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1.
杜光波 《当代畜牧》2006,(10):14-16
在淮安的4个地区,按不同季节,采集32份患有子宫内膜炎的奶牛子宫分泌物,进行细菌学分离鉴定与统计分析。结果共分离出84株细菌,其中金黄色葡萄球菌17株,占20.2%;表皮葡萄球菌9株,占10.7%;粪链球菌2株,占2.3%;化脓链球菌7株,占8.3%;大肠杆菌14株,占16.7%;变形杆菌7株,占8.3%;克雷伯氏菌7株,占8.3%;志贺氏菌3株,占3.6%;沙门氏菌7株,占8.3%;绿脓杆菌5株,占6%;白色念珠菌2株,占2.3%;化脓放线菌4株,占4.7%。经分析发现,奶牛子宫内膜炎病原菌的种类季节差异明显,夏秋以球菌感染为主,冬春季节以肠道杆菌感染为主。  相似文献   

2.
奶牛子宫内膜炎主要病原菌的调查   总被引:2,自引:2,他引:0  
为了掌握淮安地区奶牛子宫内膜炎主要病原菌的种类及分布情况,以便给淮安地区奶牛场制定科学的预防计划,在淮安的4个地区,按不同季节,采集32份患有子宫内膜炎的奶牛子宫分泌物,进行细菌学分离鉴定与统计分析。结果共分离出84株细菌。其中,金黄色葡萄球菌17株,占20.2%;表皮葡萄球菌9株,占10.7%;粪链球菌2株,占2.3%;化脓链球菌7株,占8.3%;大肠杆菌14株,占16.7%;变形杆菌7株,占8.3%;克雷伯氏菌7株,占8.3%;志贺氏菌3株,占3.6%;沙门氏菌7株,占8.3%;绿脓杆菌5株,占6%;白色念珠菌2株,占2.3%;化脓放线菌4株,占4.7%。经分析发现,奶牛子宫内膜炎病原菌的种类季节差异明显,夏秋以球菌感染为主,冬春季节以肠道杆菌感染为主。  相似文献   

3.
从40头患子宫内膜炎奶牛的子宫阴道分泌物中,经细菌的分离鉴定,共分离出52株细菌,其中链球菌18株、大肠杆菌13株、葡萄球菌10株、变形杆菌和克雷伯氏菌各3株、棒状杆菌2株、绿脓杆菌1株及其他细菌2株。  相似文献   

4.
广州地区奶牛子宫内膜炎病原菌检验   总被引:17,自引:0,他引:17  
从40头患子宫内膜炎奶牛的子宫阴道分泌物中,经细菌的分离鉴定,共分离出52株细菌,其中链球菌18株,大肠杆菌13株,葡萄球菌10株,变形杆菌和克雷伯氏菌各3株、棒状杆菌2株,绿脓杆菌1株及其它细菌2株。  相似文献   

5.
为探讨奶牛子宫内膜炎和隐性乳房炎之间的相关性,在宁夏地区3个大型奶牛场(根据临床型子宫内膜炎的诊断标准)选择子宫内膜炎患牛111头,用兰州隐性乳房炎检测法检测(LMT)乳样,其中82.5%的病牛同时患有隐性乳房炎,然后进行了子宫样和乳样的采集,并用常规细菌分离、培养结合高智能全自动细菌鉴定及药敏分析系统(VITEK 2 Campact),确定引起宁夏奶牛子宫内膜炎的病原菌有18种110株,引起乳房炎的病原菌有23种107株,其中大肠杆菌、停乳链球菌、克氏库克菌、金黄色葡萄球菌、假单胞杆菌、乳房链球菌、产色葡萄球菌、蜡样芽孢杆菌、荧光假单胞杆菌、产酸克雷伯菌、副溶血弧菌、克雷伯氏菌可以同时引起奶牛子宫内膜炎和隐性乳房炎。  相似文献   

6.
粪链球菌对奶牛子宫内膜炎致病菌的体外抑菌试验   总被引:9,自引:0,他引:9  
为了给应用微生态制剂防治奶牛子宫内膜炎提供依据,将从健康母猪生殖道中分离的一株粪链球菌,与从奶牛子宫内膜炎分离的6种致病菌(溶血性链球菌、埃希氏大肠杆菌、金黄色葡萄球菌、克雷伯氏菌、变形杆菌、绿脓杆菌)分别混合培养,观察了粪链球菌对这些致病菌的抑制作用。结果表明,粪链球菌与这些病原菌等量混合培养,或加大致病菌用量后混合培养,或粪链球菌先培养一定时间后再与致病菌混合培养,在混合培养一定时间后,各致病菌的生长都受到抑制。  相似文献   

7.
规模化猪场母猪子宫内膜炎致病菌的分离鉴定   总被引:4,自引:0,他引:4  
从福建省南平市6个种猪场采集52头猪子宫内膜炎病猪子宫分泌物,分别进行致病菌分离鉴定,从52例子宫内膜炎患病母猪的活体病料中共分离细菌72株,检出率为150%。全部细菌分属为大肠杆菌、链球菌、葡萄球菌、沙门氏菌、绿脓杆菌、化脓棒状杆菌和克雷伯氏菌,分离到的细菌以大肠杆菌、链球菌和葡萄球菌占较大比例,它们分别占总检出菌株的43.0%、23.6%和16.7%。患猪中有61.5%的母猪为单一细菌感染;其它为细菌混合感染,混合感染比例,达到38.5%,其中以大肠杆菌造成的混合感染占比较高的比例,占总感染头数的28.8%。  相似文献   

8.
根据对周边猪场调查发现,因子宫内膜炎原因占淘汰生产母猪的比例达35%~55%,对母猪子宫内膜炎的发病原因、临床表现及防治方法介绍如下。1病因分析病原微生物感染,如大肠杆菌、链球菌、绿脓杆菌、化脓棒状杆菌、沙门氏菌、葡萄球菌、克雷伯氏菌、变形杆菌、假单胞菌以及病原性真菌等病原微生  相似文献   

9.
为了掌握郑州市奶牛子宫内膜炎发病情况,为奶牛子宫内膜炎病的预防提供参考依据。方法:采集确诊为子宫内膜炎奶牛的子宫内容物样品进行子宫内膜炎细菌学分析,对郑州市88头奶牛子宫内膜炎样品进行细菌的分离和鉴定。结果感染的细菌主要有金黄色葡萄球菌、大肠埃希菌、产吲哚金黄杆菌、浅黄金色单胞菌、奥斯陆莫拉菌和蜡样芽孢杆菌等56个菌株,大多数为混合感染。表明郑州市奶牛子宫内膜炎感染普遍存在,葡萄球菌和大肠埃希菌是奶牛子宫内膜炎的主要致病菌。  相似文献   

10.
近年来,随着牛养殖规模的增加,混合感染引发的疾病较严重。广西某牛场送来一例奶牛急性死亡病例,通过临床观察、细菌分离鉴定、病原鉴定、致病性试验和药敏试验的方法进行诊断。检测结果发现,内脏有明显的病理变化,从腹股沟淋巴结和脾脏分别分离出两种菌,经测序鉴定为肺炎克雷伯氏菌和大肠杆菌,且对小白鼠的致死率分别为80%和100%。17种药物的药敏结果显示这两种菌只对阿米卡星、大观霉素等少数几种抗菌药物敏感。PCR或RT-PCR未检测出IBRV、BPIV3、牛支原体。结论确诊该病例为肺炎克雷伯氏菌和大肠杆菌混合感染。  相似文献   

11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。  相似文献   

12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.  相似文献   

13.
14.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

15.
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed.  相似文献   

16.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

17.
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.  相似文献   

18.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

19.
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors.  相似文献   

20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems.  相似文献   

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