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1.
应用抗ＩＢＤＶ独特型抗体分别与福氏完全佐剂和福氏不完全佐剂按１∶１比例乳化制备成抗ＩＢＤＶ独特型抗体疫苗，免疫接种ＳＰＦ鸡和普通京白公鸡，然后用ＩＢＤＶ野毒株经点眼和滴鼻方式攻毒，保护率分别为８／８，１４／１４，由此证实了抗独特型抗体疫苗潜在的研究价值和应用价值。 相似文献
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应用抗ＩＢＤＶ独特型抗体分别与福氏安全佐剂和福氏不完全佐剂按１：１比例乳化制备成抗ＩＢＤＶ独特型抗体疫苗，免疫接种ＳＰＦ鸡和普通京白公鸡，然后用ＩＢＤＶ野毒株经点眼和滴鼻方式攻毒，保护率分别为８／８，１４／１４，野毒株经点眼和滴鼻方式攻毒，保护率分别为８／８，１４／１４，由此证实了抗独特型抗体疫苗潜在研究价值和应用价值。 相似文献
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应用提取纯化的抗ＩＢＤＶ ＩｇＧ免疫Ｂａｌｂ／ｃ小鼠，其脾细胞与ＳＰ２／Ｏ细胞在ＰＥＧ作用下融合，应用ＥＬＩＳＡ法检测筛选，经有限稀释法克隆２次，获得了２株（２Ｂ６株、５Ｆ４株）分泌抗ＩＢＤＶ独特型抗体的杂交瘤细胞株，并能诱生Ｂａｌｂ／ｃ小鼠产生高效价的含抗ＩＢＤＶ独型抗体腹水。 相似文献
4.
以提纯鸡ＩｇＧ做抗原免疫Ｂａｌｌ／ｃ小鼠，取鼠细胞在ＰＥＧ１０００作用下与小鼠骨髓细胞（Ｓｐ２／ｏＡｇ１４）融合，采用间接免疫荧光（ＩＦＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）检测上清抗体，阳性孔经有限稀法进行细胞克隆，共获得了７株分泌抗鸡ＩｇＧ单克隆抗体的杂交瘤细胞（２Ｈ８、４Ｄ８、４Ｄ８、１Ｂ７、２Ａ７、２Ｂ３、１Ｇ１２、３Ｄ１２）将这些细胞分别接种间系小鼠制备出腹水抗体，ＥＬＩＳＡ效价可达１０＾ 相似文献
5.
兔源抗IBDV独特型抗体疫苗的制备 总被引：3，自引：0，他引：3
用纯化的抗鸡传染性腔上囊病病毒（ＩＢＤＶ）抗体免疫家兔，将所获效从较高（１：１６）的兔源抗ＩＢＤＶ独特型抗体分别与福氏完全和福氏不完全佐剂按１：１比例乳化制备成抗ＩＢＤＶ独特型抗体疫苗，用该疫轩免疫迪卡公雏鸡和ＳＰＦ组鸡，经用ＩＢＤＶ强毒株以点眼和滴鼻方式攻击，试验组的保护率分别为９８／９８，１００／１００，４９／４９，大群免疫接种的应答率为１００％。 相似文献
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7.
抗鸡传染性法氏囊病病毒单克隆抗体中和株的建立 总被引：6，自引：1，他引：5
将纯化的鸡传染性法氏囊病病毒（ＩＢＤＶ）组织毒免疫的ＢＡＬＢ／ｃ鼠脾细胞与ＳＰ２／０骨髓瘤细胞融合，用ＥＬＩＳＡ，病毒中和试验检测分泌抗体，获得了６株稳定分泌抗ＩＢＤＶ单隆抗体的杂交瘤细胞，命名为ＮＩＢ－１，ＮＩＢ－２，ＮＩＢ－３，ＮＩＢ－４，ＮＩＢ－５，ＮＩＢ－６；６株ＭＣＡｂ均具ＥＬＩＳＡ特性和免疫沉淀特性，亚类鉴定表明，前４株属于ＩｇＧ２ａ，后２株属于ＩｇＧ１。杂交瘤细胞冻存６个月后复苏，均 相似文献
8.
采用腹腔的接种１次脾内注射禽呼肠孤病毒（ＡＲＶ）蛋白免疫小鼠，经３次融合后，共筛选８株分泌抗禽呼肠孤病毒（单克隆抗体杂交瘤细胞株，这８株ＭｃＡｂ均可与ＡＲＶＳ１１３３株，ＦＤＯ株发生反应，而与ＩＢＤＶ，ＭＤＶ，ＥＤＳ－７６病毒不发生反应，经亚类鉴定，ＡＥ７，ＡＦ８，ＢＤ１，ＤＨ１０，ＥＥ５为ＩｇＧ１；ＡＤ６，ＣＧ４为ＩｇＣ２ａ，ＡＧ７为ＩｇＧ２ｂ。腹水效价在１０＾３～１０＾５之间。 相似文献
9.
抗奶牛衣原体单克隆抗体杂交瘤细胞株的建立 总被引：3，自引：1，他引：2
将奶牛衣原体抗原免疫的 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠脾细胞与 Ｓ Ｐ２／ Ｏ 细胞在聚乙二醇作用下融合, 用间接 Ｅ Ｌ Ｉ Ｓ Ａ 试验筛选, 以有限稀释法克隆３ 次, 得到６ 株分泌抗奶牛衣原体单克隆抗体（ Ｍｃ Ａｂ） , 选择抗体分泌较高的 Ｇ２ 、 Ｆ２３ 、 Ａ 株进行详细的研究, 结果表明, 其核内染色体数为９０３５ 、９２１４ 、９４４２ ; 抗体属性为 Ｉｇ Ｇ２ａ 、 Ｉｇ Ｇ１ 、 Ｉｇ Ｍ, 用交叉 Ｅ Ｌ Ｉ Ｓ Ａ 法对３ 株 Ｍｃ Ａｂ 作特异性分析, 该 Ｍｃ Ａｂ 只与奶牛衣原体抗原, 猪衣原体抗原、羊衣原体抗原发生反应,而不与沙眼衣原体抗原、伪狂犬病抗原、布鲁氏杆菌抗原发生反应。 相似文献
10.
马立克氏病病毒囊膜糖蛋白B抗原Ⅰ型特异性和群共同性决定簇的单克隆抗体 总被引：3，自引：1，他引：2
用能表达马立克氏病病毒（ＭＤＶ）糖蛋白Ｂ（ｇＢ）的重组杆状病毒感染的Ｓｆ９细胞免疫小鼠，制备针对ＭＤＶｇＢ的单克隆抗体。以Ⅰ型马立克氏病病毒ＧＡ株感染的鸡胚成纤维细胞作为检测抗原，同时以免疫荧光试验（ＩＦＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）来筛选杂交瘤细胞，结果获得了ＩＦＡ和ＥＬＩＳＡ均为阳性的２株单克隆抗体细胞株，定名为ＢＡ４和ＢＤ８。在ＩＦＡ和免疫沉淀试验中，单抗ＢＤ８与Ⅰ、Ⅱ、Ⅲ型ＭＤＶ均呈阳性反应；单抗ＢＡ４只对Ⅰ型ＭＤＶ（包括ＣＶＩ９８８疫苗株）呈阳性反应。免疫沉淀反应进一步确证２株单抗识别的是ＭＤＶ糖蛋白Ｂ抗原。 相似文献
11.
试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。 相似文献
12.
Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections
of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal
(GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April
2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs
located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming
areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology
laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum
columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying
degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal
abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy
season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different
nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those
observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes.
But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the
results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia. 相似文献
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Tropical Animal Health and Production - 相似文献
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Rassnick KM Gieger TL Williams LE Ruslander DM Northrup NC Kristal O Myers NC Moore AS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2001,15(3):196-199
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks. 相似文献
15.
H Hayashi T Yoshimura J Y Chen 《Comparative immunology, microbiology and infectious diseases》1985,8(2):73-87
Our particular attention in this article was given to natural mediators for macrophages isolated from the sites of tissue injury. A number of chemotactic factors, which may satisfy many criteria making them acceptable as inflammatory leucocyte chemotactic factors, has been separated. Among them, our laboratory has isolated three macrophage (monocyte) chemotactic factors (MCF-a, -b and -c). Their purification, characterization and functional specificity are discussed. 相似文献
16.
Davis MS Willard MD Nelson SL Mandsager RE McKiernan BS Mansell JK Lehenbauer TW 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(3):311-314
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large. 相似文献
17.
B. Cinar Kul N. Bilgen J.A. Lenstra O. Korkmaz Agaoglu B. Akyuz O. Ertugrul 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2015,132(6):449-453
Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded. 相似文献
18.
Furosemide continuous rate infusion in the horse: evaluation of enhanced efficacy and reduced side effects 总被引：1，自引：0，他引：1
Johansson AM Gardner SY Levine JF Papich MG LaFevers DH Fuquay LR Reagan VH Atkins CE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):887-895
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses. 相似文献
19.
Ling GV Thurmond MC Choi YK Franti CE Ruby AL Johnson DL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(6):817-823
The objective of this report was to characterize 20-year changes in proportion of calcium oxalate (CaOx) calculi and struvite calculi in dogs, and associations with breed, age, and sex. In this retrospective study, results of analysis of urinary calculi from dogs were reviewed for specimens received between July 1, 1981, and December 31, 2001. Breed, sex, age, year of submission of the specimen, and mineral type(s) were analyzed statistically. CaOx or Struvite or both were contained in 18,966 of 20,884 (91%) specimens. For both sexes, a 20-year statistically significant increase was observed in the proportion of calculus specimens that contained CaOx. The increase in this proportion was greater in females (1% to 31%) than in males (18% to 82%). From 1998 to 2001, when proportions may have plateaued, the odds of specimens containing CaOx were markedly higher in 18 breeds, markedly lower in 5 breeds, and not significantly different in 13 breeds compared with crossbreds. For both sexes, a 20-year statistically significant decrease was observed in the proportion of calculus specimens that contained struvite. This decrease in proportion was greater for males (79-16%) than for females (97-68%). From 1998 to 2001, when proportions plateaued, the odds of calculi containing struvite were markedly lower in 20 breeds, markedly higher in 1 breed, and not significantly different in 15 breeds when compared with crossbreds. Breed, age, and sex were associated statistically with CaOx or struvite urolithiasis. In conclusion, there appears to have been a long-term increase in the proportion of specimens of canine urinary calculi that contain CaOx as well as a long-term decrease in the proportion of specimens of calculi that contain struvite for both male and female dogs. The rate of change appeared to begin leveling off in the period 1998 to 2001. The recent proportion of dogs with either CaOx- or struvite-associated urolithiasis may depend on breed, age, and sex, and on interactions among these 3 factors. 相似文献
20.
<正>1.INTRODUCTION Ensuring transportation network security is one Of the most daunting challenges confronting homeland security agencies today.Significant research has been dedicated.To model and analyze the vulnerability of transportation systems,while notably fewer studies propose specific strategies for deploying defensive technologies to safeguard these systems. 相似文献