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猪伪狂犬病油乳剂灭活疫苗的制备及安全性与免疫性试验 总被引：12，自引：0，他引：12
用本室分离鉴定的猪伪狂犬病毒鄂A经毒株接种仓鼠肾细胞（BHK－21）,制备病毒抗原液，毒价不低于10^-6TCID50/0.1ml。经一定浓度的甲醛溶液灭活后与油相佐剂乳化研制成油乳剂灭活油疫苗4批。本研究对该制品的安全性、免疫性进行了测定。对18g左右小白鼠接种0.3ml，初生仔猪、断奶仔猪及妊娠母猪加倍剂量注射，均未出现不良反应，安全性良好。对母猪的繁殖性能不产生影响。后备母猪及妊娠母清中和抗体指数于免疫后21d达到316以上，间隔35d加强免疫一次后，中和抗体指数可达1000以上。断奶仔猪及初生仔猪免疫后对强毒的攻击，保护率分别为100％及90.62％。 相似文献
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《饲料工业》2016,(9)
试验旨在研究不同水平的VE(44 IU/kg和250 IU/kg)和不同脂肪类型(3.5%豆油和3.5%亚麻籽油)对仔猪生长性能及脂肪酸组成的影响。采用2×2因子试验设计,选择健康、体重相近的经产母猪(长白×大白,3~4胎)48头,随机分成4个处理组,每组12头母猪。试验从母猪妊娠107 d开始持续至仔猪断奶(哺乳期21 d)结束,约28 d。结果表明,添加高水平的VE(250 IU/kg)显著提高了仔猪断奶重和平均日增重(P0.05),添加亚麻籽油显著提高了仔猪断奶重和平均日增重(P0.05),添加亚麻籽油能显著提高母猪乳、仔猪血浆和脑组织中n-3 PUFA的比例(P0.05)。添加VE能显著提高母猪乳、仔猪血浆及脑组织中VE的含量(P0.05)。在母猪妊娠后期和哺乳期添加亚麻籽油和VE能够提高仔猪生长性能及改变仔猪组织中脂肪酸的组成。 相似文献
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为了观察促排卵素3号（LRH-A3）对母猪繁殖性能的作用和效果，发情母猪配种前4—6h，用LRH-A3进行处理，并与不使用激素的发情母猪进行比较。结果地方品种母猪的受胎率、平均产仔数、仔猪成活率分别为93．3％、14．6头和89．5％。与对照组相比，母猪受胎率无显著差异（P〉0．05），平均产仔数显著高于对照组（P〈0．01），仔猪成活率比对照组低4．6％，差异显著（P〈0．01）。经产瘦肉型母猪的配种受胎率、平均产仔数、仔猪成活率分别为86．7％，12．3头和93．8％，与对照组相比，配种受胎率、仔猪成活率无显著性差异（P〉0．05），母猪平均产仔数显著高于对照组（P〈0．01）。青年瘦肉型母猪的配种受胎率、平均产仔数、仔猪成活率分别为83．3％、10．6头和94．5％，与对照组相比，母猪的配种受胎率、平均产仔数均显著高于对照组（P〈0．01），仔猪成活率与对照组无显著差异（P〉0．05）。试验结果表明，发情母猪配种前肌注LRH-A3后，青年瘦肉型母猪繁殖效果最理想，经产瘦肉型母猪次之，地方品种母猪不理想。 相似文献
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总计84头母猪(PIC,Line1050)和它们所生仔猪被用于本实验,以确定泌乳料和教槽料采食对母猪和仔猪性能的影响。母猪按照分娩日期和胎次被分为3组,并按照2×2因子实验:母猪采食(自由采食与限饲)和仔猪采食(不喂仔猪料与喂仔猪料)共4个处理。仔猪被交叉寄养于每个实验组,以均衡窝重和窝仔猪数(>11头仔猪)。实验采用的是一种普通泌乳母猪料(ME 14.65MJ/kg,TID 0.97%lys),产仔后3d自由采食组母猪开始自由采食,限饲组母猪饲喂比自由采食组少25%的泌乳母猪料。饲喂仔猪料组仔猪采用的是加有1%氧化铬标记物的颗粒料(ME 14.64MJ/kg,TID 1.56%Lys),从3日龄喂到21日龄断奶。仔猪分别于3、7、14、21日龄称个体重,并每天计量仔猪采食量。在7、14、21日龄采集所有喂仔猪料的仔猪的粪便样本,同时通过仔猪粪便颜色区分采食仔猪料的仔猪和未采食仔猪料的仔猪。母猪在分娩后和断奶时称重,并测量P2点(距背中线6.5cm,最后肋骨)背膘。泌乳料采食和仔猪料采食没有相互作用,自由采食组母猪减少了体重损失(-15kg与-24kg;P<0.01),提高了仔猪窝重(P<0.04)和日增重(P<0.04),并比限饲组提高了断奶后14d内发情率(90%与71%;P<0.03)。饲喂仔猪料对母猪体重、背膘损失无影响(P>0.30),但会拖后母猪断奶后14d内发情间隔(5.4d与4.9d;P<0.09)。饲喂仔猪料有改善断奶窝重(61.2与56.7kg/d;P<0.09)和减少交叉寄养后仔猪死亡率(3.9%与7.3%;P<0.06)的倾向。自由采食母猪组和限饲母猪组仔猪采食仔猪料量差异不明显(2.24与2.28;P<0.93)。喂仔猪料组大约60%仔猪被确定采食了仔猪料,在被确定采食仔猪料的仔猪中有23%、20%和57%开始采食分别从3～7日龄、7～14日龄、14～21日龄。从断奶后0～28d,饲喂仔猪料与不喂仔猪料组在断奶后28d重(P<0.95)、日增重(P<0.86)、日采食量(P<0.93)和料重比(P<0.95)差异不显著。采食仔猪料的仔猪在断奶后28d重(P<0.16)和日增重(P<0.06)方面好于不吃仔猪料和不喂仔猪料仔猪。结论:喂仔猪料可以改善仔猪成活率但不能改进仔猪断奶前增重和母猪性能表现。泌乳母猪采食量低会对母猪性能和仔猪性能产生负面影响。让更多的仔猪采食仔猪料对改善仔猪断奶后性能是有利的。 相似文献
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Beran GW 《Preventive veterinary medicine》2008,86(3-4):198-207
When early people made their appearance, zoonotic infectious diseases were already waiting, but epidemic diseases did not appear in human history until people began to live in large numbers under conditions of close contact, mainly during the last 10,000 years. Disease has decimated urban populations, conquered armies, and disrupted society. The focus here is on (1) the plague of Athens and the Black Death; (2) smallpox, influenza, and rabies; (3) avian influenza prion diseases, and foot & mouth disease; and (4) emerging and re-emerging diseases. All have veterinary public health associations. In Athens, Greece, in 430 BC, when the Spartans ravaged the countryside, hordes crowded into Athens so that orderly movements, space in which to live, and adequate supplies of food became impossible. Crowding of any population fosters disease transmission; chaos and disorder enhance it all the more. Out of northern Egypt came a terrible plague from across the Mediterranean Sea. The identity of the plague of Athens remains unsure, but the well-considered conclusion is Rift Valley Fever, a mosquito borne, viral zoonosis. The Black Death, also called the Plague, raged in Asia for centuries. In 1347, the Black Death was brought by a ship out of Asia to Sicily. The scenes of devastation were repeated throughout Europe, with 90% or more of the people dying in city after city. Influenza, too, has been a cause of periodic human epidemics, but the great pandemic of influenza occurred in the last months of World War I. In the years of highest occurrence, more than half the world's population became clinically infected. If veterinary public health had been born earlier, it could have led to elucidating the epidemiology of influenza and the plagues of Athens, Europe, and Asia. In turn, smallpox had also caused continual tragedy. In 1796, Edward Jenner began to harvest pustules of cowpox from children or infected cows and inject them into susceptible children. In 1980, the World Health Organization declared that smallpox had been eliminated from the world. Rabies, though, still strikes terror. A number of animal diseases, broadly termed emerging and re-emerging diseases, need surveillance because they have the potential to impact human health. From late in 2003 to 2007, the highly pathogenic H5N1 influenza virus in poultry infected at least 121 people and caused 62 deaths in four countries. The prion diseases, too, all have very high numbers in concentrated contacts. To control these diseases, veterinary public health is essential, with diagnosis, epidemiological surveillance, clinical manifestations, and prevention as primary measures. 相似文献
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Dekker A Dercksen D Snoep J van Wuyjckhuise L 《Tijdschrift voor diergeneeskunde》2007,132(18):695-701
On 26th of july 2007 a new case of bluetongue was notified in the Netherlands and on 2nd of august 2007 foot-and-mouth disease was diagnosed in Surrey, England, which raised the threat of having both infections simultaniously in one area. Bluetongue and foot-and-mouth disease have a different pathogenesis, but symptoms may resemble each other at a later stage of infection. The pathogenesis and possible clinical symptoms of both infections are discussed and illustrated with pictures. 相似文献
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在动物的异常行为中以刻板行为最为常见,而咽气癖又是马最常见的口部刻板行为之一。作者就咽气癖在生理方面和心智方面对马体产生的影响、行为基础、诱因的研究进展及常见的防治措施进行综述。 相似文献
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