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1.
为了解贵州省H7亚型禽流感感染情况,采用HI试验对全省9个地区家禽血清样本进行血清抗体检测,PT-PCR方法对家禽咽-肛拭子、养禽场环境样本和野鸟粪便进行病原核酸检测。结果显示:在1570份家禽血清样本中,H7亚型禽流感血清抗体阳性率为0.45%(7/1570);在350份家禽咽-肛拭子和310份养殖场环境样本中,均未检测出H7亚型禽流感病毒核酸;在160份野鸟粪便中,有1份为H7亚型禽流感病毒核酸阳性,阳性率为0.63%(1/160)。试验提示,在贵州省养禽场中不存在H7亚型禽流感感染,但迁徙野禽中可能存在H7亚型禽流感隐性感染,应当引起重视。 相似文献
2.
为了解云南省玉溪市活禽交易市场禽流感病毒污染情况,2021年对该市8个县（市、区）活禽市场进行采样检测。全年共采集63场次1 455份棉拭子样品（禽咽喉/泄殖腔双拭子860份、环境拭子317份、运输工具拭子278份）,采用荧光RT-PCR方法进行流感病毒A型、H5/H7/H9亚型流感病毒核酸检测。共检出A型流感病毒核酸阳性435份,未检测到H5和H7亚型病毒核酸阳性;除检出7份未分型阳性样品外,在44场次检出H9亚型病毒核酸阳性428份,场次阳性率为69.84%,样品阳性率为29.42%,其中活禽、环境、运输工具样品阳性率分别为32.56%、25.87%、23.74%,差异具有统计学意义（P＜0.05）。一年四季均能检测到H9亚型病毒阳性,但3—6月份样品阳性率低于年均阳性率,1、2、7、10、12月样品阳性率明显高于其他月份,各月份间样品阳性率差异具有统计学意义（P＜0.01）。各县（市、区）的场次阳性率存在差异（P＜0.05）,但样品阳性率差异无统计学意义（P＞0.05）。结果表明：玉溪市活禽市场H5、H7亚型高致病性禽流感病毒的污染状况得到有效控制,但H9亚型禽流感病毒污染面依然广,污染率较高;不同月份间阳性率差异明显,但季节性差异缩小。结果提示,应继续加强活禽市场H9亚型禽流感病毒污染监测,同时强化活禽市场的检疫监管,严格执行消毒和休市制度,防止活禽市场禽流感病毒传入养殖环节。 相似文献
3.
为了更好地了解2022年湘阴县家禽中禽流感抗体水平和活禽批发市场禽流感病毒污染情况，通过春、秋两季免疫效果评估以及每个季度对两个活禽交易市场进行禽流感病毒专项监测，运用血凝抑制试验与实时荧光定量PCR的方法对血清及拭子样本进行禽流感病毒检测。结果显示：1200份禽血清中H5亚型AIV抗体整体合格率达到76.5%,H7亚型AIV抗体整体合格率达到78.8%;320份禽拭子和64份环境拭子中，H5亚型AIV阳性7份，阳性率1.8%,H7亚型AIV阳性数8份，阳性率2.0%。结果表明，家禽交易市场长期处于禽流感病毒污染状态。建议加强活禽市场管理，严格落实“1110”制度，确保禽类及产品的安全性和质量，降低禽流感病毒的传播速度和缩小传播范围。 相似文献
4.
为了解华东地区某市活禽市场中禽流感病毒的污染状况,2014—2016年对该市活禽市场的家禽进行了禽流感流行病学调查。结果显示:该市活禽市场禽流感病毒污染面较广,94.12%的市场存在禽流感病毒污染;H9N2亚型病毒感染率较高,样品总体阳性率为22.66%,场点总阳性率为94.12%;H7N9亚型病毒感染率经历了一个升降过程,2015年上半年达到高峰,之后显著降低;农贸市场中的H7N9亚型病毒分离率显著高于批发市场。本次调查未在活禽市场中检出H5亚型高致病性禽流感病毒。这些数据为深入了解活禽市场中禽流感病毒的污染状况和该病防控措施的制定提供了技术支持。 相似文献
5.
为了解2015年广州市禽类市场的禽流感病毒污染情况,为科学制定人感染禽源流感病毒的防控措施提供基础数据,对2015年广州市12个区部分禽类市场的3 447份样本进行了禽流感病毒荧光RT-PCR检测。结果显示：全部样品的A型禽流感病毒和H9亚型禽流感病毒的阳性率分别为12.56%和6.24%。1月份为A型和H9亚型禽流感阳性率最高的月份,阳性率分别为23.94%和14.73%。从化区的A型禽流感病毒阳性率最高（24.48%）,黄埔区的H9亚型禽流感病毒阳性检出率最高（14.76%）。不同类型样品中,A型禽流感病毒阳性率最高为砧板、刀具类（15.16%）,最低为冰鲜生鲜（7.78%）。H9亚型禽流感病毒阳性检出率最高为笼具（8.25%）,最低为置物台面（2.84%）。结果表明：广州市禽类市场冬春季节禽流感病毒阳性率较高;全市各区禽类市场普遍存在禽流感病毒污染;禽类交易各环节中,宰杀和饲养环节的污染最为严重。建议在加强活禽交易市场全面清洗消毒的基础上,对不同季节、不同交易环节,采取差异化的和基于风险的消毒、监测措施,以降低人通过禽类市场感染禽流感病毒的风险。 相似文献
6.
为监测天门市活禽调运禽流感免疫情况和病毒的分布状况,对该市辖区2018年8月至12月调运送检的禽类养殖场样品,采用血凝抑制试验检测禽流感H7亚型、普通PCR技术检测禽流感通用型及H5、H7亚型,并进行统计学分析。结果共检测1830份血清和对应的禽拭子,样品来源于61个养殖场,禽流感H7亚型免疫阳性率按禽种类分类分别是种鹅100%、蛋鸡90.52%、蛋鸭33.00%;禽流感通用型及H5、H7亚型的病毒核酸阳性率分别为0.18%、0.00%、0.00%。表明该市活禽调运中,水禽禽流感H7N9的免疫抗体群体合格率低,有禽流感通用型病毒存在。加强水禽调运前免疫、运输车辆消毒及宣教工作是预防和控制H7N9禽流感在流通环节发生的有效手段。该结果为及时发现疫情隐患和防控薄弱环节提供了指导依据。 相似文献
7.
2017年1月11日至17日,江门地区活禽批发市场休市7d,我们通过对辖区内3个活禽批发市场休市前、中、后的禽流感病毒进行监测检测,结果显示,无论是哪种采样环境,还是休市前、中、后的样品,均未检出H7亚型流感病毒核酸,H5亚型也是极少数量,只在休市后的屠宰车间样品中检出1份H5亚型。活禽批发市场实行临时休市活禽零存栏和彻底清理消毒等措施可降低档口环境的禽流感病毒污染,是科学有效的,但是活禽批发市场休市前后的屠宰车间和活禽咽/泄殖腔样品中的A型流感、H9亚型阳性率无统计学差异,说明休市前后活禽屠宰环节也存在禽流感病毒污染与传播的风险,要进一步加强活禽屠宰和养殖等环节的防控措施。防控禽流感特别是防止人感染H7N9亚型流感单单在活禽市场环节采取措施仍不够,应综合多方落实措施,才能实现更好地防控。 相似文献
8.
为掌握玉溪市禽流感在养殖场和活禽市场流行情况，2022年采集玉溪市9个县(市、区)32个乡(镇)62个村90个养殖场(户)及21个活禽市场2 280份样品进行监测。采用荧光RT-PCR方法对A型禽流感病毒检测并进行H5/H7/H9亚型禽流感病毒核酸分型。2022年全市共检出A型禽流感病毒核酸阳性240份，样品阳性率为10.53%,其中H9亚型233份、不确定型7份，未检出H5/H7亚型。活禽市场A型禽流感病毒核酸阳性率(31.47%)显著高于养殖场阳性率(0.27%),差异具有统计学意义(P<0.01);秋冬季节样品阳性率较高，差异具有统计学意义(P<0.01)。结果：玉溪市禽流感病毒主要分布于活禽市场，且以H9亚型流行为主，污染面较广，不同季度阳性率差异明显，但季节性差异缩小。结果提示：活禽市场是当前禽流感防控的重点，继续加强活禽市场的监测，同时强化活禽市场的检疫监管，严格执行消毒和休市制度，防止活禽市场禽流感病毒进入养殖环节。 相似文献
9.
为了解当地活禽批发市场的禽流感病毒污染状况,提升禽流感防控针对性,2015—2016年先后4次对长沙市两大活禽批发市场进行了禽流感流行病学调查,共在35个摊位分离到禽流感病毒,发现群阳性率为43.6%,禽流感病毒总分离率为14.24%,分离到H3、H5、H6、H7、H9和H10等6种亚型禽流感病毒。其中,H9和H5亚型禽流感病原阳性率较高,分别为8.7%和3.8%。调查结果表明,长沙市活禽批发市场中的禽流感病毒污染面较广。建议加强活禽市场管理,特别要做好市场的清洗消毒工作。 相似文献
10.
自主活动鸟类禽流感及新城疫的生态学与流行病学调查分析 总被引：6，自引：1，他引：6
在2004年初H5亚型禽流感在中国内地引起暴发前后，于湖北、广西二疫区市县的生态系中，自健康的鹰、鸽子、野鸭和鹌鹑中采集了54份血清，应用血凝抑制试验（HI）对其进行了血清学检测，并将获得的血清学检测数据进行了统计学分析。分析结果显示，在总共采集的54份血清样品中，抗新城疫病毒（NDV）抗体、抗It6亚型禽流感病毒（H6）抗体、抗H9亚型禽流感病毒（H9）抗体、抗H5亚型禽流感病毒（H5）抗体阳性率分别为64．8％、64．8％、75．9％、29．6％。推断这一时期新城疫病毒（NDV）、H6亚型禽流感病毒（H6）、H9亚型禽流感病毒（H9）和H5亚型禽流感病毒（H5）共循环于该生态系中，其中新城疫病毒、H9、H6亚型禽流感病毒长期、稳定循环于该生态系中，为该生态系主要流行血清型。血凝抑制试验检测结果发现，抗H5亚型禽流感病毒抗体效价较低，最高仅为640，其阳性率为29．6％，推断其可能为新近传入的病毒或新近重组变异出现的新病毒、与该次流感暴发相关的新病毒，这一推断还有待于通过对病毒基因序列的分析而做进一步验证：另外，新城疫病毒在该生态系中的稳定循环，构成了对家禽持久、潜在的威胁。对于自1994年以来，长期稳定循环于我国生态系及家禽中的H9亚型禽流感病毒则仍然是以G9-like为优势亚组。本研究结果还提示我们，对我国自主活动鸟类的生态系中抗禽流感病毒抗体的监测是预警、预报我国家禽流感暴发的有效途径，也是十分必要的，同时，血清学的检测结果为我们进一步的分子水平的分析研究提供了明确的靶向。 相似文献
11.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1986,27(9):324-328
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).
Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.
相似文献12.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(6):504-508
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%). 相似文献
13.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1985,26(10):323-327
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.
Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.
相似文献14.
15.
Slocombe JO Villeneuve A 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(10):630-633
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium. 相似文献
16.
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.
Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.
相似文献17.
Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(8):641-646
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%). 相似文献
18.
Owen J Slocombe D McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1987,28(8):491-495
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).
Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.
相似文献19.
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Slocombe JO McMillan I 《The Canadian veterinary journal. La revue veterinaire canadienne》1984,25(9):347-350
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.
Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.
相似文献