共查询到18条相似文献，搜索用时 296 毫秒
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本文建立了一个微弹介导的火炬松遗传转化系统。这个系统解决了火炬松遗传转化过程中存在的许多困难。运载抗虫基因的质粒载体经微弹转化法进入火炬松成熟合子胚,然后在添加了卡那霉素的培养基上从转化的成熟胚上诱导出有器官发生潜力的愈伤组织,再从转化的愈伤组织上产生转基因植株。利用这一系统生产的转基因植株已经被随机扩增技术、Southern杂交技术和虫试验所证实,并且转化的植株已在土壤中成活。图3表2参28。 相似文献
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表达耐盐基因的转基因火炬松的再生 总被引：2，自引：0，他引：2
盐害是限制作物和树木分布和生产的重要因素。盐分过多导致细胞内水分缺失并影响许多重要的细胞代谢活动。本文利用火炬松作为模式植物建立了一套提高植物耐盐性的新技术。这一技术以火炬松合子胚为材料,利用农杆菌介导的转化方法将山犁醇脱氢酶和甘露醇脱氢酶基因转入火炬松。然后再生转化的愈伤组织和转基因植株。经DNA杂交证实的转基因植株被用于耐盐性试验,结果表明这些转基因的植株的耐盐性有明显的提高。这一技术对针叶树的遗传工程育种有重要的参考价值。图3表2参26。 相似文献
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山东省针叶树种(球果类)遗传改良及评价 总被引：1，自引：0，他引：1
山东省林木遗传改良从建国后可以分成初始阶段(50~60年代)、全面发展阶段(70~80年代)和常规育种与生物技术相结合阶段(90年代以后)。近35年来山东林业科技针叶树遗传改良论文占3.06%,总体呈下降的趋势。本文对我省针叶树育种场建设、种源试验和种源选择、优树选择、杂交育种、子代测定、良种快繁技术、针叶树遗传改良项目、成果及品种等进行了回顾。同时对针叶树遗传改良策略、种源选择、种子园与遗传增益、现代育种技术在针叶树遗传改良上的应用和杂交育种等进行了讨论。 相似文献
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木麻黄科植物具有特殊的生物学特性、较高的经济价值和广泛的社会效益,作为重要的防护林、用材林和多用途林树种,是重要的热带林木种质资源。由于可持续性发展的需要,木麻黄研究越来越受到人们的重视,木麻黄遗传改良方面已取得显著成果。近十年来分子生物学技术的飞速发展,尤其是植物基因克隆和转化研究的日新月异,极大地促进了木麻黄转基因研究的发展,缩短了木麻黄育种改良周期,为木麻黄—微生物共生体研究提供了有效的方法。木麻黄分子生物学研究始于20世纪80年代晚期,主要进行了木麻黄细胞学基础、种群遗传多样性、固氮基因分离和克隆、基因转移等方面的研究,目前已取得了令人瞩目的成果。文章综述了国内外木麻黄科植物在分子生物技术方面的研究及进展,提出了该领域研究的需求和前景,以期对该领域的深入研究起到积极的促进作用。 相似文献
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Many important advances in forest biotechnology have been made. The use of genetic transformation and the applications of
transgenic trees in modern forestry is now an important field. Two basic methodologies particle bombardment andAgrobacterium-mediated transformation have been used on conifers. However, routine procedures exist for only a limited number of conifers.
As a result only a few species have been successfully transformed into stable transgenic plants. The use of a particle bombardment
has been more successful and transgenic plants have been produced inPicea abies, Picea glauca, Picea mariana, andPinus radiata, although the level of production of stable transgenic plants is lower than that ofAgrobacterium. At present, breeding programs have been directed toward improving bole shape, growth rate, wood properties, and quality,
as well as toward improving root and shoot performance, pest resistance, stress tolerance, herbicide resistance, and ability
to resist stresses, which will drive forestry to enter a new era of productivity and quality. This article provides a brief
overiew of the current state of knowledge on genetic transformation in conifers.
Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, O-23, E. S. King Village, 2900, Ligon St., North Carolina State
University, Raleigh, NC 27607, USA
Responsible editor: Chai Ruihai 相似文献
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林木遗传工程及木质素的生物合成调节(英文) 总被引：1，自引：0，他引：1
林木遗传工程有利于保存林木遗传资源，改善全球气候，减轻自然林的过度采伐和满足人类日益增长的林木产品需求。控制林木真菌、病毒病、虫害和杂草的遗传工程方法正被广泛地研究和应用。尽管转基因林木的历史不长，种类不多，但它有广泛的应用前景。目前，抗除草剂基因、抗虫基因以及和木材质量相关的基因已被分离并应用于林木遗传工程。植物分子生物学和基因组学中的新技术使得高效林木遗传改良成为可能并将促进这些技术的商业化应用。木质素的应用已有一百年的历史，但木质素生物合成的全过程并不完全清楚。有关松树自然突变体和转基因林木的最新研究结果表明，木质素的生物合成是一个可以调节的过程。这些发现对完善木质素的生物合成途径、加深对木质素前体生物合成途径的理解和通过遗传工程改善木材质量有促进作用。本文综述了林木遗传工程在这些领域中取得的一些进展。 相似文献
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《Scandinavian Journal of Forest Research》2012,27(2):151-160
A particle inflow gun enabled efficient production of transgenic plantlets of Picea abies from embryogenic suspension cultures. In transient assays, the Zea ubiquitin promoter was 12-16 times as active as the 35S promoter. For stable transformation, the plasmid pAHC25 contained the bar gene and the gusA gene, both driven by the Zea ubiquitin promoter. Cells were maintained from 1 to 3 h before bombardment on proliferation medium supplemented with 0.25 M myoinositol and, from day 8, supplemented with Basta as selective agent. Embryogenic colonies resistant to Basta appeared from two months after bombardment. Of over 100 independent Basta-resistant sublines tested, 65% expressed the co-transformed reporter gene, even when it was not linked to the selectable marker. Over 80% of the sublines retained their embryogenic potential. Of 11 transformants analyzed, 4 contained transgenes in low copy number (1-3), the rest contained transgenes in up to 15-20 copies. Over 200 Basta-resistant sublines from four cell lines have been established, of which 138 are confirmed as transformed. Plantlets have been regenerated and grown on in pots. 相似文献
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We used in vitro callus and shoot cultures as target material for genetic transformation of silver birch (Betula pendula Roth) by particle bombardment. Cultivation of in vitro shoot cultures before particle bombardment and a long selection period, combined with a high concentration of selective agent after bombardment, led to the production of transformed plantlets that were stable, and no escapes were found among the tree lines produced. Clonal variation in transformation efficiency was found in transient expression of the beta-glucuronidase gene in callus cultures and in plantlets transformed by stable integration of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (RbcS) and neomycin phosphotransferase (npt2) genes. 相似文献
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许多不同的技术可以促进农业生产力的持续增长，生物技术是其中最重要和最有前途的方法。因为生物技术不仅可以增加生产力。而且在环境和自然资源的保护中起重要作用，应用生物技术可以生产高产、优质、高抗性和无病植物，同时也可以提高育种效率和促进食品的工业化生产。苏云金杆菌抗虫基因是目前生物技术领域中应用最广泛的例子之一。由此而产生的抗虫植物正在广泛应用于世界各地的商业化生产。本文综述了苏云金杆菌抗虫基因的研究进展和它在未来林木遗传工程中的应用。表4参68。 相似文献
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利用农杆菌介导法获得转codA基因麻竹再生植株的研究 总被引：2，自引：0，他引：2
温度是影响植物生存和生长发育的基本环境因子之一。大多数植物对低温等都是高度敏感的,低温伤害现象尤为突出,几乎涉及所有的经济植物。因此,改善植物的抗低温冻害的胁迫能力,可以显著提高植物的生长范围、增加产量。codA基因可以增加植物对低温胁迫的耐受能力,而Rd29A是一种胁迫诱导特异表达启动子,胁迫条件可以快速诱导基因表达,也可以减少由于转基因过量表达带来的不利影响。研究以麻竹花药离体培养的愈伤组织为材料,采用农杆菌介导法,探讨了影响麻竹愈伤组织遗传转化效率的主要因子。结果表明,潮霉素的最佳筛选浓度是25 mg.L-1,预培养时间为3 d,侵染时间为20 min,共培养时间为3 d,乙酰丁香酮的浓度控制在100 mg.L-1时可以有效的提高遗传转化效率。在此基础上对获得的转基因植株进行分子检测,初步表明外源基因codA已经整合到麻竹基因组中。 相似文献
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Agricultural productivity may be raised in a sustainable way by many different technologies such as biological fertilizers,
soil and water conservation, biodiversity conservation, improved pest control, and changes in land ownership and distribution.
Of these measures, biotechnology applications probably hold the most promise in augmenting conventional agricultural productivity,
because biotechnology applications give not only the need to increase production, but also protect the environment and conserving
natural resources for future generations. Biotechnology applications will have the possibilities to increase productivity
and food availability through better agronomic performance of new varieties, including resistance to pests; rapid multiplication
of disease-free plants; ability to obtain natural plant products using tissue culture; diagnosis of diseases of plants and
livestock; manipulation of reproduction methods increasing the efficiency of breeding; and the provision of incentives for
greater participation by the private sector through investments. Insect resistance through the transfer of a gene for resistance
fromBacillus thuringiensis (Bt) is one of the most advanced biotechnology applications already being commercialized in many parts of the world. This paper
reviews the development and the status ofBt technology and application ofBt transgenic plants in current agriculture, and discusses specific issues related to the transfer of the technology to the
future of genetic engineered trees with emphasis on conifers.
Biography: Tang Wei (1964-), male, Ph. Doctor, Research associate, Department of Biology, Howell Science Complex, East Carelina University,
Greenville, NC 27858-4353, USA.
Responsible editor: Chal Ruihai 相似文献
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The gene coding for green fluorescent protein (GFP) from the jellyfish Aequorea victoria was successfully used as a vital marker for the transformation of three woody plant species, black spruce (Picea mariana (Mill.) BSP), white pine (Pinus strobus L.) and poplar (Populus spp.). The gfp gene and the gene conferring resistance to kanamycin (nptII) were introduced by microprojectile bombardment or Agrobacterium tumefaciens-mediated technology. Screening by fluorescence microscopy of the transformed plant material, under the selection of kanamycin, identified five to eight cell lines from each tree species that clearly expressed GFP. Expression of GFP was observed in somatic embryonal cells of the coniferous species and in stem sections of poplar. For all species, GFP transgene expression was stable over multiple subcultures. Stable integration of the gfp gene into plant genomes was confirmed by Southern hybridization or polymerase chain reaction (PCR) analysis. We conclude that GFP can be used as a vital marker and reporter protein in transformation experiments with gymnosperms and deciduous trees. 相似文献