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1.
采用聚丙烯烯酰胺凝胶电泳法对58峰青海双峰驼的红细胞和血清乳酸脱氢酶(LDH)以及红细胞超氧化物歧化酶(SOD)同工酶酶谱进行了分析和研究,结果:(1)SLDH同工酶酶谱存在年龄差异,成年驼只有LDH1同工酶,幼年驼酶谱由LDH1,LDH2,LDH3和LDH4组成;(2)RBC-LDH同工酶由LDH1,LDH2和LDH4组成;(2)RBC-LDH同工酶由LDH1,LDH2和LDH3组成,其活性强于  相似文献   

2.
采用聚丙烯酰胺凝胶电泳法对2只兔狲的4种血液蛋白质表型和3种血液同工酶酶谱进行研究。结果发现:①被检兔狲的血红蛋白(HB),白蛋白(ALB)和后白蛋白(Pa)分别呈单一的HBAB,ALBAA和PaAA型,运铁蛋白(TF)有TFAB和TFBB两种表型;②血清碱性磷酸酶(ALP)由ALPA,ALPB,ALPC和ALPD四种同工酶组成;③血清酯酶(ES)由ESI和ESIV两种同工酶共7条区带组成;④血清乳酸脱氢酶(S-LDH)由LDH1,LDH2,LDH3,LDH4和LDH5五种同工酶组成,红细胞内只有前四种LDH同工酶。  相似文献   

3.
青海细毛羊红细胞乳酸脱氢酶同工酶的研究   总被引:2,自引:0,他引:2  
采用聚丙烯酰胺凝胶电泳法对110只青海细毛羊的红细胞乳酸脱氢酶(RBC-LDH)同工酶酶谱及其多态性进行了研究。结果发现:青海细毛羊RBC-LDH同工酶总共有12条区带;第1条区带“1-A”的电泳迁移率为55.5%,第12条区带“11”的电泳迁移率为95.0%;RBC-LDH有两种电泳表型,108只羊为I型,占98.2%,2只羊为Ⅱ型,占1.8%;RBC-LDH1型尚可区分为两个亚型:I-BB型和  相似文献   

4.
黑白花奶牛LDH同工酶的发育遗传学分析   总被引:1,自引:0,他引:1  
黑白花奶牛血清LDH同工酶以B型酶占相对优势(58.7%-92.0%),LDH总活力以及各同工酶的表现形式和A、B两亚基的表达程度随机体生理状态的改变和个体生长发育阶段的不同而有变动。它们的这种表达特征是Idh a和Idh b两基因实施调控的结果,与奶牛代谢过程中的能量需求相适应。  相似文献   

5.
采用聚丙烯酰胺凝胶电泳法对21条藏獒的红细胞乳酸脱氢酶(RBC-LDH)和血清到脱氢酶(S-LDH)同工酶酶谱及其多态性进行研究。结果发现:(1)RBC-LDH同工酶共有3条区带,呈现两种电泳表型,11条藏契为LDH2A型(57.89%),8条藏獒为LDH2a(42.11%),(2)S-LDH共有5条区带,呈现两种电泳表型,2条藏獒为LDH1A(9.52%),19条藏獒为LDH1a(90.48%)  相似文献   

6.
东方铃蟾乳酸脱氢酶多态性研究   总被引:1,自引:0,他引:1  
采用聚丙烯酰胺凝胶电泳分离东方铃蟾不同组织LDH同工酶,显示出典型的5条带谱。心肌具有以B亚基为主的LDH同工酶谱,骨骼肌以A亚基为主的LDH同工酶谱。用改进后的聚丙烯酰胺凝胶电泳方法进行种群调查,结果表明A位点和B位点都存在电泳上的变异,各存在二个等位基因。A位点有三个基因型,其频率;AA=53.47%.Aa=39.11%.aa=7.43%;B位点只检出二个基因型,即BB为99.50%,Bb为0.50%。  相似文献   

7.
西藏牦牛血清乳酸脱氢酶H亚基的遗传变异体   总被引:10,自引:0,他引:10  
采用聚丙烯酰胺凝胶电泳分析西藏嘉黎和亚东地区85头牦牛血清乳酸脱氢酶(LDH)同工酶。结果显示,牦牛血清LDH同工酶具有LDH1 ̄LDH5五种形式,LDH的H亚基存在A、B、C三种遗传变异体,C型遗传变异体平均占82.35%。值得注意的是,嘉黎地区具有A或B型H亚型的牦牛血清中LDH2的相对活力显著低于具有C型H亚基的牦牛,其机制和意义有待进一步研究。  相似文献   

8.
用聚丙烯酰胺凝胶电泳对蛭形巨吻棘头虫(Macracanthorhynchushirudinaceus)雄虫、雌虫体液和宿主(猪)血清的LDH同工酶的酶谱、相对迁移率和相对活性等进行比较表明,雄虫有3条酶带和雌虫有2条酶带显示出很强的特异性;不同浓度的抑制剂(硝硫氰醚)对雄虫和雌虫特有的LDH同工酶酶带活性具有不同程度的抑制作用,尤其是雌虫第2带对抑制剂最敏感。蛭形巨吻棘头虫体液具有特异的且不同于宿主(猪)血清LDH1~5的LDH同工酶酶带,特有的LDH同工酶可能由4条不同的区带组成。  相似文献   

9.
应用聚丙烯酰胺凝胶电泳法对猪和绵羊的心、肝、脾、肺、肾、胰、小肠7种组织器官及血清的乳酸脱氢酶同工酶酶谱进行比较研究,结果发现:①猪和绵羊各组织器官中LDH同工酶共有5种区带,猪的LDH3同工酶还可分为两条亚带;②猪和绵羊的肺、肾、小肠、胰、血清中LDH区带数相同,其它组织器官各不相同;③猪和绵羊LDH1同工酶的泳动速度最快,迁移率分别为53.64%、41.82%;LDH5同工酶的泳动速度最慢,迁  相似文献   

10.
牦牛卵泡液LDH同工酶及外源性激素对其表达模式的影响   总被引:1,自引:0,他引:1  
采用比色法和不连续缓冲系统的聚丙烯酰胺凝胶电泳(PAGE)技术对未孕和怀孕初期牦牛卵泡液乳酸脱氢酶(LDH)活性及同工酶进行了测定,并分析了促卵泡素3号(LRH-A3)、人绒毛膜促性腺激素(hCG)和孕马血清促性腺激素(PMSG)对其表达模式的影响。结果表明,未孕和怀孕初期牦牛卵泡液LDH活性分别为200.4±31.1,215.3±86.6μmol·s-1·L-1,同工酶有5条谱带。3种外源性激素均使牦牛卵泡液LDH活性显著升高(P<0.01),LDH同工酶带型分布发生变化,降低了LDH2/LDH1比值,改变了同工酶中A和B亚基所占比例  相似文献   

11.
1-(2-Chloroethyl)3-cyclohexyl-1-nitrosourea (CCNU) is an alkylating agent in the nitrosourea subclass. A prospective evaluation of CCNU was done to determine the maximally tolerated dosage of CCNU in tumor-bearing cats. Response data were obtained when available. Twenty-five cats were treated with CCNU at a dosage of 50-60 mg/m3 body surface area. Complete hematologic data were available for 13 cats. Neutropenia was the acute dose-limiting toxicity. The median neutrophil count at the nadir was 1,000 cells/microL (mean, 2,433 cells/microL; range, 0-9,694 cells/microL). The time of neutrophil nadir was variable, occurring 7-28 days after treatment, and counts sometimes did not return to normal for up to 14 days after the nadir. Based on these findings, a 6-week dosing interval and weekly hematologic monitoring after the 1st treatment with CCNU are recommended. The nadir of the platelet count may occur 14-21 days after treatment. The median platelet count at the nadir was 43,500 cells/microL. No gastrointestinal, renal, or hepatic toxicities were observed after a single CCNU treatment, and additional studies to evaluate the potential for cumulative toxicity should be performed. Five cats with lymphoma and 1 cat with mast cell tumor had measurable responses to CCNU. Phase II studies to evaluate antitumor activity should be completed with a dosing regimen of 50-60 mg/m3 every 6 weeks.  相似文献   

12.
Human and equine athletes are reported to have a high prevalence of gastric disease, and anecdotal evidence suggests a similar phenomenon applies to racing sled dogs. To investigate the prevalence of gastric disease in racing sled dogs, we conducted 2 gastroscopy studies on dogs competing in the annual Iditarod Sled Dog Race. A pilot study of dogs that were either dropped from the 2000 Iditarod Sled Dog Race because of illness or that finished the race indicated that, approximately 5 days after competing, 10 of 28 dogs (35%) had endoscopic evidence of gastric ulceration, erosion, or hemorrhage. The next year, an endoscopic study of 73 dogs participating in the 2001 Iditarod race was performed in order to evaluate a larger population of dogs. Data from 70 of these dogs could be used; 34 (48.5%) had ulceration, erosion, gastric hemorrhage, or some combination of these findings. When this group of 70 dogs was compared retrospectively to a control group of 87 dogs presented to the Texas A&M University (TAMU) Veterinary Medical Teaching Hospital, the Iditarod sled dogs had a significantly higher prevalence (P = .049) of gastric lesions. These findings suggest that, similar to athletes of other species, elite canine athletes have an increased prevalence of gastric disease compared to the canine population at large.  相似文献   

13.
Continuous rate infusion (CRI) of furosemide in humans is considered superior to intermittent administration (IA). This study examined whether furosemide CRI, compared with IA, would increase diuretic efficacy with decreased fluid and electrolyte fluctuations and activation of the renin-angiotensin-aldosterone system (RAAS) in the horse. Five mares were used in a crossover-design study. During a 24-hour period, each horse received a total of 3 mg/kg furosemide by either CRI (0.12 mg/kg/h preceded by a loading dose of 0.12 mg/kg IV) or IA (1 mg/kg IV q8h). There was not a statistically significant difference in urine volume over 24 hours between methods; however, urine volume was significantly greater after CRI compared with IA during the first 8 hours ([median 25th percentile, 75th percentile]: 9.6 L [8.9, 14.4] for CRI versus 5.9 L [5.3, 6.0] for IA). CRI produced a more uniform urine flow, decreased fluctuations in plasma volume, and suppressed renal concentrating ability throughout the infusion period. Potassium, Ca, and Cl excretion was greater during CRI than IA (1,133 mmol [1.110, 1,229] versus 764 mmol [709, 904], 102.7 mmol [96.0, 117.2] versus 73.3 mmol [65.0, 73.5], and 1,776 mmol [1,657, 2.378] versus 1,596 mmol [1,457, 1,767], respectively). Elimination half-lives of furosemide were 1.35 and 0.47 hours for CRI and IA, respectively. The area under the excretion rate curve was 1,285.7 and 184.2 mL x mg/mL for CRI and IA, respectively. Furosemide CRI (0.12 mg/kg/h) for 8 hours, preceded by a loading dose (0.12 mg/kg), is recommended when profound diuresis is needed acutely in horses.  相似文献   

14.
The epidemiology of H. placei and of other gastrointestinal nematodes in yearling dairy cattle was examined on two farms in Kiambu District, central Kenya during each of 13 one-month periods from April 1993 to April 1994. On each farm, 32 newly weaned dairy calves were given a single dose of albendazole and then placed on experimental pastures. Twelve of the animals were designated for bi-monthly slaughter (n = 2) and analysis of worm population characteristics and 20 were designated for blood and faecal collection and for weighing. Two parasite-free tracer calves were grazed alongside the weaner calves each month throughout the study period and were also slaughtered for analysis of worm populations. Faecal egg counts, haematological and serum pepsinogen determinations, herbage larval counts, and animal live weight changes were recorded monthly. The study revealed that Haemonchus placei, Trichostrongylus axei, Cooperia spp. and Oesophagostomum radiatum were responsible for parasitic gastroenteritis and that H. placei was the predominant nematode present in the young cattle on both farms. Faecal egg counts from resident cattle and necropsy worm counts revealed that pasture larval levels were directly related to the amount of rainfall. The total worm burdens in the animals were highest during the rainy season (March–June and October–December) and lowest during the dry seasons (July–September and January–February). The very low recovery of immature larvae of H. placei from the tracer calves indicated that arrested development is not a feature of the life cycle of this parasite in central Kenya. The maintenance of the parasite population depended on continuous cycling of infection between the host and the pasture. The agroclimatic conditions of the study area were such that, in general, favourable weather conditions for the development and survival of the free-living stages of gastrointestinal nematodes existed all year round.  相似文献   

15.
The occurrence of ectoparasites in sheep flocks is frequently reported but seldom quantified. Sheep production used to be a predominantly family activity in the state of Sa~o Paulo (Brazil), but it began to become a commercial activity in the past decade. Thus, information about the ectoparasites existing in sheep flocks has become necessary. The present data were obtained by means of questionnaires sent to all sheep breeders belonging to the `Associaça~o Paulista de Criadores de Ovinos' (ASPACO; Sa~o Paulo State Association of Sheep Breeders). Response reliability was tested by means of random visits paid to 10.6% of the respondents. Most of the properties (89.5%) reported the presence of one or more ectoparasites. Screw-worm (Cochliomyia hominivorax) was the most frequent ectoparasite (72.5%), followed by bot fly larvae (Dermatobia hominis, 45.0%), ticks (Amblyomma cajennense) and Boophilus microplus, 31.3%) and finally lice (Damalinia ovis, 13.8%). Combined infestations also occurred, the most common one being screw-worm with bot fly larvae (36.0%) followed by bot fly larvae with ticks (13.9%), screw-worm with ticks (9.3%), bot fly larvae with lice (6.9%), and ticks with lice (5.0%). The most common triple combination was screw-worm, bot fly larvae and ticks (12.8%). Breeds raised for meat or wool were attacked by bot fly larvae and ticks more often than other breeds. Lice were only absent from animals of indigenous breeds. The relationships among these ectoparasites are discussed in terms of sheep breeds, flock size, seasonality and the ectoparasitic combinations on the host.  相似文献   

16.
17.
An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro‐organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.  相似文献   

18.
Serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in Shandan county of Gansu province. The liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. Most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. The liver contained the highest concentrations of copper, manganese and cobalt and the kidney of selenium, iron and calcium.Abbreviations Alb albumin - ALT alanine aminotransferase - AST aspartate aminotransferase - BUN blood urea nitrogen - Chol cholesterol - Glob globulin - -GT -glutamyl transpeptidase - IP inorganic phosphorus - TP total protein  相似文献   

19.
正We are pleased to publish the second special issue on avian brood parasitism and to be responsible guest editors for the two special issues of Chinese Birds (Vol. 3, No. 4, 2012 and Vol. 4, No. 1, 2013), entitled "Avian Brood Parasitism - A Growing Research Area in Behavioral Ecology". The first issue was published in December 2012. The goal of the two special issues is to publish accumulated knowledge and some of the recent developments in  相似文献   

20.
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