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己烯雌酚在动物食品中残留检测方法简述 总被引：11，自引：1，他引：10
己烯雌酚为一种具有酚羟基结构的人工合成雌激素 ,欧美等许多国家禁止将其用于动物饲料中 ,因此己烯雌酚在动物性食品中的残留普遍受到各国的高度重视。本文就己烯雌酚在动物性食品中的残留检测方法作了综述 ,着重介绍了放射免疫法 ,气相色谱法以及液相色谱法等检测方法。 相似文献
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己烯雌酚属于二苯乙烯类，并具有雌性激素的作用，是有效的促蛋白合成类固醇，在家禽或鱼类的肉产品中过量残留己烯雌酚会对消费者造成危害，为此己烯雌酚已经被禁止在食品生产中使用。但肉类食品中的过量残留，主要是由于被饲喂动物的饲料中含有高含量的己烯雌酚缓慢蓄集所致，所以要使食品中己烯雌酚得到有效控制， 相似文献
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己烯雌酚的临床应用、毒负作用及其残留检测方法研究进展 总被引：3，自引：0，他引：3
己烯雌酚(Diethylstilbestol，DES)是一种人工合成的非甾体类雌激素，具有酚羟基结构，其与天然雌激素的性质不同，应用之后，易在动物体内残留并产生严重的毒负作用。为此，欧美等许多国家已禁止在动物性饲料中添加己烯雌酚。本文就己烯雌酚的临床应用、毒负作用及其残留检测方法作一综述。 相似文献
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酶联免疫法检测饲料中己烯雌酚的探讨 总被引：1，自引：0，他引：1
己烯雌酚酶联免疫反应测试盒是用于饲料中己烯和其他相关的类固醇残留物如二氢己烯雌酚(hexe-strol)的定量检测。在家禽或鱼类中过量残留会对消费者造成危害,为此己烯雌酚已经被禁止在食品生产中使用。肉类食品中的过量残留,主要是由于被饲喂动物的饲料中含有高含量的己烯雌酚,动物缓慢蓄集所致,所以要想对食品中己烯雌酚得到有效控制,对饲料中的己烯雌酚进行监测至关重要。经实验,酶联免疫法具有操作简单、灵敏度高、时间短、检测成本低廉等优点,检测平均回收率≥98.3%,相关系数(r值)≥0.9992,离散系数(CV值)≤3.4%。 相似文献
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己烯雌酚属于二苯乙烯类,并具有雌性激素的作用,是有效的促蛋白合成类固醇,在家禽或鱼类的肉产品中过量残留己烯雌酚会对消费者造成危害,为此己烯雌酚已经被禁止在食品生产中使用。但肉类食品中的过量残留,主要是由于被饲喂动物的饲料中非法添加含高含量的己烯雌酚所致,所以要使食品中己烯雌酚得到有效控制,对饲料中的己烯雌酚进行监测也很重要。经试验:利用高效液相色谱串联质谱法(UPLC-MS/MS)检测饲料中己烯雌酚的含量,试样中的己烯雌酚经用甲醇溶解试样后,离心过膜后,采用负电喷雾离子源,在多反应监测(MRM)模式下进行分析,外标法定量,三对定性离子对分别为:m/z320.90>151.85、320.90>256.92和320.90>193.90,定量离子对为320.90>151.85。在1μg/L～500μg/L浓度范围内,呈良好的线性,相关系数≥0.99995。在鸡用配合饲料、鸡用浓缩饲料、鱼用配合饲料、鱼用浓缩饲料中,平均回收率≥93.0%,回收率标准偏差≤0.85%,检测限为0.01mg/kg,定量限为0.02mg/kg。该方法分析饲料中的己烯雌酚,前处理方法操作简便、快速、净化效果好,可同时定性和定量,方法的回收率、重复性、检测限均能满足饲料中己烯雌酚残留检测的要求。 相似文献
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V R Fajt 《Veterinary Clinics of North America: Food Animal Practice》2001,17(2):403-420
The small ruminant practitioner has a small arsenal of approved drugs in the United States, so the practitioner must be familiar with the laws and regulations related to extra label use. Drugs can be used extra label in food animals only under specific circumstances and can be used only for therapeutic purposes. Drugs that are illegal in small ruminants include chloramphenicol; clenbuterol; diethylstilbestrol; dimetridazole, ipranidazole, and other nitroimidazoles such as metronidazole; dipyrone; fluoroquinolones; glycopeptides; nitrofurans; furazolidone; and extra label use of medication in feed. It is also illegal to use any drug that results in residues above established tolerances or safe levels. 相似文献
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UPLC-MS/MS测定动物源性食品中3种二苯乙烯类激素残留 总被引：1，自引：1，他引：0
建立了动物源性食品中二苯乙烯类激素己烯雌酚、己烷雌酚和双烯雌酚残留的超高效液相色谱-串联质谱（UPLC-MS/MS）测定法。样品均质后,采用β-葡萄糖醛酸酶/芳基硫酸酯酶酶解,叔丁基甲基醚提取,硅胶固相萃取柱净化。采用UPLC-MS/MS多反应监测（MRM）模式检测,内标法定量。方法对己烯雌酚、己烷雌酚和双烯雌酚的检出限均为0.1μg/kg,定量限均为0.5μg/kg;在1.0、1.5、2.0μg/kg三个浓度添加水平,总体平均回收率为75.8%-110.0%,总体相对标准偏差为3.3%-13.2%。本法分析速度快,灵敏度高,重现性好,各项技术指标均满足国内外相关法规要求,可用于各种动物源性食品中二苯乙烯类激素己烯雌酚、己烷雌酚和双烯雌酚残留的快速检测。 相似文献
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The new combination of isocratic high performance liquid chromatography (HPLC) with on line UV spectrum detection via a diode array configuration has been applied to the detection and identification of anabolics present in application sites of cattle. Combination of the characteristic retention time in the HPLC chromatogram and a comparison of the full spectrum between 190-400 nm of the anabolic components with that of a standard resulted in a very reliable identification. By means of this method 117 samples of application sites were investigated for the presence of anabolic residues. Of the xenobiotic anabolics , 19-nortestosterone (NT) was found most frequently (in 96 cases), whereas diethylstilbestrol (DES) was found in only 11 cases. In all samples the identification of NT and DES was confirmed by high resolution gas chromatography-mass spectrometry (GCMS). 相似文献
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己烯雌酚人工抗原的合成及其抗体制备 总被引：5，自引：0，他引：5
以己烯雌酚 (DES)、琥珀酸酐和牛血清白蛋白 (BSA)等为主要原料 ,采用混合酸酐反应 ,首先合成己烯雌酚半琥珀酸酯 (DES- HS) ,经质谱分析鉴定后 ,再将 DES- HS结合到 BSA上 ,并用 SDS- PAGE对 DES- HS- BSA进行测定。以DES- HS- BSA免疫家兔 6次 ,用琼脂双扩散试验测定抗体。试验结果表明 ,高分辨质谱测得合成的 DES- HS分子量为36 8;BSA- HS- DES的 SDS- PAGE图谱经相关软件分析表明 ,单个 BSA上结合有 32个 DES- HS;琼脂扩散试验结果证实 ,免疫兔血清中含有抗 DES的抗体。本试验为下一步研制 DES残留免疫检测试剂盒及抗 DES单抗奠定了基础 相似文献
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R J Heitzman 《Journal of animal science》1983,57(1):233-238
The metabolic fate of the anabolic agents, diethylstilbestrol, hexestrol, trenbolone acetate, zeranol and the endogenous steroids are discussed under the headings absorption, distribution and excretion. There is an optimum concentration of anabolic agent in the systemic circulation that results in a maximum increase in growth rate of farm animals. This optimum blood concentration should be maintained over a long period. However, there is rapid metabolism and excretion of anabolic agents with short half-lives in blood, and metabolic clearance rate equals entry rate. The rate of absorption of the agent, which is determined by formulation and site of administration, is most important and is best achieved by the use of slow release implants. The pattern of exponential absorption from compressed pellets of single anabolic agents is not ideal, and a more constant payout of drug, in particular estradiol-17 beta, is best achieved in combined preparations of agents or from silicone rubber implants impregnated with the agent. The high metabolic clearance rate and rapid excretion of anabolic substances influences the distribution of residues. Outside the site of administration, less than 1% of the administered dose is present in the animal. The lowest concentrations of residues are found in muscle and fat, higher concentrations are present in liver and kidney and the highest concentrations are in the bile, urine and feces. 相似文献
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Summary The new combination of isocratic high performance liquid chromatography (HPLC) with on line UV spectrum detection via a diode array configuration has been applied to the detection and identification of anabolics present in application sites of cattle. Combination of the characteristic retention time in the HPLC chromatogram and a comparison of the full spectrum between 190–400 nm of the anabolic components with that of a standard resulted in a very reliable identification. By means of this method 117 samples of application sites were investigated for the presence of anabolic residues. Of the xenobiotic anabolics, 19‐nortestosterone (NT) was found most frequently (in 96 cases), whereas diethylstilbestrol (DES) was found in only 11 cases. In all samples the identification of NT and DES was confirmed by high resolution gas chromatography‐mass spectrometry (GCMS). 相似文献
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In order to develop a sensitive and specific method for the detection of estradiol residues (E2) in milk, a colloidal gold immunochromatographic method was established. Colloidal gold particles were prepared by trisodium citrate reduction method and labeled with rabbit polyclonal antibodies (PcAb) by physical adsorption method. After optimization of reaction conditions such as the amount of coating antigen and labeled antibody to assemble test strip, the sensitivity, specificity, repeatability and accelerated preservation of the test strip were determined. The estrogen analogue cross reaction and milk matrix interference reaction were also measured. The results showed that the optimized concentration of E2-OVA antigen was 2 mg/mL and the optimized antibody concentration was 20 μg/mL, visual detection limits of E2 was 10 μg/L in PBS within 10 min. The cross reaction rate of this method with estriol was 40%, there were no negligible cross-reactivities with other estrogen compounds including estradiol valerate, estradiol benzoate, estrone, diethylstilbestrol, quinestrol, ethinyloestradiol and nonylphenol. Milk samples only needed two times diluted before analysis, and the results could judge by naked eye after 10 min with cut-off of 20 μg/L. The results demonstrated that the developed method was suitable for rapid on-site screening of E2 residues in milk samples. 相似文献
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牛奶中雌二醇胶体金试纸条快速检测技术研究 总被引：1，自引：0，他引：1
为了建立快速、敏感、特异的雌二醇（E2）残留免疫检测方法,本研究应用胶体金免疫层析技术,研究制备一种快速检测牛奶中E2的方法。采用柠檬酸三钠还原法制备胶体金颗粒,采用物理吸附法将E2兔多克隆抗体偶联至胶体金,经过优化抗原包被量和多克隆抗体标记量等反应条件组装成检测试纸条,测定检测试纸的灵敏度、特异性、重复性和加速保存等参数,并对E2类似物交叉反应和牛奶基质干扰反应进行了测定。结果表明,试纸条最优包被抗原浓度为2 mg/mL,抗体浓度为20 μg/mL。采用消线法判定结果,检测时间10 min,PBS缓冲液中E2检测限为10 μg/L,该方法与雌三醇的交叉反应率为40%,与戊酸雌二醇、苯甲酸雌二醇、雌酮、己烯雌酚、炔雌醚、乙炔雌二醇、壬基酚等类似物均无可见交叉反应,牛奶样品经2倍稀释消除基质干扰直接用于检测,该方法在牛奶样品中的判定值为20 μg/L。本研究建立的E2胶体金试纸条使用简单方便,适合现场快速检测牛奶中E2残留。 相似文献