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1.
This study was to investigate the regulation of monoclonal antibodies against adipocyte membrane proteins （McAb） on lipid metabolism in pigs. Forty Landrace x Saba pigs were randomly divided into eight groups; the control group was given 10 mL saline and the treat groups were given monoclonal antibody against adipocyte-specific membrane protein with 0.1, 0.5, and 1.0 mg kg-I body weight at 15 and 60 kg body weight, respectively, by intraperitoneal injection. The results showed that McAb could increase, significantly, serum lipoprotein lipase activity and reduce serum nonesterified fatty acid （NEFA） content. Meanwhile, McAb increased content of serum lipid, triglyceride （TG）, cholesterol （CHO）, high density lipoprotein （HDL）, and low density lipoprotein （LDL） both at 15 and 60 kg body weight. However, McAb affected more significantly the lipid metabolism at 15 kg body weight than at 60 kg body weight. Moreover, this effect of McAb on lipid metabolism exhibited dose-dependent effect. These results suggested that this monoclonal antibody increased lipase activity, promoted lipolysis, and utilization of lipid so that McAb could be applied to restrain excessive fat deposition in porcine production through the regulation of fat metabolism.  相似文献   

2.
 通过动物试验测定抗脂肪细胞特异膜蛋白单克隆抗体对猪内分泌代谢的影响。结果表明：给15kg仔猪阶段腹腔注射0.1,0.5和1.0mg/kg体重抗体后，血浆Ins含量不同程度降低，注射1.0mg/kg体重抗体Ins含量与对照组差异显著（P＜0.05）；血浆T3,T4,GH,IGF-I含量不同程度提高，注射1.0mg/kg体重抗体这些指标的含量与对照组差异显著（P＜0.05）；给60kg肥育猪腹腔注射抗体后，血浆Ins,T3,T4,GH,IGF-I的变化趋势与仔猪相同，但变化幅度不如仔猪明显。表明抗脂肪细胞特异膜蛋白单克隆抗体可降低血浆Ins含量，提高血浆T3,T4,GH,IGF-I水平，对猪内分泌代谢有明显调节作用。  相似文献   

3.
To detect the effects of the polyclonal antibodies raised in sheep against porcine adipocyte plasma membranes on the porcine carcass composition and meat quality, 30 pigs assigned into 6 treatment groups were given intraperitoneal injections of sheep antipig adipocyte plasma membrane immunoglobulin （ASIg） or sheep nonimmune serum immunoglobulin （NSIg）. At the end of the experiment, the pigs were slaughtered at 90 kg body weight, and carcasses and meat quality were evaluated. The results showed that when pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, 20 mg purified ASIg twice at 15 and 60 kg body weight, or 20 mg purified ASIg at 60 kg body weight, respectively, their lean meat percentage, fat meat percentage, backfat thickness, loin eye area leaf fat weight, caul fat weight, heart weight, liver weight, and kidney weight were significantly affected. However, the kidney weight, lurrg weight, dressing percentage, and spleen weight did not remarkably change. Our results indicated that pigs intraperitoneally immunized with 20 or 30 mg ASIg at 15 kg body weight, and 20 mg ASIg twice at 15 and 60 kg body weight, have significantly different drip loss rate, cooked meat ratio, tenderness, storage loss rate, muscle fiber diameter, moisture content, dry matter content, crude protein content, and crude fat content from the control group that received 20 mg NSIg at 15 kg body weight. However, meat pH, meat color value, meat marbling score, inosinate, and myohemoglobin were not significantly affected. Our results indicated ASIg could not significantly affect the content of most muscular amino acids and intramuscular fatty acids.  相似文献   

4.
 给15kg体重仔猪分别腹腔注射0,10,20,30mg抗脂肪细胞膜蛋白多克隆抗体。结果显示,抗脂肪细胞膜蛋白抗体可显著提高血清脂蛋白脂酶（LPL）的活性和低密度脂蛋白（LDL）的含量（P<0.05）,降低游离脂肪酸（FFA）和极低密度脂蛋白（VLDL）的含量（P<0.05）,对血清总脂、甘油三酯（TG）、血清总胆固醇（CHO）和高密度脂蛋白（HDL）的含量无明显影响（P>0.05）。此外,该抗体可显著降低血清胰岛素（Ins）的水平（P<0.05）,提高三碘甲状腺原氨酸（T3）的水平（P<0.05）,而对血清生长激素（GH）和胰岛素样生长因子-1（IGF-1）的水平无明显影响（P>0.05）。表明抗脂肪细胞膜蛋白抗体可通过影响血液脂类代谢和激素的水平而减少体内脂肪沉积。  相似文献   

5.
 研究日粮不同能量水平对乌金猪脂肪组织中脂肪细胞分泌因子leptin和RBP4基因表达的影响。选取体重约15kg的乌金猪54头,随机分为3组,分别饲喂消化能为14.22,12.89,11.74MJ/kg的日粮,在30,60,100kg体重时屠宰,分别测定血液中胰岛素和葡萄糖含量及胴体脂肪沉积率,取皮下脂肪组织用荧光定量PCR检测leptin和RBP4基因的表达水平。结果显示,饲喂高能量日粮乌金猪胴体脂肪沉积率和血液胰岛素含量高于饲喂低能量日粮组（P<0.05）,日粮能量水平对血液葡萄糖含量无明显影响（P>0.05）;脂肪组织中leptin基因的表达水平升高（P<0.05）,RBP4基因的表达水平降低（P<0.05）。表明高能量日粮通过上调leptin基因的表达、下调RBP4基因的表达,和胰岛素的作用促进脂肪沉积。  相似文献   

6.
本文通过对山西马身猪不同体重阶段日均氮沉积量与猪体各组织器官平均日增重的灰关联分析表明：体重１０～９０千克阶段的马身肥育猪，影响氮沉积主要体内因素是肉、脂、骨，其次为内脏、消化道和皮。１０～６０千克阶段，各组织器官生长的增强有利于氮的沉积，关联序为肉→脂→骨→消化道→内脏→皮。体重大于６０千克时，肉、骨、皮生长的减弱、脂肪合成代谢的增强制约氮的沉积，关联序仍为肉→脂→骨，其余组织器官的关联序尚不能确定。体重６０千克左右，日均氮沉积量达到高峰。马身肥育猪体重１０～６０千克阶段应饲以较高能量和蛋白质的饲粮，６０千克阶段后应适当降低；适宜屠宰体重为８０千克。  相似文献   

7.
中草药与金霉素对生长猪的影响及其机理初探   总被引：5，自引：0，他引：5  
75～80日龄体重相近仔猪150头,随机均分3组,每组5个重复,每个重复10只样本,研究中草药饲料添加剂与金霉素影响生长猪生产性能的免疫内分泌机制。以中草药添加剂（1％）和盒霉素（25mg／kg）分别添加饲喂组Ⅰ,Ⅱ猪群,组Ⅲ为对照,各组仔猪注射猪瘟疫苗2头份／头。分别于开始用药当天（d0）、35d（d35）时逐一称重,并各组前腔静脉采血10头,统计各组猪群生产性能,测定血清猪瘟抗体水平和胰岛素样生长因子-Ⅰ（IGF—Ⅰ）、胰岛素（Insulin）和三碘甲腺原氨酸（T3）和甲状腺素（T4）等与生长有关的内分泌激素。结果显示中草药组仔猪在净增重、日增重和增重率及料肉比方面都显著优于金霉素组和对照组猪群,而后2组间差异不显著。试验结束时,中药组猪群血清猪瘟抗体水平、IGF—Ⅰ、Insulin和T4水平显著高于金霉素组和对照组仔猪。表明该中草药添加刑可通过增强仔猪免疫功能．并显著提高仔猪血清IGF—Ⅰ和胰岛素的水平、减缓T4的下降,从而提高仔猪的生产性能,表现出1种整体调节效应。  相似文献   

8.
中草药添加剂对生长肥育猪内分泌的影响研究   总被引：15，自引：2，他引：15  
 选用20 kg左右的“杜长大”三元杂交猪120头,分成对照组、中药组、中药+西药组,每组4个重复。测定中草药添加剂对生长育肥猪血清中GH,IGF-I,T3,T4和cAMP含量的影响。结果表明:在20～30 kg,30～70 kg,70～110 kg阶段,中药组、中+西药组与对照组相比,血清GH的浓度分别提高了7.34%（P>0.05）和5.50%（P>0.05）,21.09%（P<0.05）和16.32%（P<0.05）,7.56%（P>0.05）和6.40%（P>0.05）;IGF-I水平分别升高了15.32%（P<0.05）和13.86%（P<0.05）,17.20%（P<0.05）和13.82%（P<0.05),6.56%（P>0.05）和4.16%（P>0.05）。在20～30 kg,30～70 kg和70～110 kg阶段,T3含量分别增加了29.42%（P<0.05）和19.83%（P<0.05）,16.01%（P<0.05）和11.67%（P<0.05）,12.41%（P<0.05）和7.33%（P>0.05）;T4含量分别增加了23.31%（P<0.05）和14.32%（P<0.05）,17.06%（P<0.05）和11.51%（P< 0.05）,6.81%（P>0.05）和2.03%（P>0.05）。在20～30 kg,30～70 kg和70～110 kg阶段,cAMP含量分别提高了15.88%（P<0.05）和13.97%（P<0.05）,19.88%（P<0.05）和13.14%（P<0.05）,18.33%（P<0.05）和9.86%（P<0.05）.研究结果提示,中草药添加剂对生长育肥猪的促生长、改变胴体组成作用,可能是通过影响猪内分泌系统机能而实现的。似是一种整体效应,一方面通过提高饲料养分消化率而促进生长。另一方面,是通过促进GH,IGF-I的合成和分泌,加强合成代谢;同时促进T3,T4和cAMP的合成和分泌,促进脂肪的动员,加速脂肪的氧化和分解、降低猪体脂的积累有关。  相似文献   

9.
实验测定了哈尔滨白猪(简称哈白猪)56日龄经兽用排卵素二号处理后的外周血清促卵泡素和促黄体素的含量;测定了哈白猪、杜洛克猪及杜哈杂种猪4月龄、5月龄和6月龄外周血清孕酮含量;分析了这些内分泌性状、生长发育性状、酮体性状和繁殖性状的表型与遗传特点。实验结果表明,所测内分泌性状表现为中等或低于中等的遗传力(0.1055—0.3363);所测内分泌性状的遗传模型和生统模型为近似累积模型;4月龄外周血清孕酮含量与6月龄背膘厚之间表现为较强的遗传正相关(0.7932);4月龄和5月龄外周血清孕酮含量的遗传特点不同于6月龄外周血清孕酮含量的遗传特点,其原因可能与基因开放的顺序有关。  相似文献   

10.
试验选用84头10 kg左右杜洛克×长白×大约克(DLY)三元杂交阉公猪,随机分成2组,组内设6个重复,每个重复7头猪,研究了在NRC(1998)标准(对照组)、荣昌猪(GB 7223-1987)饲养标准(试验组)2种饲粮条件下营养水平对DLY猪生长肥育期背最长肌I MF含量、HSL及FAS mRNA表达量和血液激素含量的影响。结果表明:降低饲粮营养水平可降低35、80及110 kg DLY猪血清胰岛素含量和35及80 kg猪生长激素含量;同时可升高20、50及110 kg DLY猪血清肾上腺素含量和35、50及110 kg DLY猪胰高血糖素含量。各阶段试验组背最长肌肌内脂肪含量均高于对照组,DLY猪50 kg时达到了显著水平;除50 kg组外,其余各阶段试验组背最长肌HSL mRNA及FAS mRNA表达量均高于对照组;降低饲粮营养水平有升高20、35及80 kg DLY猪FAS mRNA/HSL mRNA的趋势。  相似文献   

11.
Production of monoclonal antibody against porcine adipocyte plasma membrane proteins to explore a new way of controlling body fat deposition and improving carcass quality is discussed in this article. Membrane proteins of pig adipocyte plasma membrane proteins were extracted with the help of sucrose density gradient centrifugation, and two kinds of proteins were obtained. The monoclonal antibody （designated 3B2 and 3F3） of IgG1 and IgG2b subclass against adipocyte membrane proteins were produced by immunization, with adipocyte membrane proteins as an antigen, and its titer was 1：105 detected by enzyme-linked immunoadsorbent assay （ELISA）. The cell strains were identified by analyzing the number of chromosomes, the heat stability, the acid and alkali, the types and subtypes of immnoglobulin, and its peculiarities and affinities. Through identification, the chromosome number of hybridoma cell strains was from 80 to 100 and the strains formed good hybridomas colonies. The strains＇ affinity constants were 4.63 × 10^9 and 3.75 × 10^9 （mol L^-1）-1, respectively. At the same time, the McAb secreted was stable to environmental factors, such as, temperature, acid, alkali and so on. The monoclonal antibodies had been obtained and their specificity to porcine adipocyte plasma membrane proteins had been identified.  相似文献   

12.
Specific endocrine tissue marker defined by a monoclonal antibody   总被引：25，自引：0，他引：25  
One of two mouse monoclonal antibodies (LK2H10) produced by hybridoma technology against a human endocrine tumor (pheochromocytoma) demonstrated specific immunoreactivity for 69 normal and neoplastic endocrine and tissues known to contain secretory granules. This immunoreactivity was specific, since other normal tissues, tumors from endocrine cells without granules, and tumors from other nonendocrine tissues were negative when tested with antibody LK2H10. The antibody reacted with human fetal adrenal medulla and human pancreatic endocrine cells and with adrenal medullary cells from monkeys and pigs. The antigen detected by antibody LK2H10 is associated with cytoplasmic secretory granules, has an estimated molecular weight of 68,000, and may be related to human chromogranin.  相似文献   

13.
研究测定85～107 kg体质量阶段27头鲁烟白猪的胴体品质和肉质特性,以分析鲁烟白猪胴体、肉质性状随体质量的变化规律及其相关程度.结果表明,鲁烟白猪在85～90 kg阶段屠宰瘦肉率最高为62.72%,分别比其他3组(91～95 kg组、96～100 kg组、101～107 kg组)提高1.62%(P>0.05)、0.34%(P>0.05)和2.40%(P>0.05);在91～95 kg阶段屠宰肉品质优良,肌肉pH6.11,肉色和大理石纹评分最高分别为3.26和 3.11,肌内脂肪含量最高为2.38%,肌肉失水率最低为13.89%.胴体和肉质性状间相关程度有强有弱,相关系数有正有负.相同性状间呈较强正相关,相反性状间呈较强负相关.在85～107 kg阶段,除屠宰率与体质量之间呈显著的回归关系外,其他各项指标与体质量间的线性相关不明显.综合分析认为,鲁烟白猪的适宜上市屠宰体质量为91～95 kg.  相似文献   

14.
Age, duration and intensity of feed restriction of broiler chickens were studied by L9(34) orthogonal experiment. Broiler production performance and meat color were measured to evaluate the effects of feed restriction and explore the superior FR program. A total of 250 1-day-old Ross 308 chicks were selected and randomly allocated to ten groups, including one feed ad libitum (AL) group and nine feed restriction (FR) groups; each group had 5 pens with 5 chickens each. Broilers (T5D7I90), feed-restricted to 90% from day 5 to day 11, had the highest body weight (BW), carcass weight, eviscerated weight, leg muscle weight and breast muscle weight, as well as muscle ratio. While broilers (T5D14I70), feed-restricted to 70% from day 5 to day 18, had the lowest. Triiodothyronine (T3) and growth hormone (GH) decreased while thyroxine (T4) and the ratio of T4/T3 increased in FR broilers. Feed conversion ratio, abdominal fat ratio, whole eviscerated weight ratio and lightness (L*) and yellow/blue (b*) value of leg muscle color increased in FR broilers (P> 0.05). T5D14I70 had the lowest red/green (a*) color value of leg muscle and the highest L* and b* color values of breast muscle (P> 0.05). FR groups except T10D14I90 had lower a* color value of breast muscle than AL. FR program of T5D7I90 resulted in superior production performance and higher capability of meat production. Broilers feedrestricted T5D14I70 were stunted with the lowest muscle growth capability and deteriorated in the breast muscle color. FR improved leg muscle color and increased broiler byproduct production. Lower T3 and GH concentration and higher T4/T3 ratio in plasma were related with the increased feed conversion rate and fat deposition in FR broilers; however, they had no relation with meat production and growth rate.  相似文献   

15.
有机铬添加剂对猪生产性能和肉质的影响   总被引：28，自引：1，他引：28  
研究了吡啶羧酸铬对育肥猪生产性能和肉质的影响。 36头体重 70kg的约克×长白杂交肥育猪随机分成二组 ,分别饲喂基础饲粮 (对照组 )和基础饲粮 +铬 2 0 0 μg/kg。每组 6个重复 ,每个重复 3头猪 ,试验至 10 0kg体重结束。结果表明 ,有机铬对育肥猪的日增重、日采食量和料肉比无显著影响。添加有机铬趋于提高眼肌面积和肉色评分 ,降低肌肉大理石纹评分和肌内脂肪含量 ;但可极显著提高猪肉的滴水损失。表明 ,在本试验条件下 ,有机铬对猪肉品质有一定的不良影响  相似文献   

16.
东北民猪和哈尔滨白猪毛的数量在初生猪和15公斤体重阶段没有显著差异。在60公斤体重以后各阶段东北民猪的毛量多于哈尔滨白猪的,毛量随着体重的增长而相对地减少。绒毛是东北民猪所特有,但初生猪没有绒毛。绒毛的数量在夏季稀少,冬季密布。粗毛的长度和粗变,随着体重的增加而加长和加粗。绒毛的粗度也随着体重的增加而加粗。东北民猪和哈尔滨白猪的真皮厚度初生时没有显著差异,随着体重增加而显著加厚。东北民猪的真皮厚得多,尤其在90公斤体重比60公斤体重的真皮加厚一倍。  相似文献   

17.
中草药添加剂对生长肥育猪血液生理生化指标的影响   总被引：24，自引：0，他引：24  
 选用20 kg左右的“杜长大”三元杂交猪120头,分成对照组、中药组、中药+西药组,每组4个重复。测定添加中草药添加剂,对生长育肥猪血清中TP,ALB,GLO,CHO,TG3,HDL,LDL和VLDL的含量以及GPT,GOT和ALP活性的影响。结果表明:中药组与对照组相比,在20～30 kg和30～70 kg阶段,血清TP含量分别增加了13.74%（P<0.05）和11.13%(P<0.05);GLO含量分别提高了35.79%（P<0.05）和19.88%（P<0.05）;CHO 含量分别提高了19.64%（P<0.05）和12.39%（P<0.05）;TG3含量分别提高了22.35%（P<0.05）和21.43%（P<0.05）;在70～110 kg阶段,血清TP,GLO,CHO和TG3含量各组之间差异不显著;ALB含量在不同生长阶段,各组之间差异都不显著（P>0.05）.在20～30 kg,30～70 kg和70～110 kg阶段,GPT活性分别提高了10.75%（P<0.05）,13.39%（P<0.05）和12.26%（P<0.05）;GOT活性分别提高了10.88%（P<0.05）,14.16%（P<0.05）和11.13%（P<0.05）;ALP活性分别提高了33.50%（P<0.05）,12.14%（P<0.05）12.21%（P<0.05）. LDL,VLDL的含量仅在70～110 kg阶段,分别比对照组高了18.51%（P<0.05）,14.85%（P<0.05）,在猪其它不同的生长时期,血清HDL,LDL和VLDL的含量,各组之间都无显著差异（P>0.05）。研究结果提示,中草药添加剂对生长育肥猪的促生长、改善胴体品质和肉品质的作用,可能是通过神经-内分泌系统来实现的,通过GH,IGF-I,T3,T4,cAMP等内分泌激素以及GPT,GOT及ALP等酶的协同作用,改善体内生理生化过程,使体内蛋白质代谢加强,氨基酸利用率提高;肝脏脂肪代谢加强,体脂肪沉积减少,肌间脂肪沉积增加。  相似文献   

18.
将中等营养水平下饲养的44头太原花猪分别在体重2、10、20、30、40、50、60、70、80、90、100千克阶段进行了屠宰,对其胴体、内脏器官的生长发育研究结果如下: 1 最佳屠宰体重是80—90千克,瘦肉率高达52.5％,脂肪率低于33.5％; 2 随着体重的增加,瘦肉生长是平稳的,而脂肪的生长则越来越强烈; 3 通过进一步选育及对后期生长控制能量研究,太原花猪可为市场提供瘦肉较多的商品猪肉; 4 脾脏在体重为80千克阶段出现奇特的生长高峰,是否为太原花猪的内脏生长的必然规律?有待进一步研究。  相似文献   

19.
研究表明,民猪的屠宰率60公斤以后低于哈白猪。民猪屠宰率低不是由于其胴长和胴宽不如哈白,而是由于臀部发育落后和肌肉生长缓慢。15—120公斤阶段肉脂骨皮的异速生长式(y=ax~b),脂肪生长系数的b值最大;肌肉次之,略小于1,但民猪的肌肉b值小于哈白;骨的b值最小;皮的b值大于骨,但民猪皮的生长系数b突出的大(超过1)。  相似文献   

20.
本文观察了3个体重年龄,3种不同饲养水平,2个品种猪肌组织的发育及其形态学特征。1.正常组织达到50公斤时,低维组哈白猪的肌纤维直径落后于同组民猪,差异显著。说明哈白猪对劣环境反应比民猪敏感,抗逆性比民猪差。2.猪出生后暂时营养失调,对肌组织不能造成退化性和坏死性变化而使肌纤维数减少,主要是影响肌间组织,经补偿后肌组织与正常组无差异。3.1级肌束纤维数为30～60条,而数束或十数束构成2级束。4.民猪和哈白猪背最长肌、股二头肌均为中间型纤维(氧化糖酵解型)组成。5.70公斤时,哈白猪和民猪的肌纤维生长还未得到成熟水平,不宜屠宰。  相似文献   

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