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水泡性口炎(Vesicular Stomatitis,VS)是由水泡性口炎病毒(Vesicular Stomatitisvirus,VSV)引起马、牛和猪的一种重要的传染病。临床表现为口腔粘膜、乳房和蹄部冠状带皮肤出现水泡和溃疡。VSV感染牛和猪时,在临床症状上极易与口蹄疫(FMD)、猪水泡病(SVD)、猪水泡疹(VES)混淆。且能够感染人。被国际兽疫局(OIE)列为A类传染病。因此,对VSV致病机理的研究有着重要的社会经济和公共卫生意义。本文从病原及其分子生物学特点、分布与生态学以及致病机理三个方面对水泡性口炎的研究进展进行综述。 相似文献
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猪水疱性口炎是由水疱性口炎病毒引起的高度接触传染性的病毒性疾病。其临床特征为猪的唇部,鼻及口腔等处发生水疱,并从口中不断向外流涎,有时常常还发生在蹄冠和趾间皮肤上,其症状主要以水疱为主。该病在全球许多地区造成广泛流行。近年来,由于产品贸易量的增加,猪水疱性口炎病毒也陆续的传入我国。由于该病与猪水疱病、猪口蹄疫和猪水疱性疹等病毒性疾病容易混淆,因此对该病做出准确地诊断与防制显得尤为重要。在VSV疫苗的研究方面,主要是灭活疫苗和弱毒疫苗的研究,而在新型疫苗的研究方面很少。本文主要综述了猪水疱性口炎病毒的基因及其疫苗的研究进展,为进一步了解和预防该病提供参考依据。 相似文献
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水疱性口炎是由水疱性口炎病毒(VSV)引起的人兽共患性传染病,在我国尚无可用疫苗。为研制安全有效的水疱性口炎疫苗,本研究以新城疫病毒(NDV)LaSota弱毒疫苗株反向遗传操作系统为基础,构建并拯救出表达VSV糖蛋白(GP)的重组病毒(rLa-VSV-G)。间接免疫荧光、激光共聚焦、western blot等试验证明VSV-GP在重组病毒中正确表达;体外致病试验结果表明,rLa-VSV-G保持了LaSota亲本株的低致病性和高滴度的鸡胚生长特性;rLa-VSV-G接种4周龄~5周龄BALB/c雌性小鼠后,可以诱导显著的VSV中和抗体反应。本研究表明,重组病毒rLa-VSV-G具有作为防控VSV储备性疫苗的潜力。 相似文献
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猪水疱性口炎是由水疱性口炎病毒引起的一类急性、热性人畜共惠传染病.其典型特征是口腔黏膜发生水疱,并从口腔流出泡沫样口涎,有时在蹄冠和趾间皮肤上亦发生水疱.到目前为止,该病已经在美洲、欧洲及非洲广泛流行.随着我国与世界各国动物及动物产品贸易的增加,猪水疱性口炎病传入我国的风险也随之加大,因此对该病及时准确地诊断与防制是非常关键和必要的.主要从病原、流行病学、临床症状、防治措施等方面对猪水疱性口炎进行了较全面的阐述,以期为科学防控该病提供参考. 相似文献
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Vesicular stomatitis viruses (VSVs) cause a condition known as vesicular stomatitis (VS), which results in painful lesions in equines, cattle, swine, and camelids, and when transmitted to humans, can cause flu-like symptoms. When animal premises are affected by VS, they are subject to a quarantine. The equine industry more broadly may incur economic losses due to interruptions of animal trade and transportation to shows, competitions, and other events. Equine owners, barn managers, and veterinarians can take proactive measures to reduce the risk of equines contracting VS. To identify appropriate risk management strategies, it helps to understand which biting insects are capable of transmitting the virus to animals, and to identify these insect vectors’ preferred habitats and behaviors. We make this area of science more accessible to equine owners, barn managers, and veterinarians, by (1) translating the most relevant scientific information about biting insect vectors of VSV and (2) identifying practical management strategies that might reduce the risk of equines contracting VSV from infectious biting insects or from other equines already infected with VSV. We address transmission risk at four different spatial scales—the animal, the barn/shelter, the barnyard/premises, and the surrounding environment/neighborhood—noting that a multiscale and spatially collaborative strategy may be needed to reduce the risk of VS. 相似文献
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J. Hernandez De Anda M.D. Salman J. Mason T.J. Keefe P.A. Webb J.S. Reif R. Akkina 《Preventive veterinary medicine》1992,14(3-4):209-215
A prospective study of vesicular stomatitis was conducted in two bovine herds in southeastern Mexico. In July 1987, an initial serological screening showed that 64% and 87% of the 654 cattle tested negatively to vesicular stomatitis New Jersey and Indiana antibodies, respectively, using the plaque-reduction serum neutralization test. Most seropositive animals were at least 24 months of age. Based on the initial serological screening, cohorts of seronegative and seropositive cattle were monitered (January–December 1988) for the prevalence of vesicular stomatitis virus (VSV) antibodies using an enzyme-linked immunosorbent assay (ELISA). The serological results, using ELISA, indicated that no VSV activity occured in the two study herds. The seronegative cohort of cattle did not yield a positive seroconversion pattern to either VSV Indiana or New Jersey. The seropositive cohort showed a variable antibody response pattern against the VSV. There were no clinical cases of vesicular stomatitis (VS) in the two herds. The data from the national surveillance program for vesicular diseases suggested that 1988 was a year of low VSV infection incidence in southeastern Mexico. 相似文献
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Kim L Morley PS McCluskey BJ Mumford EL Swenson SL Salman MD 《Journal of the American Veterinary Medical Association》2000,216(9):1399-1404
OBJECTIVE: To report clinical and serologic findings in horses with oral vesicular lesions that were consistent with vesicular stomatitis (VS) but apparently were not associated with VS virus (VSV) infection. DESIGN: Serial case study. ANIMALS: 8 horses. PROCEDURE: Horses were quarantined after appearance of oral lesions typical of VS. Severity of clinical signs was scored every 2 to 5 days for 3 months. Serum samples were tested for antibodies by use of competitive ELISA (cELISA), capture ELISA for IgM, serum neutralization, and complement fixation (CF). Virus isolation was attempted from swab specimens of active lesions. RESULTS: 2 horses with oral vesicular lesions on day 1 had antibodies (cELISA and CF) against VSV; however, results of CF were negative by day 19. Five of the 6 remaining horses were seronegative but developed oral lesions by day 23. Virus isolation was unsuccessful for all horses. CONCLUSIONS AND CLINICAL RELEVANCE: Horses were quarantined for 75 days in compliance with state and federal regulations. However, evidence suggests that oral lesions were apparently not associated with VSV infection. The occurrence in livestock of a vesicular disease that is not caused by VSV could confound efforts to improve control of VS in the United States and could impact foreign trade. Vesicular stomatitis is of substantial economic and regulatory concern. 相似文献
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An enzyme-linked immunosorbent assay for the detection of bovine antibodies to vesicular stomatitis virus 总被引：2，自引：0，他引：2
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibody to vesicular stomatitis virus (VSV). Serum samples from cows experimentally infected with the New Jersey serotype of VSV (VSV-NJ) were assayed by the ELISA and serum-neutralization (SN) assay. The ELISA was as sensitive as the SN assay in detecting bovine antibody to VSV. The correlation between SN titers and ELISA values at absorbance at 405 nm was statistically significant. The ELISA was not specific for VSV-NJ, however, and could detect serum samples positive to the Indiana serotype of VSV that had SN titers of greater than or equal to 480. Nonspecific reactions were due to cross-reactive group-specific viral proteins that are shared by both serotypes. The cross-reactivity allows the use of a single rapid test in identifying both serotypes of VSV from the other exotic vesicular diseases, especially foot-and-mouth disease. The ELISA titers of serum samples positive for VSV-NJ were comparable with the corresponding SN titers of each sample. The sensitivity, rapidity, and ease of the ELISA system and the use of a single test in identifying both serotypes of VSV from the other exotic vesicular diseases make this ELISA suitable as a rapid diagnostic assay for VS. 相似文献
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采用HE、Giemsa染色、透射电镜以及DNA琼脂糖凝胶电泳等研究了水泡性口炎病毒（VSV）诱导BHK-21细胞凋亡的过程。结果显示：VSV感染BHK-21细胞后,光镜下可见细胞圆缩,细胞器固缩、核仁消失、染色质凝聚和核碎裂、凋亡小体出现;电镜下观察到染色质聚集形成典型的新月形,胞浆中充满大量空泡,细胞核因染色质凝聚也发生了空泡化;1％的琼脂糖凝胶电泳出现180-200bp整倍数的DNA梯形条带。结果表明,VSV诱导BHK-21细胞凋亡是其致细胞病变的主要表现形式之一。 相似文献
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Jack PJ Amos-Ritchie RN Reverter A Palacios G Quan PL Jabado O Briese T Lipkin WI Boyle DB 《Veterinary microbiology》2009,133(1-2):145-153
Definitive diagnosis of vesicular or vesicular-like lesions in livestock animals presents challenges both for veterinary clinicians and diagnostic laboratories. It is often impossible to diagnose the causative disease agent on a clinical basis alone and difficult to collect ample vesicular epithelium samples. Due to restrictions of time and sample size, once laboratory tests have ruled out foot-and-mouth disease, vesicular stomatitis and swine vesicular disease a definitive diagnosis may remain elusive. With the ability to test a small quantity of sample for a large number of pathogens simultaneously, DNA microarrays represent a potential solution to this problem. This study describes the application of a long oligonucleotide microarray assay to the identification of viruses known to cause vesicular or vesicular-like lesions in livestock animals. Eighteen virus isolates from cell culture were successfully identified to genus level, including representatives of each foot-and-mouth disease virus serotype, two species of vesicular stomatitis virus (VSV), swine vesicular disease virus, vesicular exanthema of swine virus (VESV), bovine herpesvirus 1, orf virus, pseudocowpox virus, bluetongue virus serotype 1 and bovine viral diarrhoea virus 1. VSV and VESV were also identified in vesicular epithelium samples, with varying levels of sensitivity. The results indicate that with further development this microarray assay could be a valuable tool for the diagnosis of vesicular and vesicular-like diseases. 相似文献