vitacoxib were investigated in horses. To describe plasma concentrations and characterize the pharmacokinetics, 6 healthy adult Chinese Mongolian horses were administered a single dose of 0.1 mg/kg bodyweight intravenous (i.v.), oral paste, or oral tablet vitacoxib in a 3-way, randomized, parallel design. Blood samples were collected prior to and at various times up to 72 hr postadministration. Plasma vitacoxib concentrations were quantified using UPLC-MS/MS, and pharmacokinetic parameters were calculated using noncompartmental analysis. No complications resulting from the vitacoxib administration were noted on subsequent administrations, and all procedures were tolerated well by the horses throughout the study. The elimination half-life (T1/2λz) was 4.24 ± 1.98 hr (i.v.), 8.77 ± 0.91 hr (oral paste), and 8.12 ± 4.24 hr (oral tablet), respectively. Maximum plasma concentration (Cmax) was 28.61 ± 9.29 ng/ml (oral paste) and 19.64 ± 9.26 ng/ml (oral tablet), respectively. Area under the concentration-versus-time curve (AUClast) was 336 ± 229 ng hr/ml (i.v.), 221 ± 94 ng hr/ml (oral paste), and 203 ± 139 ng hr/ml, respectively. The results showed statistically significant differences between the 2 oral vitacoxib groups in Tmax value. T1/2λz (hr), AUClast (ng hr/ml), and MRT (hr) were significantly different between i.v. and oral groups. The longer half-life observed following oral administration was consistent with the flip-flop phenomenon.  相似文献   

18.
The current NRC dietary selenium (Se) requirement (0.15 mg/kg) of broilers is primarily based on growth performance data reported in 1986. Our study aimed to determine optimal dietary Se levels of broilers fed a practical corn-soybean meal diet for the full expression of selenoproteins in various tissues. A total of 384 one-d-old male broilers (n = 8 replicates/diet) were fed a basal corn-soybean meal diet or the basal diet supplemented with 0.1, 0.2, 0.3, 0.4 or 0.5 mg Se/kg in the form of Na2SeO3 for 21 d. Regression analysis was conducted to evaluate the optimal dietary Se levels using broken-line, quadratic or asymptotic models. The activity of glutathione peroxidase (GPX) in the plasma, liver, kidney and pancreas, iodothyronine deiodinase (DIO) in the plasma, liver and pancreas, and thioredoxin reductase (Txnrd) in the liver and pancreas, the mRNA levels of Gpx1, Gpx4, Dio1, selenoprotein (Seleno) h, Selenop and Selenou in the liver, Gpx4, Dio1, Txnrd1, Txnrd2, Selenoh, Selenop and Selenou in the kidney, and Gpx1, Gpx4, Selenoh and Selenou in the pancreas, and the protein levels of GPX4 in the liver and kidney of broilers were influenced (P < 0.05) by added Se levels, and increased quadratically (P < 0.05) with the increase of added Se levels. The estimates of optimal dietary Se levels were 0.07 to 0.36 mg/kg based on the fitted broken-line, quadratic or asymptotic models (P < 0.001) of the aforementioned selenoprotein expression in the plasma, liver and kidney, and 0.09 to 0.46 mg/kg based on the fitted broken-line models (P < 0.001) of the aforementioned selenoprotein expression in the pancreas. The results indicate that the optimal dietary Se levels would be 0.36 mg/kg to support the full expression of selenoproteins in the plasma, liver and kidney, and 0.46 mg/kg to support the full expression of selenoproteins in the pancreas of broilers fed a practical corn-soybean meal diet from 1 to 21 d of age.  相似文献   

19.
An experiment was designed to determine the influence of fibre and betaine on the development of the intestine, liver and pancreas of broilers from hatch to 14 d of age. A total of 250-day-old Cobb 500 male broilers were allocated to 16 cages with 15 broilers each. Treatments were arranged in a 2 × 4 factorial design, consisting of 2 feed formulations (low and high fibre diets) and 4 levels of betaine (0, 1, 3 or 5 kg/t). At hatch, 10 birds in total were euthanised, and samples of the liver, pancreas, yolk sac and intestine were collected for reference of the analysed parameters before the start of the trial. On d 4, 9 and 14, 5 birds per cage (10 birds per treatment) were selected, euthanised and treated as the same as the birds at hatch. Villus height and width and crypt depth were determined on the duodenum samples, and absorptive area was calculated. The number of enterocytes in mitosis at the villus was determined by a positive reaction to antibody for Ki67 protein, and fused villus was evaluated visually. The relative weight of the yolk sac reduced (P < 0.05) as birds aged while the intestine and liver reached a maximum (P < 0.05) at around d 4 and the pancreas at d 9. Birds fed the high fibre diet had greater feed intake, lower relative weight of the pancreas and higher villus (P < 0.05) than birds fed the low fibre diet. Villus width increased (P < 0.05) at 4 d of age, and this was associated with fused villus. Betaine inclusion reduced (P < 0.05) villus width, increased (P < 0.05) villus size and absorptive area, and reduced (P < 0.05) the number of enterocytes with positive reaction for the antibody Ki-67. Betaine inclusion reduced the width and increased the absorptive area and the villus height of the duodenum of birds up to 14 d of age. The higher fibre diet increased feed intake and villus height, yet reduced pancreas relative weight, while not affecting body weight gain. This response was possibly due to a dilution effect of the fibre, reducing nutrient absorption and consequently stimulating villus growth to improve absorption rates.  相似文献   

20.
We report the cryopreservation of oocytes from Ban miniature pigs which are endemic in Vietnam. Immature cumulus‐oocyte complexes were collected from antral follicles of 7–8 mo old female cyclic Ban pigs and vitrified in micro‐drops. Oocyte morphology, lipid content, post‐warming survival, nuclear maturation, and embryo development were compared to those of oocytes from commercially slaughtered Landrace × Large white hybrid pigs. The size of oocytes in the two breeds was similar. However, significantly lower amounts of intracellular lipid were detected in Ban oocytes. There was no difference (p > 0.05) between Ban and Landrace × Large white oocytes in percentages of post‐warming survival (93.1 ± 3.4% vs. 70.7 ± 16.7%, respectively) and nuclear maturation after in vitro maturation (80.4 ± 5.1% vs. 90.0 ± 1.3% respectively). Similarly, cleavage (30.8 ± 7.8% vs. 10.3 ± 6.1%, respectively) and blastocyst development rates (9.4 ± 5.0% vs. 0.79 ± 0.79, respectively) were not different (p > 0.05) between vitrified Ban and Landrace × Large white oocytes after in vitro fertilization and embryo culture. In conclusion, high survival and maturation rates were achieved after vitrification of immature Ban oocytes and their cryo‐tolerance was similar to that of Landrace × Large white oocytes, despite the difference in lipid content. We succeeded to generate reasonable rates of blastocysts from vitrified Ban oocytes by in vitro fertilization.  相似文献   

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